List of works - Lee R Lynd

13 Gene Transfer Systems for Obligately Anaerobic Thermophilic Bacteria

article

A Comparison between Hot Liquid Water and Steam Fractionation of Corn Fiber

article published in 2001

A comparison of liquid hot water and steam pretreatments of sugar cane bagasse for bioconversion to ethanol ⬇️

scientific article published on January 1, 2002

A defined growth medium with very low background carbon for culturing Clostridium thermocellum

scientific article published on 14 February 2012

A functionally based model for hydrolysis of cellulose by fungal cellulase.

scientific article

A global conversation about energy from biomass: the continental conventions of the global sustainable bioenergy project

scientific article

A kinetic model for simultaneous saccharification and fermentation of Avicel with Saccharomyces cerevisiae

scientific article published on 30 November 2010

A markerless gene deletion and integration system for Thermoanaerobacter ethanolicus

scientific article published on 4 May 2016

A mutation in the AdhE alcohol dehydrogenase of Clostridium thermocellum increases tolerance to several primary alcohols, including isobutanol, n-butanol and ethanol

scientific article published in Scientific Reports

A transition from cellulose swelling to cellulose dissolution by o-phosphoric acid: evidence from enzymatic hydrolysis and supramolecular structure

scientific article

Allocation of ATP to synthesis of cells and hydrolytic enzymes in cellulolytic fermentative microorganisms: bioenergetics, kinetics, and bioprocessing

scientific article published on 01 April 1998

Assessment of yield gaps on global grazed-only permanent pasture using climate binning

scientific article published on 01 January 2020

Bioenergetics and end-product regulation of Clostridium thermosaccharolyticum in response to nutrient limitation.

scientific article published in September 1993

Bioenergy crop models: descriptions, data requirements, and future challenges

article by Sujithkumar Surendran Nair et al published 13 March 2012 in GCB Bioenergy

Biosynthesis of radiolabeled cellodextrins by the Clostridium thermocellum cellobiose and cellodextrin phosphorylases for measurement of intracellular sugars.

scientific article

Both adhE and a separate NADPH-dependent alcohol dehydrogenase (adhA) are necessary for high ethanol production in Thermoanaerobacterium saccharolyticum.

scientific article published on 14 November 2016

Cellodextrin preparation by mixed-acid hydrolysis and chromatographic separation

scientific article published in November 2003

Cellulose degradation and ethanol production by thermophilic bacteria using mineral growth medium.

scientific article published in January 1996

Cellulose utilization by Clostridium thermocellum: bioenergetics and hydrolysis product assimilation.

scientific article

Cellulose- and Xylan-Degrading Thermophilic Anaerobic Bacteria from Biocompost ⬇️

scientific article published on February 11, 2011

Cellulosic ethanol: status and innovation.

scientific article

Characterization of 13 newly isolated strains of anaerobic, cellulolytic, thermophilic bacteria ⬇️

scientific article published on November 1, 2001

Characterization of Clostridium thermocellum strains with disrupted fermentation end-product pathways

scientific article published on 5 May 2013

Characterization of the Clostridium thermocellum AdhE, NfnAB, ferredoxin and Pfor proteins for their ability to support high titer ethanol production in Thermoanaerobacterium saccharolyticum

scientific article published on 12 September 2018

Cloning and expression of the Clostridium thermocellum L-lactate dehydrogenase gene in Escherichia coli and enzyme characterization

scientific article

Closing the carbon balance for fermentation by Clostridium thermocellum (ATCC 27405).

scientific article published on 8 October 2011

Clostridium thermocellum releases coumaric acid during degradation of untreated grasses by the action of an unknown enzyme

scientific article published on 14 January 2016

Coculture of Staphylococcus aureus with Pseudomonas aeruginosa Drives S. aureus towards Fermentative Metabolism and Reduced Viability in a Cystic Fibrosis Model

scientific article published on 27 April 2015

Comparative efficiency and driving range of light- and heavy-duty vehicles powered with biomass energy stored in liquid fuels or batteries

scientific article

Complete Genome Sequence of Clostridium clariflavum DSM 19732.

scientific article

Computational design and characterization of a temperature-sensitive plasmid replicon for gram positive thermophiles ⬇️

scientific article published on May 11, 2012

Consolidated bioprocessing for bioethanol production using Saccharomyces cerevisiae

scientific article published on January 2007

Consolidated bioprocessing of cellulosic biomass: an update

scientific article (publication date: October 2005)

Conversion for Avicel and AFEX pretreated corn stover by Clostridium thermocellum and simultaneous saccharification and fermentation: insights into microbial conversion of pretreated cellulosic biomass

scientific article published on 14 May 2011

Conversion of paper sludge to ethanol in a semicontinuous solids-fed reactor ⬇️

scientific article published on November 13, 2003

Conversion of paper sludge to ethanol, II: process design and economic analysis.

scientific article

Conversion of paper sludge to ethanol. I: impact of feeding frequency and mixing energy characterization

scientific article

Conversion of phosphoenolpyruvate to pyruvate in Thermoanaerobacterium saccharolyticum

scientific article published on 23 January 2020

Correction for Lo et al., "The Bifunctional Alcohol and Aldehyde Dehydrogenase Gene, , Is Necessary for Ethanol Production in Clostridium thermocellum and Thermoanaerobacterium saccharolyticum"

article

Dcm methylation is detrimental to plasmid transformation in Clostridium thermocellum.

scientific article published on 6 May 2012

Deletion of the Cel48S cellulase from Clostridium thermocellum.

scientific article

Deletion of the hfsB gene increases ethanol production in Thermoanaerobacterium saccharolyticum and several other thermophilic anaerobic bacteria

scientific article published on 30 November 2017

Determination of the number-average degree of polymerization of cellodextrins and cellulose with application to enzymatic hydrolysis

scientific article published in May 2005

Determining the roles of the three alcohol dehydrogenases (AdhA, AdhB and AdhE) in Thermoanaerobacter ethanolicus during ethanol formation.

scientific article published on 11 January 2017

Development and characterization of stable anaerobic thermophilic methanogenic microbiomes fermenting switchgrass at decreasing residence times

article

Development and evaluation of methods to infer biosynthesis and substrate consumption in cultures of cellulolytic microorganisms

scientific article published on 30 April 2013

Development of a core Clostridium thermocellum kinetic metabolic model consistent with multiple genetic perturbations

scientific article

Development of a plasmid-based expression system in Clostridium thermocellum and its use to screen heterologous expression of bifunctional alcohol dehydrogenases (adhEs).

scientific article published on 22 April 2016

Development of both type I-B and type II CRISPR/Cas genome editing systems in the cellulolytic bacterium Clostridium thermocellum

scientific article published on 28 November 2019

Development of pyrF-based genetic system for targeted gene deletion in Clostridium thermocellum and creation of a pta mutant

scientific article

Diversity of bacteria and glycosyl hydrolase family 48 genes in cellulolytic consortia enriched from thermophilic biocompost

scientific article

Draft Genome Sequence of the Cellulolytic and Xylanolytic Thermophile Clostridium clariflavum Strain 4-2a

scientific article published on 23 July 2015

Electrotransformation of Clostridium thermocellum.

scientific article

Electrotransformation ofClostridium thermosaccharolyticum

scientific article published on 01 June 1996

Elimination of hydrogenase active site assembly blocks H2 production and increases ethanol yield in Clostridium thermocellum

scientific article

Energy. Beneficial biofuels--the food, energy, and environment trilemma

scientific article

Engineering electron metabolism to increase ethanol production in Clostridium thermocellum.

scientific article published on 27 October 2016

Enhanced Microbial Utilization of Recalcitrant Cellulose by an Ex Vivo Cellulosome-Microbe Complex ⬇️

scientific article published on December 30, 2011

Enhanced ethanol formation by Clostridium thermocellum via pyruvate decarboxylase.

scientific article published on 4 October 2017

Enzyme inactivation by ethanol and development of a kinetic model for thermophilic simultaneous saccharification and fermentation at 50 °C with Thermoanaerobacterium saccharolyticum ALK2 ⬇️

scientific article published on February 18, 2011

Enzyme-microbe synergy during cellulose hydrolysis by Clostridium thermocellum

scholarly article

Ethanol and anaerobic conditions reversibly inhibit commercial cellulase activity in thermophilic simultaneous saccharification and fermentation (tSSF)

scientific article

Ethanol production by engineered thermophiles

scientific article

Exchange of type II dockerin-containing subunits of the Clostridium thermocellum cellulosome as revealed by SNAP-tags

scientific article published on 30 November 2012

Expressing the in engineered improves ethanol production

scientific article published on 06 September 2018

Expression of adhA from different organisms in Clostridium thermocellum

scientific article published on 30 November 2017

Ferredoxin:NAD+ Oxidoreductase of Thermoanaerobacterium saccharolyticum and Its Role in Ethanol Formation.

scientific article published on 30 September 2016

Form and function of Clostridium thermocellum biofilms.

scientific article published on 19 October 2012

Formation and characterization of non-growth states in Clostridium thermocellum: spores and L-forms

scientific article

Fractionating recalcitrant lignocellulose at modest reaction conditions

scientific article

Fuel ethanol from cellulosic biomass.

scientific article

Functional heterologous expression of an engineered full length CipA from Clostridium thermocellum in Thermoanaerobacterium saccharolyticum.

scientific article published on March 2013

Genome Sequences of Industrially Relevant Saccharomyces cerevisiae Strain M3707, Isolated from a Sample of Distillers Yeast and Four Haploid Derivatives

scientific article published on 27 June 2013

Glycolysis without pyruvate kinase in Clostridium thermocellum.

scientific article

High-value renewable energy from prairie grasses

scientific article published in May 2002

Hydrolysis and fermentation of amorphous cellulose by recombinant Saccharomyces cerevisiae

scientific article published on 16 September 2006

Hydrolysis of dilute acid pretreated mixed hardwood and purified microcrystalline cellulose by cell-free broth from Clostridium thermocellum.

scientific article published in January 1987

Identification of the [FeFe]-hydrogenase responsible for hydrogen generation in Thermoanaerobacterium saccharolyticum and demonstration of increased ethanol yield via hydrogenase knockout.

scientific article published on 31 July 2009

Integrated analysis of hydrothermal flow through pretreatment.

scientific article

Interdisciplinary core curriculum based on engineering systems

Kinetic modeling of cellulosic biomass to ethanol via simultaneous saccharification and fermentation: Part II. Experimental validation using waste paper sludge and anticipation of CFD analysis.

scientific article

Kinetic modeling of xylan hydrolysis in co- and countercurrent liquid hot water flow-through pretreatments

scientific article published on 11 December 2012

Kinetics and relative importance of phosphorolytic and hydrolytic cleavage of cellodextrins and cellobiose in cell extracts of Clostridium thermocellum

scientific article published on March 2004

Kinetics of cellobiose hydrolysis using cellobiase composites from Ttrichoderma reesei and Aspergillus niger

scientific article published on 01 April 1985

Lee Lynd ⬇️

scientific article published on March 1, 2011

Lignocellulose deconstruction in the biosphere

scientific article published on 31 October 2017

Make way for ethanol.

scientific article published on November 2010

Metabolic engineering of Clostridium thermocellum for n-butanol production from cellulose

scientific article published on 23 July 2019

Metabolic engineering of Thermoanaerobacterium saccharolyticum for n-butanol production

scientific article published on 9 November 2013

Metabolic engineering of a thermophilic bacterium to produce ethanol at high yield.

scholarly article

Metabolome analysis reveals a role for glyceraldehyde 3-phosphate dehydrogenase in the inhibition of C. thermocellum by ethanol

scientific article published on 30 November 2017

Microbial cellulose utilization: fundamentals and biotechnology

scientific article

Multiple levers for overcoming the recalcitrance of lignocellulosic biomass

scientific article published on 17 January 2019

Mutant alcohol dehydrogenase leads to improved ethanol tolerance in Clostridium thermocellum

scientific article (publication date: 16 August 2011)

Mutant selection and phenotypic and genetic characterization of ethanol-tolerant strains of Clostridium thermocellum

scientific article published on 27 August 2011

Natural competence in Thermoanaerobacter and Thermoanaerobacterium species.

scientific article published on 14 May 2010

Nicotinamide cofactor ratios in engineered strains of Clostridium thermocellum and Thermoanaerobacterium saccharolyticum

scientific article published on 11 April 2016

OPTIMIZATION OF AFFINITY DIGESTION FOR THE ISOLATION OF CELLULOSOMES FROMClostridium thermocellum

scientific article published on 01 January 2014

Physiological roles of pyruvate ferredoxin oxidoreductase and pyruvate formate-lyase in Thermoanaerobacterium saccharolyticum JW/SL-YS485

scientific article published on 15 September 2015

Profile of secreted hydrolases, associated proteins, and SlpA in Thermoanaerobacterium saccharolyticum during the degradation of hemicellulose.

scientific article

Progress in understanding and overcoming biomass recalcitrance: a BioEnergy Science Center (BESC) perspective.

scientific article published on 30 November 2017

Promiscuous plasmid replication in thermophiles: Use of a novel hyperthermophilic replicon for genetic manipulation of Clostridium thermocellum at its optimum growth temperature.

scientific article published on 29 January 2016

Quantification of Cell and Cellulase Mass Concentrations during Anaerobic Cellulose Fermentation: Development of an Enzyme-Linked Immunosorbent Assay-Based Method with Application toClostridiumthermocellumBatch Cultures

article

Quantitative determination of cellulase concentration as distinct from cell concentration in studies of microbial cellulose utilization: analytical framework and methodological approach.

scientific article published in February 2002

Reactor scale up for biological conversion of cellulosic biomass to ethanol

scientific article published on 2 August 2009

Recent progress in consolidated bioprocessing

scientific article published on 14 December 2011

Redirecting carbon flux through exogenous pyruvate kinase to achieve high ethanol yields in Clostridium thermocellum

scientific article published on 29 November 2012

Regulation of cellulase synthesis in batch and continuous cultures of Clostridium thermocellum

scientific article

Rheological properties of corn stover slurries during fermentation by

scientific article published on 08 September 2018

Robust paths to net greenhouse gas mitigation and negative emissions via advanced biofuels

scientific article published on 24 August 2020

Salt accumulation resulting from base added for pH control, and not ethanol, limits growth of Thermoanaerobacteriumthermosaccharolyticum HG-8 at elevated feed xylose concentrations in continuous culture.

scientific article

Sequencing of multiple clostridial genomes related to biomass conversion and biofuel production

scientific article

Simultaneous achievement of high ethanol yield and titer in Clostridium thermocellum.

scientific article published on 02 June 2016

Simultaneous saccharification and co-fermentation of paper sludge to ethanol by Saccharomyces cerevisiae RWB222--Part I: kinetic modeling and parameters.

scientific article

Take a closer look: biofuels can support environmental, economic and social goals.

scientific article

Technoeconomic and life-cycle analysis of single-step catalytic conversion of wet ethanol into fungible fuel blendstocks

scientific article published on 25 November 2019

Testing alternative kinetic models for utilization of crystalline cellulose (Avicel) by batch cultures of Clostridium thermocellum ⬇️

scientific article published on April 29, 2013

The ethanol pathway from Thermoanaerobacterium saccharolyticum improves ethanol production in Clostridium thermocellum

scientific article

The grand challenge of cellulosic biofuels.

scientific article published in October 2017

The identification of four histidine kinases that influence sporulation in Clostridium thermocellum.

scientific article published on 14 June 2014

The pentose phosphate pathway of cellulolytic clostridia relies on 6-phosphofructokinase instead of transaldolase

scientific article published on 22 December 2019

The redox-sensing protein Rex modulates ethanol production in Thermoanaerobacterium saccharolyticum.

scientific article

Theoretical analysis of selection-based strain improvement for microorganisms with growth dependent upon extracytoplasmic enzymes.

scientific article published in October 2005

Thermodynamic analysis of the pathway for ethanol production from cellobiose in Clostridium thermocellum

scientific article published on 17 June 2019

Toward an aggregated understanding of enzymatic hydrolysis of cellulose: noncomplexed cellulase systems.

scientific article

Transformation of Clostridium Thermocellum by Electroporation

scientific article published on 01 January 2012

Voices of biotech

scientific article published on March 2016

List of works by Lee R Lynd

13 Gene Transfer Systems for Obligately Anaerobic Thermophilic Bacteria

A Comparison between Hot Liquid Water and Steam Fractionation of Corn Fiber

A comparison of liquid hot water and steam pretreatments of sugar cane bagasse for bioconversion to ethanol ⬇️

A defined growth medium with very low background carbon for culturing Clostridium thermocellum

A functionally based model for hydrolysis of cellulose by fungal cellulase.

A global conversation about energy from biomass: the continental conventions of the global sustainable bioenergy project

A kinetic model for simultaneous saccharification and fermentation of Avicel with Saccharomyces cerevisiae

A markerless gene deletion and integration system for Thermoanaerobacter ethanolicus

A mutation in the AdhE alcohol dehydrogenase of Clostridium thermocellum increases tolerance to several primary alcohols, including isobutanol, n-butanol and ethanol

A transition from cellulose swelling to cellulose dissolution by o-phosphoric acid: evidence from enzymatic hydrolysis and supramolecular structure

Allocation of ATP to synthesis of cells and hydrolytic enzymes in cellulolytic fermentative microorganisms: bioenergetics, kinetics, and bioprocessing

Assessment of yield gaps on global grazed-only permanent pasture using climate binning

Bioenergetics and end-product regulation of Clostridium thermosaccharolyticum in response to nutrient limitation.

Bioenergy crop models: descriptions, data requirements, and future challenges

Biosynthesis of radiolabeled cellodextrins by the Clostridium thermocellum cellobiose and cellodextrin phosphorylases for measurement of intracellular sugars.

Both adhE and a separate NADPH-dependent alcohol dehydrogenase (adhA) are necessary for high ethanol production in Thermoanaerobacterium saccharolyticum.

Cellodextrin preparation by mixed-acid hydrolysis and chromatographic separation

Cellulose degradation and ethanol production by thermophilic bacteria using mineral growth medium.

Cellulose utilization by Clostridium thermocellum: bioenergetics and hydrolysis product assimilation.

Cellulose- and Xylan-Degrading Thermophilic Anaerobic Bacteria from Biocompost ⬇️

Cellulosic ethanol: status and innovation.

Characterization of 13 newly isolated strains of anaerobic, cellulolytic, thermophilic bacteria ⬇️

Characterization of Clostridium thermocellum strains with disrupted fermentation end-product pathways

Characterization of the Clostridium thermocellum AdhE, NfnAB, ferredoxin and Pfor proteins for their ability to support high titer ethanol production in Thermoanaerobacterium saccharolyticum

Cloning and expression of the Clostridium thermocellum L-lactate dehydrogenase gene in Escherichia coli and enzyme characterization

Closing the carbon balance for fermentation by Clostridium thermocellum (ATCC 27405).

Clostridium thermocellum releases coumaric acid during degradation of untreated grasses by the action of an unknown enzyme

Coculture of Staphylococcus aureus with Pseudomonas aeruginosa Drives S. aureus towards Fermentative Metabolism and Reduced Viability in a Cystic Fibrosis Model

Comparative efficiency and driving range of light- and heavy-duty vehicles powered with biomass energy stored in liquid fuels or batteries

Complete Genome Sequence of Clostridium clariflavum DSM 19732.

Computational design and characterization of a temperature-sensitive plasmid replicon for gram positive thermophiles ⬇️

Consolidated bioprocessing for bioethanol production using Saccharomyces cerevisiae

Consolidated bioprocessing of cellulosic biomass: an update

Conversion for Avicel and AFEX pretreated corn stover by Clostridium thermocellum and simultaneous saccharification and fermentation: insights into microbial conversion of pretreated cellulosic biomass

Conversion of paper sludge to ethanol in a semicontinuous solids-fed reactor ⬇️

Conversion of paper sludge to ethanol, II: process design and economic analysis.

Conversion of paper sludge to ethanol. I: impact of feeding frequency and mixing energy characterization

Conversion of phosphoenolpyruvate to pyruvate in Thermoanaerobacterium saccharolyticum

Correction for Lo et al., "The Bifunctional Alcohol and Aldehyde Dehydrogenase Gene, , Is Necessary for Ethanol Production in Clostridium thermocellum and Thermoanaerobacterium saccharolyticum"

Dcm methylation is detrimental to plasmid transformation in Clostridium thermocellum.

Deletion of the Cel48S cellulase from Clostridium thermocellum.

Deletion of the hfsB gene increases ethanol production in Thermoanaerobacterium saccharolyticum and several other thermophilic anaerobic bacteria

Determination of the number-average degree of polymerization of cellodextrins and cellulose with application to enzymatic hydrolysis

Determining the roles of the three alcohol dehydrogenases (AdhA, AdhB and AdhE) in Thermoanaerobacter ethanolicus during ethanol formation.

Development and characterization of stable anaerobic thermophilic methanogenic microbiomes fermenting switchgrass at decreasing residence times

Development and evaluation of methods to infer biosynthesis and substrate consumption in cultures of cellulolytic microorganisms

Development of a core Clostridium thermocellum kinetic metabolic model consistent with multiple genetic perturbations

Development of a plasmid-based expression system in Clostridium thermocellum and its use to screen heterologous expression of bifunctional alcohol dehydrogenases (adhEs).

Development of both type I-B and type II CRISPR/Cas genome editing systems in the cellulolytic bacterium Clostridium thermocellum

Development of pyrF-based genetic system for targeted gene deletion in Clostridium thermocellum and creation of a pta mutant

Diversity of bacteria and glycosyl hydrolase family 48 genes in cellulolytic consortia enriched from thermophilic biocompost

Draft Genome Sequence of the Cellulolytic and Xylanolytic Thermophile Clostridium clariflavum Strain 4-2a

Electrotransformation of Clostridium thermocellum.

Electrotransformation ofClostridium thermosaccharolyticum

Elimination of hydrogenase active site assembly blocks H2 production and increases ethanol yield in Clostridium thermocellum

Energy. Beneficial biofuels--the food, energy, and environment trilemma

Engineering electron metabolism to increase ethanol production in Clostridium thermocellum.

Enhanced Microbial Utilization of Recalcitrant Cellulose by an Ex Vivo Cellulosome-Microbe Complex ⬇️

Enhanced ethanol formation by Clostridium thermocellum via pyruvate decarboxylase.

Enzyme inactivation by ethanol and development of a kinetic model for thermophilic simultaneous saccharification and fermentation at 50 °C with Thermoanaerobacterium saccharolyticum ALK2 ⬇️

Enzyme-microbe synergy during cellulose hydrolysis by Clostridium thermocellum

Ethanol and anaerobic conditions reversibly inhibit commercial cellulase activity in thermophilic simultaneous saccharification and fermentation (tSSF)

Ethanol production by engineered thermophiles

Exchange of type II dockerin-containing subunits of the Clostridium thermocellum cellulosome as revealed by SNAP-tags

Expressing the in engineered improves ethanol production

Expression of adhA from different organisms in Clostridium thermocellum

Ferredoxin:NAD+ Oxidoreductase of Thermoanaerobacterium saccharolyticum and Its Role in Ethanol Formation.

Form and function of Clostridium thermocellum biofilms.

Formation and characterization of non-growth states in Clostridium thermocellum: spores and L-forms

Fractionating recalcitrant lignocellulose at modest reaction conditions

Fuel ethanol from cellulosic biomass.

Functional heterologous expression of an engineered full length CipA from Clostridium thermocellum in Thermoanaerobacterium saccharolyticum.

Genome Sequences of Industrially Relevant Saccharomyces cerevisiae Strain M3707, Isolated from a Sample of Distillers Yeast and Four Haploid Derivatives

Glycolysis without pyruvate kinase in Clostridium thermocellum.

High-value renewable energy from prairie grasses

Hydrolysis and fermentation of amorphous cellulose by recombinant Saccharomyces cerevisiae

Hydrolysis of dilute acid pretreated mixed hardwood and purified microcrystalline cellulose by cell-free broth from Clostridium thermocellum.

Identification of the [FeFe]-hydrogenase responsible for hydrogen generation in Thermoanaerobacterium saccharolyticum and demonstration of increased ethanol yield via hydrogenase knockout.

Integrated analysis of hydrothermal flow through pretreatment.