List of works - Kensaku Sakamoto

A new protein engineering approach combining chemistry and biology, part I; site-specific incorporation of 4-iodo-L-phenylalanine in vitro by using misacylated suppressor tRNAPhe

scientific article published in October 2006

Adding l-lysine derivatives to the genetic code of mammalian cells with engineered pyrrolysyl-tRNA synthetases

scientific article published on 8 May 2008

An engineered Escherichia coli tyrosyl-tRNA synthetase for site-specific incorporation of an unnatural amino acid into proteins in eukaryotic translation and its application in a wheat germ cell-free system

scientific article

Cation-pi interaction in the polyolefin cyclization cascade uncovered by incorporating unnatural amino acids into the catalytic sites of squalene cyclase

scientific article

Cell-Free Protein Synthesis Using S30 Extracts from RFzero Strains for Efficient Incorporation of Non-Natural Amino Acids into Proteins

scientific article published in 2019

Codon reassignment in the Escherichia coli genetic code

scientific article

Crystal structure of bacterial RNA polymerase bound with a transcription inhibitor protein

scientific article

Crystal structure of eukaryotic translation initiation factor 2B

scientific article

Crystallographic studies on multiple conformational states of active-site loops in pyrrolysyl-tRNA synthetase

scientific article

Crystallographic study of a site-specifically cross-linked protein complex with a genetically incorporated photoreactive amino acid

scientific article

DNA polymerase programmed with a hairpin DNA incorporates a multiple-instruction architecture into molecular computing

scientific article published in January 2006

Dissecting cell signaling pathways with genetically encoded 3-iodo-L-tyrosine

scientific article published on 29 December 2010

Efficient decoding of the UAG triplet as a full-fledged sense codon enhances the growth of a prfA-deficient strain of Escherichia coli

scientific article

Engineering an Automaturing Transglutaminase with Enhanced Thermostability by Genetic Code Expansion with Two Codon Reassignments

scientific article published on 14 August 2018

Expanded genetic code technologies for incorporating modified lysine at multiple sites

scientific article published on September 2014

Fully Productive Cell-Free Genetic Code Expansion by Structure-Based Engineering of Methanomethylophilus alvus Pyrrolysyl-tRNA Synthetase

scientific article published on 17 March 2020

Functional replacement of the endogenous tyrosyl-tRNA synthetase-tRNATyr pair by the archaeal tyrosine pair in Escherichia coli for genetic code expansion

scientific article

Genetic encoding of 3-iodo-L-tyrosine in Escherichia coli for single-wavelength anomalous dispersion phasing in protein crystallography

scientific article

Genetic encoding of non-natural amino acids in Drosophila melanogaster Schneider 2 cells

scientific article

Genetic incorporation of a photo-crosslinkable amino acid reveals novel protein complexes with GRB2 in mammalian cells

scientific article published on 23 December 2010

Genetic-code evolution for protein synthesis with non-natural amino acids

scientific article

Highly reproductive Escherichia coli cells with no specific assignment to the UAG codon

scientific article

Incorporation of a Doubly Functionalized Synthetic Amino Acid into Proteins for Creating Chemical and Light-Induced Conjugates

scientific article published in December 2015

Indolmycin resistance of Streptomyces coelicolor A3(2) by induced expression of one of its two tryptophanyl-tRNA synthetases ⬇️

scientific article published on 22 April 2002

Intra- and inter-nucleosomal interactions of the histone H4 tail revealed with a human nucleosome core particle with genetically-incorporated H4 tetra-acetylation

scientific article

Multiple Site-Specific Installations ofNε-Monomethyl-L-Lysine into Histone Proteins by Cell-Based and Cell-Free Protein Synthesis

scientific article published in 2014

Multistep engineering of pyrrolysyl-tRNA synthetase to genetically encode N(epsilon)-(o-azidobenzyloxycarbonyl) lysine for site-specific protein modification

scientific article

Protein photo-cross-linking in mammalian cells by site-specific incorporation of a photoreactive amino acid

scientific article published on 17 February 2005

Protein stabilization utilizing a redefined codon

scientific article

Reassignment of a rare sense codon to a non-canonical amino acid in Escherichia coli

scientific article

Recognition of non-alpha-amino substrates by pyrrolysyl-tRNA synthetase

scientific article

Regioselective carbon-carbon bond formation in proteins with palladium catalysis; new protein chemistry by organometallic chemistry

scientific article published in January 2006

Site-Specific Functionalization of Proteins by Organopalladium Reactions

scholarly article by Koichiro Kodama published in January 2007

Site-specific incorporation of 4-iodo-L-phenylalanine through opal suppression

scientific article published on 21 May 2010

Site-specific incorporation of non-natural amino acids into proteins in mammalian cells with an expanded genetic code

Structural basis for extracellular interactions between calcitonin receptor-like receptor and receptor activity-modifying protein 2 for adrenomedullin-specific binding

scientific article

Structural basis for orthogonal tRNA specificities of tyrosyl-tRNA synthetases for genetic code expansion

scientific article (publication date: June 2003)

Structural basis of interleukin-5 dimer recognition by its α receptor

scientific article

Structural basis of nonnatural amino acid recognition by an engineered aminoacyl-tRNA synthetase for genetic code expansion

scientific article

Structural snapshots of the KMSKS loop rearrangement for amino acid activation by bacterial tyrosyl-tRNA synthetase

scientific article

Structure-based site-directed photo-crosslinking analyses of multimeric cell-adhesive interactions of voltage-gated sodium channel β subunits

scientific article published on 24 May 2016

Synthetic Tyrosine tRNA Molecules with Noncanonical Secondary Structures ⬇️

journal article from 'International Journal of Molecular Sciences' published in 2019

Tetrameric interaction of the ectoenzyme CD38 on the cell surface enables its catalytic and raft-association activities

scientific article

The Escherichia coli argU10(Ts) phenotype is caused by a reduction in the cellular level of the argU tRNA for the rare codons AGA and AGG.

scientific article published on September 2004

Transplantation of a tyrosine editing domain into a tyrosyl-tRNA synthetase variant enhances its specificity for a tyrosine analog

scholarly article

Wide-range protein photo-crosslinking achieved by a genetically encoded N(ε)-(benzyloxycarbonyl)lysine derivative with a diazirinyl moiety

scientific article published in February 2012

List of works by Kensaku Sakamoto

A new protein engineering approach combining chemistry and biology, part I; site-specific incorporation of 4-iodo-L-phenylalanine in vitro by using misacylated suppressor tRNAPhe

Adding l-lysine derivatives to the genetic code of mammalian cells with engineered pyrrolysyl-tRNA synthetases

An engineered Escherichia coli tyrosyl-tRNA synthetase for site-specific incorporation of an unnatural amino acid into proteins in eukaryotic translation and its application in a wheat germ cell-free system

Cation-pi interaction in the polyolefin cyclization cascade uncovered by incorporating unnatural amino acids into the catalytic sites of squalene cyclase

Cell-Free Protein Synthesis Using S30 Extracts from RFzero Strains for Efficient Incorporation of Non-Natural Amino Acids into Proteins

Codon reassignment in the Escherichia coli genetic code

Crystal structure of bacterial RNA polymerase bound with a transcription inhibitor protein

Crystal structure of eukaryotic translation initiation factor 2B

Crystallographic studies on multiple conformational states of active-site loops in pyrrolysyl-tRNA synthetase

Crystallographic study of a site-specifically cross-linked protein complex with a genetically incorporated photoreactive amino acid

DNA polymerase programmed with a hairpin DNA incorporates a multiple-instruction architecture into molecular computing

Dissecting cell signaling pathways with genetically encoded 3-iodo-L-tyrosine

Efficient decoding of the UAG triplet as a full-fledged sense codon enhances the growth of a prfA-deficient strain of Escherichia coli

Engineering an Automaturing Transglutaminase with Enhanced Thermostability by Genetic Code Expansion with Two Codon Reassignments

Expanded genetic code technologies for incorporating modified lysine at multiple sites

Fully Productive Cell-Free Genetic Code Expansion by Structure-Based Engineering of Methanomethylophilus alvus Pyrrolysyl-tRNA Synthetase

Functional replacement of the endogenous tyrosyl-tRNA synthetase-tRNATyr pair by the archaeal tyrosine pair in Escherichia coli for genetic code expansion

Genetic encoding of 3-iodo-L-tyrosine in Escherichia coli for single-wavelength anomalous dispersion phasing in protein crystallography

Genetic encoding of non-natural amino acids in Drosophila melanogaster Schneider 2 cells

Genetic incorporation of a photo-crosslinkable amino acid reveals novel protein complexes with GRB2 in mammalian cells

Genetic-code evolution for protein synthesis with non-natural amino acids

Highly reproductive Escherichia coli cells with no specific assignment to the UAG codon

Incorporation of a Doubly Functionalized Synthetic Amino Acid into Proteins for Creating Chemical and Light-Induced Conjugates

Indolmycin resistance of Streptomyces coelicolor A3(2) by induced expression of one of its two tryptophanyl-tRNA synthetases ⬇️

Intra- and inter-nucleosomal interactions of the histone H4 tail revealed with a human nucleosome core particle with genetically-incorporated H4 tetra-acetylation

Multiple Site-Specific Installations ofNε-Monomethyl-L-Lysine into Histone Proteins by Cell-Based and Cell-Free Protein Synthesis

Multistep engineering of pyrrolysyl-tRNA synthetase to genetically encode N(epsilon)-(o-azidobenzyloxycarbonyl) lysine for site-specific protein modification

Protein photo-cross-linking in mammalian cells by site-specific incorporation of a photoreactive amino acid

Protein stabilization utilizing a redefined codon

Reassignment of a rare sense codon to a non-canonical amino acid in Escherichia coli

Recognition of non-alpha-amino substrates by pyrrolysyl-tRNA synthetase

Regioselective carbon-carbon bond formation in proteins with palladium catalysis; new protein chemistry by organometallic chemistry

Site-Specific Functionalization of Proteins by Organopalladium Reactions

Site-specific incorporation of 4-iodo-L-phenylalanine through opal suppression

Site-specific incorporation of non-natural amino acids into proteins in mammalian cells with an expanded genetic code

Structural basis for extracellular interactions between calcitonin receptor-like receptor and receptor activity-modifying protein 2 for adrenomedullin-specific binding

Structural basis for orthogonal tRNA specificities of tyrosyl-tRNA synthetases for genetic code expansion

Structural basis of interleukin-5 dimer recognition by its α receptor

Structural basis of nonnatural amino acid recognition by an engineered aminoacyl-tRNA synthetase for genetic code expansion

Structural snapshots of the KMSKS loop rearrangement for amino acid activation by bacterial tyrosyl-tRNA synthetase

Structure-based site-directed photo-crosslinking analyses of multimeric cell-adhesive interactions of voltage-gated sodium channel β subunits

Synthetic Tyrosine tRNA Molecules with Noncanonical Secondary Structures ⬇️

Tetrameric interaction of the ectoenzyme CD38 on the cell surface enables its catalytic and raft-association activities

The Escherichia coli argU10(Ts) phenotype is caused by a reduction in the cellular level of the argU tRNA for the rare codons AGA and AGG.

Transplantation of a tyrosine editing domain into a tyrosyl-tRNA synthetase variant enhances its specificity for a tyrosine analog

Wide-range protein photo-crosslinking achieved by a genetically encoded N(ε)-(benzyloxycarbonyl)lysine derivative with a diazirinyl moiety