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List of works by Elizabeth Howell

"Catch 222," the effects of symmetry on ligand binding and catalysis in R67 dihydrofolate reductase as determined by mutations at Tyr-69.

scientific article

A Structural Basis for Biguanide Activity.

scientific article

A balancing act between net uptake of water during dihydrofolate binding and net release of water upon NADPH binding in R67 dihydrofolate reductase

scientific article

A glutamine 67--> histidine mutation in homotetrameric R67 dihydrofolate reductase results in four mutations per single active site pore and causes substantial substrate and cofactor inhibition

scientific article published on 01 December 1997

A multilaboratory comparison of calibration accuracy and the performance of external references in analytical ultracentrifugation

scientific article published on 21 May 2015

A plasmid-encoded dihydrofolate reductase from trimethoprim-resistant bacteria has a novel D2-symmetric active site

scientific article

A preference for edgewise interactions between aromatic rings and carboxylate anions: the biological relevance of anion-quadrupole interactions.

scientific article

A second-site mutation at phenylalanine-137 that increases catalytic efficiency in the mutant aspartate-27----serine Escherichia coli dihydrofolate reductase.

scientific article published in September 1990

A survey of aspartate-phenylalanine and glutamate-phenylalanine interactions in the protein data bank: searching for anion-π pairs

scientific article

An engineered disulfide bond in dihydrofolate reductase.

scientific article

Analysis of hydride transfer and cofactor fluorescence decay in mutants of dihydrofolate reductase: possible evidence for participation of enzyme molecular motions in catalysis

scientific article published in December 1991

Anomerization of glucose 6-phosphate: Catalysis by phosphoglucose isomerase ⬇️

scientific article published on January 1, 1979

Artificial duplication of the R67 dihydrofolate reductase gene to create protein asymmetry. Effects on protein activity and folding.

scientific article published in October 1993

Aspects of Weak Interactions between Folate and Glycine Betaine

scientific article

Bisubstrate kinetics using SAS computer software.

scientific article published in February 2002

Breaking symmetry: mutations engineered into R67 dihydrofolate reductase, a D2 symmetric homotetramer possessing a single active site pore

scientific article

Calorimetric studies of ligand binding in R67 dihydrofolate reductase

scientific article

Coated Tube Enzyme Immunoassay: Factors Affecting Sensitivity and Effects of Reversible Protein Binding to Polystyrene

scientific article published on 01 January 1981

Comparative inactivation and inhibition of the anomerase and isomerase activities of phosphoglucose isomerase

scientific article published in 1981

Construction of a dihydrofolate reductase-deficient mutant of Escherichia coli by gene replacement

scientific article

Construction of a synthetic gene for an R-plasmid-encoded dihydrofolate reductase and studies on the role of the N-terminus in the protein.

scientific article

Construction of an altered proton donation mechanism in Escherichia coli dihydrofolate reductase

scientific article

Crowders Steal Dihydrofolate Reductase Ligands through Quinary Interactions

scientific article published on 18 February 2019

Crystal Structure of a Type II Dihydrofolate Reductase Catalytic Ternary Complex †

scientific article

Defining the binding site of homotetrameric R67 dihydrofolate reductase and correlating binding enthalpy with catalysis

scientific article

Determining anion-quadrupole interactions among protein, DNA, and ligand molecules

Dihydrofolate reductase from Escherichia coli: probing the role of aspartate-27 and phenylalanine-137 in enzyme conformation and the binding of NADPH

scientific article published on 01 September 1990

Directed mutagenesis of dihydrofolate reductase

scientific article published in Science

Does R67 dihydrofolate reductase possess a proton donor?

scientific article

Dynamics of trimethoprim bound to dihydrofolate reductase--a deuterium NMR study.

scientific article

Effects of high hydrostatic pressure or hydrophobic modification on thermal stability of xanthine oxidase

scientific article published on 28 April 2017

Effects of insertions and deletions in a beta-bulge region of Escherichia coli dihydrofolate reductase.

scientific article

Effects of single-tryptophan mutations on R67 dihydrofolate reductase.

scientific article

Effects of temperature and viscosity on R67 dihydrofolate reductase catalysis

scientific article

Equilibrium Folding Studies of Tetrameric R67 Dihydrofolate Reductase

scientific article published on 01 April 1994

Further studies on the role of water in R67 dihydrofolate reductase

scientific article

Glucose oxidase stabilization against thermal inactivation using high hydrostatic pressure and hydrophobic modification

scientific article

Highly Dynamic Anion-Quadrupole Networks in Proteins

scientific article

How do mutations at phenylalanine-153 and isoleucine-155 partially suppress the effects of the aspartate-27-->serine mutation in Escherichia coli dihydrofolate reductase?

scientific article

Intact protein analysis for site-directed mutagenesis overexpression products: plasmid-encoded R67 dihydrofolate reductase

scientific article

Interligand Overhauser effects in type II dihydrofolate reductase

scientific article published in April 2001

Investigation of osmolyte effects on FolM: comparison with other dihydrofolate reductases.

scientific article

Investigation of the functional role of tryptophan-22 in Escherichia coli dihydrofolate reductase by site-directed mutagenesis

scientific article

Isothermal titration calorimetry for measuring macromolecule-ligand affinity

scientific article published on 7 September 2011

Liver aldolase anomeric specificity

scientific article published on 01 June 1980

Long-range structural effects in a second-site revertant of a mutant dihydrofolate reductase

scientific article

Mechanistic studies of R67 dihydrofolate reductase. Effects of pH and an H62C mutation.

scientific article

Modulating Enzyme Activity by Altering Protein Dynamics with Solvent

scientific article published on 06 July 2018

NMR studies of the interaction of a type II dihydrofolate reductase with pyridine nucleotides reveal unexpected phosphatase and reductase activity

scientific article published in September 2003

Neutron diffraction studies of Escherichia coli dihydrofolate reductase complexed with methotrexate

scholarly article

Nonlinear fitting of bisubstrate enzyme kinetic models using SAS computer software: application to R67 dihydrofolate reductase

scientific article published in November 2004

Nuclear magnetic resonance study of the state of protonation of inhibitors bound to mutant dihydrofolate reductase lacking the active-site carboxyl

article

One site fits both: a model for the ternary complex of folate + NADPH in R67 dihydrofolate reductase, a D2 symmetric enzyme

scientific article published in November 2001

Phosphomannose isomerase. Isomerization of the predicted beta-D-fructose 6-phosphate

scientific article published in July 1978

Preliminary neutron diffraction studies of Escherichia coli dihydrofolate reductase bound to the anticancer drug methotrexate

scientific article published on 20 April 2005

R67, the other dihydrofolate reductase: rational design of an alternate active site configuration

scientific article

Radical redesign of a tandem array of four R67 dihydrofolate reductase genes yields a functional, folded protein possessing 45 substitutions

scientific article published on August 2010

Redesigning the Quaternary Structure of R67 Dihydrofolate Reductase

scientific article published in Journal of Biological Chemistry

Role of Lys-32 residues in R67 dihydrofolate reductase probed by asymmetric mutations

scientific article

Role of S65, Q67, I68, and Y69 residues in homotetrameric R67 dihydrofolate reductase

scientific article

Role of aspartate 27 in the binding of methotrexate to dihydrofolate reductase from Escherichia coli.

scientific article published in July 1988

Role of ionic interactions in ligand binding and catalysis of R67 dihydrofolate reductase

scientific article published in September 2003

STAAR: Statistical analysis of aromatic rings

article

Searching sequence space: two different approaches to dihydrofolate reductase catalysis

scientific article

Small Angle Neutron Scattering Studies of R67 Dihydrofolate Reductase, a Tetrameric Protein with Intrinsically Disordered N-Termini

scientific article published on 11 October 2017

Structure and function of alternative proton-relay mutants of dihydrofolate reductase

scientific article

Tales of Dihydrofolate Binding to R67 Dihydrofolate Reductase

scientific article published on 04 December 2015

The effect of electrostatic shielding on H tunneling in R67 dihydrofolate reductase

scientific article published on November 2009

The tail wagging the dog: insights into catalysis in R67 dihydrofolate reductase

scientific article

Thermodynamics and solvent effects on substrate and cofactor binding in Escherichia coli chromosomal dihydrofolate reductase.

scientific article

Thermodynamics and solvent linkage of macromolecule-ligand interactions

scientific article

Titration of Folate Pathway Enzymes

scientific article published in 2018

Titration of histidine 62 in R67 dihydrofolate reductase is linked to a tetramer<-->two-dimer equilibrium.

scientific article

Toward resolving the catalytic mechanism of dihydrofolate reductase using neutron and ultrahigh-resolution X-ray crystallography

scientific article

Tuning of the H-transfer coordinate in primitive versus well-evolved enzymes

scientific article published on May 2008

Vibrational structure of dihydrofolate bound to R67 dihydrofolate reductase

scientific article published on 25 October 2001

Weak Interactions between Folate and Osmolytes in Solution

scientific article published on 09 March 2012

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