Search filters

List of works by Richard John Ward

A SequenceSpace analysis of Lys49 phopholipases A2: clues towards identification of residues involved in a novel mechanism of membrane damage and in myotoxicity

scientific article published in April 1998

A designed bifunctional laccase/β-1,3-1,4-glucanase enzyme shows synergistic sugar release from milled sugarcane bagasse

scientific article published on 25 September 2012

A micelle nucleation model for the interaction of dodecyl sulphate with Lys49-phospholipases A(2).

scientific article

A molecular mechanism for Lys49-phospholipase A2 activity based on ligand-induced conformational change.

scientific article published on 13 December 2004

A novel thermostable xylanase GH10 from Malbranchea pulchella expressed in Aspergillus nidulans with potential applications in biotechnology

scientific article

A pH-induced dissociation of the dimeric form of a lysine 49-phospholipase A2 abolishes Ca2+-independent membrane damaging activity

scientific article

A practical teaching course in directed protein evolution using the green fluorescent protein as a model.

scientific article

A predominant role for hydrogen bonding in the stability of the homodimer of bothropstoxin-I, A lysine 49-phospholipase A2.

scientific article published in November 2005

A stability transition at mildly acidic pH in the alpha-hemolysin (alpha-toxin) from Staphylococcus aureus

scientific article published on 01 October 1999

A xylose-stimulated xylanase-xylose binding protein chimera created by random nonhomologous recombination

scientific article

Activation of Ca2+-independent membrane-damaging activity in Lys49-phospholipase A2 promoted by amphiphilic molecules

scientific article

Active site mutants of human secreted Group IIA Phospholipase A2 lacking hydrolytic activity retain their bactericidal effect

scientific article published on 06 October 2011

Amino acid sequence of a myotoxic Lys49-phospholipase A2 homologue from the venom of Cerrophidion (Bothrops) godmani

scientific article published in May 1998

An ultraviolet photoacoustic spectroscopy study of the interaction between Lys49-phospholipase A2 and amphiphilic molecules.

scientific article

At the interface: crystal structures of phospholipases A2

scientific article published on 01 November 1998

Beauveria bassiana Lipase A expressed in Komagataella (Pichia) pastoris with potential for biodiesel catalysis.

scientific article

Biochemical properties of glycosylation and characterization of a histidine acid phosphatase (phytase) expressed in Pichia pastoris

scientific article published on 27 March 2014

Characterization and mRNA expression analysis of PI31, an endogenous proteasome inhibitor from Schistosoma mansoni.

scientific article published on 3 August 2010

Characterization of suramin binding sites on the human group IIA secreted phospholipase A2 by site-directed mutagenesis and molecular dynamics simulation

scientific article published on 10 January 2012

Characterization of temperature dependent and substrate-binding cleft movements in Bacillus circulans family 11 xylanase: a molecular dynamics investigation.

scientific article published on 4 May 2009

Chemical denaturation of a homodimeric lysine-49 phospholipase A2: a stable dimer interface and a native monomeric intermediate

scientific article published in March 2003

Concommitant adaptation of a GH11 xylanase by directed evolution to create an alkali-tolerant/thermophilic enzyme

scientific article

Conformation analysis of a surface loop that controls active site access in the GH11 xylanase A from Bacillus subtilis ⬇️

scientific article published on July 23, 2011

Correlation of temperature induced conformation change with optimum catalytic activity in the recombinant G/11 xylanase A from Bacillus subtilis strain 168 (1A1)

scientific article published on 02 November 2005

Crystal structure of piratoxin-I: a calcium-independent, myotoxic phospholipase A2-homologue from Bothrops pirajai venom

scientific article published on 01 October 1998

Crystallization and preliminary X-ray crystallographic studies of the mesophilic xylanase A from Bacillus subtilis 1A1.

scientific article published on 20 January 2005

Crystallization and preliminary diffraction data of bothropstoxin I isolated from the venom of Bothrops jararacussu ⬇️

scientific article published on March 1, 1995

Crystallization of bothropstoxin II isolated from the venom of Bothrops jararacussu

scientific article published on 01 May 1996

Crystallization of piratoxin I, a myotoxic Lys49-phospholipase A2 homologue isolated from the venom of Bothrops pirajai.

scientific article published in March 1998

D-xylose detection in Escherichia coli by a xylose binding protein-dependent response

scientific article published on 24 October 2013

Defective T-cell activation by Mycoplasma arthritidis mitogen is restored by interferon-gamma

scientific article published on 01 April 1989

Dissecting structure-function-stability relationships of a thermostable GH5-CBM3 cellulase from Bacillus subtilis 168

scientific article

Dissociation of enzymatic and pharmacological properties of piratoxins-I and -III, two myotoxic phospholipases A2 from Bothrops pirajai snake venom

scientific article published in March 2001

Effect of crotapotin on the biological activity of Asp49 and Lys49 phospholipases A(2) from Bothrops snake venoms

scientific article published in August 2004

Efficient constitutive expression of Bacillus subtilis xylanase A in Escherichia coli DH5alpha under the control of the Bacillus BsXA promoter

scientific article published in January 2006

Engineering bifunctional laccase-xylanase chimeras for improved catalytic performance.

scientific article

Engineering the pattern of protein glycosylation modulates the thermostability of a GH11 xylanase.

scientific article published on 11 July 2013

Enhanced xyloglucan-specific endo-β-1,4-glucanase efficiency in an engineered CBM44-XegA chimera

scientific article published on 22 January 2015

Expression and characterization of HlyX hemolysin from Leptospira interrogans serovar Copenhageni: potentiation of hemolytic activity by LipL32.

scientific article published in August 2005

Expression in E. coli and purification of the nucleoside diphosphate kinase b from Leishmania major.

scientific article

Functional characterization and oligomerization of a recombinant xyloglucan-specific endo-β-1,4-glucanase (GH12) from Aspergillus niveus

scientific article published in March 2012

GH53 Endo-Beta-1,4-Galactanase from a Newly Isolated Bacillus licheniformis CBMAI 1609 as an Enzymatic Cocktail Supplement for Biomass Saccharification

scientific article published on 15 February 2016

Identification of a putative membrane-inserted segment in the alpha-toxin of Staphylococcus aureus

scientific article published on 01 June 1994

Increased biomass saccharification by supplementation of a commercial enzyme cocktail with endo-arabinanase from Bacillus licheniformis.

scientific article

Insertion of a xylanase in xylose binding protein results in a xylose-stimulated xylanase

scientific article published on 15 August 2015

Insights on calcium-independent phospholipid membrane damage by Lys49-PLA2 using tryptophan scanning mutagenesis of bothropstoxin-I from Bothrops jararacussu

scientific article published on 4 November 2007

Isolation, purification, and physicochemical characterization of a D-galactose-binding lectin from seeds of Erythrina speciosa.

scientific article

Lignocellulose binding of a Cel5A-RtCBM11 chimera with enhanced β-glucanase activity monitored by electron paramagnetic resonance.

scientific article published on 14 November 2017

Lipid bilayer stabilization of the Na,K-ATPase reconstituted in DPPC/DPPE liposomes

scientific article published on 01 January 2006

Mapping of suramin binding sites on the group IIA human secreted phospholipase A2.

scientific article published on 3 February 2009

Mapping of the structural determinants of artificial and biological membrane damaging activities of a Lys49 phospholipase A2 by scanning alanine mutagenesis

scientific article published on 09 February 2007

Mapping structural determinants of biological activities in snake venom phospholipases A2 by sequence analysis and site directed mutagenesis.

scientific article published on December 2003

Method for forming two-dimensional paracrystals of biological filaments on lipid monolayers

scientific article

Molecular adaptability of nucleoside diphosphate kinase b from trypanosomatid parasites: stability, oligomerization and structural determinants of nucleotide binding

scientific article

Molecular modeling of the human eukaryotic translation initiation factor 5A (eIF5A) based on spectroscopic and computational analyses.

scientific article

Ontogenetic changes in Phoneutria nigriventer (Araneae, Ctenidae) spider venom

scientific article published on 01 November 2004

Permeabilization of E. coli K12 inner and outer membranes by bothropstoxin-I, A LYS49 phospholipase A2 from Bothrops jararacussu

scientific article published on 17 November 2007

Production, purification, crystallization and preliminary X-ray diffraction studies of the nucleoside diphosphate kinase b from Leishmania major

scientific article published on 30 October 2009

Properties of a purified thermostable glucoamylase from Aspergillus niveus.

scientific article published on 21 August 2009

Purification and biochemical characterization of a novel alpha-glucosidase from Aspergillus niveus

scientific article published in November 2009

Purification and biochemical characterization of a thermostable extracellular glucoamylase produced by the thermotolerant fungus Paecilomyces variotii

scientific article published on 16 October 2007

Purification and characterization of two xylanases from alkalophilic and thermophilic Bacillus licheniformis 77-2.

scientific article published in January 2006

Purification, some properties of a D-galactose-binding leaf lectin from Erythrina indica and further characterization of seed lectin.

scientific article

Reduced pH induces an inactive non-native conformation of the monomeric bothropstoxin-I (Lys49-PLA2).

scientific article published on 18 May 2009

Reflex changes in respiration and heart rate evoked by intravenous and left ventricular injection of 5-HT and capsaicin in anaesthetized rats: a comparison of mechanisms

scientific article published in January 1984

Refolding and purification of the human secreted group IID phospholipase A2 expressed as inclusion bodies in Escherichia coli

scientific article published on 17 April 2009

Sequence of a cDNA encoding bothropstoxin I, a myotoxin from the venom of Bothrops jararacussu

scientific article

Shared structural determinants for the calcium-independent liposome membrane permeabilization and sarcolemma depolarization in Bothropstoxin-I, a LYS49-PLA(2) from the venom of Bothrops jararacussu.

scientific article published in September 2009

Spectroscopic characterization and structural modeling of prolamin from maize and pearl millet

scientific article

Structural and functional analysis of BmjMIP, a phospholipase A2 myotoxin inhibitor protein from Bothrops moojeni snake plasma

scientific article

Structural basis for glucose tolerance in GH1 β-glucosidases

scientific article

Structural insights for fatty acid binding in a Lys49-phospholipase A2: crystal structure of myotoxin II from Bothrops moojeni complexed with stearic acid

scientific article published on 01 February 2005

Structure of a Lys49-Phospholipase A2 homologue isolated from the venom of Bothrops nummifer (jumping viper)

scientific article published on 01 February 1999

Structure of a calcium-independent phospholipase-like myotoxic protein from Bothrops asper venom

scientific article published in May 1995

Subproteomic analysis of soluble proteins of the microsomal fraction from two Leishmania species

scientific article published in September 2006

Synthetic biology approaches to improve biocatalyst identification in metagenomic library screening

scientific article

Tertiary structural changes of the alpha-hemolysin from Staphylococcus aureus on association with liposome membranes

scientific article published on 01 March 1998

The Staphylococcus aureus alpha-toxin channel complex and the effect of Ca2+ ions on its interaction with lipid layers

scientific article published on 01 September 1992

The amino acid sequence of ribitol dehydrogenase-F, a mutant enzyme with improved xylitol dehydrogenase activity

scientific article published on 01 May 1999

The association of Na,K-ATPase subunits studied by circular dichroism, surface tension and dilatational elasticity

scientific article published on 13 June 2008

The bactericidal effect of human secreted group IID phospholipase A2 results from both hydrolytic and non-hydrolytic activities.

scientific article

The biological activity in mammals and insects of the nucleosidic fraction from the spider Parawixia bistriata.

scientific article published in March 2004

The effect of resonance energy homotransfer on the intrinsic tryptophan fluorescence emission of the bothropstoxin-I dimer

scientific article published in June 2001

The interaction of bothropstoxin-I (Lys49-PLA(2)) with liposome membranes.

scientific article published on 3 June 2009

Thermostabilization of Bacillus subtilis GH11 xylanase by surface charge engineering

scientific article

Thermostable variants of the recombinant xylanase A from Bacillus subtilis produced by directed evolution show reduced heat capacity changes

scientific article

Paulina is supported by:

About Paulina

Help