List of works - Stefano Bettati

A Trivalent Enzymatic System for Uricolytic Therapy of HPRT Deficiency and Lesch-Nyhan Disease.

scientific article

A tertiary two-state allosteric model for hemoglobin

scientific article published in July 2002

A two-step process controls the formation of the bienzyme cysteine synthase complex

scientific article

Activation of an anti-bacterial toxin by the biosynthetic enzyme CysK: mechanism of binding, interaction specificity and competition with cysteine synthase.

scientific article published on 18 August 2017

Allosteric communication between alpha and beta subunits of tryptophan synthase: modelling the open-closed transition of the alpha subunit

scientific article published on 18 April 2006

Allosteric communication of tryptophan synthase. Functional and regulatory properties of the beta S178P mutant

scientific article

Allosteric mechanism of haemoglobin: rupture of salt-bridges raises the oxygen affinity of the T-structure 1 1Edited by D. Rees

scientific article published on 01 August 1998

Asymmetry of the active site loop conformation between subunits of glutamate-1-semialdehyde aminomutase in solution

scientific article published on 31 July 2013

CO Rebinding Kinetics to Myoglobin- and R-State-Hemoglobin-Doped Silica Gels in the Presence of Glycerol

scientific article published in June 2004

Catalysis and Structure of Zebrafish Urate Oxidase Provide Insights into the Origin of Hyperuricemia in Hominoids

scientific article

Catalytic competence of O-acetylserine sulfhydrylase in the crystal probed by polarized absorption microspectrophotometry

scientific article

Circular dichroism spectroscopy of tertiary and quaternary conformations of human hemoglobin entrapped in wet silica gels

scientific article

Combination of SAXS and Protein Painting Discloses the Three-Dimensional Organization of the Bacterial Cysteine Synthase Complex, a Potential Target for Enhancers of Antibiotic Action

scientific article published on 21 October 2019

Confinement and crowding effects on tryptophan synthase α2β2complex

scholarly article by Barbara Pioselli published in March 2005

Conformational probes of O-acetylserine sulfhydrylase: fluorescence of tryptophans 50 and 161

Cooperative Oxygen Binding toScapharca inaequivalvisHemoglobin in the Crystal

scientific article published in Journal of Biological Chemistry

Correlation of protein functional properties in the crystal and in solution: the case study of T-state hemoglobin

scientific article published on July 2002

Cryo-EM structures of staphylococcal IsdB bound to human hemoglobin reveal the process of heme extraction

scientific article published on 31 March 2022

Cyclopropane-1,2-dicarboxylic acids as new tools for the biophysical investigation of O-acetylserine sulfhydrylases by fluorimetric methods and saturation transfer difference (STD) NMR.

scientific article published on 31 August 2016

Design of O -Acetylserine Sulfhydrylase Inhibitors by Mimicking Nature

scientific article

Diatom Allantoin Synthase Provides Structural Insights into Natural Fusion Protein Therapeutics

scientific article published on 28 June 2018

Dynamics of green fluorescent protein mutant2 in solution, on spin-coated glasses, and encapsulated in wet silica gels

scientific article published on May 2002

Effect of the point mutation H148G on GFPmut2 unfolding kinetics by fluorescence spectroscopy

scientific article published on 13 April 2011

Engineering hemoglobin to enable homogenous PEGylation without modifying protein functionality

scientific article published on 15 June 2020

Environment effects on the oscillatory unfolding kinetics of GFP.

scientific article

Evidence for Two Geminate Rebinding States Following Laser Photolysis of R State Hemoglobin Encapsulated in Wet Silica Gels

scientific article published in June 2005

Evidence of discrete substates and unfolding pathways in green fluorescent protein

scientific article

Evolution of allosteric models for hemoglobin

scientific article published on August 2007

Exercise capacity assessment in patients undergoing lung resection.

scientific article published on 09 January 2009

Experimental basis for a new allosteric model for multisubunit proteins

scientific article

Experiments on Hemoglobin in Single Crystals and Silica Gels Distinguish among Allosteric Models.

scientific article

Exploring O-acetylserine sulfhydrylase-B isoenzyme from Salmonella typhimurium by fluorescence spectroscopy

scientific article published on 16 October 2010

Exploring the pyridoxal 5′-phosphate-dependent enzymes

article

Functional characterization of heme proteins encapsulated in wet nanoporous silica gels.

scientific article

Geminate rebinding in R-state hemoglobin: kinetic and computational evidence for multiple hydrophobic pockets

scientific article

Glutamine 89 is a key residue in the allosteric modulation of human serine racemase activity by ATP.

scientific article published on 13 June 2018

Haemoglobin-based oxygen carriers: research and reality towards an alternative to blood transfusions

scientific article

Hemocyanin from E. californicum encapsulated in silica gels: oxygen binding and conformational states

scientific article published on 25 April 2007

Hemoglobin Reactivity and Regulation

Hemoglobin, an "evergreen" red protein

scientific article published on 08 April 2009

High and low oxygen affinity conformations of T state hemoglobin

scientific article

Identification of the geometric requirements for allosteric communication between the alpha- and beta-subunits of tryptophan synthase

scientific article published on 3 February 2005

Identification of the structural determinants for the stability of substrate and aminoacrylate external Schiff bases in O-acetylserine sulfhydrylase-A.

scientific article published in July 2010

Immobilization of Allantoinase for the Development of an Optical Biosensor of Oxidative Stress States

scientific article published on 29 December 2019

Insight into GFPmut2 pH Dependence by Single Crystal Microspectrophotometry and X-ray Crystallography

scientific article published on 17 September 2018

Interaction of human hemoglobin and semi-hemoglobins with the Staphylococcus aureus hemophore IsdB: a kinetic and mechanistic insight

scientific article published on 09 December 2019

Interaction of serine acetyltransferase with O-acetylserine sulfhydrylase active site: evidence from fluorescence spectroscopy

scientific article published on 29 June 2005

Iron Metabolism at the Interface between Host and Pathogen: From Nutritional Immunity to Antibacterial Development

scientific article published on 20 March 2020

Kinetics of acid-induced spectral changes in the GFPmut2 chromophore

scientific article published in January 2005

Ligand reactivity and allosteric regulation of hemoglobin-based oxygen carriers.

scientific article

Ligand-induced tertiary relaxations during the T-to-R quaternary transition in hemoglobin

scientific article

Lung metastasis resection of adenoid cystic carcinoma of salivary glands

scientific article published on 14 March 2008

Modulation of Escherichia coli serine acetyltransferase catalytic activity in the cysteine synthase complex

scientific article published on 24 March 2017

Molecular insights into dimerization inhibition of c-Maf transcription factor

scholarly article by Sara Pellegrino published in December 2014

Moonlighting O-acetylserine sulfhydrylase: New functions for an old protein

scientific article

Nanoscale dynamical investigation of the hemoglobin complex with the bacterial protein IsdB: is their interaction stabilized by catch bonds?

scientific article published in 2024

New insights into allosteric mechanisms from trapping unstable protein conformations in silica gels

scholarly article

Oxygen binding to heme proteins in solution, encapsulated in silica gels, and in the crystalline state

scientific article published on January 2008

Photoinduced millisecond switching kinetics in the GFPMut2 E222Q mutant.

scientific article

Postoperative outcome of patients undergoing lung resection presenting with new-onset atrial fibrillation managed by amiodarone or diltiazem

scientific article published on 20 November 2006

Protein crystal microspectrophotometry ⬇️

scientific article published on December 22, 2010

Protein dynamics: Experimental and computational approaches

scientific article published in August 2011

Pyridoxal 5'-phosphate enzymes as targets for therapeutic agents

scientific article published on January 2007

Rational Design of a User-Friendly Aptamer/Peptide-Based Device for the Detection of Staphylococcus aureus

scientific article published on 02 September 2020

Rational Design, Synthesis, and Preliminary Structure-Activity Relationships of α-Substituted-2-Phenylcyclopropane Carboxylic Acids as Inhibitors of Salmonella typhimurium O-Acetylserine Sulfhydrylase

scientific article published on 19 February 2016

Regulation of human serine racemase activity and dynamics by halides, ATP and malonate

article

Role of histidine 148 in stability and dynamics of a highly fluorescent GFP variant

scientific article published on 26 January 2013

Role of pyridoxal 5'-phosphate in the structural stabilization of O-acetylserine sulfhydrylase.

scientific article

Role of tertiary structures on the Root effect in fish hemoglobins

scientific article

Spectroscopic and functional characterization of T state hemoglobin conformations encapsulated in silica gels

scientific article published in November 2004

Structural insight into the interaction of O-acetylserine sulfhydrylase with competitive, peptidic inhibitors by saturation transfer difference-NMR.

scientific article

Structure and Oxygen Affinity of Crystalline des-His-146β Human Hemoglobin in the T State

scientific article published in Journal of Biological Chemistry

Structure and single crystal spectroscopy of Green Fluorescent Proteins.

scientific article

Structure, mechanism, and conformational dynamics of O-acetylserine sulfhydrylase from Salmonella typhimurium: comparison of A and B isozymes

scientific article

Study of DNA binding and bending by Bacillus subtilis GabR, a PLP-dependent transcription factor

scientific article

Surface-exposed tryptophan residues are essential for O-acetylserine sulfhydrylase structure, function, and stability

scientific article

T State Hemoglobin Binds Oxygen Noncooperatively with Allosteric Effects of Protons, Inositol Hexaphosphate, and Chloride ⬇️

scientific article published in Journal of Biological Chemistry

Tertiary and Quaternary Allostery in Tetrameric Hemoglobin from Scapharca inaequivalvis

scientific article

Tertiary and quaternary effects in the allosteric regulation of animal hemoglobins ⬇️

scientific article published on March 20, 2013

The molecular pathway for the allosteric regulation of tryptophan synthase

scientific article

The multifaceted pyridoxal 5'-phosphate-dependent O-acetylserine sulfhydrylase

scientific article published on 28 April 2011

Thoracoscopic parietal pleural argon beam coagulation versus pleural abrasion in the treatment of primary spontaneous pneumothorax

scientific article published on 6 December 2005

Towards a novel haemoglobin-based oxygen carrier: Euro-PEG-Hb, physico-chemical properties, vasoactivity and renal filtration.

scientific article published on 20 March 2008

Tracking unfolding and refolding of single GFPmut2 molecules

scientific article

Trapping Hemoglobin in Rigid Matrices: Fine Tuning of Oxygen Binding Properties by Modulation of Encapsulation Protocols

scholarly article by Stefano Bruno et al published January 2007 in Artificial Cells, Blood Substitutes, and Biotechnology

Tryptophan synthase: a mine for enzymologists

scientific article

Tyrosine phenol-lyase and tryptophan indole-lyase encapsulated in wet nanoporous silica gels: Selective stabilization of tertiary conformations

scientific article published on April 2004

Unfolding of Green Fluorescent Protein mut2 in wet nanoporous silica gels.

scientific article

Unfolding of pyridoxal 5'-phosphate-dependent O-acetylserine sulfhydrylase probed by time-resolved tryptophan fluorescence.

scientific article

pH dependence of tryptophan synthase catalytic mechanism: I. The first stage, the beta-elimination reaction

scientific article published on 26 April 2004

List of works by Stefano Bettati

A Trivalent Enzymatic System for Uricolytic Therapy of HPRT Deficiency and Lesch-Nyhan Disease.

A tertiary two-state allosteric model for hemoglobin

A two-step process controls the formation of the bienzyme cysteine synthase complex

Activation of an anti-bacterial toxin by the biosynthetic enzyme CysK: mechanism of binding, interaction specificity and competition with cysteine synthase.

Allosteric communication between alpha and beta subunits of tryptophan synthase: modelling the open-closed transition of the alpha subunit

Allosteric communication of tryptophan synthase. Functional and regulatory properties of the beta S178P mutant

Allosteric mechanism of haemoglobin: rupture of salt-bridges raises the oxygen affinity of the T-structure 1 1Edited by D. Rees

Asymmetry of the active site loop conformation between subunits of glutamate-1-semialdehyde aminomutase in solution

CO Rebinding Kinetics to Myoglobin- and R-State-Hemoglobin-Doped Silica Gels in the Presence of Glycerol

Catalysis and Structure of Zebrafish Urate Oxidase Provide Insights into the Origin of Hyperuricemia in Hominoids

Catalytic competence of O-acetylserine sulfhydrylase in the crystal probed by polarized absorption microspectrophotometry

Circular dichroism spectroscopy of tertiary and quaternary conformations of human hemoglobin entrapped in wet silica gels

Combination of SAXS and Protein Painting Discloses the Three-Dimensional Organization of the Bacterial Cysteine Synthase Complex, a Potential Target for Enhancers of Antibiotic Action

Confinement and crowding effects on tryptophan synthase α2β2complex

Conformational probes of O-acetylserine sulfhydrylase: fluorescence of tryptophans 50 and 161

Cooperative Oxygen Binding toScapharca inaequivalvisHemoglobin in the Crystal

Correlation of protein functional properties in the crystal and in solution: the case study of T-state hemoglobin

Cryo-EM structures of staphylococcal IsdB bound to human hemoglobin reveal the process of heme extraction

Cyclopropane-1,2-dicarboxylic acids as new tools for the biophysical investigation of O-acetylserine sulfhydrylases by fluorimetric methods and saturation transfer difference (STD) NMR.

Design of O -Acetylserine Sulfhydrylase Inhibitors by Mimicking Nature

Diatom Allantoin Synthase Provides Structural Insights into Natural Fusion Protein Therapeutics

Dynamics of green fluorescent protein mutant2 in solution, on spin-coated glasses, and encapsulated in wet silica gels

Effect of the point mutation H148G on GFPmut2 unfolding kinetics by fluorescence spectroscopy

Engineering hemoglobin to enable homogenous PEGylation without modifying protein functionality

Environment effects on the oscillatory unfolding kinetics of GFP.

Evidence for Two Geminate Rebinding States Following Laser Photolysis of R State Hemoglobin Encapsulated in Wet Silica Gels

Evidence of discrete substates and unfolding pathways in green fluorescent protein

Evolution of allosteric models for hemoglobin

Exercise capacity assessment in patients undergoing lung resection.

Experimental basis for a new allosteric model for multisubunit proteins

Experiments on Hemoglobin in Single Crystals and Silica Gels Distinguish among Allosteric Models.

Exploring O-acetylserine sulfhydrylase-B isoenzyme from Salmonella typhimurium by fluorescence spectroscopy

Exploring the pyridoxal 5′-phosphate-dependent enzymes

Functional characterization of heme proteins encapsulated in wet nanoporous silica gels.

Geminate rebinding in R-state hemoglobin: kinetic and computational evidence for multiple hydrophobic pockets

Glutamine 89 is a key residue in the allosteric modulation of human serine racemase activity by ATP.

Haemoglobin-based oxygen carriers: research and reality towards an alternative to blood transfusions

Hemocyanin from E. californicum encapsulated in silica gels: oxygen binding and conformational states

Hemoglobin Reactivity and Regulation

Hemoglobin, an "evergreen" red protein

High and low oxygen affinity conformations of T state hemoglobin

Identification of the geometric requirements for allosteric communication between the alpha- and beta-subunits of tryptophan synthase

Identification of the structural determinants for the stability of substrate and aminoacrylate external Schiff bases in O-acetylserine sulfhydrylase-A.

Immobilization of Allantoinase for the Development of an Optical Biosensor of Oxidative Stress States

Insight into GFPmut2 pH Dependence by Single Crystal Microspectrophotometry and X-ray Crystallography

Interaction of human hemoglobin and semi-hemoglobins with the Staphylococcus aureus hemophore IsdB: a kinetic and mechanistic insight

Interaction of serine acetyltransferase with O-acetylserine sulfhydrylase active site: evidence from fluorescence spectroscopy

Iron Metabolism at the Interface between Host and Pathogen: From Nutritional Immunity to Antibacterial Development

Kinetics of acid-induced spectral changes in the GFPmut2 chromophore

Ligand reactivity and allosteric regulation of hemoglobin-based oxygen carriers.

Ligand-induced tertiary relaxations during the T-to-R quaternary transition in hemoglobin

Lung metastasis resection of adenoid cystic carcinoma of salivary glands

Modulation of Escherichia coli serine acetyltransferase catalytic activity in the cysteine synthase complex

Molecular insights into dimerization inhibition of c-Maf transcription factor

Moonlighting O-acetylserine sulfhydrylase: New functions for an old protein

Nanoscale dynamical investigation of the hemoglobin complex with the bacterial protein IsdB: is their interaction stabilized by catch bonds?

New insights into allosteric mechanisms from trapping unstable protein conformations in silica gels

Oxygen binding to heme proteins in solution, encapsulated in silica gels, and in the crystalline state

Photoinduced millisecond switching kinetics in the GFPMut2 E222Q mutant.

Postoperative outcome of patients undergoing lung resection presenting with new-onset atrial fibrillation managed by amiodarone or diltiazem

Protein crystal microspectrophotometry ⬇️

Protein dynamics: Experimental and computational approaches

Pyridoxal 5'-phosphate enzymes as targets for therapeutic agents

Rational Design of a User-Friendly Aptamer/Peptide-Based Device for the Detection of Staphylococcus aureus

Rational Design, Synthesis, and Preliminary Structure-Activity Relationships of α-Substituted-2-Phenylcyclopropane Carboxylic Acids as Inhibitors of Salmonella typhimurium O-Acetylserine Sulfhydrylase

Regulation of human serine racemase activity and dynamics by halides, ATP and malonate

Role of histidine 148 in stability and dynamics of a highly fluorescent GFP variant

Role of pyridoxal 5'-phosphate in the structural stabilization of O-acetylserine sulfhydrylase.

Role of tertiary structures on the Root effect in fish hemoglobins

Spectroscopic and functional characterization of T state hemoglobin conformations encapsulated in silica gels

Structural insight into the interaction of O-acetylserine sulfhydrylase with competitive, peptidic inhibitors by saturation transfer difference-NMR.

Structure and Oxygen Affinity of Crystalline des-His-146β Human Hemoglobin in the T State

Structure and single crystal spectroscopy of Green Fluorescent Proteins.

Structure, mechanism, and conformational dynamics of O-acetylserine sulfhydrylase from Salmonella typhimurium: comparison of A and B isozymes

Study of DNA binding and bending by Bacillus subtilis GabR, a PLP-dependent transcription factor

Surface-exposed tryptophan residues are essential for O-acetylserine sulfhydrylase structure, function, and stability

T State Hemoglobin Binds Oxygen Noncooperatively with Allosteric Effects of Protons, Inositol Hexaphosphate, and Chloride ⬇️

Tertiary and Quaternary Allostery in Tetrameric Hemoglobin from Scapharca inaequivalvis

Tertiary and quaternary effects in the allosteric regulation of animal hemoglobins ⬇️