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List of works by Kei Fujiwara

Artificial Cell Fermentation as a Platform for Highly Efficient Cascade Conversion

scientific article published on 20 December 2017

Biochemical Preparation of Cell Extract for Cell-Free Protein Synthesis without Physical Disruption

scientific article

Cell-Sized confinement in microspheres accelerates the reaction of gene expression.

scientific article published on 22 February 2012

Cell-sized confinement controls generation and stability of a protein wave for spatiotemporal regulation in cells

scientific article published on 30 July 2019

Condensation of an additive-free cell extract to mimic the conditions of live cells.

scientific article

Conformational equilibrium of MinE regulates the allowable concentration ranges of a protein wave for cell division

scientific article published on 27 May 2020

Conversion of a chaperonin GroEL-independent protein into an obligate substrate

scientific article published on 6 October 2014

DNA cytoskeleton for stabilizing artificial cells

scientific article published on 26 June 2017

Droplet-Shooting and Size-Filtration (DSSF) Method for Synthesis of Cell-Sized Liposomes with Controlled Lipid Compositions

scientific article published on 25 July 2015

Filamentous morphology in GroE-depleted Escherichia coli induced by impaired folding of FtsE.

scientific article published on 8 June 2007

Generation of giant unilamellar liposomes containing biomacromolecules at physiological intracellular concentrations using hypertonic conditions.

scientific article

Generation of monodisperse cell-sized microdroplets using a centrifuge-based axisymmetric co-flowing microfluidic device

scientific article published on 22 October 2014

Human-derived fusogenic peptides for the intracellular delivery of proteins

scientific article published on 3 April 2017

Identification of novel in vivo obligate GroEL/ES substrates based on data from a cell-free proteomics approach.

scientific article

In vitro transcription-translation using bacterial genome as a template to reconstitute intracellular profile.

scientific article published on 30 August 2017

Increasing Elasticity through Changes in the Secondary Structure of Gelatin by Gelation in a Microsized Lipid Space.

scientific article published on 15 March 2018

Introducing micrometer-sized artificial objects into live cells: a method for cell-giant unilamellar vesicle electrofusion

scientific article

In vitro selection of bispecific diabody fragments using covalent bicistronic DNA display

scientific article

Liposomal internal viscosity affects the fate of membrane deformation induced by hypertonic treatment

scientific article

Mechanism of methionine synthase overexpression in chaperonin-depleted Escherichia coli.

scientific article

Microfluidic screening system based on boron-doped diamond electrodes and dielectrophoretic sorting for directed evolution of NAD(P)-dependent oxidoreductases

scientific article published on 27 January 2020

Multiple patterns of polymer gels in microspheres due to the interplay among phase separation, wetting, and gelation.

scientific article

PURE mRNA display for in vitro selection of single-chain antibodies

scientific article published on 28 December 2015

Peptide sequences converting polyglutamine into a prion in yeast.

scientific article published on 9 December 2014

Physicochemical analysis from real-time imaging of liposome tubulation reveals the characteristics of individual F-BAR domain proteins.

scientific article

Reconstitution of intracellular environments in vitro and in artificial cells

scientific article

Regulation of spatiotemporal patterning in artificial cells by a defined protein expression system

scientific article published on 16 October 2019

Transcription–translation of the <i>Escherichia coli</i> genome within artificial cells

scientific article published in 2021

Universal glass-forming behavior of in vitro and living cytoplasm

scientific article

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