List of works - Bruce Palfey

A fluoro analogue of the menadione derivative 6-[2'-(3'-methyl)-1',4'-naphthoquinolyl]hexanoic acid is a suicide substrate of glutathione reductase. Crystal structure of the alkylated human enzyme

scientific article

A single intersubunit salt bridge affects oligomerization and catalytic activity in a bacterial quinone reductase

scientific article

Adenosyltransferase tailors and delivers coenzyme B12.

scientific article published on 10 February 2008

Altered balance of half-reactions in p-hydroxybenzoate hydroxylase caused by substituting the 2'-carbon of FAD with fluorine

scientific article published on 8 April 2003

An analysis of the solution structure and signaling mechanism of LovK, a sensor histidine kinase integrating light and redox signals

scientific article

An unusual mechanism of thymidylate biosynthesis in organisms containing the thyX gene

scientific article

Analysis of the kinetic isotope effects on initial rates in transient kinetics

scientific article published in November 2006

Biochemical Establishment and Characterization of EncM's Flavin-N5-oxide Cofactor.

scientific article

Catalysis of diaphorase reactions by Mycobacterium tuberculosis lipoamide dehydrogenase occurs at the EH4 level

scientific journal article

Catalytic function of the conserved hydroxyl group in the protein tyrosine phosphatase signature motif.

scientific article published on December 1995

Comparing protein-ligand interactions in solution and single crystals by Raman spectroscopy

scientific article

Conformational dynamics of the isoalloxazine in substrate-free p-hydroxybenzoate hydroxylase: single-molecule studies

scientific article published on December 2005

Control of catalysis in flavin-dependent monooxygenases.

scientific article

Detection of intermediates in the oxidative half-reaction of the FAD-dependent thymidylate synthase from Thermotoga maritima: carbon transfer without covalent pyrimidine activation

scientific article published on 8 August 2014

Dihydrooxonate is a substrate of dihydroorotate dehydrogenase (DHOD) providing evidence for involvement of cysteine and serine residues in base catalysis

scientific article published in July 2001

Direct observation of the participation of flavin in product formation by thyX-encoded thymidylate synthase

scientific article published in January 2005

Disruption of the proton relay network in the class 2 dihydroorotate dehydrogenase from Escherichia coli

scientific article

Electrostatic effects on substrate activation in para-hydroxybenzoate hydroxylase: studies of the mutant lysine 297 methionine

scientific article published on 01 June 1997

Evidence for flavin movement in the function of p-hydroxybenzoate hydroxylase from studies of the mutant Arg220Lys.

scientific article

Flavin conformational changes in the catalytic cycle of p-hydroxybenzoate hydroxylase substituted with 6-azido- and 6-aminoflavin adenine dinucleotide

scientific article published on 01 December 1997

Flavin-mediated dual oxidation controls an enzymatic Favorskii-type rearrangement

scientific article

Graduate education chemical biology at the University of Michigan

scientific article published on 01 September 2006

Insight into the chemistry of flavin reduction and oxidation in Escherichia coli dihydroorotate dehydrogenase obtained by rapid reaction studies

scientific article published in April 2001

Interaction of benzoate pyrimidine analogues with class 1A dihydroorotate dehydrogenase from Lactococcus lactis

scientific article

Kinetic Analysis of Transient Intermediates in the Mechanism of Prenyl-Flavin-Dependent Ferulic Acid Decarboxylase

scientific article published on 19 December 2020

Kinetic mechanism and the rate-limiting step of Plasmodium vivax serine hydroxymethyltransferase

scientific article

Kinetic studies, mechanism, and substrate specificity of amadoriase I from Aspergillus sp

scientific article published on 01 October 2001

Kinetics of proton-linked flavin conformational changes in p-hydroxybenzoate hydroxylase

scientific article

Lot6p from Saccharomyces cerevisiae is a FMN-dependent reductase with a potential role in quinone detoxification

scientific article

Mechanism of dihydrouridine synthase 2 from yeast and the importance of modifications for efficient tRNA reduction

scientific article

Mechanism of flavin reduction in class 2 dihydroorotate dehydrogenases

scientific article

Mechanism of flavin reduction in the class 1A dihydroorotate dehydrogenase from Lactococcus lactis

scientific article published on 07 March 2007

Mechanistic insights into p-hydroxybenzoate hydroxylase from studies of the mutant Ser212Ala

scientific article published on 01 May 1999

Modelling flavin and substrate substituent effects on the activation barrier and rate of oxygen transfer by p-hydroxybenzoate hydroxylase

scientific article published on 01 July 2000

Multiple states of the Tyr318Leu mutant of dihydroorotate dehydrogenase revealed by single-molecule kinetics

scientific article

On the interpretation of quantitative structure-function activity relationship data for lactate oxidase

scientific article published in March 2000

On the reaction mechanism of L-lactate oxidase: quantitative structure-activity analysis of the reaction with para-substituted L-mandelates

scientific article published on September 1997

Oxidation mode of pyranose 2-oxidase is controlled by pH.

scientific article

Oxygen reactivity in flavoenzymes: context matters

scientific article

Probing the chemistries of the substrate and flavin ring system of p-hydroxybenzoate hydroxylase by raman difference spectroscopy

scientific article published on 01 October 1997

Protein dynamics control proton transfers to the substrate on the His72Asn mutant of p-hydroxybenzoate hydroxylase

scientific article published in April 2001

Quinone reductase acts as a redox switch of the 20S yeast proteasome

scientific article

Relationship between the time-dependence of a transient-state kinetic isotope effect and the location of complexes in a reaction sequence

scientific article published in April 2006

Role of protein flexibility in the catalytic cycle of p-hydroxybenzoate hydroxylase elucidated by the Pro293Ser mutant

scientific article published in July 2002

Roles in binding and chemistry for conserved active site residues in the class 2 dihydroorotate dehydrogenase from Escherichia coli

scientific article published on August 2009

Single-molecule kinetics reveals signatures of half-sites reactivity in dihydroorotate dehydrogenase A catalysis

scholarly article

Specific inhibition of a family 1A dihydroorotate dehydrogenase by benzoate pyrimidine analogues

scientific article published in August 2001

Substrate binding and reactivity are not linked: grafting a proton-transfer network into a Class 1A dihydroorotate dehydrogenase

scientific article

Substrate recognition by "password" in p-hydroxybenzoate hydroxylase

scientific article published on 01 January 1999

Tandem nitroaldol-dehydration reactions employing the dianion of phenylsulfonylnitromethane(1)

scientific article published on 01 November 2000

The cation-π interaction between Lys53 and the flavin of fructosamine oxidase (FAOX-II) is critical for activity

scientific article published on 25 August 2011

The dimeric dihydroorotate dehydrogenase A from Lactococcus lactis dissociates reversibly into inactive monomers

scientific article published on November 2002

The lipoamide dehydrogenase from Mycobacterium tuberculosis permits the direct observation of flavin intermediates in catalysis

scientific article published in December 2002

Trapping of an intermediate in the reaction catalyzed by flavin-dependent thymidylate synthase.

scientific article published on 24 February 2012

Tunable Heteroaromatic Sulfones Enhance in-Cell Cysteine Profiling

scientific article published on 13 January 2020

Two-Photon Excitation of Flavins and Flavoproteins with Classical and Quantum Light

article

Using Raman spectroscopy to monitor the solvent-exposed and "buried" forms of flavin in p-hydroxybenzoate hydroxylase

scientific article published on 01 December 1999

List of works by Bruce Palfey

A fluoro analogue of the menadione derivative 6-[2'-(3'-methyl)-1',4'-naphthoquinolyl]hexanoic acid is a suicide substrate of glutathione reductase. Crystal structure of the alkylated human enzyme

A single intersubunit salt bridge affects oligomerization and catalytic activity in a bacterial quinone reductase

Adenosyltransferase tailors and delivers coenzyme B12.

Altered balance of half-reactions in p-hydroxybenzoate hydroxylase caused by substituting the 2'-carbon of FAD with fluorine

An analysis of the solution structure and signaling mechanism of LovK, a sensor histidine kinase integrating light and redox signals

An unusual mechanism of thymidylate biosynthesis in organisms containing the thyX gene

Analysis of the kinetic isotope effects on initial rates in transient kinetics

Biochemical Establishment and Characterization of EncM's Flavin-N5-oxide Cofactor.

Catalysis of diaphorase reactions by Mycobacterium tuberculosis lipoamide dehydrogenase occurs at the EH4 level

Catalytic function of the conserved hydroxyl group in the protein tyrosine phosphatase signature motif.

Comparing protein-ligand interactions in solution and single crystals by Raman spectroscopy

Conformational dynamics of the isoalloxazine in substrate-free p-hydroxybenzoate hydroxylase: single-molecule studies

Control of catalysis in flavin-dependent monooxygenases.

Detection of intermediates in the oxidative half-reaction of the FAD-dependent thymidylate synthase from Thermotoga maritima: carbon transfer without covalent pyrimidine activation

Dihydrooxonate is a substrate of dihydroorotate dehydrogenase (DHOD) providing evidence for involvement of cysteine and serine residues in base catalysis

Direct observation of the participation of flavin in product formation by thyX-encoded thymidylate synthase

Disruption of the proton relay network in the class 2 dihydroorotate dehydrogenase from Escherichia coli

Electrostatic effects on substrate activation in para-hydroxybenzoate hydroxylase: studies of the mutant lysine 297 methionine

Evidence for flavin movement in the function of p-hydroxybenzoate hydroxylase from studies of the mutant Arg220Lys.

Flavin conformational changes in the catalytic cycle of p-hydroxybenzoate hydroxylase substituted with 6-azido- and 6-aminoflavin adenine dinucleotide

Flavin-mediated dual oxidation controls an enzymatic Favorskii-type rearrangement

Graduate education chemical biology at the University of Michigan

Insight into the chemistry of flavin reduction and oxidation in Escherichia coli dihydroorotate dehydrogenase obtained by rapid reaction studies

Interaction of benzoate pyrimidine analogues with class 1A dihydroorotate dehydrogenase from Lactococcus lactis

Kinetic Analysis of Transient Intermediates in the Mechanism of Prenyl-Flavin-Dependent Ferulic Acid Decarboxylase

Kinetic mechanism and the rate-limiting step of Plasmodium vivax serine hydroxymethyltransferase

Kinetic studies, mechanism, and substrate specificity of amadoriase I from Aspergillus sp

Kinetics of proton-linked flavin conformational changes in p-hydroxybenzoate hydroxylase

Lot6p from Saccharomyces cerevisiae is a FMN-dependent reductase with a potential role in quinone detoxification

Mechanism of dihydrouridine synthase 2 from yeast and the importance of modifications for efficient tRNA reduction

Mechanism of flavin reduction in class 2 dihydroorotate dehydrogenases

Mechanism of flavin reduction in the class 1A dihydroorotate dehydrogenase from Lactococcus lactis

Mechanistic insights into p-hydroxybenzoate hydroxylase from studies of the mutant Ser212Ala

Modelling flavin and substrate substituent effects on the activation barrier and rate of oxygen transfer by p-hydroxybenzoate hydroxylase

Multiple states of the Tyr318Leu mutant of dihydroorotate dehydrogenase revealed by single-molecule kinetics

On the interpretation of quantitative structure-function activity relationship data for lactate oxidase

On the reaction mechanism of L-lactate oxidase: quantitative structure-activity analysis of the reaction with para-substituted L-mandelates

Oxidation mode of pyranose 2-oxidase is controlled by pH.

Oxygen reactivity in flavoenzymes: context matters

Probing the chemistries of the substrate and flavin ring system of p-hydroxybenzoate hydroxylase by raman difference spectroscopy

Protein dynamics control proton transfers to the substrate on the His72Asn mutant of p-hydroxybenzoate hydroxylase

Quinone reductase acts as a redox switch of the 20S yeast proteasome

Relationship between the time-dependence of a transient-state kinetic isotope effect and the location of complexes in a reaction sequence

Role of protein flexibility in the catalytic cycle of p-hydroxybenzoate hydroxylase elucidated by the Pro293Ser mutant

Roles in binding and chemistry for conserved active site residues in the class 2 dihydroorotate dehydrogenase from Escherichia coli

Single-molecule kinetics reveals signatures of half-sites reactivity in dihydroorotate dehydrogenase A catalysis

Specific inhibition of a family 1A dihydroorotate dehydrogenase by benzoate pyrimidine analogues

Substrate binding and reactivity are not linked: grafting a proton-transfer network into a Class 1A dihydroorotate dehydrogenase

Substrate recognition by "password" in p-hydroxybenzoate hydroxylase

Tandem nitroaldol-dehydration reactions employing the dianion of phenylsulfonylnitromethane(1)

The cation-π interaction between Lys53 and the flavin of fructosamine oxidase (FAOX-II) is critical for activity

The dimeric dihydroorotate dehydrogenase A from Lactococcus lactis dissociates reversibly into inactive monomers

The lipoamide dehydrogenase from Mycobacterium tuberculosis permits the direct observation of flavin intermediates in catalysis

Trapping of an intermediate in the reaction catalyzed by flavin-dependent thymidylate synthase.

Tunable Heteroaromatic Sulfones Enhance in-Cell Cysteine Profiling

Two-Photon Excitation of Flavins and Flavoproteins with Classical and Quantum Light

Using Raman spectroscopy to monitor the solvent-exposed and "buried" forms of flavin in p-hydroxybenzoate hydroxylase