List of works - Karen N. Allen

A Central Region of NF-κB Essential Modulator Is Required for IKKβ-Induced Conformational Change and for Signal Propagation

scientific article published on 18 June 2019

A Structural Element That Facilitates Proton-Coupled Electron Transfer in Oxalate Decarboxylase

scientific article

A cross-over inhibitor of the botulinum neurotoxin light chain B: a natural product implicating an exosite mechanism of action ⬇️

scientific article published on January 4, 2011

Assigning the EPR Fine Structure Parameters of the Mn(II) Centers in Bacillus subtilis Oxalate Decarboxylase by Site-Directed Mutagenesis and DFT/MM Calculations

scientific article

Binding Energy and Catalysis by d -Xylose Isomerase: Kinetic, Product, and X-ray Crystallographic Analysis of Enzyme-Catalyzed Isomerization of ( R )-Glyceraldehyde

scientific article

Catalytic cycling in beta-phosphoglucomutase: a kinetic and structural analysis

scientific article published in July 2005

Catalytic scaffolds for phosphoryl group transfer

scientific article published on 12 August 2016

Caught in the act: the structure of phosphorylated beta-phosphoglucomutase from Lactococcus lactis

scientific article

Chemical confirmation of a pentavalent phosphorane in complex with beta-phosphoglucomutase

scientific article published in April 2005

Chemical-modification rescue assessed by mass spectrometry demonstrates that gamma-thia-lysine yields the same activity as lysine in aldolase

scientific article published on July 2002

Co-evolution of HAD phosphatase and hotdog-fold thioesterase domain function in the menaquinone-pathway fusion proteins BF1314 and PG1653.

scientific article published on 10 July 2013

Conformational Cycling in β-Phosphoglucomutase Catalysis: Reorientation of the β-d-Glucose 1,6-(Bis)phosphate Intermediate†

scientific article published on 01 June 2006

Consequences of domain insertion on sequence-structure divergence in a superfold

scientific article published on 19 August 2013

Conservation and Covariance in Small Bacterial Phosphoglycosyltransferases Identify the Functional Catalytic Core

scientific article published on 24 November 2015

Crystallization and preliminary X-ray diffraction studies of beta-phosphoglucomutase from Lactococcus lactus

scientific article published on 24 January 2002

Crystallization and preliminary crystallographic analysis of phosphonoacetaldehyde hydrolase

Crystallography Coupled with Kinetic Analysis Provides Mechanistic Underpinnings of a Nicotine-Degrading Enzyme

scientific article published on 13 June 2018

Design of a heterospecific, tetrameric, 21-residue miniprotein with mixed alpha/beta structure.

scientific article

Disulfide-mediated stabilization of the IκB kinase binding domain of NF-κB essential modulator (NEMO).

scientific article

Diversification of function in the haloacid dehalogenase enzyme superfamily: The role of the cap domain in hydrolytic phosphoruscarbon bond cleavage

scientific article published on 27 October 2006

Double-lanthanide-binding tags for macromolecular crystallographic structure determination

scientific article

Encoded loop-lanthanide-binding tags for long-range distance measurements in proteins by NMR and EPR spectroscopy

scientific article published on 04 September 2015

Engineering encodable lanthanide-binding tags into loop regions of proteins

scientific article

Enzymatic synthesis of radiolabeled phosphonoacetaldehyde

scientific article published in November 2003

Enzyme Substrate Specificity Conferred by Distinct Conformational Pathways

scientific article published on 21 October 2015

Enzyme promiscuity: engine of evolutionary innovation.

scientific article

Evaluation of adamantane hydroxamates as botulinum neurotoxin inhibitors: Synthesis, crystallography, modeling, kinetic and cellular based studies ⬇️

scientific article published on December 20, 2012

Evolutionary genomics of the HAD superfamily: understanding the structural adaptations and catalytic diversity in a superfamily of phosphoesterases and allied enzymes

scientific article

Form finds function

scientific article published on 01 August 2007

HAD superfamily phosphotransferase substrate diversification: structure and function analysis of HAD subclass IIB sugar phosphatase BT4131.

scientific article

Intein-mediated purification of a recombinantly expressed peptide

scientific article published on 23 September 2004

Interaction Energetics and Druggability of the Protein-Protein Interaction between Kelch-like ECH-Associated Protein 1 (KEAP1) and Nuclear Factor Erythroid 2 Like 2 (Nrf2)

scientific article published on 02 January 2020

Investigation of metal ion binding in phosphonoacetaldehyde hydrolase identifies sequence markers for metal-activated enzymes of the HAD enzyme superfamily

scientific article published in May 2004

Lanthanide-Binding Tags for 3D X-ray Imaging of Proteins in Cells at Nanoscale Resolution

scientific article published on 21 January 2020

Mechanism of Substrate Recognition and Catalysis of the Haloalkanoic Acid Dehalogenase Family Member α-Phosphoglucomutase

scientific article published on 13 July 2018

Membrane association of monotopic phosphoglycosyl transferase underpins function

scientific article published on 16 May 2018

Metal Ions Effectively Ablate the Action of Botulinum Neurotoxin A.

scientific article published on 5 May 2017

Monotopic Membrane Proteins Join the Fold

scientific article published on 15 October 2018

Mutation of nonessential cysteines shows that the NF-κB essential modulator forms a constitutive noncovalent dimer that binds IκB kinase-β with high affinity.

scientific article published on 10 December 2013

Neutron diffraction studies towards deciphering the protonation state of catalytic residues in the bacterial KDN9P phosphatase.

scientific article published on 19 August 2013

New superfamily members identified for Schiff-base enzymes based on verification of catalytically essential residues.

scientific article

Panoramic view of a superfamily of phosphatases through substrate profiling

scientific article published on 6 April 2015

Quaternary ammonium oxidative demethylation: X-ray crystallographic, resonance Raman, and UV-visible spectroscopic analysis of a Rieske-type demethylase

scientific article

Spatial clustering of isozyme-specific residues reveals unlikely determinants of isozyme specificity in fructose-1,6-bisphosphate aldolase

scientific article (publication date: 9 May 2003)

Structural Basis for the Divergence of Substrate Specificity and Biological Function within HAD Phosphatases in Lipopolysaccharide and Sialic Acid Biosynthesis

scientific article

Structural Basis of the Molecular Switch between Phosphatase and Mutase Functions of Human Phosphomannomutase 1 under Ischemic Conditions

scientific article published on 11 May 2018

Structural Origin of the High Affinity of a Chemically Evolved Lanthanide-Binding Peptide

scientific article published on 01 July 2004

Structural and mechanistic themes in glycoconjugate biosynthesis at membrane interfaces

scientific article published on 16 April 2019

Structure and activity analyses of Escherichia coli K-12 NagD provide insight into the evolution of biochemical function in the haloalkanoic acid dehalogenase superfamily

scientific article published in January 2006

Structure of a fructose-1,6-bis(phosphate) aldolase liganded to its natural substrate in a cleavage-defective mutant at 2.3 A(,)

scientific article

Structure of a rabbit muscle fructose-1,6-bisphosphate aldolase A dimer variant

scientific article

Structure of the trehalose-6-phosphate phosphatase from Brugia malayi reveals key design principles for anthelmintic drugs

scientific article

Structure-Function Analysis of 2-Keto-3-deoxy-D-glycero-D-galactonononate-9-phosphate Phosphatase Defines Specificity Elements in Type C0 Haloalkanoate Dehalogenase Family Members

scientific article

Structure-Guided Insight into the Specificity and Mechanism of a Parasitic Nucleoside Hydrolase

scientific article published in 2022

Structure-guided approach for detecting large domain inserts in protein sequences as illustrated using the haloacid dehalogenase superfamily.

scientific article published on 2 May 2014

Substrate Binding Mode and Molecular Basis of a Specificity Switch in Oxalate Decarboxylase.

scientific article

The 2.1 A structure of Torpedo californica creatine kinase complexed with the ADP-Mg(2+)-NO(3)(-)-creatine transition-state analogue complex

scientific article

The Enzyme Function Initiative

scientific article

The X-ray crystal structures of human alpha-phosphomannomutase 1 reveal the structural basis of congenital disorder of glycosylation type 1a

scientific article

The X-ray crystallographic structure and activity analysis of a Pseudomonas-specific subfamily of the HAD enzyme superfamily evidences a novel biochemical function

scientific article

The X‐ray crystallographic structure and specificity profile of HAD superfamily phosphohydrolase BT1666: Comparison of paralogous functions in B. thetaiotaomicron ⬇️

scientific article published on August 30, 2011

The origin of the electrostatic perturbation in acetoacetate decarboxylase

scientific article

X-ray crystal structure of the hypothetical phosphotyrosine phosphatase MDP-1 of the haloacid dehalogenase superfamily

scientific article published in October 2004

X-ray structure analysis of a designed oligomeric miniprotein reveals a discrete quaternary architecture.

scholarly article

List of works by Karen N. Allen

A Central Region of NF-κB Essential Modulator Is Required for IKKβ-Induced Conformational Change and for Signal Propagation

A Structural Element That Facilitates Proton-Coupled Electron Transfer in Oxalate Decarboxylase

A cross-over inhibitor of the botulinum neurotoxin light chain B: a natural product implicating an exosite mechanism of action ⬇️

Assigning the EPR Fine Structure Parameters of the Mn(II) Centers in Bacillus subtilis Oxalate Decarboxylase by Site-Directed Mutagenesis and DFT/MM Calculations

Binding Energy and Catalysis by d -Xylose Isomerase: Kinetic, Product, and X-ray Crystallographic Analysis of Enzyme-Catalyzed Isomerization of ( R )-Glyceraldehyde

Catalytic cycling in beta-phosphoglucomutase: a kinetic and structural analysis

Catalytic scaffolds for phosphoryl group transfer

Caught in the act: the structure of phosphorylated beta-phosphoglucomutase from Lactococcus lactis

Chemical confirmation of a pentavalent phosphorane in complex with beta-phosphoglucomutase

Chemical-modification rescue assessed by mass spectrometry demonstrates that gamma-thia-lysine yields the same activity as lysine in aldolase

Co-evolution of HAD phosphatase and hotdog-fold thioesterase domain function in the menaquinone-pathway fusion proteins BF1314 and PG1653.

Conformational Cycling in β-Phosphoglucomutase Catalysis: Reorientation of the β-d-Glucose 1,6-(Bis)phosphate Intermediate†

Consequences of domain insertion on sequence-structure divergence in a superfold

Conservation and Covariance in Small Bacterial Phosphoglycosyltransferases Identify the Functional Catalytic Core

Crystallization and preliminary X-ray diffraction studies of beta-phosphoglucomutase from Lactococcus lactus

Crystallization and preliminary crystallographic analysis of phosphonoacetaldehyde hydrolase

Crystallography Coupled with Kinetic Analysis Provides Mechanistic Underpinnings of a Nicotine-Degrading Enzyme

Design of a heterospecific, tetrameric, 21-residue miniprotein with mixed alpha/beta structure.

Disulfide-mediated stabilization of the IκB kinase binding domain of NF-κB essential modulator (NEMO).

Diversification of function in the haloacid dehalogenase enzyme superfamily: The role of the cap domain in hydrolytic phosphoruscarbon bond cleavage

Double-lanthanide-binding tags for macromolecular crystallographic structure determination

Encoded loop-lanthanide-binding tags for long-range distance measurements in proteins by NMR and EPR spectroscopy

Engineering encodable lanthanide-binding tags into loop regions of proteins

Enzymatic synthesis of radiolabeled phosphonoacetaldehyde

Enzyme Substrate Specificity Conferred by Distinct Conformational Pathways

Enzyme promiscuity: engine of evolutionary innovation.

Evaluation of adamantane hydroxamates as botulinum neurotoxin inhibitors: Synthesis, crystallography, modeling, kinetic and cellular based studies ⬇️

Evolutionary genomics of the HAD superfamily: understanding the structural adaptations and catalytic diversity in a superfamily of phosphoesterases and allied enzymes

Form finds function

HAD superfamily phosphotransferase substrate diversification: structure and function analysis of HAD subclass IIB sugar phosphatase BT4131.

Intein-mediated purification of a recombinantly expressed peptide

Interaction Energetics and Druggability of the Protein-Protein Interaction between Kelch-like ECH-Associated Protein 1 (KEAP1) and Nuclear Factor Erythroid 2 Like 2 (Nrf2)

Investigation of metal ion binding in phosphonoacetaldehyde hydrolase identifies sequence markers for metal-activated enzymes of the HAD enzyme superfamily

Lanthanide-Binding Tags for 3D X-ray Imaging of Proteins in Cells at Nanoscale Resolution

Mechanism of Substrate Recognition and Catalysis of the Haloalkanoic Acid Dehalogenase Family Member α-Phosphoglucomutase

Membrane association of monotopic phosphoglycosyl transferase underpins function

Metal Ions Effectively Ablate the Action of Botulinum Neurotoxin A.

Monotopic Membrane Proteins Join the Fold

Mutation of nonessential cysteines shows that the NF-κB essential modulator forms a constitutive noncovalent dimer that binds IκB kinase-β with high affinity.

Neutron diffraction studies towards deciphering the protonation state of catalytic residues in the bacterial KDN9P phosphatase.

New superfamily members identified for Schiff-base enzymes based on verification of catalytically essential residues.

Panoramic view of a superfamily of phosphatases through substrate profiling

Quaternary ammonium oxidative demethylation: X-ray crystallographic, resonance Raman, and UV-visible spectroscopic analysis of a Rieske-type demethylase

Spatial clustering of isozyme-specific residues reveals unlikely determinants of isozyme specificity in fructose-1,6-bisphosphate aldolase

Structural Basis for the Divergence of Substrate Specificity and Biological Function within HAD Phosphatases in Lipopolysaccharide and Sialic Acid Biosynthesis

Structural Basis of the Molecular Switch between Phosphatase and Mutase Functions of Human Phosphomannomutase 1 under Ischemic Conditions

Structural Origin of the High Affinity of a Chemically Evolved Lanthanide-Binding Peptide

Structural and mechanistic themes in glycoconjugate biosynthesis at membrane interfaces

Structure and activity analyses of Escherichia coli K-12 NagD provide insight into the evolution of biochemical function in the haloalkanoic acid dehalogenase superfamily

Structure of a fructose-1,6-bis(phosphate) aldolase liganded to its natural substrate in a cleavage-defective mutant at 2.3 A(,)

Structure of a rabbit muscle fructose-1,6-bisphosphate aldolase A dimer variant

Structure of the trehalose-6-phosphate phosphatase from Brugia malayi reveals key design principles for anthelmintic drugs

Structure-Function Analysis of 2-Keto-3-deoxy-D-glycero-D-galactonononate-9-phosphate Phosphatase Defines Specificity Elements in Type C0 Haloalkanoate Dehalogenase Family Members

Structure-Guided Insight into the Specificity and Mechanism of a Parasitic Nucleoside Hydrolase

Structure-guided approach for detecting large domain inserts in protein sequences as illustrated using the haloacid dehalogenase superfamily.

Substrate Binding Mode and Molecular Basis of a Specificity Switch in Oxalate Decarboxylase.

The 2.1 A structure of Torpedo californica creatine kinase complexed with the ADP-Mg(2+)-NO(3)(-)-creatine transition-state analogue complex

The Enzyme Function Initiative

The X-ray crystal structures of human alpha-phosphomannomutase 1 reveal the structural basis of congenital disorder of glycosylation type 1a

The X-ray crystallographic structure and activity analysis of a Pseudomonas-specific subfamily of the HAD enzyme superfamily evidences a novel biochemical function

The X‐ray crystallographic structure and specificity profile of HAD superfamily phosphohydrolase BT1666: Comparison of paralogous functions in B. thetaiotaomicron ⬇️

The origin of the electrostatic perturbation in acetoacetate decarboxylase

X-ray crystal structure of the hypothetical phosphotyrosine phosphatase MDP-1 of the haloacid dehalogenase superfamily

X-ray structure analysis of a designed oligomeric miniprotein reveals a discrete quaternary architecture.