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List of works by Tomohisa Kuzuyama

"Cation-Stitching Cascade": exquisite control of terpene cyclization in cyclooctatin biosynthesis

scientific article

1-Hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate reductase (IDS) is encoded by multicopy genes in gymnosperms Ginkgo biloba and Pinus taeda

scientific article published in September 2007

A 1-deoxy-d-xylulose 5-phosphate reductoisomerase catalyzing the formation of 2-C-methyl-d-erythritol 4-phosphate in an alternative nonmevalonate pathway for terpenoid biosynthesis ⬇️

scientific article published on August 18, 1998

A new pathway for poly(3-hydroxybutyrate) production in Escherichia coli and Corynebacterium glutamicum by functional expression of a new acetoacetyl-coenzyme A synthase.

scientific article published on 7 February 2011

A suicide enzyme catalyzes multiple reactions for biotin biosynthesis in cyanobacteria

scientific article published on 10 February 2020

Alteration of coenzyme specificity of lactate dehydrogenase from Thermus thermophilus by introducing the loop region of NADP(H)-dependent malate dehydrogenase

scientific article published on 07 September 2006

Amino-group carrier-protein-mediated secondary metabolite biosynthesis in Streptomyces

scientific article published on 26 September 2016

An Unprecedented Cyclization Mechanism in the Biosynthesis of Carbazole Alkaloids in Streptomyces

scientific article published on 13 August 2019

An Unusual Skeletal Rearrangement in the Biosynthesis of the Sesquiterpene Trichobrasilenol from Trichoderma ⬇️

scientific article published on 09 September 2019

An unusual isopentenyl diphosphate isomerase found in the mevalonate pathway gene cluster from Streptomyces sp. strain CL190

scientific article

An unusual terpene cyclization mechanism involving a carbon-carbon bond rearrangement

scientific article published on 16 February 2015

Antimicrobial N-(2-chlorobenzyl)-substituted hydroxamate is an inhibitor of 1-deoxy-D-xylulose 5-phosphate synthase

scientific article published on 01 June 2013

Antisense and chemical suppression of the nonmevalonate pathway affects ent-kaurene biosynthesis in Arabidopsis

scientific article published on 12 April 2002

Aspartate kinase involved in 4-hydroxy-3-nitrosobenzamide biosynthesis in Streptomyces murayamaensis.

scientific article published on 2 August 2016

Bacillus subtilis ypgA gene is fni, a nonessential gene encoding type 2 isopentenyl diphosphate isomerase

scientific article published in January 2004

Bioactive natural products with carbon-phosphorus bonds and their biosynthesis.

scientific article

Biosynthesis of Trehangelin in Polymorphospora rubra K07-0510: Identification of Metabolic Pathway to Angelyl-CoA.

scientific article published on 17 June 2016

Biosynthesis of the antituberculous agent caprazamycin: Identification of caprazol-3"-phosphate, an unprecedented caprazamycin-related metabolite

scientific article published on 23 May 2016

Biosynthesis of versipelostatin: identification of an enzyme-catalyzed [4+2]-cycloaddition required for macrocyclization of spirotetronate-containing polyketides

scientific article

Biosynthetic Origin of the Hydroxamic Acid Moiety of Trichostatin A: Identification of Unprecedented Enzymatic Machinery Involved in Hydroxylamine Transfer

scientific article

C-Methylation Catalyzed by Fom3, a Cobalamin-Dependent Radical S-adenosyl-l-methionine Enzyme in Fosfomycin Biosynthesis, Proceeds with Inversion of Configuration

scientific article published on 12 July 2018

Characterization of 1-deoxy-D-xylulose 5-phosphate reductoisomerase, an enzyme involved in isopentenyl diphosphate biosynthesis, and identification of its catalytic amino acid residues

scientific article published on 01 June 2000

Characterization of the fomA and fomB gene products from Streptomyces wedmorensis, which confer fosfomycin resistance on Escherichia coli.

scientific article published on March 2000

Characterization of two β-decarboxylating dehydrogenases from Sulfolobus acidocaldarius

scientific article published on 2 September 2016

Cloning and characterization of 1-deoxy-D-xylulose 5-phosphate synthase from Streptomyces sp. Strain CL190, which uses both the mevalonate and nonmevalonate pathways for isopentenyl diphosphate biosynthesis

scientific article

Cloning and characterization of 2-C-methyl-D: -erythritol 2,4-cyclodiphosphate synthase (MECS) gene from Ginkgo biloba

scientific article published on 10 March 2006

Cloning and characterization of the 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway genes of a natural-rubber producing plant, Hevea brasiliensis

scientific article published on 7 November 2008

Cloning and heterologous expression of the cyclooctatin biosynthetic gene cluster afford a diterpene cyclase and two p450 hydroxylases.

scientific article published in July 2009

Cloning of a gene cluster encoding enzymes responsible for the mevalonate pathway from a terpenoid-antibiotic-producing Streptomyces strain ⬇️

scientific article published in July 2001

Comprehensive Derivatization of Thioviridamides by Heterologous Expression

scientific article published on 11 June 2019

Contribution of the mevalonate and methylerythritol phosphate pathways to the biosynthesis of gibberellins in Arabidopsis

scientific article published on 12 September 2002

Convergent strategies in biosynthesis ⬇️

scientific article published on 06 May 2011

Crystal Structure of Fosfomycin Resistance Kinase FomA from Streptomyces wedmorensis

scientific article

Crystal Structure of the LysY·LysW Complex from Thermus thermophilus

scientific article published on 9 March 2016

Crystal structure of 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase, an enzyme in the non-mevalonate pathway of isoprenoid synthesis

scientific article (publication date: 8 August 2003)

Crystal structure of NAD-dependent malate dehydrogenase complexed with NADP(H).

scientific article published in August 2005

Crystallization and preliminary X-ray diffraction analysis of cyclolavandulyl diphosphate synthase, a new member of the cis-isoprenyl diphosphate synthase superfamily.

scientific article published on 25 September 2014

Cyclolavandulyl skeleton biosynthesis via both condensation and cyclization catalyzed by an unprecedented member of the cis-isoprenyl diphosphate synthase superfamily

scientific article published on 18 March 2014

Determinants of dual substrate specificity revealed by the crystal structure of homoisocitrate dehydrogenase from Thermus thermophilus in complex with homoisocitrate·Mg(2+)·NADH.

scientific article published on 3 September 2016

Different biosynthetic pathways to fosfomycin in Pseudomonas syringae and Streptomyces species. ⬇️

scientific article

Discovery of Unprecedented Hydrazine-Forming Machinery in Bacteria

scientific article published on 17 July 2018

Discovery of an Antibacterial Isoindolinone-Containing Tetracyclic Polyketide by Cryptic Gene Activation and Characterization of Its Biosynthetic Gene Cluster

scientific article published on 17 August 2018

Discovery of proteinaceous N-modification in lysine biosynthesis of Thermus thermophilus.

scientific article published on 20 July 2009

Diversity of the biosynthesis of the isoprene units

scientific article

Efficient synthesis of functional isoprenoids from acetoacetate through metabolic pathway-engineered Escherichia coli

scientific article published on 3 October 2008

Elicitor induced activation of the methylerythritol phosphate pathway toward phytoalexins biosynthesis in rice.

scientific article published on 17 July 2007

Enhancement of the latent 3-isopropylmalate dehydrogenase activity of promiscuous homoisocitrate dehydrogenase by directed evolution

scientific article

Fosfomycin Biosynthesis via Transient Cytidylylation of 2-Hydroxyethylphosphonate by the Bifunctional Fom1 Enzyme

scientific article published on 20 July 2017

Functional analysis of eubacterial diterpene cyclases responsible for biosynthesis of a diterpene antibiotic, terpentecin.

scientific article

Genome Mining for Sesterterpenes Using Bifunctional Terpene Synthases Reveals a Unified Intermediate of Di/Sesterterpenes

scientific article published on 04 September 2015

Genome sequence of Kitasatospora setae NBRC 14216T: an evolutionary snapshot of the family Streptomycetaceae.

scientific article

Genome-wide comprehensive analysis of transcriptional regulation by ArgR in Thermus thermophilus

scientific article published on 29 July 2014

Guanidyl modification of the 1-azabicyclo[3.1.0]hexane ring in ficellomycin essential for its biological activity

scientific article published on 25 June 2020

Hetero-oligomeric glutamate dehydrogenase from Thermus thermophilus.

scientific article published on 9 September 2010

Heterologous mevalonate production in Streptomyces lividans TK23. ⬇️

scientific article published in April 2004

Identification and biosynthesis of new acyloins from the thermophilic bacterium Thermosporothrix hazakensis SK20-1(T).

scientific article published on 28 January 2014

Identification and characterization of bacterial diterpene cyclases that synthesize the cembrane skeleton.

scientific article published on February 2013

Identification of 8-amino-2,5,7-trihydroxynaphthalene-1,4-dione, a novel intermediate in the biosynthesis of Streptomyces meroterpenoids

scientific article published on 31 July 2012

Identification of class 2 1-deoxy-D-xylulose 5-phosphate synthase and 1-deoxy-D-xylulose 5-phosphate reductoisomerase genes from Ginkgo biloba and their transcription in embryo culture with respect to ginkgolide biosynthesis

scientific article published in February 2006

Identification of the biosynthetic gene cluster for the herbicide phosphonothrixin in Saccharothrix sp. ST-888

scientific article published on 29 October 2014

Interconversion of the product specificity of type I eubacterial farnesyl diphosphate synthase and geranylgeranyl diphosphate synthase through one amino acid substitution

scientific article published in January 2003

Involvement of subdomain II in the recognition of acetyl-CoA revealed by the crystal structure of homocitrate synthase from Sulfolobus acidocaldarius

scientific article published on 08 September 2020

Involvement of the arginine repressor in lysine biosynthesis of Thermus thermophilus

scientific article published on 01 December 2006

Leader peptide-mediated transcriptional attenuation of lysine biosynthetic gene cluster in Thermus thermophilus

scientific article

Lysine Biosynthesis of Thermococcus kodakarensis with the Capacity to Function as an Ornithine Biosynthetic System.

scientific article published on 26 August 2016

Lysine and arginine biosyntheses mediated by a common carrier protein in Sulfolobus

scientific article

Mechanism of Concerted Inhibition of  2 2-type Hetero-oligomeric Aspartate Kinase from Corynebacterium glutamicum

scientific article

Mechanism of Substrate Recognition and Insight into Feedback Inhibition of Homocitrate Synthase from Thermus thermophilus

scientific article

Mechanistic insights into Diels-Alder reactions in natural product biosynthesis

scientific article

Metabolic engineering for the production of prenylated polyphenols in transgenic legume plants using bacterial and plant prenyltransferases

scientific article published on 05 August 2011

Metabolic engineering of Salmonella vaccine bacteria to boost human Vγ2Vδ2 T cell immunity.

scientific article

Methylbenzene-Containing Polyketides from a Streptomyces that Spontaneously Acquired Rifampicin Resistance: Structural Elucidation and Biosynthesis

scientific article published on 24 February 2016

Methylcobalamin-Dependent Radical SAM C-Methyltransferase Fom3 Recognizes Cytidylyl-2-hydroxyethylphosphonate and Catalyzes the Nonstereoselective C-Methylation in Fosfomycin Biosynthesis

scientific article

Mevalonate and nonmevalonate pathways for the biosynthesis of isoprene units.

scientific article

Microbial glucosylation of flavonols by Cunninghamella echinulata

scientific article published on 15 March 2010

Minimum Information about a Biosynthetic Gene cluster.

scientific article published on August 2015

New 2-(1'H-indole-3'-carbonyl)-thiazoles derived from the thermophilic bacterium Thermosporothrix hazakensis SK20-1(T).

scientific article published on 23 July 2014

New approach to multiply deuterated isoprenoids using triply engineered Escherichia coli and its potential as a tool for mechanistic enzymology

scientific article published in February 2001

New glycosylated derivatives of versipelostatin, the GRP78/Bip molecular chaperone down-regulator, from Streptomyces versipellis 4083-SVS6.

scientific article published on 26 February 2009

NovQ is a prenyltransferase capable of catalyzing the addition of a dimethylallyl group to both phenylpropanoids and flavonoids

scientific article published on 26 June 2009

Novel features of the ISC machinery revealed by characterization of Escherichia coli mutants that survive without iron-sulfur clusters

scientific article published on 12 November 2015

Novel stand-alone RAM domain protein-mediated catalytic control of anthranilate phosphoribosyltransferase in tryptophan biosynthesis in Thermus thermophilus.

scientific article published on 19 October 2016

Novel tryptophan metabolism by a potential gene cluster that is widely distributed among actinomycetes. ⬇️

scientific article published on 19 February 2013

Nucleotide sequences of genes encoding allosamidin-sensitive and -insensitive chitinases produced by allosamidin-producing Streptomyces

scientific article published in September 2003

Preferential recognition of a microbial metabolite by human Vgamma2Vdelta2 T cells

scientific article published on 19 April 2007

Prenyltransferase substrate binding pocket flexibility and its application in isoprenoid profiling

scientific article published on 26 March 2009

Preparation of highly deuterated zeaxanthin, lycopene, and β-carotene from fully deuterated mevalonate using engineered Escherichia coli

scientific article published on 25 January 2005

Production of novel antioxidative prenyl naphthalen-ols by combinational bioconversion with dioxygenase PhnA1A2A3A4 and prenyltransferase NphB or SCO7190.

scientific article

Production of prenylated flavonoids in tomato fruits expressing a prenyltransferase gene from Streptomyces coelicolor A3(2)

scientific article published on 17 November 2010

Protein purification and function assignment of the epoxidase catalyzing the formation of fosfomycin

scientific article published in May 2001

Purification, crystallization and preliminary X-ray analysis of the regulatory subunit of aspartate kinase from Thermus thermophilus

scientific article published on 17 January 2007

Recent Advances in the Biosynthesis of Carbazoles Produced by Actinomycetes

scientific article published on 05 August 2020

Recent advances in the biosynthesis of nucleoside antibiotics

scientific article published on 25 September 2019

Regulation of resin acid synthesis in Pinus densiflora by differential transcription of genes encoding multiple 1-deoxy-D-xylulose 5-phosphate synthase and 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate reductase genes

scientific article published on 2 February 2009

Sequence of a P-methyltransferase-encoding gene isolated from a bialaphos-producing Streptomyces hygroscopicus.

scientific article published on May 1995

Structural Insight into concerted inhibition of alpha 2 beta 2-type aspartate kinase from Corynebacterium glutamicum

scientific article

Structural Insights into the CotB2-Catalyzed Cyclization of Geranylgeranyl Diphosphate to the Diterpene Cyclooctat-9-en-7-ol

scientific article

Structural and Mechanistic Insights into Terpene Synthases that Catalyze the Irregular Non-head-to-tail Coupling of Prenyl Substrates

scientific article published on 22 November 2018

Structural basis for leucine-induced allosteric activation of glutamate dehydrogenase.

scientific article published on 6 September 2011

Structural basis for the alteration of coenzyme specificity in a malate dehydrogenase mutant

scientific article published on 30 June 2006

Structural basis for the promiscuous biosynthetic prenylation of aromatic natural products

scientific article

Structural insight into amino group-carrier protein-mediated lysine biosynthesis: crystal structure of the LysZ·LysW complex from Thermus thermophilus.

scientific article published on 12 November 2014

Structure and Mechanism of the Monoterpene Cyclolavandulyl Diphosphate Synthase that Catalyzes Consecutive Condensation and Cyclization

scientific article published on 18 September 2017

Structure and function of an ancestral-type β-decarboxylating dehydrogenase from Thermococcus kodakarensis

scientific article published on 9 November 2016

Structure of the quinoline N-hydroxylating cytochrome P450 RauA, an essential enzyme that confers antibiotic activity on aurachin alkaloids

scientific article

Studies on the biosynthesis of fosfomycin. 2. Conversion of 2-hydoxypropylphosphonic acid to fosfomycin by blocked mutants of Streptomyces wedmorensis ⬇️

scientific article published on 01 November 1991

Studies on the biosynthesis of fosfomycin. 4. The biosynthetic origin of the methyl group of fosfomycin ⬇️

scientific article published on November 1, 1992

Terpene synthases are widely distributed in bacteria.

scientific article

The ABBA family of aromatic prenyltransferases: broadening natural product diversity

scientific article

The Amipurimycin and Miharamycin Biosynthetic Gene Clusters: Unraveling the Origins of 2-Aminopurinyl Peptidyl Nucleoside Antibiotics ⬇️

scientific article published on 31 May 2019

The herbicide ketoclomazone inhibits 1-deoxy-D-xylulose 5-phosphate synthase in the 2-C-methyl-D-erythritol 4-phosphate pathway and shows antibacterial activity against Haemophilus influenzae

scientific article published on September 2010

Two ATP-Binding Cassette Transporters Involved in (S)-2-Aminoethyl-Cysteine Uptake in Thermus thermophilus

scientific article

Two copies of 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase (CMK) gene in Ginkgo biloba: molecular cloning and functional characterization

scientific article published on 31 July 2008

Two distinct pathways for essential metabolic precursors for isoprenoid biosynthesis ⬇️

scientific article published on January 1, 2012

Unprecedented acetoacetyl-coenzyme A synthesizing enzyme of the thiolase superfamily involved in the mevalonate pathway.

scientific article

fldA is an essential gene required in the 2-C-methyl-D-erythritol 4-phosphate pathway for isoprenoid biosynthesis.

scientific article published in July 2005

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