List of works by Miguel Alcalde

A chemo-enzymatic oxidation cascade to activate C-H bonds with in situ generated H2O2

scientific article published on 13 September 2019

A modular two yeast species secretion system for the production and preparative application of unspecific peroxygenases

scientific article published in 2021

Altering the laccase functionality by in vivo assembly of mutant libraries with different mutational spectra

scientific article

Ancestral Resurrection and Directed Evolution of Fungal Mesozoic Laccases

scientific article published on 15 May 2020

Assembly of evolved ligninolytic genes in Saccharomyces cerevisiae.

scientific article

Beet sugar syrup and molasses as low-cost feedstock for the enzymatic production of fructo-oligosaccharides

scientific article published on 01 April 2006

Biocatalytic Aromaticity-Breaking Epoxidation of Naphthalene and Nucleophilic Ring-Opening Reactions

scientific article published on 12 February 2021

Bioelectrochemical oxidation of water

scientific article published on 11 April 2014

Blood tolerant laccase by directed evolution

scientific article published in February 2013

Chemical modification of lysine side chains of cyclodextrin glycosyltransferase from Thermoanaerobacter causes a shift from cyclodextrin glycosyltransferase to alpha-amylase specificity

scientific article published on 01 February 1999

Colorimetric assays for biodegradation of polycyclic aromatic hydrocarbons by fungal laccases

scientific article

Colorimetric high-throughput assay for alkene epoxidation catalyzed by cytochrome P450 BM-3 variant 139-3.

scientific article published in March 2004

Combinatorial saturation mutagenesis by in vivo overlap extension for the engineering of fungal laccases

scientific article

Combinatorial saturation mutagenesis of the Myceliophthora thermophila laccase T2 mutant: the connection between the C-terminal plug and the conserved (509)VSG(511) tripeptide

scientific article

Conversion of a carboxylesterase into a triacylglycerol lipase by a random mutation

scientific article published on 01 November 2005

Correction for Gomez-Fernandez et al., "Ancestral Resurrection and Directed Evolution of Fungal Mesozoic Laccases"

scientific article published on 18 August 2020

Directed evolution of a temperature-, peroxide- and alkaline pH-tolerant versatile peroxidase

scientific article published on 01 January 2012

Directed evolution of fungal laccases

scientific article

Directed evolution of unspecific peroxygenase from Agrocybe aegerita

scientific article

Effect of carbohydrate fatty acid esters on Streptococcus sobrinus and glucosyltransferase activity

scientific article published in April 2004

Effect of chemical modification of cyclodextrin glycosyltransferase (CGTase) from Thermoanaerobacter sp. on its activity and product selectivity

scientific article published on 01 December 1998

Engineering and Applications of fungal laccases for organic synthesis.

scientific article

Engineering platforms for directed evolution of Laccase from Pycnoporus cinnabarinus.

scientific article

Engineering the ligninolytic enzyme consortium

scientific article published on 15 January 2015

Evolving thermostability in mutant libraries of ligninolytic oxidoreductases expressed in yeast

scientific article

Expanding the Spectrum of Light-Driven Peroxygenase Reactions

scientific article published on 18 December 2018

Exploring the Role of Phenylalanine Residues in Modulating the Flexibility and Topography of the Active Site in the Peroxygenase Variant PaDa-I

scientific article published on 10 August 2020

Focused Directed Evolution of Aryl-Alcohol Oxidase in Saccharomyces cerevisiae by Using Chimeric Signal Peptides

scientific article

Functional expression of a blood tolerant laccase in Pichia pastoris.

scientific article

Functional expression of a fungal laccase in Saccharomyces cerevisiae by directed evolution.

scientific article

Functional expression of aryl-alcohol oxidase in Saccharomyces cerevisiae and Pichia pastoris by directed evolution.

scientific article published on 13 March 2018

Identification of a γ-hexachlorocyclohexane dehydrochlorinase (LinA) variant with improved expression and solubility properties

Immobilized biocatalysts: novel approaches and tools for binding enzymes to supports

scientific article published on 01 November 2011

In vitro evolution of a fungal laccase in high concentrations of organic cosolvents.

scientific article

Laboratory evolution of high-redox potential laccases.

scientific article

Laccase engineering: from rational design to directed evolution.

scientific article published on 27 December 2014

Laccases and their applications: a patent review

scientific article published on January 2008

Modification of galactose oxidase to introduce glucose 6-oxidase activity

scientific article published in August 2002

Mutagenesis Protocols in Saccharomyces cerevisiae by In Vivo Overlap Extension ⬇️

scientific article published on January 1, 2010

Mutagenic Organized Recombination Process by Homologous IN vivo Grouping (MORPHING) for directed enzyme evolution.

scientific article

New colorimetric screening assays for the directed evolution of fungal laccases to improve the conversion of plant biomass

scientific article

Novel polyphenol oxidase mined from a metagenome expression library of bovine rumen: biochemical properties, structural analysis, and phylogenetic relationships.

scientific article

Peroxygenase-Catalyzed Oxyfunctionalization Reactions Promoted by the Complete Oxidation of Methanol.

scientific article published on 26 November 2015

Plasma-Driven in Situ Production of Hydrogen Peroxide for Biocatalysis

scientific article published on 05 February 2020

Saccharomyces cerevisiae in directed evolution: An efficient tool to improve enzymes.

scientific article

Screening mutant libraries of fungal laccases in the presence of organic solvents.

scientific article

Screening β-fructofuranosidases mutant libraries to enhance the transglycosylation rates of β-(2→6) fructooligosaccharides

scientific article

Selective Activation of C-H Bonds in a Cascade Process Combining Photochemistry and Biocatalysis.

scientific article published on 10 October 2017

Self-powered wireless carbohydrate/oxygen sensitive biodevice based on radio signal transmission

scientific article

Semi-rational engineering of cellobiose dehydrogenase for improved hydrogen peroxide production.

scientific article

Shuffling the neutral drift of unspecific peroxygenase in

scientific article published on 17 July 2018

Structural insights into the substrate promiscuity of a laboratory-evolved peroxygenase

scientific article published on 16 November 2018

Succinylation of cyclodextrin glycosyltransferase from Thermoanaerobacter sp. 501 enhances its transferase activity using starch as donor

scientific article

Synthesis of 1-Naphthol by a Natural Peroxygenase Engineered by Directed Evolution

scientific article published on 17 December 2015

Synthesis of methyl alpha-D-glucooligosaccharides by entrapped dextransucrase from Leuconostoc mesenteroides B-1299.

scientific article published on 2 March 2006

Tandem-yeast expression system for engineering and producing unspecific peroxygenase.

scientific article published on 23 March 2015

The Generation of Thermostable Fungal Laccase Chimeras by SCHEMA-RASPP Structure-Guided Recombination in Vivo

scientific article published on 01 April 2019

Transformation of Polycyclic Aromatic Hydrocarbons by Laccase Is Strongly Enhanced by Phenolic Compounds Present in Soil

scientific article published on 01 April 2007

Use and improvement of microbial redox enzymes for environmental purposes

scientific article

Widening the pH activity profile of a fungal laccase by directed evolution

scientific article published on 16 April 2013

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