List of works - Pau Ferrer

A multi-level study of recombinant Pichia pastoris in different oxygen conditions.

scientific article

Amino acid biosynthesis and metabolic flux profiling of Pichia pastoris

scientific article

An improved secretion signal enhances the secretion of model proteins from Pichia pastoris

scientific article published on 12 October 2018

Biotechnological Production of the Cell Penetrating Antifungal PAF102 Peptide in Pichia pastoris

scientific article published on 27 June 2019

Carbohydrate Hydrolytic Potential and Redundancy of an Anaerobic Digestion Microbiome Exposed to Acidosis, as Uncovered by Metagenomics

scientific article published on 18 July 2019

Cloning, disruption and protein secretory phenotype of the GAS1 homologue of Pichia pastoris.

scientific article published in November 2006

Combined effect of the methanol utilization (Mut) phenotype and gene dosage on recombinant protein production in Pichia pastoris fed-batch cultures.

scientific article

Comprehensive clone screening and evaluation of fed-batch strategies in a microbioreactor and lab scale stirred tank bioreactor system: application on Pichia pastoris producing Rhizopus oryzae lipase

scientific article published on 7 March 2014

Contextualized genome-scale model unveils high-order metabolic effects of the specific growth rate and oxygenation level in recombinant Pichia pastoris

scientific article published on 14 October 2019

Deregulation of methanol metabolism reverts transcriptional limitations of recombinant Pichia pastoris (Komagataella spp) with multiple expression cassettes under control of the AOX1 promoter

scientific article published on 17 February 2019

Designing Chimeric Virus-like Particle-based Vaccines for Human Papillomavirus and HIV: Lessons Learned

scientific article published on 01 January 2019

Detection and Elimination of Cellular Bottlenecks in Protein-Producing Yeasts

scientific article published on 01 January 2019

Developing high cell density fed-batch cultivation strategies for heterologous protein production in Pichia pastoris using the nitrogen source-regulated FLD1 Promoter

scientific article published in September 2005

Development of an antibiotic-free plasmid selection system based on glycine auxotrophy for recombinant protein overproduction in Escherichia coli

scientific article

Development of quantitative metabolomics for Pichia pastoris.

scientific article published on 21 April 2011

Droplet digital PCR-aided screening and characterization of Pichia pastoris multiple gene copy strains

scientific article published on 24 December 2015

Effects of methanol on lipases: molecular, kinetic and process issues in the production of biodiesel.

scientific article

Engineering new mycobacterial vaccine design for HIV-TB pediatric vaccine vectored by lysine auxotroph of BCG

scientific article

Engineering of bottlenecks in Rhizopus oryzae lipase production in Pichia pastoris using the nitrogen source-regulated FLD1 promoter

scientific article published on 01 September 2009

Expression of a Rhizopus oryzae lipase in Pichia pastoris under control of the nitrogen source-regulated formaldehyde dehydrogenase promoter

scientific article published in April 2004

Fine-tuning the P. pastoris iMT1026 genome-scale metabolic model for improved prediction of growth on methanol or glycerol as sole carbon sources

scientific article published on 21 November 2017

Genome-scale metabolic model of methylotrophic yeast Pichia pastoris and its use for in silico analysis of heterologous protein production.

scientific article published in July 2010

Genome-scale metabolic reconstruction for the insidious bacterium in aquaculture Piscirickettsia salmonis.

scientific article

Glucose-methanol co-utilization in Pichia pastoris studied by metabolomics and instationary ¹³C flux analysis.

scientific article published on 28 February 2013

Glycerol metabolism of Pichia pastoris (Komagataella spp.) characterised by 13C-based metabolic flux analysis

scientific article published on 17 January 2019

Hypoxic fed-batch cultivation of Pichia pastoris increases specific and volumetric productivity of recombinant proteins

scientific article published in May 2008

Increased dosage of AOX1 promoter-regulated expression cassettes leads to transcription attenuation of the methanol metabolism in Pichia pastoris

scientific article published on 15 March 2017

Influence of growth temperature on the production of antibody Fab fragments in different microbes: a host comparative analysis.

scientific article published on 23 December 2010

Integration and Validation of the Genome-Scale Metabolic Models of Pichia pastoris: A Comprehensive Update of Protein Glycosylation Pathways, Lipid and Energy Metabolism

scientific article published on 26 January 2016

Integrative omics analysis of the termite gut system adaptation to Miscanthus diet identifies lignocellulose degradation enzymes

scientific article published on 01 June 2020

Macromolecular and elemental composition analysis and extracellular metabolite balances of Pichia pastoris growing at different oxygen levels.

scientific article published on 9 December 2009

Metabolic flux analysis and the NAD(P)H/NAD(P)+ ratios in chemostat cultures of Azotobacter vinelandii.

scientific article published on 22 January 2018

Metabolic flux analysis during the exponential growth phase of Saccharomyces cerevisiae in wine fermentations

scientific article

Metabolic flux profiling of Pichia pastoris grown on glycerol/methanol mixtures in chemostat cultures at low and high dilution rates

scientific article published in January 2007

Metabolic flux profiling of recombinant protein secreting Pichia pastoris growing on glucose:methanol mixtures.

scientific article published on 8 May 2012

Process optimization for recombinant protein production in the psychrophilic bacterium Pseudoalteromonas haloplanktis

article published in 2011

Production of fuculose-1-phosphate aldolase using operator-repressor titration for plasmid maintenance in high cell density Escherichia coli fermentations.

scientific article

Protein folding and conformational stress in microbial cells producing recombinant proteins: a host comparative overview

scientific article published on 4 April 2008

Protein trafficking, ergosterol biosynthesis and membrane physics impact recombinant protein secretion in Pichia pastoris ⬇️

scientific article published on November 3, 2011

Quantitative Metabolomics and Instationary 13C-Metabolic Flux Analysis Reveals Impact of Recombinant Protein Production on Trehalose and Energy Metabolism in Pichia pastoris

scientific article published on 5 May 2014

Quantitative metabolomics analysis of amino acid metabolism in recombinant Pichia pastoris under different oxygen availability conditions

scientific article published on 15 June 2012

Rational development of bioprocess engineering strategies for recombinant protein production in Pichia pastoris (Komagataella phaffii) using the methanol-free GAP promoter. Where do we stand?

scientific article published on 10 June 2019

Recombinant Candida rugosa lipase 2 from Pichia pastoris: immobilization and use as biocatalyst in a stereoselective reaction.

scientific article published on September 2010

Recombinant production of serine hydroxymethyl transferase from Streptococcus thermophilus and its preliminary evaluation as a biocatalyst

scientific article

Recombinant protein production 6: a comparative view on host physiology

scientific article published on 13 October 2011

Redox Engineering by Ectopic Overexpression of NADH Kinase in Recombinant Pichia pastoris (Komagataella phaffii): Impact on Cell Physiology and Recombinant Production of Secreted Proteins

scientific article published on 02 March 2020

Revisiting the Cellulosimicrobium cellulans yeast-lytic beta-1,3-glucanases toolbox: a review

scientific article

Rivoflavin may interfere with on-line monitoring of secreted green fluorescence protein fusion proteins in Pichia pastoris.

scientific article published on 18 May 2007

Sorbitol co-feeding reduces metabolic burden caused by the overexpression of a Rhizopus oryzae lipase in Pichia pastoris

scientific article published on 03 March 2007

Systems biology and biological systems diversity for the engineering of microbial cell factories.

scientific article published on 20 November 2007

The effect of hypoxia on the lipidome of recombinant Pichia pastoris.

scientific article

The effect of temperature on the proteome of recombinant Pichia pastoris

scientific article published in March 2009

The impact of oxygen on the transcriptome of recombinant S. cerevisiae and P. pastoris - a comparative analysis.

scientific article

Unconventional microbial systems for the cost-efficient production of high-quality protein therapeutics.

scientific article published on 15 September 2012

Use of chemostat cultures mimicking different phases of wine fermentations as a tool for quantitative physiological analysis.

scientific article published on 13 June 2014

Using promoter libraries to reduce metabolic burden due to plasmid-encoded proteins in recombinant Escherichia coli.

scientific article published on 31 August 2015

ZmXTH1, a new xyloglucan endotransglucosylase/hydrolase in maize, affects cell wall structure and composition in Arabidopsis thaliana

scientific article

¹³C-based metabolic flux analysis in yeast: the Pichia pastoris case

scientific article published in January 2014

¹³C-based metabolic flux analysis of recombinant Pichia pastoris.

scientific article published on January 2014

List of works by Pau Ferrer

A multi-level study of recombinant Pichia pastoris in different oxygen conditions.

Amino acid biosynthesis and metabolic flux profiling of Pichia pastoris

An improved secretion signal enhances the secretion of model proteins from Pichia pastoris

Biotechnological Production of the Cell Penetrating Antifungal PAF102 Peptide in Pichia pastoris

Carbohydrate Hydrolytic Potential and Redundancy of an Anaerobic Digestion Microbiome Exposed to Acidosis, as Uncovered by Metagenomics

Cloning, disruption and protein secretory phenotype of the GAS1 homologue of Pichia pastoris.

Combined effect of the methanol utilization (Mut) phenotype and gene dosage on recombinant protein production in Pichia pastoris fed-batch cultures.

Comprehensive clone screening and evaluation of fed-batch strategies in a microbioreactor and lab scale stirred tank bioreactor system: application on Pichia pastoris producing Rhizopus oryzae lipase

Contextualized genome-scale model unveils high-order metabolic effects of the specific growth rate and oxygenation level in recombinant Pichia pastoris

Deregulation of methanol metabolism reverts transcriptional limitations of recombinant Pichia pastoris (Komagataella spp) with multiple expression cassettes under control of the AOX1 promoter

Designing Chimeric Virus-like Particle-based Vaccines for Human Papillomavirus and HIV: Lessons Learned

Detection and Elimination of Cellular Bottlenecks in Protein-Producing Yeasts

Developing high cell density fed-batch cultivation strategies for heterologous protein production in Pichia pastoris using the nitrogen source-regulated FLD1 Promoter

Development of an antibiotic-free plasmid selection system based on glycine auxotrophy for recombinant protein overproduction in Escherichia coli

Development of quantitative metabolomics for Pichia pastoris.

Droplet digital PCR-aided screening and characterization of Pichia pastoris multiple gene copy strains

Effects of methanol on lipases: molecular, kinetic and process issues in the production of biodiesel.

Engineering new mycobacterial vaccine design for HIV-TB pediatric vaccine vectored by lysine auxotroph of BCG

Engineering of bottlenecks in Rhizopus oryzae lipase production in Pichia pastoris using the nitrogen source-regulated FLD1 promoter

Expression of a Rhizopus oryzae lipase in Pichia pastoris under control of the nitrogen source-regulated formaldehyde dehydrogenase promoter

Fine-tuning the P. pastoris iMT1026 genome-scale metabolic model for improved prediction of growth on methanol or glycerol as sole carbon sources

Genome-scale metabolic model of methylotrophic yeast Pichia pastoris and its use for in silico analysis of heterologous protein production.

Genome-scale metabolic reconstruction for the insidious bacterium in aquaculture Piscirickettsia salmonis.

Glucose-methanol co-utilization in Pichia pastoris studied by metabolomics and instationary ¹³C flux analysis.

Glycerol metabolism of Pichia pastoris (Komagataella spp.) characterised by 13C-based metabolic flux analysis

Hypoxic fed-batch cultivation of Pichia pastoris increases specific and volumetric productivity of recombinant proteins

Increased dosage of AOX1 promoter-regulated expression cassettes leads to transcription attenuation of the methanol metabolism in Pichia pastoris

Influence of growth temperature on the production of antibody Fab fragments in different microbes: a host comparative analysis.

Integration and Validation of the Genome-Scale Metabolic Models of Pichia pastoris: A Comprehensive Update of Protein Glycosylation Pathways, Lipid and Energy Metabolism

Integrative omics analysis of the termite gut system adaptation to Miscanthus diet identifies lignocellulose degradation enzymes

Macromolecular and elemental composition analysis and extracellular metabolite balances of Pichia pastoris growing at different oxygen levels.

Metabolic flux analysis and the NAD(P)H/NAD(P)+ ratios in chemostat cultures of Azotobacter vinelandii.

Metabolic flux analysis during the exponential growth phase of Saccharomyces cerevisiae in wine fermentations

Metabolic flux profiling of Pichia pastoris grown on glycerol/methanol mixtures in chemostat cultures at low and high dilution rates

Metabolic flux profiling of recombinant protein secreting Pichia pastoris growing on glucose:methanol mixtures.

Process optimization for recombinant protein production in the psychrophilic bacterium Pseudoalteromonas haloplanktis

Production of fuculose-1-phosphate aldolase using operator-repressor titration for plasmid maintenance in high cell density Escherichia coli fermentations.

Protein folding and conformational stress in microbial cells producing recombinant proteins: a host comparative overview

Protein trafficking, ergosterol biosynthesis and membrane physics impact recombinant protein secretion in Pichia pastoris ⬇️

Quantitative Metabolomics and Instationary 13C-Metabolic Flux Analysis Reveals Impact of Recombinant Protein Production on Trehalose and Energy Metabolism in Pichia pastoris

Quantitative metabolomics analysis of amino acid metabolism in recombinant Pichia pastoris under different oxygen availability conditions

Rational development of bioprocess engineering strategies for recombinant protein production in Pichia pastoris (Komagataella phaffii) using the methanol-free GAP promoter. Where do we stand?

Recombinant Candida rugosa lipase 2 from Pichia pastoris: immobilization and use as biocatalyst in a stereoselective reaction.

Recombinant production of serine hydroxymethyl transferase from Streptococcus thermophilus and its preliminary evaluation as a biocatalyst

Recombinant protein production 6: a comparative view on host physiology

Redox Engineering by Ectopic Overexpression of NADH Kinase in Recombinant Pichia pastoris (Komagataella phaffii): Impact on Cell Physiology and Recombinant Production of Secreted Proteins

Revisiting the Cellulosimicrobium cellulans yeast-lytic beta-1,3-glucanases toolbox: a review

Rivoflavin may interfere with on-line monitoring of secreted green fluorescence protein fusion proteins in Pichia pastoris.

Sorbitol co-feeding reduces metabolic burden caused by the overexpression of a Rhizopus oryzae lipase in Pichia pastoris

Systems biology and biological systems diversity for the engineering of microbial cell factories.

The effect of hypoxia on the lipidome of recombinant Pichia pastoris.

The effect of temperature on the proteome of recombinant Pichia pastoris

The impact of oxygen on the transcriptome of recombinant S. cerevisiae and P. pastoris - a comparative analysis.

Unconventional microbial systems for the cost-efficient production of high-quality protein therapeutics.

Use of chemostat cultures mimicking different phases of wine fermentations as a tool for quantitative physiological analysis.

Using promoter libraries to reduce metabolic burden due to plasmid-encoded proteins in recombinant Escherichia coli.

ZmXTH1, a new xyloglucan endotransglucosylase/hydrolase in maize, affects cell wall structure and composition in Arabidopsis thaliana

¹³C-based metabolic flux analysis in yeast: the Pichia pastoris case

¹³C-based metabolic flux analysis of recombinant Pichia pastoris.