List of works - David Tscharke

A consensus epitope prediction approach identifies the breadth of murine T(CD8+)-cell responses to vaccinia virus

scientific article

Altered CD8(+) T cell immunodominance after vaccinia virus infection and the naive repertoire in inbred and F(1) mice.

scientific article

An intact signal peptide on dengue virus E protein enhances immunogenicity for CD8(+) T cells and antibody when expressed from modified vaccinia Ankara

scientific article published on 14 April 2014

An intradermal model for vaccinia virus pathogenesis in mice.

scientific article published in January 2012

An ultrastructural investigation of tumors undergoing regression mediated by immunotherapy

scientific article published on 14 December 2017

Analysis of A47, an immunoprevalent protein of vaccinia virus, leads to a reevaluation of the total antiviral CD8+ T cell response.

scientific article

Analyzing CD8 T cells in mouse models of poxvirus infection

scientific article

Bacillus cereus non-haemolytic enterotoxin activates the NLRP3 inflammasome ⬇️

scientific article published on 06 February 2020

Benchmarking predictions of MHC class I restricted T cell epitopes in a comprehensively studied model system

scientific article published on 26 May 2020

CRISPR/Cas9-Based Genome Editing of HSV

scientific article published on 01 January 2020

Comparative analysis of the complete genome sequence of the California MSW strain of myxoma virus reveals potential host adaptations

scientific article published in November 2013

Definition of epitopes and antigens recognized by vaccinia specific immune responses: their conservation in variola virus sequences, and use as a model system to study complex pathogens

scientific article

Delayed control of herpes simplex virus infection and impaired CD4(+) T-cell migration to the skin in mouse models of DOCK8 deficiency

scientific article published on 17 March 2015

Deletion of gene A41L enhances vaccinia virus immunogenicity and vaccine efficacy.

scientific article

Dissociation between epitope hierarchy and immunoprevalence in CD8 responses to vaccinia virus western reserve

scientific article published on June 2008

Dual-specific Chimeric Antigen Receptor T Cells and an Indirect Vaccine Eradicate a Variety of Large Solid Tumors in an Immunocompetent, Self-antigen Setting.

scientific article published on 13 December 2016

Ectromelia virus N1L is essential for virulence but not dissemination in a classical model of mousepox.

scientific article published on 16 November 2016

Effective Priming of Herpes Simplex Virus-Specific CD8+ T Cells In Vivo Does Not Require Infected Dendritic Cells ⬇️

scientific article published on 15 November 2017

Engineering herpes simplex viruses by infection-transfection methods including recombination site targeting by CRISPR/Cas9 nucleases.

scientific article

Engineering recombinant poxviruses using a compact GFP-blasticidin resistance fusion gene for selection.

scientific article published on 10 November 2010

Evolutionary history and attenuation of myxoma virus on two continents

scientific article published in 2012

Extent of Systemic Spread Determines CD8+ T Cell Immunodominance for Laboratory Strains, Smallpox Vaccines, and Zoonotic Isolates of Vaccinia Virus.

scientific article

From mice to humans - murine intelligence for human CD8+ T cell vaccine design

scientific article

Functional analysis of the short isoform of orf virus protein OV20.0.

scientific article

Functional paralysis of human natural killer cells by alphaherpesviruses.

scientific article published on 13 June 2019

Genome scale evolution of myxoma virus reveals host-pathogen adaptation and rapid geographic spread

scientific article published on 25 September 2013

HLA class I-restricted responses to vaccinia recognize a broad array of proteins mainly involved in virulence and viral gene regulation

scientific article

HLA-A0201, HLA-A1101, and HLA-B*0702 transgenic mice recognize numerous poxvirus determinants from a wide variety of viral gene products

scientific article published in October 2005

Herpes Simplex Virus Latency Is Noisier the Closer We Look

scientific article published on 31 January 2020

IL-4 and IL-13 mediated down-regulation of CD8 expression levels can dampen anti-viral CD8⁺ T cell avidity following HIV-1 recombinant pox viral vaccination.

scientific article published on 6 August 2013

Identification of poxvirus CD8+ T cell determinants to enable rational design and characterization of smallpox vaccines

scientific article published on 28 December 2004

Immunodomination during peripheral vaccinia virus infection.

scientific article

Immunology by numbers: quantitation of antigen presentation completes the quantitative milieu of systems immunology!

scientific article

Increasing antigen presentation on HSV-1-infected cells increases lesion size but does not alter neural infection or latency.

scientific article

Influenza A Virus Infection Induces Viral and Cellular Defective Ribosomal Products Encoded by Alternative Reading Frames

scientific article published on 15 May 2019

Inside the professionals

scientific article published in Nature

Kinetic analysis of a complete poxvirus transcriptome reveals an immediate-early class of genes

scholarly article

Kinetics of antigen expression and epitope presentation during virus infection.

scientific article

Linear fidelity in quantification of anti-viral CD8+ T cells

scientific article

Local proliferation maintains a stable pool of tissue-resident memory T cells after antiviral recall responses

scientific article published on 8 January 2018

Lytic Promoters Express Protein during Herpes Simplex Virus Latency.

scientific article

Lytic gene expression is frequent in HSV-1 latent infection and correlates with the engagement of a cell-intrinsic transcriptional response

scientific article

Modified Vaccinia Virus Ankara Can Induce Optimal CD8+ T Cell Responses to Directly Primed Antigens Depending on Vaccine Design

scientific article published on 15 October 2019

Most viral peptides displayed by class I MHC on infected cells are immunogenic

scientific article

Overlapping Peptides Elicit Distinct CD8+ T Cell Responses following Influenza A Virus Infection

scientific article published on 31 August 2020

Poxvirus CD8+ T-cell determinants and cross-reactivity in BALB/c mice

scientific article

Quantification of epitope abundance reveals the effect of direct and cross-presentation on influenza CTL responses

scientific article published on 28 June 2019

Quantifying epitope presentation using mass spectrometry

scientific article published on 25 June 2015

Rapid poxvirus engineering using CRISPR/Cas9 as a selection tool

scientific article published on 03 November 2020

Reduced interleukin-4 receptor α expression on CD8+ T cells correlates with higher quality anti-viral immunity

scientific article

Reduction of vector gene expression increases foreign antigen-specific CD8+ T-cell priming

scientific article published on 01 September 2007

Redundancy complicates the definition of essential genes for vaccinia virus

scientific article

Regulation of protein translation through mRNA structure influences MHC class I loading and T cell recognition.

scholarly article

Regulatory T cells suppress CD8+ T cell responses induced by direct priming and cross-priming and moderate immunodominance disparities.

scientific article published in March 2005

Reply to Satheshkumar and Moss: Poxvirus transcriptome analysis.

scholarly article

Simultaneous Quantification of Viral Antigen Expression Kinetics Using Data-Independent (DIA) Mass Spectrometry.

scientific article

Sizing up the key determinants of the CD8(+) T cell response

scientific article published on 09 October 2015

Strikingly poor CD8+ T-cell immunogenicity of vaccinia virus strain MVA in BALB/c mice.

scientific article

Surprisingly Effective Priming of CD8+ T cells by Heat-Inactivated Vaccinia Virus Virions

scientific article published on 05 August 2020

Systemic toll-like receptor ligation and selective killing of dendritic cell subsets fail to dissect priming pathways for anti-vaccinia virus CD8⁺ T cells

scientific article published on 28 August 2013

T cells bite the hand that feeds them

scientific article published on 01 June 2003

The Epstein-Barr Virus Glycoprotein gp150 Forms an Immune-Evasive Glycan Shield at the Surface of Infected Cells

scientific article

The cellular redox environment alters antigen presentation

scientific article

The developmental pathway for CD103(+)CD8+ tissue-resident memory T cells of skin.

scientific article published on 27 October 2013

The ectromelia virus SPI-2 protein causes lethal mousepox by preventing NK cell responses

scientific article

The primary immune response to Vaccinia virus vaccination includes cells with a distinct cytotoxic effector CD4 T-cell phenotype.

scientific article published on 14 September 2016

The vaccinia virus kelch-like protein C2L affects calcium-independent adhesion to the extracellular matrix and inflammation in a murine intradermal model

scientific article published in September 2003

Tissue-resident memory T cells in tissue homeostasis, persistent infection, and cancer surveillance.

scientific article

Truncation of gene F5L partially masks rescue of vaccinia virus strain MVA growth on mammalian cells by restricting plaque size.

scientific article published on 21 October 2013

Uncovering the interplay between CD8, CD4 and antibody responses to complex pathogens.

scientific article

Use of CD107-based cell sorting ex vivo to enrich subdominant CD8+ T cells in culture.

scientific article

Vaccinia virus CD8+ T-cell dominance hierarchies cannot be altered by prior immunization with individual peptides.

scientific article published on 17 June 2009

Vaccinia virus F5 is required for normal plaque morphology in multiple cell lines but not replication in culture or virulence in mice

scientific article

List of works by David Tscharke

A consensus epitope prediction approach identifies the breadth of murine T(CD8+)-cell responses to vaccinia virus

Altered CD8(+) T cell immunodominance after vaccinia virus infection and the naive repertoire in inbred and F(1) mice.

An intact signal peptide on dengue virus E protein enhances immunogenicity for CD8(+) T cells and antibody when expressed from modified vaccinia Ankara

An intradermal model for vaccinia virus pathogenesis in mice.

An ultrastructural investigation of tumors undergoing regression mediated by immunotherapy

Analysis of A47, an immunoprevalent protein of vaccinia virus, leads to a reevaluation of the total antiviral CD8+ T cell response.

Analyzing CD8 T cells in mouse models of poxvirus infection

Bacillus cereus non-haemolytic enterotoxin activates the NLRP3 inflammasome ⬇️

Benchmarking predictions of MHC class I restricted T cell epitopes in a comprehensively studied model system

CRISPR/Cas9-Based Genome Editing of HSV

Comparative analysis of the complete genome sequence of the California MSW strain of myxoma virus reveals potential host adaptations

Definition of epitopes and antigens recognized by vaccinia specific immune responses: their conservation in variola virus sequences, and use as a model system to study complex pathogens

Delayed control of herpes simplex virus infection and impaired CD4(+) T-cell migration to the skin in mouse models of DOCK8 deficiency

Deletion of gene A41L enhances vaccinia virus immunogenicity and vaccine efficacy.

Dissociation between epitope hierarchy and immunoprevalence in CD8 responses to vaccinia virus western reserve

Dual-specific Chimeric Antigen Receptor T Cells and an Indirect Vaccine Eradicate a Variety of Large Solid Tumors in an Immunocompetent, Self-antigen Setting.

Ectromelia virus N1L is essential for virulence but not dissemination in a classical model of mousepox.

Effective Priming of Herpes Simplex Virus-Specific CD8+ T Cells In Vivo Does Not Require Infected Dendritic Cells ⬇️

Engineering herpes simplex viruses by infection-transfection methods including recombination site targeting by CRISPR/Cas9 nucleases.

Engineering recombinant poxviruses using a compact GFP-blasticidin resistance fusion gene for selection.

Evolutionary history and attenuation of myxoma virus on two continents

Extent of Systemic Spread Determines CD8+ T Cell Immunodominance for Laboratory Strains, Smallpox Vaccines, and Zoonotic Isolates of Vaccinia Virus.

From mice to humans - murine intelligence for human CD8+ T cell vaccine design

Functional analysis of the short isoform of orf virus protein OV20.0.

Functional paralysis of human natural killer cells by alphaherpesviruses.

Genome scale evolution of myxoma virus reveals host-pathogen adaptation and rapid geographic spread

HLA class I-restricted responses to vaccinia recognize a broad array of proteins mainly involved in virulence and viral gene regulation

HLA-A*0201, HLA-A*1101, and HLA-B*0702 transgenic mice recognize numerous poxvirus determinants from a wide variety of viral gene products

Herpes Simplex Virus Latency Is Noisier the Closer We Look

IL-4 and IL-13 mediated down-regulation of CD8 expression levels can dampen anti-viral CD8⁺ T cell avidity following HIV-1 recombinant pox viral vaccination.

Identification of poxvirus CD8+ T cell determinants to enable rational design and characterization of smallpox vaccines

Immunodomination during peripheral vaccinia virus infection.

Immunology by numbers: quantitation of antigen presentation completes the quantitative milieu of systems immunology!

Increasing antigen presentation on HSV-1-infected cells increases lesion size but does not alter neural infection or latency.

Influenza A Virus Infection Induces Viral and Cellular Defective Ribosomal Products Encoded by Alternative Reading Frames

Inside the professionals

Kinetic analysis of a complete poxvirus transcriptome reveals an immediate-early class of genes

Kinetics of antigen expression and epitope presentation during virus infection.

Linear fidelity in quantification of anti-viral CD8+ T cells

Local proliferation maintains a stable pool of tissue-resident memory T cells after antiviral recall responses

Lytic Promoters Express Protein during Herpes Simplex Virus Latency.

Lytic gene expression is frequent in HSV-1 latent infection and correlates with the engagement of a cell-intrinsic transcriptional response

Modified Vaccinia Virus Ankara Can Induce Optimal CD8+ T Cell Responses to Directly Primed Antigens Depending on Vaccine Design

Most viral peptides displayed by class I MHC on infected cells are immunogenic

Overlapping Peptides Elicit Distinct CD8+ T Cell Responses following Influenza A Virus Infection

Poxvirus CD8+ T-cell determinants and cross-reactivity in BALB/c mice

Quantification of epitope abundance reveals the effect of direct and cross-presentation on influenza CTL responses

Quantifying epitope presentation using mass spectrometry

Rapid poxvirus engineering using CRISPR/Cas9 as a selection tool

Reduced interleukin-4 receptor α expression on CD8+ T cells correlates with higher quality anti-viral immunity

Reduction of vector gene expression increases foreign antigen-specific CD8+ T-cell priming

Redundancy complicates the definition of essential genes for vaccinia virus

Regulation of protein translation through mRNA structure influences MHC class I loading and T cell recognition.

Regulatory T cells suppress CD8+ T cell responses induced by direct priming and cross-priming and moderate immunodominance disparities.

Reply to Satheshkumar and Moss: Poxvirus transcriptome analysis.

Simultaneous Quantification of Viral Antigen Expression Kinetics Using Data-Independent (DIA) Mass Spectrometry.

Sizing up the key determinants of the CD8(+) T cell response

Strikingly poor CD8+ T-cell immunogenicity of vaccinia virus strain MVA in BALB/c mice.

Surprisingly Effective Priming of CD8+ T cells by Heat-Inactivated Vaccinia Virus Virions

Systemic toll-like receptor ligation and selective killing of dendritic cell subsets fail to dissect priming pathways for anti-vaccinia virus CD8⁺ T cells

T cells bite the hand that feeds them

The Epstein-Barr Virus Glycoprotein gp150 Forms an Immune-Evasive Glycan Shield at the Surface of Infected Cells

The cellular redox environment alters antigen presentation

The developmental pathway for CD103(+)CD8+ tissue-resident memory T cells of skin.

The ectromelia virus SPI-2 protein causes lethal mousepox by preventing NK cell responses

The primary immune response to Vaccinia virus vaccination includes cells with a distinct cytotoxic effector CD4 T-cell phenotype.

The vaccinia virus kelch-like protein C2L affects calcium-independent adhesion to the extracellular matrix and inflammation in a murine intradermal model

Tissue-resident memory T cells in tissue homeostasis, persistent infection, and cancer surveillance.

Truncation of gene F5L partially masks rescue of vaccinia virus strain MVA growth on mammalian cells by restricting plaque size.

Uncovering the interplay between CD8, CD4 and antibody responses to complex pathogens.

Use of CD107-based cell sorting ex vivo to enrich subdominant CD8+ T cells in culture.

Vaccinia virus CD8+ T-cell dominance hierarchies cannot be altered by prior immunization with individual peptides.

Vaccinia virus F5 is required for normal plaque morphology in multiple cell lines but not replication in culture or virulence in mice

HLA-A0201, HLA-A1101, and HLA-B*0702 transgenic mice recognize numerous poxvirus determinants from a wide variety of viral gene products