List of works - Fei Liao

A Practical System for High-Throughput Screening of Mutants of Bacillus fastidiosus Uricase

scientific article

A new linearly-combined bi-exponential model for kinetic analysis of the isometric relaxation process of Bufo gastrocnemius under electric stimulation in vitro

scientific article

A new practical system for evaluating the pharmacological properties of uricase as a potential drug for hyperuricemia

scientific article published on 30 November 2010

An improved malachite green assay of phosphate: mechanism and application

scientific article published on 9 November 2010

Approximated maximum adsorption of His-tagged enzyme/mutants on Ni2+-NTA for comparison of specific activities

scientific article published on 24 December 2014

Assay of serum arylesterase activity by fitting to the reaction curve with an integrated rate equation

scientific article published in December 2001

Catalytic Mechanisms for Cofactor-Free Oxidase-Catalyzed Reactions: Reaction Pathways of Uricase-Catalyzed Oxidation and Hydration of Uric Acid.

scientific article published on 15 June 2017

Characterization of a uricase from Bacillus fastidious A.T.C.C. 26904 and its application to serum uric acid assay by a patented kinetic uricase method

scientific article published in September 2006

Characterization of alcohol dehydrogenase from permeabilized brewer's yeast cells immobilized on the derived attapulgite nanofibers.

scientific article

Classification of difference between inhibition constants of an inhibitor to facilitate identifying the inhibition type

scientific article

Comparison of activity indexes for recognizing enzyme mutants of higher activity with uricase as model ⬇️

scientific article published on April 17, 2013

Comparison of the Full-Length and 152~528 Truncate of Human Cyclic Nucleotide Phosphodiesterase 4B2 for the Characterization of Inhibitors

scientific article published on 01 January 2019

Correlation of serum arylesterase activity on phenylacetate estimated by the integrated method to common classical biochemical indexes of liver damage

scientific article published on April 2007

Crystal structure of Bacillus fastidious uricase reveals an unexpected folding of the C-terminus residues crucial for thermostability under physiological conditions

scientific article

Current status and future prospects of biomarkers in the diagnosis of hepatocellular carcinoma.

scientific article published on 22 September 2017

Data for high-throughput estimation of specific activities of enzyme/mutants in cell lysates through immunoturbidimetric assay of proteins.

scientific article published on 15 July 2017

Data for high-throughput screening of enzyme mutants by comparison of their activity ratios to an enzyme tag

scientific article published on 13 December 2019

Design of Bacillus fastidious Uricase Mutants Bearing Long Lagging Phases Before Exponential Decreases of Activities Under Physiological Conditions

scientific article published in 2021

Design, synthesis and evaluation of novel quinazoline-2,4-dione derivatives as chitin synthase inhibitors and antifungal agents

scientific article

Effects of industrial storage on the bioreduction capacity of brewer's yeast

scientific article

Effects of modification of amino groups with poly(ethylene glycol) on a recombinant uricase from Bacillus fastidiosus

scientific article published on 7 June 2010

Evaluation of a kinetic uricase method for serum uric acid assay by predicting background absorbance of uricase reaction solution with an integrated method

scientific article

Extracorporeal delivery of a therapeutic enzyme

scientific article published on August 2016

Facile Alkaline Lysis of Escherichia coli Cells in High-Throughput Mode for Screening Enzyme Mutants: Arylsulfatase as an Example

scientific article published on 22 February 2016

Facile characterization of the immobilization of streptavidin on magnetic submicron particles with a fluorescent probe of streptavidin.

scientific article published in June 2013

Facile one-step coating approach to magnetic submicron particles with poly(ethylene glycol) coats and abundant accessible carboxyl groups

scientific article

Facile spectrophotometric assay of molar equivalents of N-hydroxysuccinimide esters of monomethoxyl poly-(ethylene glycol) derivatives

scientific article published on 23 November 2012

Fluorometric titration approach for calibration of quantity of binding site of purified monoclonal antibody recognizing epitope/hapten nonfluorescent at 340 nm

scientific article published on 04 June 2014

Fluorometric titration assay of affinity of tight-binding nonfluorescent inhibitor of glutathione S-transferase

scientific article published on 28 October 2014

Glutathione S‑transferase isozyme alpha 1 is predominantly involved in the cisplatin resistance of common types of solid cancer

scientific article published on 09 November 2018

High-throughput estimation of specific activities of enzyme/mutants in cell lysates through immunoturbidimetric assay of proteins

scientific article published on 16 May 2017

Homogeneous competitive assay of ligand affinities based on quenching fluorescence of tyrosine/tryptophan residues in a protein via Főrster-resonance-energy-transfer

scientific article published on 14 August 2010

Homogeneous noncompetitive assay of protein via Förster-resonance-energy-transfer with tryptophan residue(s) as intrinsic donor(s) and fluorescent ligand as acceptor.

scientific article published on 17 June 2009

Integrated rate equation considering product inhibition and its application to kinetic assay of serum ethanol

scientific article published in April 2007

Ion-exchange medium coated with abundant small zwitterions for the purification of soluble proteins

scientific article published on 23 September 2020

Kinetic analysis of the lactate-dehydrogenase-coupled reaction process and measurement of alanine transaminase by an integration strategy

scientific article published on January 2010

Kinetic analysis of γ-glutamyltransferase reaction process for measuring activity via an integration strategy at low concentrations of γ-glutamyl p-nitroaniline

scientific article

Kinetic substrate quantification by fitting the enzyme reaction curve to the integrated Michaelis-Menten equation

scientific article published on 28 February 2003

Microplate-based method to screen inhibitors of isozymes of cyclic nucleotide phosphodiesterase fused to SUMO.

scientific article published on 11 February 2014

Resonant-Mie-scattering of aggregates of phosphomolybdate and papaverine for measuring activities and screening inhibitors of cyclic nucleotide phosphodiesterase isozymes

scientific article published on 16 October 2013

Reversible inactivation of an intracellular uricase from Bacillus fastidiosus via dissociation of homotetramer into homodimers in solutions of low ionic strength

scientific article published on 7 September 2009

Selective and sensitive homogenous assay of serum albumin with 1-anilinonaphthalene-8-sulphonate as a biosensor

scientific article published on 26 April 2014

Significance of combined tests of serum golgi glycoprotein 73 and other biomarkers in diagnosis of small primary hepatocellular carcinoma

scientific article published in January 2015

Site-specific PEGylation of therapeutic proteins via optimization of both accessible reactive amino acid residues and PEG derivatives

scientific article published on August 2012

Spectrophotometric-Dual-Enzyme-Simultaneous Assay in One Reaction Solution: Chemometrics and Experimental Models ⬇️

scientific article published on January 25, 2013

Striking Effects of Storage Buffers on Apparent Half-Lives of the Activity of Pseudomonas aeruginosa Arylsulfatase

scientific article published on 2 July 2016

Synthesis and biological evaluation of novel phosphoramidate derivatives of coumarin as chitin synthase inhibitors and antifungal agents

scientific article published on 23 November 2015

The comparison of the estimation of enzyme kinetic parameters by fitting reaction curve to the integrated Michaelis-Menten rate equations of different predictor variables

scientific article published in January 2005

Uricases as therapeutic agents to treat refractory gout: Current states and future directions

scientific article

[Characterization of Mycobacterium tuberculosis dihydrofolate reductase immobilized on magnetic nanoparticles]

scientific article published on 01 March 2019

List of works by Fei Liao

A Practical System for High-Throughput Screening of Mutants of Bacillus fastidiosus Uricase

A new linearly-combined bi-exponential model for kinetic analysis of the isometric relaxation process of Bufo gastrocnemius under electric stimulation in vitro

A new practical system for evaluating the pharmacological properties of uricase as a potential drug for hyperuricemia

An improved malachite green assay of phosphate: mechanism and application

Approximated maximum adsorption of His-tagged enzyme/mutants on Ni2+-NTA for comparison of specific activities

Assay of serum arylesterase activity by fitting to the reaction curve with an integrated rate equation

Catalytic Mechanisms for Cofactor-Free Oxidase-Catalyzed Reactions: Reaction Pathways of Uricase-Catalyzed Oxidation and Hydration of Uric Acid.

Characterization of a uricase from Bacillus fastidious A.T.C.C. 26904 and its application to serum uric acid assay by a patented kinetic uricase method

Characterization of alcohol dehydrogenase from permeabilized brewer's yeast cells immobilized on the derived attapulgite nanofibers.

Classification of difference between inhibition constants of an inhibitor to facilitate identifying the inhibition type

Comparison of activity indexes for recognizing enzyme mutants of higher activity with uricase as model ⬇️

Comparison of the Full-Length and 152~528 Truncate of Human Cyclic Nucleotide Phosphodiesterase 4B2 for the Characterization of Inhibitors

Correlation of serum arylesterase activity on phenylacetate estimated by the integrated method to common classical biochemical indexes of liver damage

Crystal structure of Bacillus fastidious uricase reveals an unexpected folding of the C-terminus residues crucial for thermostability under physiological conditions

Current status and future prospects of biomarkers in the diagnosis of hepatocellular carcinoma.

Data for high-throughput estimation of specific activities of enzyme/mutants in cell lysates through immunoturbidimetric assay of proteins.

Data for high-throughput screening of enzyme mutants by comparison of their activity ratios to an enzyme tag

Design of Bacillus fastidious Uricase Mutants Bearing Long Lagging Phases Before Exponential Decreases of Activities Under Physiological Conditions

Design, synthesis and evaluation of novel quinazoline-2,4-dione derivatives as chitin synthase inhibitors and antifungal agents

Effects of industrial storage on the bioreduction capacity of brewer's yeast

Effects of modification of amino groups with poly(ethylene glycol) on a recombinant uricase from Bacillus fastidiosus

Evaluation of a kinetic uricase method for serum uric acid assay by predicting background absorbance of uricase reaction solution with an integrated method

Extracorporeal delivery of a therapeutic enzyme

Facile Alkaline Lysis of Escherichia coli Cells in High-Throughput Mode for Screening Enzyme Mutants: Arylsulfatase as an Example

Facile characterization of the immobilization of streptavidin on magnetic submicron particles with a fluorescent probe of streptavidin.

Facile one-step coating approach to magnetic submicron particles with poly(ethylene glycol) coats and abundant accessible carboxyl groups

Facile spectrophotometric assay of molar equivalents of N-hydroxysuccinimide esters of monomethoxyl poly-(ethylene glycol) derivatives

Fluorometric titration approach for calibration of quantity of binding site of purified monoclonal antibody recognizing epitope/hapten nonfluorescent at 340 nm

Fluorometric titration assay of affinity of tight-binding nonfluorescent inhibitor of glutathione S-transferase

Glutathione S‑transferase isozyme alpha 1 is predominantly involved in the cisplatin resistance of common types of solid cancer

High-throughput estimation of specific activities of enzyme/mutants in cell lysates through immunoturbidimetric assay of proteins

Homogeneous competitive assay of ligand affinities based on quenching fluorescence of tyrosine/tryptophan residues in a protein via Főrster-resonance-energy-transfer

Homogeneous noncompetitive assay of protein via Förster-resonance-energy-transfer with tryptophan residue(s) as intrinsic donor(s) and fluorescent ligand as acceptor.

Integrated rate equation considering product inhibition and its application to kinetic assay of serum ethanol

Ion-exchange medium coated with abundant small zwitterions for the purification of soluble proteins

Kinetic analysis of the lactate-dehydrogenase-coupled reaction process and measurement of alanine transaminase by an integration strategy

Kinetic analysis of γ-glutamyltransferase reaction process for measuring activity via an integration strategy at low concentrations of γ-glutamyl p-nitroaniline

Kinetic substrate quantification by fitting the enzyme reaction curve to the integrated Michaelis-Menten equation

Microplate-based method to screen inhibitors of isozymes of cyclic nucleotide phosphodiesterase fused to SUMO.

Resonant-Mie-scattering of aggregates of phosphomolybdate and papaverine for measuring activities and screening inhibitors of cyclic nucleotide phosphodiesterase isozymes

Reversible inactivation of an intracellular uricase from Bacillus fastidiosus via dissociation of homotetramer into homodimers in solutions of low ionic strength

Selective and sensitive homogenous assay of serum albumin with 1-anilinonaphthalene-8-sulphonate as a biosensor

Significance of combined tests of serum golgi glycoprotein 73 and other biomarkers in diagnosis of small primary hepatocellular carcinoma

Site-specific PEGylation of therapeutic proteins via optimization of both accessible reactive amino acid residues and PEG derivatives

Spectrophotometric-Dual-Enzyme-Simultaneous Assay in One Reaction Solution: Chemometrics and Experimental Models ⬇️

Striking Effects of Storage Buffers on Apparent Half-Lives of the Activity of Pseudomonas aeruginosa Arylsulfatase

Synthesis and biological evaluation of novel phosphoramidate derivatives of coumarin as chitin synthase inhibitors and antifungal agents

The comparison of the estimation of enzyme kinetic parameters by fitting reaction curve to the integrated Michaelis-Menten rate equations of different predictor variables

Uricases as therapeutic agents to treat refractory gout: Current states and future directions

[Characterization of Mycobacterium tuberculosis dihydrofolate reductase immobilized on magnetic nanoparticles]