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List of works by Shigeaki Harayama

4-Oxalocrotonate tautomerase, an enzyme composed of 62 amino acid residues per monomer ⬇️

scientific article (publication date: 5 September 1992)

A Novel Nuclear Export Inhibitor JBIR-02, a New Piericidin Discovered from Streptomyces sp. ML55 ⬇️

scientific article published on 19 January 2009

A high-throughput and generic assay method for the determination of substrate specificities of thermophilic alpha-aminotransferases

scientific article published on 21 July 2007

A novel phenanthrene dioxygenase from Nocardioides sp. Strain KP7: expression in Escherichia coli.

scientific article published on April 2000

A simple and rapid GC/MS method for the simultaneous determination of gaseous metabolites

scientific article published on 31 October 2010

A simple procedure for transferring genes cloned in Escherichia coli vectors into other gram-negative bacteria: phenotypic analysis and mapping of TOL plasmid gene xylK.

scientific article

Acanthopleuribacter pedis gen. nov., sp. nov., a marine bacterium isolated from a chiton, and description of Acanthopleuribacteraceae fam. nov., Acanthopleuribacterales ord. nov., Holophagaceae fam. nov., Holophagales ord. nov. and Holophagae classi

scientific article

Activity and population structure of nitrifying bacteria in an activated-sludge reactor containing polymer beads.

scientific article published in April 2003

Adhesive protein cDNA sequence of the mussel Mytilus coruscus and its evolutionary implications

scientific article published in October 1996

Advances in the field of high-molecular-weight polycyclic aromatic hydrocarbon biodegradation by bacteria

scientific article (publication date: March 2010)

Alcanivorax which prevails in oil-contaminated seawater exhibits broad substrate specificity for alkane degradation

scientific article published in September 2003

Alkane-degrading properties of Dietzia sp. H0B, a key player in the Prestige oil spill biodegradation (NW Spain).

scientific article

An AraC/XylS family member at a high level in a hierarchy of regulators for phenol-metabolizing enzymes in Comamonas testosteroni R5

scientific article published on July 2002

An effective family shuffling method using single-stranded DNA

scientific article published on 01 February 2000

An outbreak of nonflocculating catabolic populations caused the breakdown of a phenol-digesting activated-sludge process.

scientific article

Analysis of long-side-chain alkylaromatics in crude oil for evaluation of their fate in the environment

scientific article

Artificial evolution by DNA shuffling.

scientific article

Bacterial aromatic ring-cleavage enzymes are classified into two different gene families.

scientific article published in September 1989

Bacterial populations occuring in a trichloroethylene-contaminated aquifer during methane injection.

scientific article published in March 2001

Barnacle cement proteins. Importance of disulfide bonds in their insolubility

scientific article published in September 2000

Biochemical characterization of a Ca(2+)-dependent lectin from the hemolymph of a photosymbiotic marine bivalve, Tridacna derasa (Röding)

scientific article published on 01 May 1995

Biodegradation of n-alkylcycloalkanes and n-alkylbenzenes via new pathways in Alcanivorax sp. strain MBIC 4326.

scientific article

Biofilm-based photobioreactor absorbing water and nutrients by capillary action

scientific article published on 31 October 2016

Biotransformation of phenanthrene and 1-methoxynaphthalene with Streptomyces lividans cells expressing a marine bacterial phenanthrene dioxygenase gene cluster.

scientific article

Characteristics of transposon insertion mutants of mandelic acid-utilizing Pseudomonas putida strain A10L

scientific article published on 01 July 1996

Characterization of a high-affinity phenol hydroxylase from Comamonas testosteroni R5 by gene cloning, and expression in Pseudomonas aeruginosa PAO1c

scientific article published in October 1999

Characterization of four Rhodococcus alcohol dehydrogenase genes responsible for the oxidation of aromatic alcohols

scientific article

Characterization of genes for enzymes involved in the phenanthrene degradation in Nocardioides sp. KP7.

scientific article published in March 1999

Characterization of light-harvesting mutants of Rhodopseudomonas sphaeroides. I. Measurement of the efficiency of energy transfer from light-harvesting complexes to the reaction center

scientific article published on 01 January 1985

Characterization of the mgl operon of Escherichia coli by transposon mutagenesis and molecular cloning.

scientific article

Cloning and functional analysis of alkB genes in Alcanivorax borkumensis SK2.

scientific article published in March 2004

Cloning and nucleotide sequence of the gyrB gene of Vibrio parahaemolyticus and its application in detection of this pathogen in shrimp.

scientific article

Cloning of trg, a gene for a sensory transducer in Escherichia coli.

scientific article

Cloning, sequencing and sites of expression of genes for the hydroxyarginine-containing adhesive-plaque protein of the mussel Mytilus galloprovincialis

scientific article published in July 1996

Cloning, sequencing, and expression of the Pseudomonas testosteroni gene encoding 3-oxosteroid delta 1-dehydrogenase ⬇️

scientific article published on November 1, 1991

Codon usage patterns suggest independent evolution of two catabolic operons on toluene-degradative plasmid TOL pWW0 of Pseudomonas putida.

scientific article published in April 1994

Comparison of the nucleotide sequences of the meta-cleavage pathway genes of TOL plasmid pWW0 from Pseudomonas putida with other meta-cleavage genes suggests that both single and multiple nucleotide substitutions contribute to enzyme evolution

scientific article published in May 1993

Complete genome sequence of the soil actinomycete Kocuria rhizophila

scientific article

Conservative Structure of the Plaque Matrix Protein of Mussels in the Genus Mytilus

scientific article published on 01 July 2000

Construction of Marker-Free Transgenic Strains of Chlamydomonas reinhardtii Using a Cre/loxP-Mediated Recombinase System

scientific article

Construction of chimeric catechol 2,3-dioxygenase exhibiting improved activity against the suicide inhibitor 4-methylcatechol.

scientific article published in March 2004

Construction of the gyrB Database for the Identification and Classification of Bacteria

scientific article published on 01 January 1998

Conversion of pBR322-based plasmids into broad-host-range vectors by using the Tn3 transposition mechanism.

scientific article

Corrosion of iron by iodide-oxidizing bacteria isolated from brine in an iodine production facility

scientific article published on 27 May 2014

Cosmid cloning and transposon mutagenesis in Salmonella typhimurium using phage lambda vehicles

scientific article published in January 1981

Crystal ball

scientific article published on 01 February 2000

DNA sequence determination of the TOL plasmid (pWW0) xylGFJ genes of Pseudomonas putida: implications for the evolution of aromatic catabolism ⬇️

scientific article published on October 1, 1991

DNA shuffling and family shuffling for in vitro gene evolution

article

Development of specific oligonucleotide probes for the identification and in situ detection of hydrocarbon-degrading Alcanivorax strains

scientific article published in June 2001

Divergent evolution of chloroplast-type ferredoxins

scientific article published on 01 July 1991

Diversity in kinetics of trichloroethylene-degrading activities exhibited by phenol-degrading bacteria

scientific article published in March 2001

Does Tn10 transpose via the cointegrate molecule?

scientific article

Effect of motility and chemotaxis on the invasion of Salmonella typhimurium into HeLa cells

scientific article published in July 1990

Effects of chloramphenicol on photosynthesis, protein profiles and transketolase activity under extremely high CO2 concentration in an extremely-high-CO2-tolerant green microalga, Chlorococcum littorale

scientific article published in December 2004

Energy is required for maturation of exported proteins in Escherichia coli

scientific article published on 01 May 1981

Enhanced mineralization of benzo[a]pyrene in the presence of nonaqueous phase liquids

scientific article

Euzebya tangerina gen. nov., sp. nov., a deeply branching marine actinobacterium isolated from the sea cucumber Holothuria edulis, and proposal of Euzebyaceae fam. nov., Euzebyales ord. nov. and Nitriliruptoridae subclassis nov.

scientific article

Evolutionary relationships between catabolic pathways for aromatics: conservation of gene order and nucleotide sequences of catechol oxidation genes of pWW0 and NAH7 plasmids

scientific article published in December 1987

Extracellular polysaccharides of Rhodococcus rhodochrous S-2 stimulate the degradation of aromatic components in crude oil by indigenous marine bacteria.

scientific article

Functional and evolutionary relationships among diverse oxygenases

scientific article published on January 1, 1992

Functional and structural analyses of trichloroethylene-degrading bacterial communities under different phenol-feeding conditions: laboratory experiments

scientific article published on 14 December 2002

Genetic transformation of Pseudochoricystis ellipsoidea, an aliphatic hydrocarbon-producing green alga

scientific article published in January 2012

High frequency mobilization of the chromosome of Escherichia coli by a mutant of plasmid RP4 temperature-sensitive for maintenance.

scientific article published in January 1980

ICB database: the gyrB database for identification and classification of bacteria.

scientific article

Iamia majanohamensis gen. nov., sp. nov., an actinobacterium isolated from sea cucumber Holothuria edulis, and proposal of Iamiaceae fam. nov.

scientific article

Identification and characterization of o-xylene-degrading Rhodococcus spp. which were dominant species in the remediation of o-xylene-contaminated soils

scientific article published on 17 February 2006

Identification by the phage-display technique of peptides that bind to H7 flagellin of Escherichia coli.

scientific article

Ignavibacterium album gen. nov., sp. nov., a moderately thermophilic anaerobic bacterium isolated from microbial mats at a terrestrial hot spring and proposal of Ignavibacteria classis nov., for a novel lineage at the periphery of green sulfur bacte

scientific article published on 11 August 2009

Induction kinetics of RNA and proteins in exponentially growing organisms

scientific article published in October 1991

Influence of extracellular polysaccharides (EPS) produced by two different green unicellular algae on membrane filtration in an algae-based biofuel production process

scientific article published in January 2014

Interspecific variations in adhesive protein sequences of Mytilus edulis, M. galloprovincialis, and M. trossulus

scientific article published in December 1995

Intersubunit interaction and catalytic activity of catechol 2,3-dioxygenases.

scientific article

Intrageneric relationships among Micromonospora species deduced from gyrB-based phylogeny and DNA relatedness

scientific article published in January 2000

Iron corrosion activity of anaerobic hydrogen-consuming microorganisms isolated from oil facilities

scientific article published on 26 May 2010

Iron corrosion induced by nonhydrogenotrophic nitrate-reducing Prolixibacter sp. strain MIC1-1.

scientific article

Isolation and characterization of thermophilic bacilli degrading cinnamic, 4-coumaric, and ferulic acids.

scientific article

Isolation and phylogenetic characterization of bacteria capable of inducing differentiation in the green alga Monostroma oxyspermum

scientific article published in January 2003

Kinetic studies on benzyl alcohol dehydrogenase encoded by TOL plasmid pWWO. A member of the zinc-containing long chain alcohol dehydrogenase family.

scientific article published on May 1993

Krokinobacter gen. nov., with three novel species, in the family Flavobacteriaceae.

scientific article

Lacticigenium naphtae gen. nov., sp. nov., a halotolerant and motile lactic acid bacterium isolated from crude oil.

scientific article

Major Carotenoid Isolated fromParacoccus schoiniaNBRC 100637TIs Adonixanthin Diglucoside

scientific article published on 07 December 2006

Methanobacterium petrolearium sp. nov. and Methanobacterium ferruginis sp. nov., mesophilic methanogens isolated from salty environments

scientific article

Method for selective isolation of Mycobacteria using olive oil emulsified with SDS

scientific article published on 01 June 2007

Mobilicoccus pelagius gen. nov., sp. nov. and Piscicoccus intestinalis gen. nov., sp. nov., two new members of the family Dermatophilaceae, and reclassification of Dermatophilus chelonae (Masters et al. 1995) as Austwickia chelonae gen. nov., comb.

scientific article published on 01 January 2010

Molecular breeding of 2,3-dihydroxybiphenyl 1,2-dioxygenase for enhanced resistance to 3-chlorocatechol

scientific article published in March 2004

Molecular characterization and substrate preference of a polycyclic aromatic hydrocarbon dioxygenase from Cycloclasticus sp. strain A5

scientific article (publication date: November 2003)

Molecular cloning and characterization of genes required for ribose transport and utilization in Escherichia coli K-12 ⬇️

scientific article published on May 1, 1984

Molecular detection of marine bacterial populations on beaches contaminated by the Nakhodka tanker oil-spill accident.

scientific article

Molecular mechanisms of genetic adaptation to xenobiotic compounds ⬇️

scientific article published on December 1, 1992

Mutants in transmission of chemotactic signals from two independent receptors of E. coli ⬇️

scientific article published on March 1, 1979

Nocardia amamiensis sp. nov., isolated from a sugar-cane field in Japan

scientific article

Novel family shuffling methods for the in vitro evolution of enzymes

scientific article published on 01 August 1999

Nucleotide and deduced amino acid sequence of the RpoN sigma-factor of Pseudomonas putida.

scientific article published on December 1989

Oleibacter marinus gen. nov., sp. nov., a bacterium that degrades petroleum aliphatic hydrocarbons in a tropical marine environment

scientific article

Overexpression of the genes from thermophiles in Escherichia coli by high-temperature cultivation.

scientific article published on 4 July 2006

Oxygenation reactions of various tricyclic fused aromatic compounds using Escherichia coli and Streptomyces lividans transformants carrying several arene dioxygenase genes.

scientific article published in November 2001

PCR isolation of catechol 2,3-dioxygenase gene fragments from environmental samples and their assembly into functional genes.

scientific article

Paracoccus marinus sp. nov., an adonixanthin diglucoside-producing bacterium isolated from coastal seawater in Tokyo Bay.

scientific article

Paraferrimonas sedimenticola gen. nov., sp. nov., a marine bacterium of the family Ferrimonadaceae

scientific article

Paraoerskovia marina gen. nov., sp. nov., an actinobacterium isolated from marine sediment.

scientific article

Petroleum biodegradation in marine environments.

scientific article

PhcS represses gratuitous expression of phenol-metabolizing enzymes in Comamonas testosteroni R5.

scientific article

Photo-oxidation of biodegraded crude oil and toxicity of the photo-oxidized products.

scientific article

Phototactic response of aerobically cultivated Rhodospirillum rubrum

scientific article published on 01 May 1976

Phototaxis and membrane potential in the photosynthetic bacterium Rhodospirillum rubrum ⬇️

scientific article published on July 1, 1977

Phycisphaera mikurensis gen. nov., sp. nov., isolated from a marine alga, and proposal of Phycisphaeraceae fam. nov., Phycisphaerales ord. nov. and Phycisphaerae classis nov. in the phylum Planctomycetes

scientific article published in August 2009

Phylogenetic Analysis of Acinetobacter Strains Based on the Nucleotide Sequences of gyrB Genes and on the Amino Acid Sequences of Their Products

scientific article

Phylogenetic analysis of genus Marinilabilia and related bacteria based on the amino acid sequences of GyrB and emended description of Marinilabilia salmonicolor with Marinilabilia agarovorans as its subjective synonym

scientific article published in October 1999

Phylogenetic relationships of Pseudomonas putida strains deduced from the nucleotide sequences of gyrB, rpoD and 16S rRNA genes

scientific article

Phylogenetic structures of the genus Acinetobacter based on gyrB sequences: comparison with the grouping by DNA-DNA hybridization

scientific article

Phylogenetic study of the genus Oceanospirillum based on 16S rRNA and gyrB genes: emended description of the genus Oceanospirillum, description of Pseudospirillum gen. nov., Oceanobacter gen. nov. and Terasakiella gen. nov. and transfer of Oceanos...

scientific article published in May 2002

Phylogeny of the genus Pseudomonas: intrageneric structure reconstructed from the nucleotide sequences of gyrB and rpoD genes.

scientific article published in October 2000

Physicochemical characters of a tyrosinase inhibitor produced by Streptomyces roseolilacinus NBRC 12815.

scientific article

Potential DNA slippage structures acquired during evolutionary divergence of Acinetobacter calcoaceticus chromosomal benABC and Pseudomonas putida TOL pWW0 plasmid xylXYZ, genes encoding benzoate dioxygenases

scientific article (publication date: December 1991)

Predominant growth of Alcanivorax strains in oil-contaminated and nutrient-supplemented sea water

scientific article

Primary structure of xylene monooxygenase: similarities to and differences from the alkane hydroxylation system

scientific article (publication date: March 1991)

Proton transfer in benzyl alcohol dehydrogenase during catalysis: alternate proton-relay routes.

scientific article

Pseudovibrio ascidiaceicola sp. nov., isolated from ascidians (sea squirts).

scientific article

Purification and characterisation of the NADH:acceptor reductase component of xylene monooxygenase encoded by the TOL plasmid pWW0 of Pseudomonas putida mt-2

scientific article published on October 1, 1992

Purification and characterization of Acinetobacter calcoaceticus 4-hydroxybenzoate 3-hydroxylase after its overexpression in Escherichia coli.

scientific article published in June 1995

Reclamation of an activated-sludge microbial consortium by selective biostimulation

scientific article

Relation of Chemotactic Response to the Amount of Receptor: Evidence for Different Efficiencies of Signal Transduction ⬇️

scientific article published on June 1, 1979

Relationships between colony morphotypes and oil tolerance in Rhodococcus rhodochrous

scientific article

Retrieval of entire genes from environmental DNA by inverse PCR with pre-amplification of target genes using primers containing locked nucleic acids.

scientific article published on 17 December 2007

Rhodanobacter sp. strain BPC1 in a benzo[a]pyrene-mineralizing bacterial consortium

scientific article

Sandarakinotalea sediminis gen. nov., sp. nov., a novel member of the family Flavobacteriaceae.

scientific article

Sediminicola luteus gen. nov., sp. nov., a novel member of the family Flavobacteriaceae.

scientific article

Sediminitomix flava gen. nov., sp. nov., of the phylum Bacteroidetes, isolated from marine sediment.

scientific article

Sensory transduction in bacterial chemotaxis.

scientific article published on January 1983

Sequential enrichment of microbial populations exhibiting enhanced biodegradation of crude oil.

scientific article published in September 1995

Serinibacter salmoneus gen. nov., sp. nov., an actinobacterium isolated from the intestinal tract of a fish, and emended descriptions of the families Beutenbergiaceae and Bogoriellaceae

scientific article published on 23 July 2009

Significantly enhanced stability of glucose dehydrogenase by directed evolution.

scientific article

Single-stranded DNA family shuffling

scientific article

Stable augmentation of activated sludge with foreign catabolic genes harboured by an indigenous dominant bacterium

scientific article published in October 2002

Starvation improves survival of bacteria introduced into activated sludge.

scientific article

Structural analysis of an acidic, fatty acid ester-bonded extracellular polysaccharide produced by a pristane-assimilating marine bacterium, Rhodococcus erythropolis PR4.

scientific article

Structural analysis of an extracellular polysaccharide produced by Rhodococcus rhodochrous strain S-2

scientific article published on 01 May 2006

Structural analysis of mucoidan, an acidic extracellular polysaccharide produced by a pristane-assimilating marine bacterium, Rhodococcus erythropolis PR4.

scientific article

Structure of the Trg protein: Homologies with and differences from other sensory transducers of Escherichia coli ⬇️

scientific article published on June 1, 1984

Structure of the ring cleavage product of 1-hydroxy-2-naphthoate, an intermediate of the phenanthrene-degradative pathway of Nocardioides sp. strain KP7

scientific article

Studies on the export of the maltose-binding protein and the LamB protein

scientific article published on 01 January 1982

Substrate specificity differences between two catechol 2,3-dioxygenases encoded by the TOL and NAH plasmids from Pseudomonas putida.

scientific article

Substrate specificity of catechol 2,3-dioxygenase encoded by TOL plasmid pWW0 of Pseudomonas putida and its relationship to cell growth

scientific article

Substrate-specificity of benzyl alcohol dehydrogenase and benzaldehyde dehydrogenase encoded by TOL plasmid pWW0. Metabolic and mechanistic implications.

scientific article published on May 1992

Synthesis of L-threo-3,4-dihydroxyphenylserine (L-threo-DOPS) with thermostabilized low-specific L-threonine aldolase from Streptomyces coelicolor A3(2).

scientific article published in May 2007

Taxonomic distribution of Streptomyces species capable of producing bioactive compounds among strains preserved at NITE/NBRC.

scientific article published on 2 July 2008

The E. coli K-12 chromosome flanked by two IS10 sequences transposes

scientific article published on 01 January 1984

The TOL plasmid pWW0 xylN gene product from Pseudomonas putida is involved in m-xylene uptake.

scientific article

The bacterivorous soil flagellate Heteromita globosa reduces bacterial clogging under denitrifying conditions in sand-filled aquifer columns.

scientific article published on September 2002

The gene pvaB encodes oxidized polyvinyl alcohol hydrolase of Pseudomonas sp. strain VM15C and forms an operon with the polyvinyl alcohol dehydrogenase gene pvaA.

scientific article published in March 2000

The meta cleavage operon of TOL degradative plasmid pWW0 comprises 13 genes.

scientific article

The potential of Cycloclasticus and Altererythrobacter strains for use in bioremediation of petroleum-aromatic-contaminated tropical marine environments

scientific article

The soil flagellate Heteromita globosa accelerates bacterial degradation of alkylbenzenes through grazing and acetate excretion in batch culture

scientific article published on 24 January 2005

Tn1 insertion mutagenesis in Escherichia coli K-12 using a temperature-sensitive mutant of plasmid RP4

scientific article published on 01 January 1981

Tn501 insertion mutagenesis in Pseudomonas aeruginosa PAO

scientific article published on 01 January 1984

Transposon mutagenesis analysis of meta-cleavage pathway operon genes of the TOL plasmid of Pseudomonas putida mt-2 ⬇️

scientific article published on October 1, 1984

Transposon-insertion mutants of Escherichia coli K12 defective in a component common to galactose and ribose chemotaxis ⬇️

scientific article published on March 20, 1979

Two groups of thermophilic amino acid aminotransferases exhibiting broad substrate specificities for the synthesis of phenylglycine derivatives

scientific article published on 15 May 2008

Understanding the diversity in catabolic potential of microorganisms for the development of bioremediation strategies.

scientific article

[SWISS-PROT: the curated protein sequence database on Internet]

scientific article published on 01 January 2001

cis-diol dehydrogenases encoded by the TOL pWW0 plasmid xylL gene and the Acinetobacter calcoaceticus chromosomal benD gene are members of the short-chain alcohol dehydrogenase superfamily

scientific article

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