List of works - Alain Milon

15NT2′ relaxation times of bacteriorhodopsin transmembrane amide nitrogens

1H nuclear magnetic resonance determination of the membrane-bound conformation of senktide, a highly selective neurokinin B agonist ⬇️

scientific article published on July 1, 1993

A protein nanocontainer targeting epithelial cancers: rational engineering, biochemical characterization, drug loading and cell delivery

scientific article published on 01 February 2019

Ability of clionasterol and poriferasterol (24-epimers of sitosterol and stigmasterol) to regulate membrane lipid dynamics

Acyl chain order parameter profiles in phospholipid bilayers: computation from molecular dynamics simulations and comparison with 2H NMR experiments

scientific article published on 28 June 2007

Characterization of substance P-membrane interaction by transferred nuclear Overhauser effect

scientific article published in October 2000

Composition and phase behaviour of polar lipids isolated from Spirulina maxima cells grown in a perdeuterated medium

scientific article published on October 1996

Description of the low-affinity interaction between nociceptin and the second extracellular loop of its receptor by fluorescence and NMR spectroscopies.

scientific article published on November 2008

Detection of natural abundance 1H-13C correlations of cholesterol in its membrane environment using a gradient enhanced HSQC experiment under high resolution magic angle spinning

scientific article

Determination of the orientation and dynamics of ergosterol in model membranes using uniform 13C labeling and dynamically averaged 13C chemical shift anisotropies as experimental restraints.

scientific article

Deuterium-NMR investigation of plant sterol effects on soybean phosphatidylcholine acyl chain ordering

article

Differential binding to the alpha/beta-tubulin dimer of vinorelbine and vinflunine revealed by nuclear magnetic resonance analyses

scientific article published in August 2002

Dynamics of Klebsiella pneumoniae OmpA transmembrane domain: the four extracellular loops display restricted motion behavior in micelles and in lipid bilayers

scientific article

Engineering transglycosidase activity into a GH51 α-l-arabinofuranosidase

scientific article published on 27 April 2013

Expression and pharmacological characterization of the human mu-opioid receptor in the methylotrophic yeast Pichia pastoris

scientific article

Functional expression of the PorAH channel from Corynebacterium glutamicum in cell-free expression systems: implications for the role of the naturally occurring mycolic acid modification

scientific article

Functional roles of H98 and W99 and β2α2 loop dynamics in the α-l-arabinofuranosidase from Thermobacillus xylanilyticus.

scientific article

Fusogenic Alzheimer's peptide fragment Abeta (29-42) in interaction with lipid bilayers: secondary structure, dynamics, and specific interaction with phosphatidyl ethanolamine polar heads as revealed by solid-state NMR.

scientific article

GATEWAY™ technology and E. coli recombinant system produce a properly folded and functional recombinant allergen of the lipid transfer protein of apple (Mal d 3)

scientific article published on 22 September 2009

Generation of formate by the formyltransferase/hydrolase complex (Fhc) from Methylobacterium extorquens AM1.

scientific article

Giant vesicles as an efficient intermediate for 2H NMR analyses of proteoliposomes in water suspension and in oriented lipid bilayers

Green fluorescent protein as a reporter of human mu-opioid receptor overexpression and localization in the methylotrophic yeast Pichia pastoris

scientific article

Heterologous expression of G-protein-coupled receptors: comparison of expression systems from the standpoint of large-scale production and purification.

scientific article published on August 2003

Heterologous expression of a deuterated membrane-integrated receptor and partial deuteration in methylotrophic yeasts.

scientific article

High resolution 13C NMR spectra on oriented lipid bilayers: from quantifying the various sources of line broadening to performing 2D experiments with 0.2-0.3 ppm resolution in the carbon dimension

scientific article

High resolution 2D 1H-13C correlation of cholesterol in model membrane

scientific article published in September 2002

High-resolution 13C NMR of sterols in model membrane

Incorporation of phytosterols in human keratinocytes

scientific article published on 28 March 2006

Influence of Annexin V on the Structure and Dynamics of Phosphatidylcholine/Phosphatidylserine Bilayers: A Fluorescence and NMR Study†

article

Low cost production of perdeuterated biomass using methylotrophic yeasts

article

Methylobacterium extorquens AM1 produces a novel type of acyl-homoserine lactone with a double unsaturated side chain under methylotrophic growth conditions

scientific article published on 28 December 2005

Modelling the influence of hydrogen bond network on chemical shielding tensors description. GIAO-DFT study of WALP23 transmembrane alpha-helix as a test case

scientific article published on 12 May 2010

NMR studies of a new family of DNA binding proteins: the THAP proteins

scientific article

NMR-Based Structural Glycomics for High-Throughput Screening of Carbohydrate-Active Enzyme Specificity

scientific article published in 2011

Nuclear magnetic resonance analysis of protein-DNA interactions

scientific article published on 09 March 2011

Optimisation of plant sterols incorporation in human keratinocyte plasma membrane and modulation of membrane fluidity.

scientific article published on September 1999

Optimizing functional versus total expression of the human mu-opioid receptor in Pichia pastoris

scientific article

Order parameters of a transmembrane helix in a fluid bilayer: case study of a WALP peptide

scientific article

Partial atomic charges of amino acids in proteins

scientific article published in July 2004

Peptides as tools and drugs for immunotherapies

scientific article published on September 2007

Plant sterols: a neutron diffraction study of sitosterol and stigmasterol in soybean phosphatidylcholine membranes

article

Recombinant G protein-coupled receptors from expression to renaturation: a challenge towards structure

scientific article

Solubilization, purification, and mass spectrometry analysis of the human mu-opioid receptor expressed in Pichia pastoris

scientific article published in October 2005

Solution state NMR structure and dynamics of KpOmpA, a 210 residue transmembrane domain possessing a high potential for immunological applications

scientific article

Solution-State NMR Spectroscopy of Membrane Proteins in Detergent Micelles: Structure of the Klebsiella pneumoniae Outer Membrane Protein A, KpOmpA

scientific article published on 01 January 2010

Structural determinants of specific DNA-recognition by the THAP zinc finger

scientific article

Structural insights on the pamoic acid and the 8 kDa domain of DNA polymerase beta complex: towards the design of higher-affinity inhibitors

scientific article

Structural properties of a peptide derived from H+ -V-ATPase subunit a

scientific article

Structure and dynamics of G protein-coupled receptor-bound ghrelin reveal the critical role of the octanoyl chain

scientific article published on 15 August 2019

Structure-function analysis of the THAP zinc finger of THAP1, a large C2CH DNA-binding module linked to Rb/E2F pathways

scientific article

Structure/antigenicity relationship of cyclic and linear peptides mimicking the V3 loop of HTV2 envelope glycoprotein

scientific article published on 01 November 1998

Study of the specific lipid binding properties of Abeta 11-22 fragment at endosomal pH.

scientific article published in September 2009

Synthesis of Deuterium-Labeled Plant Sterols and Analysis of Their Side-Chain Mobility by Solid State Deuterium NMR

article

The N-terminal end truncated mu-opioid receptor: from expression to circular dichroism analysis

scientific article

The Ralstonia solanacearum pathogenicity regulator HrpB induces 3-hydroxy-oxindole synthesis

scholarly article

The conical shape of DIM lipids promotes Mycobacterium tuberculosis infection of macrophages

scientific article published on 22 November 2019

The full-length mu-opioid receptor: a conformational study by circular dichroism in trifluoroethanol and membrane-mimetic environments

scientific article

The transmembrane protein KpOmpA anchoring the outer membrane of Klebsiella pneumoniae unfolds and refolds in response to tensile load.

scientific article

Towards the classification of DYT6 dystonia mutants in the DNA-binding domain of THAP1.

scientific article published on 27 July 2012

Understanding sterol-membrane interactions part I: Hartree-Fock versus DFT calculations of 13C and 1H NMR isotropic chemical shifts of sterols in solution and analysis of hydrogen-bonding effects

scientific article published in November 2004

Understanding sterol-membrane interactions, part II: complete 1H and 13C assignments by solid-state NMR spectroscopy and determination of the hydrogen-bonding partners of cholesterol in a lipid bilayer

scientific article

Virtual and biophysical screening targeting the γ-tubulin complex--a new target for the inhibition of microtubule nucleation

scientific article

List of works by Alain Milon

15NT2′ relaxation times of bacteriorhodopsin transmembrane amide nitrogens

1H nuclear magnetic resonance determination of the membrane-bound conformation of senktide, a highly selective neurokinin B agonist ⬇️

A protein nanocontainer targeting epithelial cancers: rational engineering, biochemical characterization, drug loading and cell delivery

Ability of clionasterol and poriferasterol (24-epimers of sitosterol and stigmasterol) to regulate membrane lipid dynamics

Acyl chain order parameter profiles in phospholipid bilayers: computation from molecular dynamics simulations and comparison with 2H NMR experiments

Characterization of substance P-membrane interaction by transferred nuclear Overhauser effect

Composition and phase behaviour of polar lipids isolated from Spirulina maxima cells grown in a perdeuterated medium

Description of the low-affinity interaction between nociceptin and the second extracellular loop of its receptor by fluorescence and NMR spectroscopies.

Detection of natural abundance 1H-13C correlations of cholesterol in its membrane environment using a gradient enhanced HSQC experiment under high resolution magic angle spinning

Determination of the orientation and dynamics of ergosterol in model membranes using uniform 13C labeling and dynamically averaged 13C chemical shift anisotropies as experimental restraints.

Deuterium-NMR investigation of plant sterol effects on soybean phosphatidylcholine acyl chain ordering

Differential binding to the alpha/beta-tubulin dimer of vinorelbine and vinflunine revealed by nuclear magnetic resonance analyses

Dynamics of Klebsiella pneumoniae OmpA transmembrane domain: the four extracellular loops display restricted motion behavior in micelles and in lipid bilayers

Engineering transglycosidase activity into a GH51 α-l-arabinofuranosidase

Expression and pharmacological characterization of the human mu-opioid receptor in the methylotrophic yeast Pichia pastoris

Functional expression of the PorAH channel from Corynebacterium glutamicum in cell-free expression systems: implications for the role of the naturally occurring mycolic acid modification

Functional roles of H98 and W99 and β2α2 loop dynamics in the α-l-arabinofuranosidase from Thermobacillus xylanilyticus.

Fusogenic Alzheimer's peptide fragment Abeta (29-42) in interaction with lipid bilayers: secondary structure, dynamics, and specific interaction with phosphatidyl ethanolamine polar heads as revealed by solid-state NMR.

GATEWAY™ technology and E. coli recombinant system produce a properly folded and functional recombinant allergen of the lipid transfer protein of apple (Mal d 3)

Generation of formate by the formyltransferase/hydrolase complex (Fhc) from Methylobacterium extorquens AM1.

Giant vesicles as an efficient intermediate for 2H NMR analyses of proteoliposomes in water suspension and in oriented lipid bilayers

Green fluorescent protein as a reporter of human mu-opioid receptor overexpression and localization in the methylotrophic yeast Pichia pastoris

Heterologous expression of G-protein-coupled receptors: comparison of expression systems from the standpoint of large-scale production and purification.

Heterologous expression of a deuterated membrane-integrated receptor and partial deuteration in methylotrophic yeasts.

High resolution 13C NMR spectra on oriented lipid bilayers: from quantifying the various sources of line broadening to performing 2D experiments with 0.2-0.3 ppm resolution in the carbon dimension

High resolution 2D 1H-13C correlation of cholesterol in model membrane

High-resolution 13C NMR of sterols in model membrane

Incorporation of phytosterols in human keratinocytes

Influence of Annexin V on the Structure and Dynamics of Phosphatidylcholine/Phosphatidylserine Bilayers: A Fluorescence and NMR Study†

Low cost production of perdeuterated biomass using methylotrophic yeasts

Methylobacterium extorquens AM1 produces a novel type of acyl-homoserine lactone with a double unsaturated side chain under methylotrophic growth conditions

Modelling the influence of hydrogen bond network on chemical shielding tensors description. GIAO-DFT study of WALP23 transmembrane alpha-helix as a test case

NMR studies of a new family of DNA binding proteins: the THAP proteins

NMR-Based Structural Glycomics for High-Throughput Screening of Carbohydrate-Active Enzyme Specificity

Nuclear magnetic resonance analysis of protein-DNA interactions

Optimisation of plant sterols incorporation in human keratinocyte plasma membrane and modulation of membrane fluidity.

Optimizing functional versus total expression of the human mu-opioid receptor in Pichia pastoris

Order parameters of a transmembrane helix in a fluid bilayer: case study of a WALP peptide

Partial atomic charges of amino acids in proteins

Peptides as tools and drugs for immunotherapies

Plant sterols: a neutron diffraction study of sitosterol and stigmasterol in soybean phosphatidylcholine membranes

Recombinant G protein-coupled receptors from expression to renaturation: a challenge towards structure

Solubilization, purification, and mass spectrometry analysis of the human mu-opioid receptor expressed in Pichia pastoris

Solution state NMR structure and dynamics of KpOmpA, a 210 residue transmembrane domain possessing a high potential for immunological applications

Solution-State NMR Spectroscopy of Membrane Proteins in Detergent Micelles: Structure of the Klebsiella pneumoniae Outer Membrane Protein A, KpOmpA

Structural determinants of specific DNA-recognition by the THAP zinc finger

Structural insights on the pamoic acid and the 8 kDa domain of DNA polymerase beta complex: towards the design of higher-affinity inhibitors

Structural properties of a peptide derived from H+ -V-ATPase subunit a

Structure and dynamics of G protein-coupled receptor-bound ghrelin reveal the critical role of the octanoyl chain

Structure-function analysis of the THAP zinc finger of THAP1, a large C2CH DNA-binding module linked to Rb/E2F pathways

Structure/antigenicity relationship of cyclic and linear peptides mimicking the V3 loop of HTV2 envelope glycoprotein

Study of the specific lipid binding properties of Abeta 11-22 fragment at endosomal pH.

Synthesis of Deuterium-Labeled Plant Sterols and Analysis of Their Side-Chain Mobility by Solid State Deuterium NMR

The N-terminal end truncated mu-opioid receptor: from expression to circular dichroism analysis

The Ralstonia solanacearum pathogenicity regulator HrpB induces 3-hydroxy-oxindole synthesis

The conical shape of DIM lipids promotes Mycobacterium tuberculosis infection of macrophages

The full-length mu-opioid receptor: a conformational study by circular dichroism in trifluoroethanol and membrane-mimetic environments

The transmembrane protein KpOmpA anchoring the outer membrane of Klebsiella pneumoniae unfolds and refolds in response to tensile load.

Towards the classification of DYT6 dystonia mutants in the DNA-binding domain of THAP1.

Understanding sterol-membrane interactions part I: Hartree-Fock versus DFT calculations of 13C and 1H NMR isotropic chemical shifts of sterols in solution and analysis of hydrogen-bonding effects

Understanding sterol-membrane interactions, part II: complete 1H and 13C assignments by solid-state NMR spectroscopy and determination of the hydrogen-bonding partners of cholesterol in a lipid bilayer

Virtual and biophysical screening targeting the γ-tubulin complex--a new target for the inhibition of microtubule nucleation