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List of works by Stephen Douthwaite

A "bulged" double helix in a RNA-protein contact site.

scientific article

A novel Erm monomethyltransferase in antibiotic-resistant isolates of Mannheimia haemolytica and Pasteurella multocida

scientific article

A ribonuclease-resistant region of 5S RNA and its relation to the RNA binding sites of proteins L18 and L25 ⬇️

scientific article published on June 11, 1979

A single methyltransferase YefA (RlmCD) catalyses both m5U747 and m5U1939 modifications in Bacillus subtilis 23S rRNA

scientific journal article

Activity of the ketolide telithromycin is refractory to Erm monomethylation of bacterial rRNA.

scientific article published on June 2002

Allele-specific priming in the mapping of rRNA

scientific article published on 01 January 1998

Archease from Pyrococcus abyssi improves substrate specificity and solubility of a tRNA m5C methyltransferase

scientific article

Association of the novel aminoglycoside resistance determinant RmtF with NDM carbapenemase in Enterobacteriaceae isolated in India and the UK.

scientific article

Basis for prokaryotic specificity of action of aminoglycoside antibiotics

scientific article

Binding sites of ribosomal proteins on prokaryotic 5S RNAs: a study with ribonucleases

scientific article published on 01 May 1982

Biofilm Formation and Motility Are Promoted by Cj0588-Directed Methylation of rRNA in Campylobacter jejuni

scientific article published in January 2017

Bypassing rRNA methylation by RsmA/Dim1during ribosome maturation in the hyperthermophilic archaeon Nanoarchaeum equitans

scientific article published in February 2017

Capreomycin binds across the ribosomal subunit interface using tlyA-encoded 2'-O-methylations in 16S and 23S rRNAs

scientific article

Capreomycin susceptibility is increased by TlyA-directed 2'-O-methylation on both ribosomal subunits

scientific article

Characterization of the binding sites of protein L11 and the L10.(L12)4 pentameric complex in the GTPase domain of 23 S ribosomal RNA from Escherichia coli

scientific article published in May 1990

Comparison of Escherichia coli tRNAPhe in the Free State, in the Ternary Complex and in the Ribosomal A and P Sites by Chemical Probing

scientific article published on 01 March 1983

Complete Genome Sequence of Escherichia coli AS19, an Antibiotic-Sensitive Variant of E. coli Strain B REL606.

scientific article published on 3 May 2018

Cooperative assembly of proteins in the ribosomal GTPase centre demonstrated by their interactions with mutant 23S rRNAs.

scientific article published on July 1995

Core sequence in the RNA motif recognized by the ErmE methyltransferase revealed by relaxing the fidelity of the enzyme for its target.

scientific article published in January 1999

Crystal structure of the Escherichia coli 23S rRNA:m5C methyltransferase RlmI (YccW) reveals evolutionary links between RNA modification enzymes

scientific article

Defining the structural requirements for a helix in 23 S ribosomal RNA that confers erythromycin resistance.

scientific article

Design of cAMP-CRP-activated promoters in Escherichia coli.

scientific article published in February 1991

Designer drugs for discerning bugs ⬇️

scientific article published on September 27, 2010

Does unpaired adenosine-66 from helix II of Escherichia coli 5S RNA bind to protein L18?

scientific article published on April 1985

Domain V of 23S rRNA contains all the structural elements necessary for recognition by the ErmE methyltransferase

scientific article

Effect of mutations in the A site of 16 S rRNA on aminoglycoside antibiotic-ribosome interaction.

scientific article

ErmE methyltransferase recognition elements in RNA substrates 1 1Edited by D. E. Draper

scientific article published on 01 September 1998

ErmE methyltransferase recognizes features of the primary and secondary structure in a motif within domain V of 23 S rRNA.

scientific article

Erythromycin binding is reduced in ribosomes with conformational alterations in the 23 S rRNA peptidyl transferase loop

scientific article published in August 1993

Functional interactions within 23S rRNA involving the peptidyltransferase center.

scientific article published on February 1992

Helcococcus kunzii methyltransferase Erm(47) responsible for MLSB resistance is induced by diverse ribosome-targeting antibiotics

scientific article published on 01 February 2020

Higher order structure in the 3′-minor domain of small subunit ribosomal RNAs from a gram negative bacterium, a gram positive bacterium and a eukaryote

scientific article published on 01 September 1983

Higher-order structure in the 3′-terminal domain VI of the 23 S ribosomal RNAs from Escherichia coli and Bacillus stearothermophilus

scientific article published on 01 November 1984

Identification of the RsmG methyltransferase target as 16S rRNA nucleotide G527 and characterization of Bacillus subtilis rsmG mutants

scientific article

Identifying modifications in RNA by MALDI mass spectrometry

scientific article

Identifying the methyltransferases for m(5)U747 and m(5)U1939 in 23S rRNA using MALDI mass spectrometry

scientific article published in August 2003

Increased NADPH concentration obtained by metabolic engineering of the pentose phosphate pathway in Aspergillus niger

scientific article

Indigenous and acquired modifications in the aminoglycoside binding sites of Pseudomonas aeruginosa rRNAs

scientific article

Inhibition of protein synthesis on the ribosome by tildipirosin compared with other veterinary macrolides

scientific article

Integrative and Conjugative Elements (ICEs) in Pasteurellaceae Species and Their Detection by Multiplex PCR.

scientific article published on 26 June 2018

Interaction of the tylosin-resistance methyltransferase RlmA II at its rRNA target differs from the orthologue RlmA I.

scientific article

Interconversion of active and inactive 30 S ribosomal subunits is accompanied by a conformational change in the decoding region of 16 S rRNA.

scientific article

Isolation of temperature-sensitive mutants of 16 S rRNA in Escherichia coli.

scientific article published in October 1989

Ketolide antimicrobial activity persists after disruption of interactions with domain II of 23S rRNA.

scientific article published on October 2004

Ketolide resistance in Streptococcus pyogenes correlates with the degree of rRNA dimethylation by Erm.

scientific article

LNAzymes: incorporation of LNA-type monomers into DNAzymes markedly increases RNA cleavage.

scientific article published in November 2002

Locked nucleoside analogues expand the potential of DNAzymes to cleave structured RNA targets

scientific article

Macrolide resistance by ribosomal mutation in clinical isolates of Streptococcus pneumoniae from the PROTEKT 1999-2000 study

scientific article

Macrolide resistance conferred by base substitutions in 23S rRNA.

scientific article

Macrolide resistance conferred by rRNA mutations in field isolates of Mannheimia haemolytica and Pasteurella multocida

scientific article published on 25 September 2014

Macrolide-ketolide inhibition of MLS-resistant ribosomes is improved by alternative drug interaction with domain II of 23S rRNA

scientific article published on 01 April 2000

Methylation at nucleotide G745 or G748 in 23S rRNA distinguishes Gram-negative from Gram-positive bacteria

scientific article

Methylation of 23S rRNA nucleotide G745 is a secondary function of the RlmAI methyltransferase

scientific article

Methyltransferase Erm(37) slips on rRNA to confer atypical resistance in Mycobacterium tuberculosis

scientific article

Minimal substrate features for Erm methyltransferases defined by using a combinatorial oligonucleotide library.

scientific article

Molecular mechanisms by which rRNA mutations confer resistance to clindamycin

scientific article

Mutations in conserved helix 69 of 23S rRNA of Thermus thermophilus that affect capreomycin resistance but not posttranscriptional modifications

scientific article published on 19 September 2008

Mutations in domain II of 23 S rRNA facilitate translation of a 23 S rRNA-encoded pentapeptide conferring erythromycin resistance

scientific article published on May 1996

Mycobacterium smegmatis Erm(38) is a reluctant dimethyltransferase

scientific article

Negative in vitro selection identifies the rRNA recognition motif for ErmE methyltransferase

scientific article

Overexpression of the mitochondrial methyltransferase TFB1M in the mouse does not impact mitoribosomal methylation status or hearing

scientific article

Oxazolidinone resistance mutations in 23S rRNA of Escherichia coli reveal the central region of domain V as the primary site of drug action.

scientific article

Pseudoknot in domain II of 23 S rRNA is essential for ribosome function

scientific article published on May 1995

Recognition elements in rRNA for the tylosin resistance methyltransferase RlmA(II).

scientific article

Reductive Evolution and Diversification of C5-Uracil Methylation in the Nucleic Acids of Mollicutes

scientific article published on 10 April 2020

Resistance to ketolide antibiotics by coordinated expression of rRNA methyltransferases in a bacterial producer of natural ketolides

scientific article

Resistance to the macrolide antibiotic tylosin is conferred by single methylations at 23S rRNA nucleotides G748 and A2058 acting in synergy

scientific article

Ribosomal proteins L11 and L10.(L12)4 and the antibiotic thiostrepton interact with overlapping regions of the 23 S rRNA backbone in the ribosomal GTPase centre.

scientific article

Secondary structure of prokaryotic 5S ribosomal ribonucleic acids: a study with ribonucleases

scientific article published on 01 December 1981

Specific structural probing of plasmid-coded ribosomal RNAs from Escherichia coli

scientific article published on 01 December 1991

Specificity shifts in the rRNA and tRNA nucleotide targets of archaeal and bacterial m5U methyltransferases.

scientific article

Structure of 23S rRNA hairpin 35 and its interaction with the tylosin-resistance methyltransferase RlmAII.

scientific article

Structure of the bifunctional methyltransferase YcbY (RlmKL) that adds the m7G2069 and m2G2445 modifications in Escherichia coli 23S rRNA

scientific article

Structure-activity relationships of ketolides vs. macrolides

scientific article

Telithromycin resistance in Streptococcus pneumoniae is conferred by a deletion in the leader sequence of erm(B) that increases rRNA methylation

scientific article

The activity of rRNA resistance methyltransferases assessed by MALDI mass spectrometry

scientific article

The aminoglycoside resistance methyltransferase Sgm impedes RsmF methylation at an adjacent rRNA nucleotide in the ribosomal A site

scientific article

The antibiotics micrococcin and thiostrepton interact directly with 23S rRNA nucleotides 1067A and 1095A.

scientific article published on February 1994

The avilamycin resistance determinants AviRa and AviRb methylate 23S rRNA at the guanosine 2535 base and the uridine 2479 ribose

scientific article

The bacterial ribosome as a target for antibiotics

scientific article

The flavoprotein Mcap0476 (RlmFO) catalyzes m5U1939 modification in Mycoplasma capricolum 23S rRNA.

scientific article

The ketolides: a critical review

scientific article

The macrolide binding site on the bacterial ribosome

scientific article

The macrolide-ketolide antibiotic binding site is formed by structures in domains II and V of 23S ribosomal RNA.

scientific article

The ribosomal S4-RNA fragment melts cooperatively when complexed with protein S4 ⬇️

scientific article published on April 1, 1979

The structural basis of macrolide-ribosome binding assessed using mutagenesis of 23S rRNA positions 2058 and 2059.

scientific article

The structure of the RNA m5C methyltransferase YebU from Escherichia coli reveals a C-terminal RNA-recruiting PUA domain.

scientific article

The tylosin resistance gene tlrB of Streptomyces fradiae encodes a methyltransferase that targets G748 in 23S rRNA. ⬇️

scientific article

The tylosin-resistance methyltransferase RlmA(II) (TlrB) modifies the N-1 position of 23S rRNA nucleotide G748.

scientific article

Transcription analysis of the Streptomyces coelicolor A3(2) rrnA operon

scientific article published in December 1994

Visualizing the 16-membered ring macrolides tildipirosin and tilmicosin bound to their ribosomal site.

scientific article published on 14 May 2012

YbeA is the m3Psi methyltransferase RlmH that targets nucleotide 1915 in 23S rRNA.

scientific article published on 28 August 2008

YccW is the m5C methyltransferase specific for 23S rRNA nucleotide 1962.

scientific article

YebU is a m5C methyltransferase specific for 16 S rRNA nucleotide 1407.

scientific article

YgdE is the 2'-O-ribose methyltransferase RlmM specific for nucleotide C2498 in bacterial 23S rRNA.

scientific article

YibK is the 2'-O-methyltransferase TrmL that modifies the wobble nucleotide in Escherichia coli tRNA(Leu) isoacceptors

scientific article

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