List of works - Tip W. Loo

A salt bridge in intracellular loop 2 is essential for folding of human p-glycoprotein.

scientific article

ATP hydrolysis promotes interactions between the extracellular ends of transmembrane segments 1 and 11 of human multidrug resistance P-glycoprotein.

scientific article

Additive effect of multiple pharmacological chaperones on maturation of CFTR processing mutants

scientific article

Attachment of a 'molecular spring' restores drug-stimulated ATPase activity to P-glycoprotein lacking both Q loop glutamines.

scientific article published on 23 December 2016

Benzbromarone Stabilizes ΔF508 CFTR at the Cell Surface ⬇️

scientific article published on May 3, 2011

Chalcogenopyrylium Compounds as Modulators of the ATP-Binding Cassette Transporters P-Glycoprotein (P-gp/ABCB1) and Multidrug Resistance Protein 1 (MRP1/ABCC1)

article

Correction of Defective Protein Kinesis of Human P-glycoprotein Mutants by Substrates and Modulators

scientific article published in Journal of Biological Chemistry

Corrector VX-809 stabilizes the first transmembrane domain of CFTR.

scientific article published on 5 July 2013

Corrector-mediated rescue of misprocessed CFTR mutants can be reduced by the P-glycoprotein drug pump.

scientific article

Correctors Enhance Maturation of ΔF508 CFTR by Promoting Interactions between the Two Halves of the Molecule

scientific article published on 01 October 2009

Correctors promote folding of the CFTR in the endoplasmic reticulum.

scientific article

Correctors promote maturation of cystic fibrosis transmembrane conductance regulator (CFTR)-processing mutants by binding to the protein

scientific article published on 2 October 2007

Cross-linking of human multidrug resistance P-glycoprotein by the substrate, tris-(2-maleimidoethyl)amine, is altered by ATP hydrolysis. Evidence for rotation of a transmembrane helix.

scientific article

Cysteines introduced into extracellular loops 1 and 4 of human P-glycoprotein that are close only in the open conformation spontaneously form a disulfide bond that inhibits drug efflux and ATPase activity.

scientific article

Cystic fibrosis: channel, catalytic, and folding properties of the CFTR protein.

scientific article published on October 1997

Cytoplasmic loop three of cystic fibrosis transmembrane conductance regulator contributes to regulation of chloride channel activity.

scientific article published on November 1996

Defining the drug-binding site in the human multidrug resistance P-glycoprotein using a methanethiosulfonate analog of verapamil, MTS-verapamil

scientific article

Deletion of NH2 − and COOH-terminal sequences destroys function of the Ca2+ ATPase of rabbit fast-twitch skeletal muscle sarcoplasmic reticulum

article

Detection of antibodies to individual proteins of rubella virus

scientific article published in May 1986

Determining the dimensions of the drug-binding domain of human P-glycoprotein using thiol cross-linking compounds as molecular rulers

scientific article published on 22 August 2001

Disease-Associated Mutations in Cytoplasmic Loops 1 and 2 of Cystic Fibrosis Transmembrane Conductance Regulator Impede Processing or Opening of the Channel†

article

Disease-associated mutations in the fourth cytoplasmic loop of cystic fibrosis transmembrane conductance regulator compromise biosynthetic processing and chloride channel activity.

scientific article published on June 1996

Do drug substrates enter the common drug-binding pocket of P-glycoprotein through "gates"?

scientific article published in April 2005

Drug binding in human P-glycoprotein causes conformational changes in both nucleotide-binding domains.

scientific article published on 5 November 2002

Drug rescue distinguishes between different structural models of human P-glycoprotein

scientific article published on 2 October 2013

Drug-stimulated ATPase Activity of Human P-glycoprotein Requires Movement between Transmembrane Segments 6 and 12

scientific article published in Journal of Biological Chemistry

Drug-stimulated ATPase activity of human P-glycoprotein is blocked by disulfide cross-linking between the nucleotide-binding sites.

scientific article

Drugs Modulate Interactions between the First Nucleotide-Binding Domain and the Fourth Cytoplasmic Loop of Human P-Glycoprotein

scientific article published on 11 May 2016

Expression of a Functionally Active Human Renal Sodium-Calcium Exchanger Lacking a Signal Sequence ⬇️

scientific article published on August 18, 1995

Expression of rubella virus cDNA coding for the structural proteins

scientific article

Human P-glycoprotein contains a greasy ball-and-socket joint at the second transmission interface.

scientific article published on 03 June 2013

Human P-glycoprotein is active when the two halves are clamped together in the closed conformation

scientific article published on 13 April 2010

Identification of Residues in the Drug-binding Domain of Human P-glycoprotein

scientific article published in Journal of Biological Chemistry

Identification of Residues in the Drug-binding Site of Human P-glycoprotein Using a Thiol-reactive Substrate

scientific article published in Journal of Biological Chemistry

Identification of Residues within the Drug-binding Domain of the Human Multidrug Resistance P-glycoprotein by Cysteine-scanning Mutagenesis and Reaction with Dibromobimane

scientific article published in Journal of Biological Chemistry

Identification of the distance between the homologous halves of P-glycoprotein that triggers the high/low ATPase activity switch.

scientific article published on 12 February 2014

Inhibition of Oxidative Cross-linking between Engineered Cysteine Residues at Positions 332 in Predicted Transmembrane Segments (TM) 6 and 975 in Predicted TM12 of Human P-glycoprotein by Drug Substrates

scientific article published in Journal of Biological Chemistry

Interaction of Escherichia coli F1-ATPase with dicyclohexylcarbodiimide-binding polypeptide

scientific article published on 01 September 1983

Introduction of the most common cystic fibrosis mutation (Delta F508) into human P-glycoprotein disrupts packing of the transmembrane segments.

scientific article published on 17 June 2002

Location of the rhodamine-binding site in the human multidrug resistance P-glycoprotein.

scientific article published on 9 September 2002

Locking intracellular helices 2 and 3 together inactivates human P-glycoprotein.

scientific article published on 25 November 2013

Mapping the Binding Site of the Inhibitor Tariquidar That Stabilizes the First Transmembrane Domain of P-glycoprotein.

scientific article published on 26 October 2015

Modulating the Folding of P-Glycoprotein and Cystic Fibrosis Transmembrane Conductance Regulator Truncation Mutants with Pharmacological Chaperones

scientific article published on 28 November 2006

Mutational Analysis of the Predicted First Transmembrane Segment of Each Homologous Half of Human P-glycoprotein Suggests That They Are Symmetrically Arranged in the Membrane

scientific article published in Journal of Biological Chemistry

Mutational analysis of human P-glycoprotein.

scientific article published on January 1998

Niemann-Pick NPC1: sterols to the rescue and beyond

scientific article

Nonylphenol Ethoxylates, but Not Nonylphenol, Are Substrates of the Human Multidrug Resistance P-glycoprotein ⬇️

scientific article published on June 18, 1998

Nucleotide sequence and in vitro expression of rubella virus 24S subgenomic messenger RNA encoding the structural proteins E1, E2 and C

scientific article

P-glycoprotein ATPase activity requires lipids to activate a switch at the first transmission interface.

scientific article published on 2 March 2016

Permanent Activation of the Human P-glycoprotein by Covalent Modification of a Residue in the Drug-binding Site ⬇️

scientific article published on April 23, 2003

Predicting P-glycoprotein-mediated drug transport based on support vector machine and three-dimensional crystal structure of P-glycoprotein

scientific article

Processing mutations located throughout the human multidrug resistance P-glycoprotein disrupt interactions between the nucleotide binding domains.

scientific article published on 9 July 2004

Quality control by proteases in the endoplasmic reticulum. Removal of a protease-sensitive site enhances expression of human P-glycoprotein.

scientific article published on December 1998

Rapid purification of human P-glycoprotein mutants expressed transiently in HEK 293 cells by nickel-chelate chromatography and characterization of their drug-stimulated ATPase activities.

scientific article

Recent progress in understanding the mechanism of P-glycoprotein-mediated drug efflux.

scientific article

Repair of CFTR Folding Defects with Correctors that Function as Pharmacological Chaperones

scientific article published on 01 January 2011

Simultaneous binding of two different drugs in the binding pocket of the human multidrug resistance P-glycoprotein

scientific article published on 7 August 2003

Structural analysis of a new GC-specific insertion element IS186.

scientific article published in November 1985

Substrate-induced conformational changes in the transmembrane segments of human P-glycoprotein. Direct evidence for the substrate-induced fit mechanism for drug binding.

scientific article published on 27 February 2003

Superfolding of the partially unfolded core-glycosylated intermediate of human P-glycoprotein into the mature enzyme is promoted by substrate-induced transmembrane domain interactions.

scientific article published on June 1998

Suppressor mutations in the transmembrane segments of P-glycoprotein promote maturation of processing mutants and disrupt a subset of drug-binding sites.

scientific article published on 11 September 2007

Tariquidar inhibits P-glycoprotein drug efflux but activates ATPase activity by blocking transition to an open conformation

scientific article

The "LSGGQ" motif in each nucleotide-binding domain of human P-glycoprotein is adjacent to the opposing walker A sequence

scientific article published on 10 September 2002

The ATPase activity of the P-glycoprotein drug pump is highly activated when the N-terminal and central regions of the nucleotide-binding domains are linked closely together

scientific article

The DCCD-binding polypeptide alone is insufficient for proton translocation through F0 in membranes of Escherichia, coli

scientific article published on 01 November 1981

The DCCD-binding polypeptide is close to the F1 ATPase-binding site on the cytoplasmic surface of the cell membrane of Escherichia coli

scientific article published on 01 May 1982

The Transmission Interfaces Contribute Asymmetrically to the Assembly and Activity of Human P-glycoprotein

scientific article published on 18 May 2015

The V510D suppressor mutation stabilizes DeltaF508-CFTR at the cell surface.

scientific article published on August 2010

The W232R suppressor mutation promotes maturation of a truncation mutant lacking both nucleotide-binding domains and restores interdomain assembly and activity of P-glycoprotein processing mutants.

scientific article published on 11 January 2011

The chemical chaperone CFcor-325 repairs folding defects in the transmembrane domains of CFTR-processing mutants.

scientific article published on May 2006

The cystic fibrosis V232D mutation inhibits CFTR maturation by disrupting a hydrophobic pocket rather than formation of aberrant interhelical hydrogen bonds.

scientific article published on 9 January 2014

The drug-binding pocket of the human multidrug resistance P-glycoprotein is accessible to the aqueous medium.

scientific article published on September 2004

The glycosylation and orientation in the membrane of the third cytoplasmic loop of human P-glycoprotein is affected by mutations and substrates.

scientific article published on April 1999

The minimum functional unit of human P-glycoprotein appears to be a monomer.

scientific article published on November 1996

The packing of the transmembrane segments of human multidrug resistance P-glycoprotein is revealed by disulfide cross-linking analysis.

scientific article

The transmembrane domains of the human multidrug resistance P-glycoprotein are sufficient to mediate drug binding and trafficking to the cell surface.

scientific article published on August 1999

Thiorhodamines containing amide and thioamide functionality as inhibitors of the ATP-binding cassette drug transporter P-glycoprotein (ABCB1).

scientific article

Using a cysteine-less mutant to provide insight into the structure and mechanism of CFTR.

scientific article published on 23 February 2006

Val133 and Cys137 in transmembrane segment 2 are close to Arg935 and Gly939 in transmembrane segment 11 of human P-glycoprotein

scientific article published on 28 January 2004

Vanadate trapping of nucleotide at the ATP-binding sites of human multidrug resistance P-glycoprotein exposes different residues to the drug-binding site

scientific article

List of works by Tip W. Loo

A salt bridge in intracellular loop 2 is essential for folding of human p-glycoprotein.

ATP hydrolysis promotes interactions between the extracellular ends of transmembrane segments 1 and 11 of human multidrug resistance P-glycoprotein.

Additive effect of multiple pharmacological chaperones on maturation of CFTR processing mutants

Attachment of a 'molecular spring' restores drug-stimulated ATPase activity to P-glycoprotein lacking both Q loop glutamines.

Benzbromarone Stabilizes ΔF508 CFTR at the Cell Surface ⬇️

Chalcogenopyrylium Compounds as Modulators of the ATP-Binding Cassette Transporters P-Glycoprotein (P-gp/ABCB1) and Multidrug Resistance Protein 1 (MRP1/ABCC1)

Correction of Defective Protein Kinesis of Human P-glycoprotein Mutants by Substrates and Modulators

Corrector VX-809 stabilizes the first transmembrane domain of CFTR.

Corrector-mediated rescue of misprocessed CFTR mutants can be reduced by the P-glycoprotein drug pump.

Correctors Enhance Maturation of ΔF508 CFTR by Promoting Interactions between the Two Halves of the Molecule

Correctors promote folding of the CFTR in the endoplasmic reticulum.

Correctors promote maturation of cystic fibrosis transmembrane conductance regulator (CFTR)-processing mutants by binding to the protein

Cross-linking of human multidrug resistance P-glycoprotein by the substrate, tris-(2-maleimidoethyl)amine, is altered by ATP hydrolysis. Evidence for rotation of a transmembrane helix.

Cysteines introduced into extracellular loops 1 and 4 of human P-glycoprotein that are close only in the open conformation spontaneously form a disulfide bond that inhibits drug efflux and ATPase activity.

Cystic fibrosis: channel, catalytic, and folding properties of the CFTR protein.

Cytoplasmic loop three of cystic fibrosis transmembrane conductance regulator contributes to regulation of chloride channel activity.

Defining the drug-binding site in the human multidrug resistance P-glycoprotein using a methanethiosulfonate analog of verapamil, MTS-verapamil

Deletion of NH2 − and COOH-terminal sequences destroys function of the Ca2+ ATPase of rabbit fast-twitch skeletal muscle sarcoplasmic reticulum

Detection of antibodies to individual proteins of rubella virus

Determining the dimensions of the drug-binding domain of human P-glycoprotein using thiol cross-linking compounds as molecular rulers

Disease-Associated Mutations in Cytoplasmic Loops 1 and 2 of Cystic Fibrosis Transmembrane Conductance Regulator Impede Processing or Opening of the Channel†

Disease-associated mutations in the fourth cytoplasmic loop of cystic fibrosis transmembrane conductance regulator compromise biosynthetic processing and chloride channel activity.

Do drug substrates enter the common drug-binding pocket of P-glycoprotein through "gates"?

Drug binding in human P-glycoprotein causes conformational changes in both nucleotide-binding domains.

Drug rescue distinguishes between different structural models of human P-glycoprotein

Drug-stimulated ATPase Activity of Human P-glycoprotein Requires Movement between Transmembrane Segments 6 and 12

Drug-stimulated ATPase activity of human P-glycoprotein is blocked by disulfide cross-linking between the nucleotide-binding sites.

Drugs Modulate Interactions between the First Nucleotide-Binding Domain and the Fourth Cytoplasmic Loop of Human P-Glycoprotein

Expression of a Functionally Active Human Renal Sodium-Calcium Exchanger Lacking a Signal Sequence ⬇️

Expression of rubella virus cDNA coding for the structural proteins

Human P-glycoprotein contains a greasy ball-and-socket joint at the second transmission interface.

Human P-glycoprotein is active when the two halves are clamped together in the closed conformation

Identification of Residues in the Drug-binding Domain of Human P-glycoprotein

Identification of Residues in the Drug-binding Site of Human P-glycoprotein Using a Thiol-reactive Substrate

Identification of Residues within the Drug-binding Domain of the Human Multidrug Resistance P-glycoprotein by Cysteine-scanning Mutagenesis and Reaction with Dibromobimane

Identification of the distance between the homologous halves of P-glycoprotein that triggers the high/low ATPase activity switch.

Inhibition of Oxidative Cross-linking between Engineered Cysteine Residues at Positions 332 in Predicted Transmembrane Segments (TM) 6 and 975 in Predicted TM12 of Human P-glycoprotein by Drug Substrates

Interaction of Escherichia coli F1-ATPase with dicyclohexylcarbodiimide-binding polypeptide

Introduction of the most common cystic fibrosis mutation (Delta F508) into human P-glycoprotein disrupts packing of the transmembrane segments.

Location of the rhodamine-binding site in the human multidrug resistance P-glycoprotein.

Locking intracellular helices 2 and 3 together inactivates human P-glycoprotein.

Mapping the Binding Site of the Inhibitor Tariquidar That Stabilizes the First Transmembrane Domain of P-glycoprotein.

Modulating the Folding of P-Glycoprotein and Cystic Fibrosis Transmembrane Conductance Regulator Truncation Mutants with Pharmacological Chaperones

Mutational Analysis of the Predicted First Transmembrane Segment of Each Homologous Half of Human P-glycoprotein Suggests That They Are Symmetrically Arranged in the Membrane

Mutational analysis of human P-glycoprotein.

Niemann-Pick NPC1: sterols to the rescue and beyond

Nonylphenol Ethoxylates, but Not Nonylphenol, Are Substrates of the Human Multidrug Resistance P-glycoprotein ⬇️

Nucleotide sequence and in vitro expression of rubella virus 24S subgenomic messenger RNA encoding the structural proteins E1, E2 and C

P-glycoprotein ATPase activity requires lipids to activate a switch at the first transmission interface.

Permanent Activation of the Human P-glycoprotein by Covalent Modification of a Residue in the Drug-binding Site ⬇️

Predicting P-glycoprotein-mediated drug transport based on support vector machine and three-dimensional crystal structure of P-glycoprotein

Processing mutations located throughout the human multidrug resistance P-glycoprotein disrupt interactions between the nucleotide binding domains.

Quality control by proteases in the endoplasmic reticulum. Removal of a protease-sensitive site enhances expression of human P-glycoprotein.

Rapid purification of human P-glycoprotein mutants expressed transiently in HEK 293 cells by nickel-chelate chromatography and characterization of their drug-stimulated ATPase activities.

Recent progress in understanding the mechanism of P-glycoprotein-mediated drug efflux.

Repair of CFTR Folding Defects with Correctors that Function as Pharmacological Chaperones

Simultaneous binding of two different drugs in the binding pocket of the human multidrug resistance P-glycoprotein

Structural analysis of a new GC-specific insertion element IS186.

Substrate-induced conformational changes in the transmembrane segments of human P-glycoprotein. Direct evidence for the substrate-induced fit mechanism for drug binding.

Superfolding of the partially unfolded core-glycosylated intermediate of human P-glycoprotein into the mature enzyme is promoted by substrate-induced transmembrane domain interactions.

Suppressor mutations in the transmembrane segments of P-glycoprotein promote maturation of processing mutants and disrupt a subset of drug-binding sites.

Tariquidar inhibits P-glycoprotein drug efflux but activates ATPase activity by blocking transition to an open conformation

The "LSGGQ" motif in each nucleotide-binding domain of human P-glycoprotein is adjacent to the opposing walker A sequence

The ATPase activity of the P-glycoprotein drug pump is highly activated when the N-terminal and central regions of the nucleotide-binding domains are linked closely together

The DCCD-binding polypeptide alone is insufficient for proton translocation through F0 in membranes of Escherichia, coli

The DCCD-binding polypeptide is close to the F1 ATPase-binding site on the cytoplasmic surface of the cell membrane of Escherichia coli

The Transmission Interfaces Contribute Asymmetrically to the Assembly and Activity of Human P-glycoprotein

The V510D suppressor mutation stabilizes DeltaF508-CFTR at the cell surface.

The W232R suppressor mutation promotes maturation of a truncation mutant lacking both nucleotide-binding domains and restores interdomain assembly and activity of P-glycoprotein processing mutants.

The chemical chaperone CFcor-325 repairs folding defects in the transmembrane domains of CFTR-processing mutants.

The cystic fibrosis V232D mutation inhibits CFTR maturation by disrupting a hydrophobic pocket rather than formation of aberrant interhelical hydrogen bonds.

The drug-binding pocket of the human multidrug resistance P-glycoprotein is accessible to the aqueous medium.

The glycosylation and orientation in the membrane of the third cytoplasmic loop of human P-glycoprotein is affected by mutations and substrates.

The minimum functional unit of human P-glycoprotein appears to be a monomer.

The packing of the transmembrane segments of human multidrug resistance P-glycoprotein is revealed by disulfide cross-linking analysis.

The transmembrane domains of the human multidrug resistance P-glycoprotein are sufficient to mediate drug binding and trafficking to the cell surface.

Thiorhodamines containing amide and thioamide functionality as inhibitors of the ATP-binding cassette drug transporter P-glycoprotein (ABCB1).

Using a cysteine-less mutant to provide insight into the structure and mechanism of CFTR.

Val133 and Cys137 in transmembrane segment 2 are close to Arg935 and Gly939 in transmembrane segment 11 of human P-glycoprotein