List of works - Alexander Shekhtman

A full-length group 1 bacterial sigma factor adopts a compact structure incompatible with DNA binding

scientific article published on October 2008

A new member of the endonuclease III family of DNA repair enzymes that removes methylated purines from DNA

scientific article published on 01 June 1999

A novel, specific interaction involving the Csk SH3 domain and its natural ligand

scientific article

A ubiquitin-interacting motif from Hrs binds to and occludes the ubiquitin surface necessary for polyubiquitination in monoubiquitinated proteins

scientific article

ARTS binds to a distinct domain in XIAP-BIR3 and promotes apoptosis by a mechanism that is different from other IAP-antagonists

scientific article

Active metabolism unmasks functional protein-protein interactions in real time in-cell NMR

scientific article published on 21 May 2020

Advanced Glycation End Product Recognition by the Receptor for AGEs

scientific article

Analogs of the antituberculous agent pyrazinamide are competitive inhibitors of NADPH binding to M. tuberculosis fatty acid synthase I.

scientific article

Autoregulation of a bacterial sigma factor explored by using segmental isotopic labeling and NMR

scientific article

Backbone assignments of mini-RecA intein with short native exteins and an active N-terminal catalytic cysteine.

scientific article

Backbone dynamics of cyclotide MCoTI-I free and complexed with trypsin.

scientific article

Biosynthesis and antimicrobial evaluation of backbone-cyclized α-defensins

scientific article published on 9 November 2011

Biosynthesis and biological screening of a genetically encoded library based on the cyclotide MCoTI-I.

scientific article published in November 2009

Biosynthesis of a fully functional cyclotide inside living bacterial cells.

scientific article published in August 2007

Both Ca2+ and Zn2+ are essential for S100A12 protein oligomerization and function

scientific article

Caught in action: selecting peptide aptamers against intrinsically disordered proteins in live cells.

scientific article published on 24 March 2015

Cellular mechanisms and consequences of glycation in atherosclerosis and obesity

scientific article published on 7 May 2016

Change in the Molecular Dimension of a RAGE-Ligand Complex Triggers RAGE Signaling.

scientific article published on 9 August 2016

Chemical activation of adenylyl cyclase Rv1625c inhibits growth of Mycobacterium tuberculosis on cholesterol and modulates intramacrophage signaling.

scientific article published on 02 May 2017

Cobalt-based paramagnetic probe to study RNA-protein interactions by NMR.

scientific article published on 24 February 2017

Combinatorial library of improved peptide aptamers, CLIPs to inhibit RAGE signal transduction in mammalian cells.

scientific article

Cu(II)-Based Paramagnetic Probe to Study RNA-Protein Interactions by NMR.

scientific article

Design of a MCoTI-Based Cyclotide with Angiotensin (1-7)-Like Activity.

scientific article published on 26 January 2016

Disulfide bridges remain intact while native insulin converts into amyloid fibrils

scientific article

Efficient one-pot cyclization/folding of Rhesus θ-defensin-1 (RTD-1)

scientific article

Efficient recombinant expression of SFTI-1 in bacterial cells using intein-mediated protein trans-splicing

scientific article

Evaluation of potential genotoxicity of HIV entry inhibitors derived from natural sources

scientific article

Expression of fluorescent cyclotides using protein trans-splicing for easy monitoring of cyclotide-protein interactions

scientific article published on 15 January 2013

Fate of pup inside the Mycobacterium proteasome studied by in-cell NMR.

scientific article

Full Sequence Amino Acid Scanning of θ-Defensin RTD-1 Yields a Potent Anthrax Lethal Factor Protease Inhibitor

scientific article

Glycation & the RAGE axis: targeting signal transduction through DIAPH1.

scientific article published on 14 December 2016

Helical Structure of Recombinant Melittin

scientific article published on 04 January 2019

Hexameric calgranulin C (S100A12) binds to the receptor for advanced glycated end products (RAGE) using symmetric hydrophobic target-binding patches

scientific article published on 11 December 2006

In Vivo Activation of the p53 Tumor Suppressor Pathway by an Engineered Cyclotide

scientific article

In-Cell Protein NMR Spectroscopy

In-cell NMR for protein-protein interactions (STINT-NMR).

scientific article published in January 2006

In-cell NMR spectroscopy

scientific article published on 17 November 2010

In-cell biochemistry using NMR spectroscopy

scientific article

Increased Hydrophobicity and Decreased Backbone Flexibility Explain the Lower Solubility of a Cataract-Linked Mutant of γD-Crystallin ⬇️

scientific article published on July 30, 2011

Inhibition of HIV-1 infection in ex vivo cervical tissue model of human vagina by palmitic acid; implications for a microbicide development

scientific article

Intact ribosomes drive the formation of protein quinary structure

scientific article published on 24 April 2020

Mapping structural interactions using in-cell NMR spectroscopy (STINT-NMR).

scientific article published on February 2006

Molecular mechanism of the chaperone function of mini-α-crystallin, a 19-residue peptide of human α-crystallin.

scientific article

NMR determination that an extended BH3 motif of pro-apoptotic BID is specifically bound to BCL-XL.

scientific article published in July 2006

NMR structure determination and investigation using a reduced proton (REDPRO) labeling strategy for proteins

scientific article published on 01 July 2002

Novel mechanism of regulation of the non-receptor protein tyrosine kinase Csk: insights from NMR mapping studies and site-directed mutagenesis

scientific article

Nuclear Magnetic Resonance-Based Structural Characterization and Backbone Dynamics of Recombinant Bee Venom Melittin.

scientific article published on 18 April 2018

Palmitic acid analogs exhibit nanomolar binding affinity for the HIV-1 CD4 receptor and nanomolar inhibition of gp120-to-CD4 fusion

scientific article

Peptide aptamers: development and applications

scientific article

Potent Inhibitors of Mycobacterium tuberculosis Growth Identified by Using in-Cell NMR-based Screening

scientific article

Probing protein quinary interactions by in-cell nuclear magnetic resonance spectroscopy

scientific article

Protein chemical ligation as an invaluable tool for structural NMR.

scientific article

Proton (neutron) spin rotation in a polarized nuclear target: Method for investigating nuclear interactions

scientific article published on 01 January 1996

Pyrazinamide, but not pyrazinoic acid, is a competitive inhibitor of NADPH binding to Mycobacterium tuberculosis fatty acid synthase I

scientific article

Real-Time In-Cell Nuclear Magnetic Resonance: Ribosome-Targeted Antibiotics Modulate Quinary Protein Interactions

scientific article published on 21 December 2017

Recombinant Expression and Phenotypic Screening of a Bioactive Cyclotide Against α-Synuclein-Induced Cytotoxicity in Baker's Yeast.

scientific article published on 10 June 2015

Recombinant production of rhesus θ-defensin-1 (RTD-1) using a bacterial expression system.

scientific article

Ribosome Mediated Quinary Interactions Modulate In-Cell Protein Activities

scientific article

Screening of small molecule interactor library by using in-cell NMR spectroscopy (SMILI-NMR).

scientific article

Segmental isotopic labeling for structural biological applications of NMR.

scientific article

Segmental labeling to study multidomain proteins.

scientific article

Semisynthesis of a segmental isotopically labeled protein splicing precursor: NMR evidence for an unusual peptide bond at the N-extein-intein junction

scientific article

Signal transduction in receptor for advanced glycation end products (RAGE)

scientific article published in 2012

Small Molecule Inhibition of Ligand-Stimulated RAGE-DIAPH1 Signal Transduction

scientific article

Spartin activates atrophin-1-interacting protein 4 (AIP4) E3 ubiquitin ligase and promotes ubiquitination of adipophilin on lipid droplets

scientific article

Specific RNA-protein interactions detected with saturation transfer difference NMR ⬇️

scientific article published on July 30, 2013

Structural and Mechanical Properties of Amyloid Beta Fibrils: A Combined Experimental and Theoretical Approach

scientific article published on 7 July 2016

Structural basis for pattern recognition by the receptor for advanced glycation end products (RAGE).

scientific article

Structural biology: Inside the living cell

scientific article published on March 2009

Structural determination of biomolecular interfaces by nuclear magnetic resonance of proteins with reduced proton density

scientific article published on 7 April 2010

Structure of proteins in eukaryotic compartments

scientific article

The Receptor for Advanced Glycation End Products (RAGE) Specifically Recognizes Methylglyoxal-Derived AGEs

scientific article

The STINT-NMR method for studying in-cell protein-protein interactions.

scientific article

The cataract-associated V41M mutant of human γS-crystallin shows specific structural changes that directly enhance local surface hydrophobicity.

scientific article published on 25 November 2013

The multiple faces of RAGE--opportunities for therapeutic intervention in aging and chronic disease

scientific article

The role of spartin and its novel ubiquitin binding region in DALIS occurrence.

scientific article published on 12 February 2014

Total Cellular RNA Modulates Protein Activity.

scientific article published on 26 July 2016

Using singular value decomposition to characterize protein-protein interactions by in-cell NMR spectroscopy.

scientific article published on April 2014

X-linked Inhibitor of Apoptosis Protein promotes the degradation of its antagonist, the pro-apoptotic ARTS protein

scientific article

List of works by Alexander Shekhtman

A full-length group 1 bacterial sigma factor adopts a compact structure incompatible with DNA binding

A new member of the endonuclease III family of DNA repair enzymes that removes methylated purines from DNA

A novel, specific interaction involving the Csk SH3 domain and its natural ligand

A ubiquitin-interacting motif from Hrs binds to and occludes the ubiquitin surface necessary for polyubiquitination in monoubiquitinated proteins

ARTS binds to a distinct domain in XIAP-BIR3 and promotes apoptosis by a mechanism that is different from other IAP-antagonists

Active metabolism unmasks functional protein-protein interactions in real time in-cell NMR

Advanced Glycation End Product Recognition by the Receptor for AGEs

Analogs of the antituberculous agent pyrazinamide are competitive inhibitors of NADPH binding to M. tuberculosis fatty acid synthase I.

Autoregulation of a bacterial sigma factor explored by using segmental isotopic labeling and NMR

Backbone assignments of mini-RecA intein with short native exteins and an active N-terminal catalytic cysteine.

Backbone dynamics of cyclotide MCoTI-I free and complexed with trypsin.

Biosynthesis and antimicrobial evaluation of backbone-cyclized α-defensins

Biosynthesis and biological screening of a genetically encoded library based on the cyclotide MCoTI-I.

Biosynthesis of a fully functional cyclotide inside living bacterial cells.

Both Ca2+ and Zn2+ are essential for S100A12 protein oligomerization and function

Caught in action: selecting peptide aptamers against intrinsically disordered proteins in live cells.

Cellular mechanisms and consequences of glycation in atherosclerosis and obesity

Change in the Molecular Dimension of a RAGE-Ligand Complex Triggers RAGE Signaling.

Chemical activation of adenylyl cyclase Rv1625c inhibits growth of Mycobacterium tuberculosis on cholesterol and modulates intramacrophage signaling.

Cobalt-based paramagnetic probe to study RNA-protein interactions by NMR.

Combinatorial library of improved peptide aptamers, CLIPs to inhibit RAGE signal transduction in mammalian cells.

Cu(II)-Based Paramagnetic Probe to Study RNA-Protein Interactions by NMR.

Design of a MCoTI-Based Cyclotide with Angiotensin (1-7)-Like Activity.

Disulfide bridges remain intact while native insulin converts into amyloid fibrils

Efficient one-pot cyclization/folding of Rhesus θ-defensin-1 (RTD-1)

Efficient recombinant expression of SFTI-1 in bacterial cells using intein-mediated protein trans-splicing

Evaluation of potential genotoxicity of HIV entry inhibitors derived from natural sources

Expression of fluorescent cyclotides using protein trans-splicing for easy monitoring of cyclotide-protein interactions

Fate of pup inside the Mycobacterium proteasome studied by in-cell NMR.

Full Sequence Amino Acid Scanning of θ-Defensin RTD-1 Yields a Potent Anthrax Lethal Factor Protease Inhibitor

Glycation & the RAGE axis: targeting signal transduction through DIAPH1.

Helical Structure of Recombinant Melittin

Hexameric calgranulin C (S100A12) binds to the receptor for advanced glycated end products (RAGE) using symmetric hydrophobic target-binding patches

In Vivo Activation of the p53 Tumor Suppressor Pathway by an Engineered Cyclotide

In-Cell Protein NMR Spectroscopy

In-cell NMR for protein-protein interactions (STINT-NMR).

In-cell NMR spectroscopy

In-cell biochemistry using NMR spectroscopy

Increased Hydrophobicity and Decreased Backbone Flexibility Explain the Lower Solubility of a Cataract-Linked Mutant of γD-Crystallin ⬇️

Inhibition of HIV-1 infection in ex vivo cervical tissue model of human vagina by palmitic acid; implications for a microbicide development

Intact ribosomes drive the formation of protein quinary structure

Mapping structural interactions using in-cell NMR spectroscopy (STINT-NMR).

Molecular mechanism of the chaperone function of mini-α-crystallin, a 19-residue peptide of human α-crystallin.

NMR determination that an extended BH3 motif of pro-apoptotic BID is specifically bound to BCL-XL.

NMR structure determination and investigation using a reduced proton (REDPRO) labeling strategy for proteins

Novel mechanism of regulation of the non-receptor protein tyrosine kinase Csk: insights from NMR mapping studies and site-directed mutagenesis

Nuclear Magnetic Resonance-Based Structural Characterization and Backbone Dynamics of Recombinant Bee Venom Melittin.

Palmitic acid analogs exhibit nanomolar binding affinity for the HIV-1 CD4 receptor and nanomolar inhibition of gp120-to-CD4 fusion

Peptide aptamers: development and applications

Potent Inhibitors of Mycobacterium tuberculosis Growth Identified by Using in-Cell NMR-based Screening

Probing protein quinary interactions by in-cell nuclear magnetic resonance spectroscopy

Protein chemical ligation as an invaluable tool for structural NMR.

Proton (neutron) spin rotation in a polarized nuclear target: Method for investigating nuclear interactions

Pyrazinamide, but not pyrazinoic acid, is a competitive inhibitor of NADPH binding to Mycobacterium tuberculosis fatty acid synthase I

Real-Time In-Cell Nuclear Magnetic Resonance: Ribosome-Targeted Antibiotics Modulate Quinary Protein Interactions

Recombinant Expression and Phenotypic Screening of a Bioactive Cyclotide Against α-Synuclein-Induced Cytotoxicity in Baker's Yeast.

Recombinant production of rhesus θ-defensin-1 (RTD-1) using a bacterial expression system.

Ribosome Mediated Quinary Interactions Modulate In-Cell Protein Activities

Screening of small molecule interactor library by using in-cell NMR spectroscopy (SMILI-NMR).

Segmental isotopic labeling for structural biological applications of NMR.

Segmental labeling to study multidomain proteins.

Semisynthesis of a segmental isotopically labeled protein splicing precursor: NMR evidence for an unusual peptide bond at the N-extein-intein junction

Signal transduction in receptor for advanced glycation end products (RAGE)

Small Molecule Inhibition of Ligand-Stimulated RAGE-DIAPH1 Signal Transduction

Spartin activates atrophin-1-interacting protein 4 (AIP4) E3 ubiquitin ligase and promotes ubiquitination of adipophilin on lipid droplets

Specific RNA-protein interactions detected with saturation transfer difference NMR ⬇️

Structural and Mechanical Properties of Amyloid Beta Fibrils: A Combined Experimental and Theoretical Approach

Structural basis for pattern recognition by the receptor for advanced glycation end products (RAGE).

Structural biology: Inside the living cell

Structural determination of biomolecular interfaces by nuclear magnetic resonance of proteins with reduced proton density

Structure of proteins in eukaryotic compartments

The Receptor for Advanced Glycation End Products (RAGE) Specifically Recognizes Methylglyoxal-Derived AGEs

The STINT-NMR method for studying in-cell protein-protein interactions.

The cataract-associated V41M mutant of human γS-crystallin shows specific structural changes that directly enhance local surface hydrophobicity.

The multiple faces of RAGE--opportunities for therapeutic intervention in aging and chronic disease

The role of spartin and its novel ubiquitin binding region in DALIS occurrence.

Total Cellular RNA Modulates Protein Activity.

Using singular value decomposition to characterize protein-protein interactions by in-cell NMR spectroscopy.

X-linked Inhibitor of Apoptosis Protein promotes the degradation of its antagonist, the pro-apoptotic ARTS protein