Search filters

List of works by Teruyuki Nagamune

A Chemically Inducible Helper Module for Detecting Protein-Protein Interactions with Tunable Sensitivity Based on KIPPIS.

scientific article published on 6 April 2017

A bromocoumarin-based linker for synthesis of photocleavable peptidoconjugates with high photosensitivity

scientific article published on 19 September 2008

A caged phosphopeptide-based approach for photochemical activation of kinases in living cells.

scientific article published on July 2008

A general method to select antibody fragments suitable for noncompetitive detection of monovalent antigens.

scientific article

A growth signal with an artificially induced erythropoietin receptor-gp130 cytoplasmic domain heterodimer.

scientific article

A homogeneous and noncompetitive immunoassay based on the enhanced fluorescence resonance energy transfer by leucine zipper interaction

scientific article published in November 2002

A homogeneous noncompetitive immunoassay for the detection of small haptens

scientific article

A new method of ATP regeneration rate measurement using a pH meter

scientific article published on 01 January 1999

A novel platform for antibody library selection in mammalian cells based on a growth signalobody.

scientific article

A novel protein-based heme sensor consisting of green fluorescent protein and apocytochrome b562

scientific article published on 01 June 2003

AAG8 promotes carcinogenesis by activating STAT3.

scientific article published on 12 April 2014

AMEGA: antigen-mediated genetically modified cell amplification.

scientific article published on January 2004

Amino Acid Residues of G-Protein-Coupled Receptors Critical for Endoplasmic Reticulum Export and Trafficking

scientific article published on 01 January 2013

Amino acid residues critical for endoplasmic reticulum export and trafficking of platelet-activating factor receptor

scientific article

An epitope-directed antibody affinity maturation system utilizing mammalian cell survival as readout

scientific article published on 29 March 2019

An optimized homogeneous noncompetitive immunoassay based on the antigen-driven enzymatic complementation.

scientific article published in August 2003

Analysis of cellular phenotype during in vitro immunization of murine splenocytes for generating antigen-specific immunoglobulin

scientific article published on 22 November 2012

Antigen-responsive regulation of Cell motility and migration via the signalobodies based on c-Fms and c-Mpl

scientific article published on 13 January 2014

Artificial chaperone-assisted refolding in a microchannel

Artificial self-sufficient P450 in reversed micelles

scientific article published on 27 April 2010

Avidin-biotin-based approach to forming heterotypic cell clusters and cell sheets on a gas-permeable membrane

scientific article published on 11 August 2011

Biomolecular engineering for nanobio/bionanotechnology

scientific article published on 24 April 2017

Bioseparation Engineering. Evaluation of Surface Hydrophobicity of Proteins on Solid Phase Using Surface Plasmon Resonance Sensor

Bone regeneration using collagen type I vitrigel with bone morphogenetic protein-2.

scientific article published in March 2009

Ca2+ -independent sortase-A exhibits high selective protein ligation activity in the cytoplasm of Escherichia coli.

scientific article published on 28 April 2015

Casual cell surface remodeling using biocompatible lipid-poly(ethylene glycol)(n): development of stealth cells and monitoring of cell membrane behavior in serum-supplemented conditions.

scientific article published in August 2004

Cell adhesion ability of artificial extracellular matrix proteins containing a long repetitive Arg-Gly-Asp sequence.

scientific article published on July 2005

Cell fate conversion by conditionally switching the signal-transducing domain of signalobodies

scientific article published on 9 August 2013

Cell transfer printing from patterned poly(ethylene glycol)-oleyl surfaces to biological hydrogels for rapid and efficient cell micropatterning

scientific article

Cell-Based Odorant Sensor Array for Odor Discrimination Based on Insect Odorant Receptors

scientific article published on 16 July 2016

Cell-Surface Expression Levels Are Important for Fine-Tuning the Performance of Receptor Tyrosine Kinase-Based Signalobodies

scientific article published on 7 September 2017

Collagen Surfaces Modified with Photo-Cleavable Polyethylene Glycol-Lipid Support Versatile Single-Cell Arrays of Both Non-adherent and Adherent Cells

Comparison of efficiency of terminal differentiation of oligodendrocytes from induced pluripotent stem cells versus embryonic stem cells in vitro

scientific article published on 14 December 2009

Conformations of variably linked chimeric proteins evaluated by synchrotron X-ray small-angle scattering.

scientific article published in December 2004

Construction of a small-molecule-integrated semisynthetic split intein for in vivo protein ligation

scientific article published on 10 October 2007

Construction of green fluorescent protein reporter genes for genotoxicity test (SOS/umu-test) and improvement of mutagen-sensitivity

scientific article published in January 2001

Control of gene expression using caged plasmids with functionalized photo-cleavable linkers

article

Correction to Peptide Tag-Induced Horseradish Peroxidase-Mediated Preparation of a Streptavidin-Immobilized Redox-Sensitive Hydrogel

scientific article published on 21 December 2016

Correlation between Activity and Molecular Structure around the Active Center of Cytochrome P450cam Conjugates

Covalent split protein fragment-DNA hybrids generated through N-terminus-specific modification of proteins by oligonucleotides

scientific article

Crystallization of a photosensitive nitrile hydratase from Rhodococcus sp. N-771

scientific article published on 01 July 1991

Death signalobody: inducing conditional cell death in response to a specific antigen.

scientific article published on 21 April 2013

Demonstration of a homogeneous noncompetitive immunoassay based on bioluminescence resonance energy transfer.

scientific article

Design of Ca2+-independentStaphylococcus aureussortase A mutants

scientific article published on 04 July 2012

Design of an Artificial Light-Harvesting Unit by Protein Engineering: Cytochrome b562–Green Fluorescent Protein Chimera

scientific article published on 01 November 2001

Design of the linkers which effectively separate domains of a bifunctional fusion protein.

scientific article

Detecting protein-protein interactions based on kinase-mediated growth induction of mammalian cells

scientific article published on 19 August 2014

Detection of protein-protein interaction by bioluminescence resonance energy transfer from firefly luciferase to red fluorescent protein

scientific article published in January 2002

Development of Small Molecular Artificial Chaperone for Protein Refolding and Artificial Chaperone-Assisted Proteomics Technology

Development of a homogeneous competitive immunoassay for phosphorylated protein antigen based on the enhanced fluorescence resonance energy transfer technology

article

Development of small molecular additives for protein refolding and proteomics technology

article

Differentiation signalobody: Demonstration of antigen-dependent osteoclast differentiation from a progenitor cell line.

scientific article published on 12 March 2016

Dissection of Signaling Events Downstream of the c-Mpl Receptor in Murine Hematopoietic Stem Cells Via Motif-Engineered Chimeric Receptors.

scientific article

Domain structure of growth signalobodies critically affects the outcome of antibody library selection

scientific article published on 22 January 2015

Dynamic photochemical lipid micropatterning for manipulation of nonadherent mammalian cells

scientific article published on January 2014

Editorial: Asia Pacific Biochemical engineering

article

Effect of beta-cyclodextrin on the renaturation of enzymes after sodium dodecyl sulfate-polyacrylamide gel electrophoresis

scientific article published on 13 June 2008

Effect of culture temperature shift on the cellular sugar accumulation of Chlorella vulgaris SO-26

article

Effect of length of molecular recognition moiety on enzymatic activity switching

scientific article published on 3 May 2013

Effects of culture temperature shift and light-dark time cycle on the cellular sugar accumulation of Chlorella pyrenoidosa

article

Electron donation to an archaeal cytochrome P450 is enhanced by PCNA-mediated selective complex formation with foreign redox proteins.

scientific article

Engineering a growth sensor to select intracellular antibodies in the cytosol of mammalian cells

scientific article

Engineering of Functional Chimeric Protein G–VargulaLuciferase ⬇️

scientific article published on July 1, 1997

Enhanced fluorescence resonance energy transfer immunoassay with improved sensitivity based on the Fab'-based immunoconjugates

scientific article published on 7 November 2006

Enhancement of sortase A-mediated protein ligation by inducing a β-hairpin structure around the ligation site

Enzymatic labeling of a single chain variable fragment of an antibody with alkaline phosphatase by microbial transglutaminase

scientific article

Evaluation of surface hydrophobicity of immobilized protein with a surface plasmon resonance sensor

scientific article

Exogenous gene expression and growth regulation of hematopoietic cells via a novel human artificial chromosome

scientific article published on 7 December 2005

Expanded-Bed Protein Refolding Using a Solid-Phase Artificial Chaperone

scientific article published on 01 January 2001

Expression of amoA mRNA in wastewater treatment processes examined by competitive RT-PCR.

scientific article published in July 2004

FGF signal regulates gastrulation cell movements and morphology through its target NRH.

scientific article published in June 2005

Fabrication of a protein film by electrospray deposition method and investigation of photochemical properties by persistent spectral hole burning

scientific article published on 01 May 2003

Fabrication of protein microarrays using the electrospray deposition (ESD) method: Application of microfluidic chips in immunoassay

article published in 2010

Fine tuning of spatial arrangement of enzymes in a PCNA-mediated multienzyme complex using a rigid poly-L-proline linker

scientific article

Fluorolabeling of antibody variable domains with green fluorescent protein variants: application to an energy transfer-based homogeneous immunoassay.

scientific article published on May 2000

Fusion protein bilayer fabrication composed of recombinant azurin/cytochrome P450 by the sortase-mediated ligation method

scientific article published on 21 May 2014

Glycosylation of recombinant proforms of major house dust mite allergens Der p 1 and Der f 1 decelerates the speed of maturation

scientific article published on 26 January 2006

Growth control of genetically modified cells using an antibody/c-Kit chimera.

scientific article published on 9 January 2012

Growth promotion of genetically modified hematopoietic progenitors using an antibody/c-Mpl chimera.

scientific article published on 22 June 2011

Growth signalobody selects functional intrabodies in the mammalian cytoplasm

scientific article published on 8 December 2015

High Speed Polymerase Chain Reaction in Constant Flow

scientific article published on 01 February 1994

High density culture of lactobacillus casei by a Cross-Flow culture method based on kinetic properties of the microorganism

article

High-efficiency DNA injection into a single human mesenchymal stem cell using a nanoneedle and atomic force microscopy.

scientific article

Homogeneous sandwich immunoassay based on the enzymatic complementation induced by single-chain Fv fragments

scientific article published in April 2004

How does heme axial ligand deletion affect the structure and the function of cytochrome b(562)?

scientific article published in June 2001

Icariin induces osteogenic differentiation in vitro in a BMP- and Runx2-dependent manner.

scientific article published on 22 February 2008

Icariin: a potential osteoinductive compound for bone tissue engineering.

scientific article

Imidazolium-based polymer hydrogels with microdomains as carriers of hydrophobic molecules

Immobilization of a Bacterial Cytochrome P450 Monooxygenase System on a Solid Support

scientific article published on 26 October 2016

Immobilized culture and transfection microarray of non-adherent cells

scientific article published on January 2011

Immunopanning selection of A2B5-positive cells increased the differentiation efficiency of induced pluripotent stem cells into oligodendrocytes

scientific article published on 04 December 2010

Improved growth response of antibody/receptor chimera attained by the engineering of transmembrane domain.

scientific article

Intracellular protein cyclization catalyzed by exogenously transduced Streptococcus pyogenes sortase A.

scientific article published on 12 April 2013

Intramolecular electron transfer in a cytochrome P450cam system with a site-specific branched structure

scientific article published on 7 September 2007

Introduction of selective intersubunit disulfide bonds into self-assembly protein scaffold to enhance an artificial multienzyme complex's activity

scientific article published on 22 February 2013

Ligand-inducible dimeric antibody for selecting antibodies against a membrane protein based on mammalian cell proliferation.

scientific article published on 19 October 2015

Light-activated gene expression from site-specific caged DNA with a biotinylated photolabile protection group

scientific article published on 20 January 2010

Light-induced oxidation of iron atoms in a photosensitive nitrile hydratase

scientific article published on 01 April 1992

Log P effect of organic solvents on a thermophilic alcohol dehydrogenase

scientific article published on 5 January 2005

Luminescent and substrate binding activities of firefly luciferase N-terminal domain

scientific article published in July 2003

Measuring adsorption of a hydrophobic probe with a surface plasmon resonance sensor to monitor conformational changes in immobilized proteins

scientific article published in July 2003

Microbial ecology of nitrifying bacteria in wastewater treatment process examined by fluorescence in situ hybridization

scientific article published in January 2000

Microbial expression of proteins containing long repetitive Arg-Gly-Asp cell adhesive motifs created by overlap elongation PCR.

scientific article

Modeling tandem AAG8-MEK inhibition in melanoma cells

scientific article published on 14 March 2014

Modulating the actions of NK cell-mediated cytotoxicity using lipid-PEG (n) and inhibitory receptor-specific antagonistic peptide conjugates.

scientific article published in July 2005

Molecular Cloning and Nucleotide Sequence of the Gene Coding Photosensitive Nitrile Hydratase

article

Molecular assembly of P450 with ferredoxin and ferredoxin reductase by fusion to PCNA

scientific article published on 01 July 2010

Molecular recognition moiety and its target biomolecule interact in switching enzyme activity

Monitoring of the refolding process for immobilized firefly luciferase with a biosensor based on surface plasmon resonance.

scientific article published in January 2001

Multiple-mutation at a potential ligand-binding region decreased allergenicity of a mite allergen Der f 2 without disrupting global structure.

scientific article

N-terminal glycine-specific protein conjugation catalyzed by microbial transglutaminase

scientific article published in April 2005

Nanoscale-engineered cytochrome p450 system with a branch structure.

scientific article

Novel selection method for engineered antibodies using the mechanism of Fv fragment stabilization in the presence of antigen.

scientific article

Object-oriented fuzzy expert system for on-line diagnosing and control of bioprocesses

scientific article

Observation of charge state and conformational change in immobilized protein using surface plasmon resonance sensor.

scientific article

Open Sandwich Enzyme-Linked Immunosorbent Assay for the Quantitation of Small Haptens

scientific article published on 01 November 2000

Open sandwich ELISA: a novel immunoassay based on the interchain interaction of antibody variable region

scientific article published in December 1996

Peptide Tag-Induced Horseradish Peroxidase-Mediated Preparation of a Streptavidin-Immobilized Redox-Sensitive Hydrogel

scientific article published on 16 May 2016

Peptidyl linkers for protein heterodimerization catalyzed by microbial transglutaminase

scientific article

Photo-Cleavable Peptide-Poly(Ethylene Glycol) Conjugate Surfaces for Light-Guided Control of Cell Adhesion

scientific article published on 08 August 2020

Photocontrollable dynamic micropatterning of non-adherent mammalian cells using a photocleavable poly(ethylene glycol) lipid

scientific article

Photolytic Protein Aggregates: Versatile Materials for Controlled Release of Active Proteins

scientific article published on 4 March 2016

Photosensitive Nitrile Hydratase from Rhodococcus sp. N-771

scientific article published on 01 November 1992

Photosensitive nitrile hydratase intrinsically possesses nitric oxide bound to the non-heme iron center: evidence by Fourier transform infrared spectroscopy

article

Photosensitizer and polycationic peptide-labeled streptavidin as a nano-carrier for light-controlled protein transduction

scientific article published on 29 April 2015

Poly(ethylene glycol)-Lipid-Conjugated Antibodies Enhance Dendritic Cell Phagocytosis of Apoptotic Cancer Cells

scientific article published on 26 April 2012

Poly[G] improved protein productivity of cell-free translation by inhibiting mRNase in wheat germ extract

article published in 1999

Properties of an alcohol dehydrogenase from the hyperthermophilic archaeon Aeropyrum pernix K1.

scientific article published in January 2004

Protein Refolding by N-Alkylpyridinium and N-Alkyl-N-methylpyrrolidinium Ionic Liquids

scientific article published in 2011

Protein cell-surface display through in situ enzymatic modification of proteins with a poly(Ethylene glycol)-lipid

scientific article published on 26 April 2013

Protein productivity of cell-free translation was improved by removing phosphatase from wheat germ extract with immunoprecipitation

Protein refolding is improved by adding nonionic polyethylene glycol monooleyl ethers with various polyethylene glycol lengths

scientific article

Purification of inactivated photoresponsive nitrile hydratase

scientific article published in April 1990

Quantitative control of intracellular signaling activity through chimeric receptors incorporating multiple identical tyrosine motifs.

scientific article published on 27 November 2013

Quantitative image cytometry for analyzing intracellular trafficking of G protein-coupled receptors on a chemical-trapping single cell array

scientific article published on 5 May 2017

Rapid and efficient induction of an endogenous cell signaling event by subcellular targeting of a synthetic ligand

scientific article published in September 2009

Rapid homogeneous immunoassay of peptides based on bioluminescence resonance energy transfer from firefly luciferase

scientific article published in January 2002

Rapid protein anchoring into the membranes of Mammalian cells using oleyl chain and poly(ethylene glycol) derivatives.

scientific article published on May 2004

Rational design of a protein-based molecular device consisting of blue fluorescent protein and zinc protoporphyrin IX incorporated into a cytochrome b562 scaffold

scientific article published in January 2004

Recombinant extracellular matrix-like proteins with repetitive elastin or collagen-like functional motifs

scientific article

Recombinant hBMP4 incorporated with non-canonical amino acid for binding to hydroxyapatite.

scientific article published on 5 May 2011

Reconstitution of a cytokine receptor scaffold utilizing multiple different tyrosine motifs.

scientific article published on 28 June 2013

Rectified photocurrent in a protein based molecular photo-diode consisting of a cytochrome b562-green fluorescent protein chimera self-assembled monolayer

scientific article published in May 2004

Replacing factor-dependency with that for lysozyme: affordable culture of IL-6-dependent hybridoma by transfecting artificial cell surface receptor.

scientific article

Reversal of antigen-dependent signaling by two mutations in antibody/receptor chimera: implication of inverse agonism in cytokine receptor superfamily.

scientific article

Ribosomal RNA supplementation highly reinforced cell-free translation activity of wheat germ

scientific article published in January 2000

S-Fms signalobody enhances myeloid cell growth and migration

scientific article published on 22 January 2014

S-peptide as a potent peptidyl linker for protein cross-linking by microbial transglutaminase from Streptomyces mobaraensis

scientific article published in March 2003

Screening of FGF target genes in Xenopus by microarray: temporal dissection of the signalling pathway using a chemical inhibitor

scientific article

Secondary structure analyses of protein films on gold surfaces by circular dichroism.

scientific article published in October 2003

Selection and growth regulation of genetically modified cells with hapten-specific antibody/receptor tyrosine kinase chimera.

scientific article

Selection of cDNA candidates that induce oligomerization of NLRP3 using a chimeric receptor approach

scientific article published on 29 January 2015

Selective expansion of genetically modified T cells using an antibody/interleukin-2 receptor chimera.

scientific article

Selective intracellular vaporisation of antibody-conjugated phase-change nano-droplets in vitro

scientific article

Single Cell Reporter Assay Using Cell Surface Displayed Vargula Luciferase

scientific article published in 2001

Single cell reporter assay using cell surface displayed Vargula luciferase

scientific article published on 01 January 2001

Single-stage autotrophic nitrogen-removal process using a composite matrix immobilizing nitrifying and sulfur-denitrifying bacteria

scientific article published on 4 February 2005

Site-specific conjugation of oligonucleotides to the C-terminus of recombinant protein by expressed protein ligation

scientific article published in May 2004

Site-specific protein modification on living cells catalyzed by Sortase

scientific article

Solid-phase artificial chaperone-assisted refolding using insoluble beta-cyclodextrin-acrylamide copolymer beads

scientific article published in December 2004

Sortase A-mediated synthesis of ligand-grafted cyclized peptides for modulating a model protein-protein interaction

scientific article published on 24 April 2015

Sortase-mediated ligation: a gift from Gram-positive bacteria to protein engineering.

scientific article published on March 2009

Spectroscopic Observation of the Intramolecular Electron Transfer in the Photoactivation Processes of Nitrile Hydratase

scientific article published on 01 March 1994

Sterically bulky caging for light-inducible protein activation

scientific article

Successful control of aggregation and folding rates during refolding of denatured lysozyme by adding N-methylimidazolium cations with various N'-substituents

scientific article

Supramolecular protein assembly supports immobilization of a cytochrome P450 monooxygenase system as water-insoluble gel.

scientific article published on 3 March 2015

Synergistic effects of detergents and organic solvents on protein refolding: control of aggregation and folding rates.

scientific article published on 19 September 2010

T cell growth control using hapten-specific antibody/interleukin-2 receptor chimera.

scientific article

The influence of domain structures on the signal transduction of chimeric receptors derived from the erythropoietin receptor.

scientific article

The lifetime evaluation of vapourised phase-change nano-droplets

scientific article published on 4 April 2016

Three proliferating cell nuclear antigen homologues from Metallosphaera sedula form a head-to-tail heterotrimer

scientific article published on 27 May 2016

Top-down motif engineering of a cytokine receptor for directing ex vivo expansion of hematopoietic stem cells

scientific article published on 23 September 2013

Transfer printing of transfected cell microarrays from poly(ethylene glycol)-oleyl surfaces onto biological hydrogels

scientific article published on 22 August 2013

Tyrosine Coupling Creates a Hyperbranched Multivalent Protein Polymer Using Horseradish Peroxidase via Bipolar Conjugation Points

scientific article published on 19 April 2016

Use of Sulfolobus solfataricus PCNA Subunit Proteins to Direct the Assembly of Multimeric Enzyme Complexes

scientific article published on 01 January 2013

Visualization of the pH-dependent dynamic distribution of G2A in living cells

scientific article (publication date: September 2014)

Paulina is supported by:

About Paulina

Help