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List of works by Brigitte Gasser

A gene optimization strategy that enhances production of fully functional P-glycoprotein in Pichia pastoris

scientific article

A multi-level study of recombinant Pichia pastoris in different oxygen conditions.

scientific article

Accurate quantification of the redox-sensitive GSH/GSSG ratios in the yeast Pichia pastoris by HILIC-MS/MS.

scientific article published on 22 December 2012

Biomarkers allow detection of nutrient limitations and respective supplementation for elimination in Pichia pastoris fed-batch cultures.

scientific article

Characterization of methanol utilization negative Pichia pastoris for secreted protein production: New cultivation strategies for current and future applications

scientific article published on 08 February 2020

Creation of stable heterothallic strains of Komagataella phaffii enables dissection of mating gene regulation.

scientific article published on 23 October 2017

Curation of the genome annotation of Pichia pastoris (Komagataella phaffii) CBS7435 from gene level to protein function.

scientific article published on 6 July 2016

Directed gene copy number amplification in Pichia pastoris by vector integration into the ribosomal DNA locus.

scientific article

Disruption of genes involved in CORVET complex leads to enhanced secretion of heterologous carboxylesterase only in protease deficient Pichia pastoris.

scientific article published on 23 February 2017

Disruption of vacuolar protein sorting components of the HOPS complex leads to enhanced secretion of recombinant proteins in Pichia pastoris

scientific article published on 03 July 2019

Effects of gene dosage, promoters, and substrates on unfolded protein stress of recombinant Pichia pastoris

scientific article published in February 2004

Engineering of Pichia pastoris for improved production of antibody fragments.

scientific article published in June 2006

Engineering of alcohol dehydrogenase 2 hybrid-promoter architectures in Pichia pastoris to enhance recombinant protein expression on ethanol

scientific article published on 09 July 2019

Engineering of bottlenecks in Rhizopus oryzae lipase production in Pichia pastoris using the nitrogen source-regulated FLD1 promoter

scientific article published on 01 September 2009

Engineering of protein folding and secretion-strategies to overcome bottlenecks for efficient production of recombinant proteins

scientific article published on 24 March 2014

Functional inclusion bodies produced in the yeast Pichia pastoris.

scientific article published on October 2016

Genome, secretome and glucose transport highlight unique features of the protein production host Pichia pastoris.

scientific article

Genome-scale analysis of library sorting (GALibSo): Isolation of secretion enhancing factors for recombinant protein production in Pichia pastoris

scientific article

Genome-scale metabolic model of methylotrophic yeast Pichia pastoris and its use for in silico analysis of heterologous protein production.

scientific article published in July 2010

GoldenPiCS: a Golden Gate-derived modular cloning system for applied synthetic biology in the yeast Pichia pastoris

scientific article published on 8 December 2017

High level expression of a promising anti-idiotypic antibody fragment vaccine against HIV-1 in Pichia pastoris

article

Identification and characterization of the Komagataella phaffii mating pheromone genes.

scientific article

Identification and deletion of the major secreted protein of Pichia pastoris

scientific article

In Pichia pastoris, growth rate regulates protein synthesis and secretion, mating and stress response.

scientific article published on 14 January 2014

Increased dosage of AOX1 promoter-regulated expression cassettes leads to transcription attenuation of the methanol metabolism in Pichia pastoris

scientific article published on 15 March 2017

Increasing pentose phosphate pathway flux enhances recombinant protein production in Pichia pastoris.

scientific article published on 28 March 2016

Induction and measurement of UPR and osmotic stress in the yeast Pichia pastoris.

scientific article

Induction without methanol: novel regulated promoters enable high-level expression in Pichia pastoris.

scientific article published on 24 January 2013

Influence of growth temperature on the production of antibody Fab fragments in different microbes: a host comparative analysis.

scientific article published on 23 December 2010

Metabolic engineering of Pichia pastoris

scientific article published on 25 April 2018

Metabolomics sampling of Pichia pastoris revisited: rapid filtration prevents metabolite loss during quenching

scientific article published on 19 June 2015

Methanol regulated yeast promoters: production vehicles and toolbox for synthetic biology.

scientific article published on 2 December 2015

Model based engineering of Pichia pastoris central metabolism enhances recombinant protein production

scientific article

Molecular optimization of rabies virus glycoprotein expression in Pichia pastoris.

scientific article

Monitoring intracellular redox conditions in the endoplasmic reticulum of living yeasts

scientific article published on 22 February 2010

Monitoring of transcriptional regulation in Pichia pastoris under protein production conditions

scientific article

Multistep processing of the secretion leader of the extracellular protein Epx1 in Pichia pastoris and implications for protein localization

scientific article published in May 2015

Novel insights into the unfolded protein response using Pichia pastoris specific DNA microarrays.

scientific article

Open access to sequence: browsing the Pichia pastoris genome

scientific article

Oxidative protein folding and unfolded protein response elicit differing redox regulation in endoplasmic reticulum and cytosol of yeast

scientific article published on 8 March 2012

Pichia Pastoris : Protein Production Host and Model Organism for Biomedical Research ⬇️

scientific article published on February 1, 2013

Pichia pastoris Aft1--a novel transcription factor, enhancing recombinant protein secretion

scientific article published on 3 September 2014

Pichia pastoris regulates its gene-specific response to different carbon sources at the transcriptional, rather than the translational, level.

scientific article published on 11 March 2015

Pichia pastoris secretes recombinant proteins less efficiently than Chinese hamster ovary cells but allows higher space-time yields for less complex proteins

scientific article published on 27 January 2014

Production of recombinant proteins and metabolites in yeasts: when are these systems better than bacterial production systems?

scientific article

Protein folding and conformational stress in microbial cells producing recombinant proteins: a host comparative overview

scientific article published on 4 April 2008

Quo vadis? The challenges of recombinant protein folding and secretion in Pichia pastoris

scientific article published on 27 February 2015

Recombinant protein production 6 (Vienna, February 2011).

scientific article

Recombinant protein production in yeasts

scientific article published on 01 January 2012

Reverse engineering of protein secretion by uncoupling of cell cycle phases from growth

scientific article published on 25 May 2011

Sample preparation workflow for the liquid chromatography tandem mass spectrometry based analysis of nicotinamide adenine dinucleotide phosphate cofactors in yeast

scientific article published on 20 June 2014

Stress in recombinant protein producing yeasts.

scientific article published in September 2004

Superior protein titers in half the fermentation time: Promoter and process engineering for the glucose-regulated GTH1 promoter of Pichia pastoris

scientific article published on 08 August 2018

Systems biotechnology for protein production in Pichia pastoris.

scientific article published in November 2017

Systems-level organization of yeast methylotrophic lifestyle.

scientific article published on 23 September 2015

The bud tip is the cellular hot spot of protein secretion in yeasts

scientific article

The effect of temperature on the proteome of recombinant Pichia pastoris

scientific article published in March 2009

The impact of ERAD on recombinant protein secretion in Pichia pastoris (syn Komagataella spp.).

scientific article published on 13 March 2018

The industrial yeast Pichia pastoris is converted from a heterotroph into an autotroph capable of growth on CO2

scientific article published on 16 December 2019

The lipidome and proteome of microsomes from the methylotrophic yeast Pichia pastoris

scientific article published on 01 February 2014

The response to unfolded protein is involved in osmotolerance of Pichia pastoris.

scientific article

The secretome of Pichia pastoris in fed-batch cultivations is largely independent of the carbon source but changes quantitatively over cultivation time

scientific article published on 06 November 2019

The secretory pathway: exploring yeast diversity ⬇️

scientific article published on April 12, 2013

The vitamin-sensitive promoter PTHI11 enables pre-defined autonomous induction of recombinant protein production in Pichia pastoris.

scientific article published on 27 June 2016

Transcriptional engineering of the glyceraldehyde-3-phosphate dehydrogenase promoter for improved heterologous protein production in Pichia pastoris.

scientific article published on 26 June 2017

Transcriptomics-based identification of novel factors enhancing heterologous protein secretion in yeasts.

scientific article

U13C cell extract of Pichia pastoris--a powerful tool for evaluation of sample preparation in metabolomics

scientific article published on 22 October 2012

Unconventional microbial systems for the cost-efficient production of high-quality protein therapeutics.

scientific article published on 15 September 2012

Versatile modeling and optimization of fed batch processes for the production of secreted heterologous proteins with Pichia pastoris

scientific article published on 11 December 2006

Yeast biotechnology: teaching the old dog new tricks.

scientific article

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