List of works - Margriet Ouwens

Activin A is associated with impaired myocardial glucose metabolism and left ventricular remodeling in patients with uncomplicated type 2 diabetes

scientific article

Acute hepatic steatosis in mice by blocking beta-oxidation does not reduce insulin sensitivity of very-low-density lipoprotein production

scientific article published on 7 April 2005

Adipocyte-derived factors impair insulin signaling in differentiated human vascular smooth muscle cells via the upregulation of miR-143.

scientific article

Altered myocardial substrate metabolism is associated with myocardial dysfunction in early diabetic cardiomyopathy in rats: studies using positron emission tomography.

scientific article published on 22 July 2009

Anti-inflammatory cytokines and risk of type 2 diabetes.

scientific article published on September 2013

Arsenite stimulated glucose transport in 3T3-L1 adipocytes involves both Glut4 translocation and p38 MAPK activity.

scientific article published on October 2003

Beneficial and adverse effects of testosterone on the cardiovascular system in men.

scientific article published on 24 September 2013

CD36 deficiency increases insulin sensitivity in muscle, but induces insulin resistance in the liver in mice

scientific article published on 16 August 2003

CD36 inhibition prevents lipid accumulation and contractile dysfunction in rat cardiomyocytes

scientific article

CDH13 abundance interferes with adipocyte differentiation and is a novel biomarker for adipose tissue health

scientific article published on 21 February 2018

Cardiac contractile dysfunction in insulin-resistant rats fed a high-fat diet is associated with elevated CD36-mediated fatty acid uptake and esterification

scientific article (publication date: September 2007)

Cardioprotective properties of omentin-1 in type 2 diabetes: evidence from clinical and in vitro studies.

scientific article

Cerebrospinal fluid levels of Alzheimer’s disease biomarkers in middle-aged patients with type 1 diabetes

scientific article published on 18 July 2014

Chemerin as biomarker for insulin sensitivity in males without typical characteristics of metabolic syndrome

scientific article published on 15 February 2012

Combined gene and protein expression of hormone-sensitive lipase and adipose triglyceride lipase, mitochondrial content, and adipocyte size in subcutaneous and visceral adipose tissue of morbidly obese men.

scientific article published on 14 October 2011

Diabetic cardiomyopathy in Zucker diabetic fatty rats: the forgotten right ventricle

scientific article published on 15 June 2010

Does dipeptidyl peptidase-4 inhibition prevent the diabetogenic effects of glucocorticoids in men with the metabolic syndrome? A randomized controlled trial

scientific article published on 4 February 2014

Dynamics of insulin signalling in liver during hyperinsulinemic euglycaemic clamp conditions in vivo and the effects of high-fat feeding in male mice

scientific article published in October 2007

Effect of Sfrp5 on cytokine release and insulin action in primary human adipocytes and skeletal muscle cells.

scientific article published on 21 January 2014

Effect of a 2-day very low-energy diet on skeletal muscle insulin sensitivity in obese type 2 diabetic patients on insulin therapy

scientific article published in December 2005

Effects of DPP-4 Inhibitor Linagliptin Versus Sulfonylurea Glimepiride as Add-on to Metformin on Renal Physiology in Overweight Patients With Type 2 Diabetes (RENALIS): A Randomized, Double-Blind Trial

scientific article published on 08 September 2020

Effects of adding exercise to a 16-week very low-calorie diet in obese, insulin-dependent type 2 diabetes mellitus patients.

scientific article

Endogenous interleukin-10 protects against hepatic steatosis but does not improve insulin sensitivity during high-fat feeding in mice

scientific article published on 18 May 2006

Endogenous oestradiol and cardiovascular disease in healthy men: a systematic review and meta-analysis of prospective studies.

scientific article published on 26 June 2012

Evaluation of proinflammatory cytokines and inflammation markers as biomarkers for the action of thiazolidinediones in Type 2 diabetes mellitus patients and healthy volunteers

scientific article published in October 2006

Expression, Enzyme Activity, and Subcellular Localization of Mammalian Target of Rapamycin in Insulin-Responsive Cells ⬇️

scientific article published on December 29, 1997

Functioning of oxidative phosphorylation in liver mitochondria of high-fat diet fed rats.

scientific article published on 10 November 2006

Glucagon-like peptide-1 receptor agonist treatment prevents glucocorticoid-induced glucose intolerance and islet-cell dysfunction in humans.

scientific article published on 7 January 2011

Glucocorticoid treatment impairs microvascular function in healthy men in association with its adverse effects on glucose metabolism and blood pressure: a randomised controlled trial

scientific article published on 11 August 2013

Glucose Intolerance and the Amount of Visceral Adipose Tissue Contribute to an Increase in Circulating Triglyceride Concentrations in Caucasian Obese Females ⬇️

scientific article published on September 28, 2012

Heat shock protein 60 as a mediator of adipose tissue inflammation and insulin resistance.

scientific article published on 7 February 2012

Hepatic VLDL production in ob/ob mice is not stimulated by massive de novo lipogenesis but is less sensitive to the suppressive effects of insulin

scientific article

High levels of dietary stearate promote adiposity and deteriorate hepatic insulin sensitivity

scientific article

Hyperosmotic stress activates the insulin receptor in CHO cells

scientific article published in August 2001

IRS-4 mediated mitogenic signalling by insulin and growth hormone in LB cells, a murine T-cell lymphoma devoid of IGF-I receptors

scientific article published in April 2003

Identification of novel adipokines differential regulated in C57BL/Ks and C57BL/6.

scientific article published on 16 October 2014

Increased Glycolysis and Higher Lactate Production in Hyperglycemic Myotubes

scientific article published on 18 September 2019

Increased hepatic insulin sensitivity together with decreased hepatic triglyceride stores in hormone-sensitive lipase-deficient mice

scientific article

Increased triacylglycerol - Fatty acid substrate cycling in human skeletal muscle cells exposed to eicosapentaenoic acid

scientific article published in PLoS ONE

Involvement of atypical protein kinase C in the regulation of cardiac glucose and long-chain fatty acid uptake

scientific article published on 11 September 2012

Knockdown of PRAS40 inhibits insulin action via proteasome-mediated degradation of IRS1 in primary human skeletal muscle cells

scientific article published on 05 March 2013

Lessons that can be learned from patients with diabetogenic mutations in mitochondrial DNA: implications for common type 2 diabetes.

scientific article

Loss of 50% of excess weight using a very low energy diet improves insulin-stimulated glucose disposal and skeletal muscle insulin signalling in obese insulin-treated type 2 diabetic patients

scientific article

MIF deficiency reduces chronic inflammation in white adipose tissue and impairs the development of insulin resistance, glucose intolerance, and associated atherosclerotic disease

scientific article published on 28 May 2009

Mammalian target of rapamycin is a direct target for protein kinase B: identification of a convergence point for opposing effects of insulin and amino-acid deficiency on protein translation.

scientific article published in December 1999

Modulation of insulin-stimulated glycogen synthesis by Src Homology Phosphatase 2.

scientific article published on April 2001

Molecular mechanisms of contraction-regulated cardiac glucose transport.

scientific article

Myocardial insulin action and the contribution of insulin resistance to the pathogenesis of diabetic cardiomyopathy.

scientific article published on April 2007

Novel insights into glucocorticoid-mediated diabetogenic effects: towards expansion of therapeutic options?

scientific article published on February 2009

Over-expression of PRAS40 enhances insulin sensitivity in skeletal muscle.

scientific article published on 27 February 2014

PRAS40: target or modulator of mTORC1 signalling and insulin action?

scientific article published on October 2009

Permissive action of protein kinase C-zeta in insulin-induced CD36- and GLUT4 translocation in cardiac myocytes

scientific journal article

Physiological Disturbance in Fatty Liver Energy Metabolism Converges on IGFBP2 Abundance and Regulation in Mice and Men

scientific article published on 10 June 2020

Prednisolone induces the Wnt signalling pathway in 3T3-L1 adipocytes.

scientific article published on 19 March 2013

Proline-rich Akt substrate of 40-kDa contains a nuclear export signal.

scientific article published on 24 May 2013

Regulation of sarcolemmal transport of substrates in the healthy and diseased heart.

scientific article published on December 2006

Role of PRAS40 in Akt and mTOR signaling in health and disease.

scientific article published on 21 February 2012

SREBP-1c expression in Schwann cells is affected by diabetes and nutritional status.

scientific article published on May 2007

Secretome profiling of primary human skeletal muscle cells

scientific article published on 27 August 2013

Secretory products from epicardial adipose tissue of patients with type 2 diabetes mellitus induce cardiomyocyte dysfunction

scientific article published on 12 October 2012

Secretory products of guinea pig epicardial fat induce insulin resistance and impair primary adult rat cardiomyocyte function

scientific article

Sex steroids affect triglyceride handling, glucose-dependent insulinotropic polypeptide, and insulin sensitivity: a 1-week randomized clinical trial in healthy young men.

scientific article published on 18 May 2010

Sfrp5 increases glucose-stimulated insulin secretion in the rat pancreatic beta cell line INS-1E

scientific article published on 28 March 2019

Short-term increase of plasma free fatty acids does not interfere with intrinsic mitochondrial function in healthy young men

Skin fibroblasts of children with idiopathic short stature show an increased mitogenic response to IGF-I and secrete more IGFBP-3.

scientific article

Soluble CD14 inhibits contractile function and insulin action in primary adult rat cardiomyocytes.

scientific article published on 2 November 2016

Subcellular trafficking of the substrate transporters GLUT4 and CD36 in cardiomyocytes ⬇️

scientific article published on May 6, 2011

Sustained activation of the mammalian target of rapamycin nutrient sensing pathway is associated with hepatic insulin resistance, but not with steatosis, in mice

scientific article published on 28 September 2006

The dopamine receptor D2 agonist bromocriptine inhibits glucose-stimulated insulin secretion by direct activation of the alpha2-adrenergic receptors in beta cells.

scientific article

The insulin sensitizing effect of topiramate involves KATP channel activation in the central nervous system

scientific article published on October 2013

The novel adipokine WISP1 associates with insulin resistance and impairs insulin action in human myotubes and mouse hepatocytes.

scientific article

The nuclear appearance of ERK1/2 and p38 determines the sequential induction of ATF2-Thr71 and ATF2-Thr69 phosphorylation by serum in JNK-deficient cells.

scientific article published on 30 July 2009

The role of c-Jun N-terminal kinase, p38, and extracellular signal-regulated kinase in insulin-induced Thr69 and Thr71 phosphorylation of activating transcription factor 2.

scientific article published on 6 April 2006

Tissue-specific differences in the development of insulin resistance in a mouse model for type 1 diabetes

scientific article

Two Naturally Occurring Insulin Receptor Tyrosine Kinase Domain Mutants Provide Evidence That Phosphoinositide 3-Kinase Activation Alone Is Not Sufficient for the Mediation of Insulin's Metabolic and Mitogenic Effects ⬇️

scientific article published in Journal of Biological Chemistry

VEGF in the crosstalk between human adipocytes and smooth muscle cells: depot-specific release from visceral and perivascular adipose tissue

scientific article published on 09 July 2013

List of works by Margriet Ouwens

Activin A is associated with impaired myocardial glucose metabolism and left ventricular remodeling in patients with uncomplicated type 2 diabetes

Acute hepatic steatosis in mice by blocking beta-oxidation does not reduce insulin sensitivity of very-low-density lipoprotein production

Adipocyte-derived factors impair insulin signaling in differentiated human vascular smooth muscle cells via the upregulation of miR-143.

Altered myocardial substrate metabolism is associated with myocardial dysfunction in early diabetic cardiomyopathy in rats: studies using positron emission tomography.

Anti-inflammatory cytokines and risk of type 2 diabetes.

Arsenite stimulated glucose transport in 3T3-L1 adipocytes involves both Glut4 translocation and p38 MAPK activity.

Beneficial and adverse effects of testosterone on the cardiovascular system in men.

CD36 deficiency increases insulin sensitivity in muscle, but induces insulin resistance in the liver in mice

CD36 inhibition prevents lipid accumulation and contractile dysfunction in rat cardiomyocytes

CDH13 abundance interferes with adipocyte differentiation and is a novel biomarker for adipose tissue health

Cardiac contractile dysfunction in insulin-resistant rats fed a high-fat diet is associated with elevated CD36-mediated fatty acid uptake and esterification

Cardioprotective properties of omentin-1 in type 2 diabetes: evidence from clinical and in vitro studies.

Cerebrospinal fluid levels of Alzheimer’s disease biomarkers in middle-aged patients with type 1 diabetes

Chemerin as biomarker for insulin sensitivity in males without typical characteristics of metabolic syndrome

Combined gene and protein expression of hormone-sensitive lipase and adipose triglyceride lipase, mitochondrial content, and adipocyte size in subcutaneous and visceral adipose tissue of morbidly obese men.

Diabetic cardiomyopathy in Zucker diabetic fatty rats: the forgotten right ventricle

Does dipeptidyl peptidase-4 inhibition prevent the diabetogenic effects of glucocorticoids in men with the metabolic syndrome? A randomized controlled trial

Dynamics of insulin signalling in liver during hyperinsulinemic euglycaemic clamp conditions in vivo and the effects of high-fat feeding in male mice

Effect of Sfrp5 on cytokine release and insulin action in primary human adipocytes and skeletal muscle cells.

Effect of a 2-day very low-energy diet on skeletal muscle insulin sensitivity in obese type 2 diabetic patients on insulin therapy

Effects of DPP-4 Inhibitor Linagliptin Versus Sulfonylurea Glimepiride as Add-on to Metformin on Renal Physiology in Overweight Patients With Type 2 Diabetes (RENALIS): A Randomized, Double-Blind Trial

Effects of adding exercise to a 16-week very low-calorie diet in obese, insulin-dependent type 2 diabetes mellitus patients.

Endogenous interleukin-10 protects against hepatic steatosis but does not improve insulin sensitivity during high-fat feeding in mice

Endogenous oestradiol and cardiovascular disease in healthy men: a systematic review and meta-analysis of prospective studies.

Evaluation of proinflammatory cytokines and inflammation markers as biomarkers for the action of thiazolidinediones in Type 2 diabetes mellitus patients and healthy volunteers

Expression, Enzyme Activity, and Subcellular Localization of Mammalian Target of Rapamycin in Insulin-Responsive Cells ⬇️

Functioning of oxidative phosphorylation in liver mitochondria of high-fat diet fed rats.

Glucagon-like peptide-1 receptor agonist treatment prevents glucocorticoid-induced glucose intolerance and islet-cell dysfunction in humans.

Glucocorticoid treatment impairs microvascular function in healthy men in association with its adverse effects on glucose metabolism and blood pressure: a randomised controlled trial

Glucose Intolerance and the Amount of Visceral Adipose Tissue Contribute to an Increase in Circulating Triglyceride Concentrations in Caucasian Obese Females ⬇️

Heat shock protein 60 as a mediator of adipose tissue inflammation and insulin resistance.

Hepatic VLDL production in ob/ob mice is not stimulated by massive de novo lipogenesis but is less sensitive to the suppressive effects of insulin

High levels of dietary stearate promote adiposity and deteriorate hepatic insulin sensitivity

Hyperosmotic stress activates the insulin receptor in CHO cells

IRS-4 mediated mitogenic signalling by insulin and growth hormone in LB cells, a murine T-cell lymphoma devoid of IGF-I receptors

Identification of novel adipokines differential regulated in C57BL/Ks and C57BL/6.

Increased Glycolysis and Higher Lactate Production in Hyperglycemic Myotubes

Increased hepatic insulin sensitivity together with decreased hepatic triglyceride stores in hormone-sensitive lipase-deficient mice

Increased triacylglycerol - Fatty acid substrate cycling in human skeletal muscle cells exposed to eicosapentaenoic acid

Involvement of atypical protein kinase C in the regulation of cardiac glucose and long-chain fatty acid uptake

Knockdown of PRAS40 inhibits insulin action via proteasome-mediated degradation of IRS1 in primary human skeletal muscle cells

Lessons that can be learned from patients with diabetogenic mutations in mitochondrial DNA: implications for common type 2 diabetes.

Loss of 50% of excess weight using a very low energy diet improves insulin-stimulated glucose disposal and skeletal muscle insulin signalling in obese insulin-treated type 2 diabetic patients

MIF deficiency reduces chronic inflammation in white adipose tissue and impairs the development of insulin resistance, glucose intolerance, and associated atherosclerotic disease

Mammalian target of rapamycin is a direct target for protein kinase B: identification of a convergence point for opposing effects of insulin and amino-acid deficiency on protein translation.

Modulation of insulin-stimulated glycogen synthesis by Src Homology Phosphatase 2.

Molecular mechanisms of contraction-regulated cardiac glucose transport.

Myocardial insulin action and the contribution of insulin resistance to the pathogenesis of diabetic cardiomyopathy.

Novel insights into glucocorticoid-mediated diabetogenic effects: towards expansion of therapeutic options?

Over-expression of PRAS40 enhances insulin sensitivity in skeletal muscle.

PRAS40: target or modulator of mTORC1 signalling and insulin action?

Permissive action of protein kinase C-zeta in insulin-induced CD36- and GLUT4 translocation in cardiac myocytes

Physiological Disturbance in Fatty Liver Energy Metabolism Converges on IGFBP2 Abundance and Regulation in Mice and Men

Prednisolone induces the Wnt signalling pathway in 3T3-L1 adipocytes.

Proline-rich Akt substrate of 40-kDa contains a nuclear export signal.

Regulation of sarcolemmal transport of substrates in the healthy and diseased heart.

Role of PRAS40 in Akt and mTOR signaling in health and disease.

SREBP-1c expression in Schwann cells is affected by diabetes and nutritional status.

Secretome profiling of primary human skeletal muscle cells

Secretory products from epicardial adipose tissue of patients with type 2 diabetes mellitus induce cardiomyocyte dysfunction

Secretory products of guinea pig epicardial fat induce insulin resistance and impair primary adult rat cardiomyocyte function

Sex steroids affect triglyceride handling, glucose-dependent insulinotropic polypeptide, and insulin sensitivity: a 1-week randomized clinical trial in healthy young men.

Sfrp5 increases glucose-stimulated insulin secretion in the rat pancreatic beta cell line INS-1E

Short-term increase of plasma free fatty acids does not interfere with intrinsic mitochondrial function in healthy young men

Skin fibroblasts of children with idiopathic short stature show an increased mitogenic response to IGF-I and secrete more IGFBP-3.

Soluble CD14 inhibits contractile function and insulin action in primary adult rat cardiomyocytes.

Subcellular trafficking of the substrate transporters GLUT4 and CD36 in cardiomyocytes ⬇️

Sustained activation of the mammalian target of rapamycin nutrient sensing pathway is associated with hepatic insulin resistance, but not with steatosis, in mice

The dopamine receptor D2 agonist bromocriptine inhibits glucose-stimulated insulin secretion by direct activation of the alpha2-adrenergic receptors in beta cells.

The insulin sensitizing effect of topiramate involves KATP channel activation in the central nervous system

The novel adipokine WISP1 associates with insulin resistance and impairs insulin action in human myotubes and mouse hepatocytes.

The nuclear appearance of ERK1/2 and p38 determines the sequential induction of ATF2-Thr71 and ATF2-Thr69 phosphorylation by serum in JNK-deficient cells.

The role of c-Jun N-terminal kinase, p38, and extracellular signal-regulated kinase in insulin-induced Thr69 and Thr71 phosphorylation of activating transcription factor 2.

Tissue-specific differences in the development of insulin resistance in a mouse model for type 1 diabetes

Two Naturally Occurring Insulin Receptor Tyrosine Kinase Domain Mutants Provide Evidence That Phosphoinositide 3-Kinase Activation Alone Is Not Sufficient for the Mediation of Insulin's Metabolic and Mitogenic Effects ⬇️

VEGF in the crosstalk between human adipocytes and smooth muscle cells: depot-specific release from visceral and perivascular adipose tissue