List of works - Kathleen L Berkner

A Heterochromatin Domain Forms Gradually at a New Telomere and Is Dynamic at Stable Telomeres

scientific article published on 16 July 2018

A map of the sites in the polyoma genome cleaved by endonuclease Alul

scientific article published on January 1979

A new model for vitamin K-dependent carboxylation: the catalytic base that deprotonates vitamin K hydroquinone is not Cys but an activated amine

scholarly article

Abundant expression of polyomavirus middle T antigen and dihydrofolate reductase in an adenovirus recombinant.

scientific article published on April 1987

An assay for the rates of cleavage of specific sites in DNA by restriction endonucleases: its use to study the cleavage of phage lambda DNA by EcoRI and phage P22 DNA containing thymine or 5-bromouracil by HindIII.

scientific article published in March 1983

Brønsted analysis reveals Lys218 as the carboxylase active site base that deprotonates vitamin K hydroquinone to initiate vitamin K-dependent protein carboxylation

scientific article

Carboxylase overexpression effects full carboxylation but poor release and secretion of factor IX: implications for the release of vitamin K-dependent proteins

scientific article published on December 2002

Characterization of middle T antigen expressed by using an adenovirus expression system.

scientific article published on April 1987

Compound heterozygosity of novel missense mutations in the gamma-glutamyl-carboxylase gene causes hereditary combined vitamin K-dependent coagulation factor deficiency

scientific article published on 23 May 2006

Development of adenovirus vectors for the expression of heterologous genes

scientific article published on July 1988

EcoRI cleavage and methylation of DNAs containing modified pyrimidines in the recogintion sequence.

scientific article published in May 1977

Effect of the tripartite leader on synthesis of a non-viral protein in an adenovirus 5 recombinant.

scientific article

Expression of active human factor IX in transfected cells

scientific article published in July 1985

Expression of dihydrofolate reductase, and of the adjacent EIb region, in an Ad5-dihydrofolate reductase recombinant virus.

scientific article

Expression of heterologous sequences in adenoviral vectors

scientific article published in January 1992

Expression of human pancreatic polypeptide precursors from a dicistronic mRNA in mammalian cells

scientific article

Expression of recombinant vitamin K-dependent proteins in mammalian cells: factors IX and VII.

scientific article published on January 1993

Generation of adenovirus by transfection of plasmids

scientific article published on September 1983

Genomic cloning, characterization, and multilineage growth-promoting activity of human granulocyte-macrophage colony-stimulating factor.

scientific article published on May 1986

Human erythropoietin gene: high level expression in stably transfected mammalian cells and chromosome localization

scientific article

Identification of sequences within the gamma-carboxylase that represent a novel contact site with vitamin K-dependent proteins and that are required for activity

scientific article

Identification of the vitamin K-dependent carboxylase active site: Cys-99 and Cys-450 are required for both epoxidation and carboxylation

scientific article

Inhibitors of 2-ketoglutarate-dependent dioxygenases block aspartyl beta-hydroxylation of recombinant human factor IX in several mammalian expression systems

scientific article published in Journal of Biological Chemistry

Initiation of the extrinsic pathway of blood coagulation: evidence for the tissue factor dependent autoactivation of human coagulation factor VII.

scientific article published on November 1991

Insight into the coupling mechanism of the vitamin K-dependent carboxylase: mutation of histidine 160 disrupts glutamic acid carbanion formation and efficient coupling of vitamin K epoxidation to glutamic acid carboxylation

scientific article published on 22 August 2008

Isolation and Expression of cDNAs Encoding Human Factor VII

scientific article published on 01 January 1986

Isolation of the human gamma-carboxylase and a gamma-carboxylase-associated protein from factor IX-expressing mammalian cells.

scientific article published on June 1996

Mec1p associates with functionally compromised telomeres

scientific article

Methylation of γ-carboxylated Glu (Gla) allows detection by liquid chromatography-mass spectrometry and the identification of Gla residues in the γ-glutamyl carboxylase.

scientific article published on 10 May 2013

Mutations in the GGCX and ABCC6 genes in a family with pseudoxanthoma elasticum-like phenotypes

scientific article

Nonhomologous End-Joining with Minimal Sequence Loss Is Promoted by the Mre11-Rad50-Nbs1-Ctp1 Complex in Schizosaccharomyces pombe

scientific article published on 14 March 2017

Novel insight into the mechanism of the vitamin K oxidoreductase (VKOR): electron relay through Cys43 and Cys51 reduces VKOR to allow vitamin K reduction and facilitation of vitamin K-dependent protein carboxylation

scientific article

Overmethylation of DNAs by the EcoRI methylase

scientific article published on February 1978

Polynucleotide kinase exchange as an assay for class II restriction endonucleases.

scientific article published in January 1980

Polynucleotide kinase exchange reaction: quantitave assay for restriction endonuclease-generated 5'-phosphoroyl termini in DNA.

scientific article published on May 1977

Propeptide of human protein C is necessary for .gamma.-carboxylation

scientific article published on 01 November 1987

Purification and identification of bovine liver gamma-carboxylase

scientific article published on July 1992

Purification of vitamin K-dependent carboxylase from cultured cells

scientific article published on January 1997

Quantitation of the various termini generated by type II restriction endonucleases using the polynucleotide kinase exchange reaction

scientific article published on April 1979

Sir3p phosphorylation by the Slt2p pathway effects redistribution of silencing function and shortened lifespan.

scientific article published on 17 March 2003

Structure of the Syrian hamster ribosomal DNA repeat and identification of homologous and nonhomologous regions shared by human and hamster ribosomal DNAs

scientific article published in Journal of Biological Chemistry

Synthesis, purification, and characterization of an Arg152 .fwdarw. Glu site-directed mutant of recombinant human blood clotting factor VII

scientific article published on 01 April 1990

Tel1p Preferentially Associates with Short Telomeres to Stimulate Their Elongation

scientific article published on 01 September 2007

Tethered processivity of the vitamin K-dependent carboxylase: factor IX is efficiently modified in a mechanism which distinguishes Gla's from Glu's and which accounts for comprehensive carboxylation in vivo

scientific journal article

The effects of substituted pyrimidines in DNAs on cleavage by sequence-specific endonucleases

scientific article published on April 1979

The physiology of vitamin K nutriture and vitamin K-dependent protein function in atherosclerosis.

scientific article

The vitamin K oxidoreductase is a multimer that efficiently reduces vitamin K epoxide to hydroquinone to allow vitamin K-dependent protein carboxylation

scientific journal article

The vitamin K-dependent carboxylase

scientific article (publication date: August 2000)

The vitamin K-dependent carboxylase

scientific article

The vitamin K-dependent carboxylase generates γ-carboxylated glutamates by using CO2 to facilitate glutamate deprotonation in a concerted mechanism that drives catalysis.

scientific article published on 6 September 2011

The vitamin K-dependent carboxylase has been acquired by Leptospira pathogens and shows altered activity that suggests a role other than protein carboxylation

scientific article published on August 2005

VKOR paralog VKORC1L1 supports vitamin K-dependent protein carboxylation in vivo

scientific article published on 11 January 2018

Vitamin K oxygenation, glutamate carboxylation, and processivity: defining the three critical facets of catalysis by the vitamin K-dependent carboxylase

scientific article published on March 2012

Vitamin K-dependent carboxylation

scientific article (publication date: 2008)

Vitamin K-dependent carboxylation of the carboxylase

scientific article published on January 1998

Warfarin alters vitamin K metabolism: a surprising mechanism of VKORC1 uncoupling necessitates an additional reductase

scientific article published on 28 March 2018

r-VKORC1 expression in factor IX BHK cells increases the extent of factor IX carboxylation but is limited by saturation of another carboxylation component or by a shift in the rate-limiting step.

scientific article published on May 2006

List of works by Kathleen L Berkner

A Heterochromatin Domain Forms Gradually at a New Telomere and Is Dynamic at Stable Telomeres

A map of the sites in the polyoma genome cleaved by endonuclease Alul

A new model for vitamin K-dependent carboxylation: the catalytic base that deprotonates vitamin K hydroquinone is not Cys but an activated amine

Abundant expression of polyomavirus middle T antigen and dihydrofolate reductase in an adenovirus recombinant.

An assay for the rates of cleavage of specific sites in DNA by restriction endonucleases: its use to study the cleavage of phage lambda DNA by EcoRI and phage P22 DNA containing thymine or 5-bromouracil by HindIII.

Brønsted analysis reveals Lys218 as the carboxylase active site base that deprotonates vitamin K hydroquinone to initiate vitamin K-dependent protein carboxylation

Carboxylase overexpression effects full carboxylation but poor release and secretion of factor IX: implications for the release of vitamin K-dependent proteins

Characterization of middle T antigen expressed by using an adenovirus expression system.

Compound heterozygosity of novel missense mutations in the gamma-glutamyl-carboxylase gene causes hereditary combined vitamin K-dependent coagulation factor deficiency

Development of adenovirus vectors for the expression of heterologous genes

EcoRI cleavage and methylation of DNAs containing modified pyrimidines in the recogintion sequence.

Effect of the tripartite leader on synthesis of a non-viral protein in an adenovirus 5 recombinant.

Expression of active human factor IX in transfected cells

Expression of dihydrofolate reductase, and of the adjacent EIb region, in an Ad5-dihydrofolate reductase recombinant virus.

Expression of heterologous sequences in adenoviral vectors

Expression of human pancreatic polypeptide precursors from a dicistronic mRNA in mammalian cells

Expression of recombinant vitamin K-dependent proteins in mammalian cells: factors IX and VII.

Generation of adenovirus by transfection of plasmids

Genomic cloning, characterization, and multilineage growth-promoting activity of human granulocyte-macrophage colony-stimulating factor.

Human erythropoietin gene: high level expression in stably transfected mammalian cells and chromosome localization

Identification of sequences within the gamma-carboxylase that represent a novel contact site with vitamin K-dependent proteins and that are required for activity

Identification of the vitamin K-dependent carboxylase active site: Cys-99 and Cys-450 are required for both epoxidation and carboxylation

Inhibitors of 2-ketoglutarate-dependent dioxygenases block aspartyl beta-hydroxylation of recombinant human factor IX in several mammalian expression systems

Initiation of the extrinsic pathway of blood coagulation: evidence for the tissue factor dependent autoactivation of human coagulation factor VII.

Insight into the coupling mechanism of the vitamin K-dependent carboxylase: mutation of histidine 160 disrupts glutamic acid carbanion formation and efficient coupling of vitamin K epoxidation to glutamic acid carboxylation

Isolation and Expression of cDNAs Encoding Human Factor VII

Isolation of the human gamma-carboxylase and a gamma-carboxylase-associated protein from factor IX-expressing mammalian cells.

Mec1p associates with functionally compromised telomeres

Methylation of γ-carboxylated Glu (Gla) allows detection by liquid chromatography-mass spectrometry and the identification of Gla residues in the γ-glutamyl carboxylase.

Mutations in the GGCX and ABCC6 genes in a family with pseudoxanthoma elasticum-like phenotypes

Nonhomologous End-Joining with Minimal Sequence Loss Is Promoted by the Mre11-Rad50-Nbs1-Ctp1 Complex in Schizosaccharomyces pombe

Novel insight into the mechanism of the vitamin K oxidoreductase (VKOR): electron relay through Cys43 and Cys51 reduces VKOR to allow vitamin K reduction and facilitation of vitamin K-dependent protein carboxylation

Overmethylation of DNAs by the EcoRI methylase

Polynucleotide kinase exchange as an assay for class II restriction endonucleases.

Polynucleotide kinase exchange reaction: quantitave assay for restriction endonuclease-generated 5'-phosphoroyl termini in DNA.

Propeptide of human protein C is necessary for .gamma.-carboxylation

Purification and identification of bovine liver gamma-carboxylase

Purification of vitamin K-dependent carboxylase from cultured cells

Quantitation of the various termini generated by type II restriction endonucleases using the polynucleotide kinase exchange reaction

Sir3p phosphorylation by the Slt2p pathway effects redistribution of silencing function and shortened lifespan.

Structure of the Syrian hamster ribosomal DNA repeat and identification of homologous and nonhomologous regions shared by human and hamster ribosomal DNAs

Synthesis, purification, and characterization of an Arg152 .fwdarw. Glu site-directed mutant of recombinant human blood clotting factor VII

Tel1p Preferentially Associates with Short Telomeres to Stimulate Their Elongation

Tethered processivity of the vitamin K-dependent carboxylase: factor IX is efficiently modified in a mechanism which distinguishes Gla's from Glu's and which accounts for comprehensive carboxylation in vivo

The effects of substituted pyrimidines in DNAs on cleavage by sequence-specific endonucleases

The physiology of vitamin K nutriture and vitamin K-dependent protein function in atherosclerosis.

The vitamin K oxidoreductase is a multimer that efficiently reduces vitamin K epoxide to hydroquinone to allow vitamin K-dependent protein carboxylation

The vitamin K-dependent carboxylase

The vitamin K-dependent carboxylase

The vitamin K-dependent carboxylase generates γ-carboxylated glutamates by using CO2 to facilitate glutamate deprotonation in a concerted mechanism that drives catalysis.

The vitamin K-dependent carboxylase has been acquired by Leptospira pathogens and shows altered activity that suggests a role other than protein carboxylation

VKOR paralog VKORC1L1 supports vitamin K-dependent protein carboxylation in vivo

Vitamin K oxygenation, glutamate carboxylation, and processivity: defining the three critical facets of catalysis by the vitamin K-dependent carboxylase

Vitamin K-dependent carboxylation

Vitamin K-dependent carboxylation of the carboxylase

Warfarin alters vitamin K metabolism: a surprising mechanism of VKORC1 uncoupling necessitates an additional reductase

r-VKORC1 expression in factor IX BHK cells increases the extent of factor IX carboxylation but is limited by saturation of another carboxylation component or by a shift in the rate-limiting step.