List of works - Emily Parker

A Pseudoisostructural Type II DAH7PS Enzyme from Pseudomonas aeruginosa: Alternative Evolutionary Strategies to Control Shikimate Pathway Flux

scientific article published on 2 April 2018

A dimeric catalytic core relates the short and long forms of ATP-phosphoribosyltransferase.

scientific article published on 5 December 2017

A single amino acid substitution uncouples catalysis and allostery in an essential biosynthetic enzyme in Mycobacterium tuberculosis

scientific article published on 26 March 2020

A symbiosis expressed non-ribosomal peptide synthetase from a mutualistic fungal endophyte of perennial ryegrass confers protection to the symbiotum from insect herbivory

scientific article

Active site plasticity of a critical enzyme from Mycobacterium tuberculosis

scholarly article by Sebastian Reichau & Emily J. Parker published 2013 in RSC Advances

Allosteric ACTion: the varied ACT domains regulating enzymes of amino-acid metabolism.

scientific article

Allosteric inhibitor specificity of Thermotoga maritima 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase

scientific article published on 31 July 2013

Alternative substrates reveal catalytic cycle and key binding events in the reaction catalysed by anthranilate phosphoribosyltransferase from Mycobacterium tuberculosis

scientific article

Amino Acid Biosynthesis

Amino-acid substitutions at the domain interface affect substrate and allosteric inhibitor binding in α-isopropylmalate synthase from Mycobacterium tuberculosis

scientific article published on 13 March 2013

Aminoacylation of Indole Diterpenes by Cluster-Specific Monomodular NRPS-like Enzymes

scientific article published on 22 March 2022

An extended β7α7 substrate-binding loop is essential for efficient catalysis by 3-deoxy-D-manno-octulosonate 8-phosphate synthase

scientific article

Anthranilate phosphoribosyltransferase: Binding determinants for 5'-phospho-alpha-d-ribosyl-1'-pyrophosphate (PRPP) and the implications for inhibitor design.

scientific article published on 24 August 2017

Arabinose 5-phosphate analogues as mechanistic probes for Neisseria meningitidis 3-deoxy-D-manno-octulosonate 8-phosphate synthase

scientific article

Arg314 is essential for catalysis by N-acetyl neuraminic acid synthase from Neisseria meningitidis

scientific article

Biochemical and structural characterisation of dehydroquinate synthase from the New Zealand kiwifruit Actinidia chinensis

scientific article

Biosynthesis of Nodulisporic Acids: A Multifunctional Monooxygenase Delivers a Complex and Highly Branched Array

scientific article published on 9 November 2022

Calculated pKa Variations Expose Dynamic Allosteric Communication Networks

scientific article

Campylobacter jejuni adenosine triphosphate phosphoribosyltransferase is an active hexamer that is allosterically controlled by the twisting of a regulatory tail

scientific article published on 18 May 2016

Change in Heat Capacity for Enzyme Catalysis Determines Temperature Dependence of Enzyme Catalyzed Rates

scientific article published on 21 February 2017

Change in heat capacity for enzyme catalysis determines temperature dependence of enzyme catalyzed rates

scientific article

Characterization of a recombinant type II 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase from Helicobacter pylori

scientific article published on August 2005

Cloning and characterisation of dihydrodipicolinate synthase from the pathogen Neisseria meningitidis

scientific article

Complex Formation between Two Biosynthetic Enzymes Modifies the Allosteric Regulatory Properties of Both: AN EXAMPLE OF MOLECULAR SYMBIOSIS.

scientific article published on June 2015

Conformational interdomain flexibility in a bacterial α-isopropylmalate synthase is necessary for leucine biosynthesis

scientific article published in February 2023

Crystal structure of type II NADH:quinone oxidoreductase from Caldalkalibacillus thermarum with an improved resolution of 2.15 Å.

scientific article published on 23 September 2017

Crystallization and preliminary X-ray crystallographic analysis of 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase from Mycobacterium tuberculosis

scientific article published on 24 March 2005

Datasets, processing and refinement details for Mtb-AnPRT: inhibitor structures with various space groups

scientific article

Defining paxilline biosynthesis in Penicillium paxilli: functional characterization of two cytochrome P450 monooxygenases

scientific article

Deletion and gene expression analyses define the paxilline biosynthetic gene cluster in Penicillium paxilli

scientific article

Derailing Dehydroquinate Synthase by Introducing a Stabilizing Stereoelectronic Effect in a Reaction Intermediate ⬇️

scientific article published on November 28, 1997

Destabilization of the homotetrameric assembly of 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase from the hyperthermophile Pyrococcus furiosus enhances enzymatic activity

scientific article

Diverse allosteric componentry and mechanisms control entry into aromatic metabolite biosynthesis

scientific article published on 30 July 2020

Domain cross-talk within a bifunctional enzyme provides catalytic and allosteric functionality in the biosynthesis of aromatic amino acids

scientific article published on 22 January 2019

Draft Genome Sequence of the Filamentous Fungus Hypoxylon pulicicidum ATCC 74245.

scientific article

Dynamic cross-talk among remote binding sites: the molecular basis for unusual synergistic allostery

scientific article

Editorial overview: Catalysis and regulation

scientific article published on 08 November 2020

Editorial overview: Catalysis and regulation: enzyme catalysis, biosynthetic pathways and regulation

scientific article published on 2 December 2014

Engineering allosteric control to an unregulated enzyme by transfer of a regulatory domain

scientific article

Enzymatic synthesis of (6R)- and (6S)-fluoroshikimic acids

scientific article published in 1995

Enzymic synthesis of 3-[3- 13C]dehydroquinic acid

scientific article published in October 2003

Erratum to “Reversing Evolution: Re-establishing Obligate Metal Ion Dependence in a Metal-independent KDO8P Synthase” [J. Mol. Biol. 390 (2009) 646–661]

scholarly article published in Journal of Molecular Biology

Examining the role of intersubunit contacts in catalysis by 3-deoxy-d-manno-octulosonate 8-phosphate synthase

scientific article

Exploring modular allostery via interchangeable regulatory domains

scientific article published on 5 March 2018

Expression, Purification, and Characterisation of Dehydroquinate Synthase from Pyrococcus furiosus

scientific article

Expression, purification, and characterization of 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase from Pyrococcus furiosus

scientific article published in March 2004

First structure of full-length mammalian phenylalanine hydroxylase reveals the architecture of an autoinhibited tetramer

scientific article published on 16 February 2016

Fluorinated substrates result in variable leakage of a reaction intermediate during catalysis by dehydroquinate synthase

scientific article

Four gene products are required for the fungal synthesis of the indole-diterpene, paspaline

scientific article published on 17 February 2006

Functional Crosstalk between Discrete Indole Terpenoid Gene Clusters in Tolypocladium album

scientific article published on 5 October 2023

Generation of Alternate Indole Diterpene Architectures in Two Species of Aspergilli

scientific article published on 30 January 2023

Heterologous Biosynthesis of Nodulisporic Acid F.

scientific article published on 28 December 2017

Hinge Twists and Population Shifts Deliver Regulated Catalysis for ATP-PRT in Histidine Biosynthesis

scientific article published on 09 April 2019

Identification of 4-amino-4-deoxychorismate synthase as the molecular target for the antimicrobial action of (6s)-6-fluoroshikimate

scientific article

Independent catalysis of the short form HisG from Lactococcus lactis.

scientific article published on 9 July 2016

Interdomain Conformational Changes Provide Allosteric Regulation En Route To Chorismate

scientific article published on 8 August 2016

Investigating the role of the hydroxyl groups of substrate erythrose 4-phosphate in the reaction catalysed by the first enzyme of the shikimate pathway

scientific article published on 16 September 2011

Key targets and relevant inhibitors for the drug discovery of tuberculosis

scientific article published on June 2013

MIDAS: A Modular DNA Assembly System for Synthetic Biology

scientific article published on 5 April 2018

Mechanistic divergence of two closely related aldol-like enzyme-catalysed reactions

scientific article published on 6 October 2005

Mechanistic studies on type I and type II dehydroquinase with (6 R )- and (6 S )-6-fluoro-3-dehydroquinic acids

scientific article published on 01 February 2000

Modifying the determinants of α-ketoacid substrate selectivity in mycobacterium tuberculosis α-isopropylmalate synthase

scientific article published on 5 March 2014

Molecular analysis of two cytochrome P450 monooxygenase genes required for paxilline biosynthesis in Penicillium paxilli, and effects of paxilline intermediates on mammalian maxi-K ion channels.

scientific article published on 18 July 2003

Molecular modeling studies of peptide inhibitors highlight the importance of conformational prearrangement for inhibition of calpain

scientific article published in July 2010

Neisseria meningitidisexpresses a single 3-deoxy-d-arabino-heptulosonate 7-phosphate synthase that is inhibited primarily by phenylalanine

scientific article

Nodulisporic acid E biosynthesis: in vivo characterisation of NodD1, an indole-diterpene prenyltransferase that acts on an emindole SB derived indole-diterpene scaffold

scientific article published on 27 May 2019

On the Temperature Dependence of Enzyme-Catalyzed Rates

scientific article published on 8 March 2016

Potent inhibitors of a shikimate pathway enzyme from Mycobacterium tuberculosis: combining mechanism- and modeling-based design

scientific article

Precise Positioning of Water Is Critical for Hydrolysis Catalyzed by 5′-Methylthioadenosine Nucleosidase

scientific article published on 15 August 2022

Probing the Sophisticated Synergistic Allosteric Regulation of Aromatic Amino Acid Biosynthesis in Mycobacterium tuberculosis Using ᴅ-Amino Acids

scientific article

Probing the determinants of phosphorylated sugar-substrate binding for human sialic acid synthase

scientific article published on 19 September 2014

Quaternary structure is an essential component that contributes to the sophisticated allosteric regulation mechanism in a key enzyme from Mycobacterium tuberculosis

scientific article published on 30 June 2017

Reactivity and mechanism in chemical and synthetic biology

scientific article published on 11 January 2023

Reciprocal allostery arising from a bi-enzyme assembly controls aromatic amino acid biosynthesis in Prevotella nigrescens

Reciprocal allostery arising from a bienzyme assembly controls aromatic amino acid biosynthesis in Prevotella nigrescens

scientific article published in September 2021

Removal of the C-terminal regulatory domain of α-isopropylmalate synthase disrupts functional substrate binding

scientific article

Reversing evolution: re-establishing obligate metal ion dependence in a metal-independent KDO8P synthase

scientific article

Selective Inhibition of Type II Dehydroquinases

scientific article published on 01 April 1999

Specificity and mutational analysis of the metal-dependent 3-deoxy-D-manno-octulosonate 8-phosphate synthase from Acidithiobacillus ferrooxidans

scientific article published on 18 April 2010

Stereospecific deuteration of 2-deoxyerythrose 4-phosphate using 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase

scientific article

Structural analysis of substrate-mimicking inhibitors in complex with Neisseria meningitidis 3-deoxy-d-arabino-heptulosonate 7-phosphate synthase - The importance of accommodating the active site water

scientific article

Structural and functional characterisation of the entry point to pyocyanin biosynthesis in Pseudomonas aeruginosa defines a new 3-deoxy-d-arabino-heptulosonate 7-phosphate synthase subclass.

scientific article published on 17 October 2018

Structure of the NDH-2 - HQNO inhibited complex provides molecular insight into quinone-binding site inhibitors

scientific article published on 2 April 2018

Structure, mechanism and inhibition of anthranilate phosphoribosyltransferase

scientific article published on 11 January 2023

Structures of Mycobacterium tuberculosis Anthranilate Phosphoribosyltransferase Variants Reveal the Conformational Changes That Facilitate Delivery of the Substrate to the Active Site

scientific article

Substrate ambiguity and crystal structure of Pyrococcus furiosus 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase: an ancestral 3-deoxyald-2-ulosonate-phosphate synthase?

scientific article

Substrate and reaction intermediate mimics as inhibitors of 3-deoxy-d-arabino-heptulosonate 7-phosphate synthase

Substrate deactivation of phenylalanine-sensitive 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase by erythrose 4-phosphate

scientific article published in December 2001

Substrate-mediated control of the conformation of an ancillary domain delivers a competent catalytic site for N-acetylneuraminic acid synthase

Synergistic Allostery, a Sophisticated Regulatory Network for the Control of Aromatic Amino Acid Biosynthesis in Mycobacterium tuberculosis

scientific article

Synthesis and evaluation of a mechanism-based inhibitor of a 3-deoxy-D-arabino heptulosonate 7-phosphate synthase.

scientific article

Synthesis and evaluation of dual site inhibitors of 3-deoxy-d-arabino-heptulosonate 7-phosphate synthase

scientific article published on 12 April 2011

Synthesis and evaluation of tetrahedral intermediate mimic inhibitors of 3-deoxy-d-manno-octulosonate 8-phosphate synthase

scientific article published on 10 December 2011

Targeting the role of a key conserved motif for substrate selection and catalysis by 3-deoxy-D-manno-octulosonate 8-phosphate synthase

scientific article

The C-terminal regulatory domain is required for catalysis by Neisseria meningitidis alpha-isopropylmalate synthase

scientific article published in February 2010

The Functional Unit of Neisseria meningitidis 3-Deoxy-ᴅ-Arabino-Heptulosonate 7-Phosphate Synthase Is Dimeric

scientific article published in February 2016

The Molecular Basis for the Substrate Specificity of Protein Tyrosine Phosphatase PTPN3

scientific article published on 01 April 2015

The genetic basis for indole-diterpene chemical diversity in filamentous fungi

scientific article published on 30 June 2007

The structure of 3-deoxy-d-arabino-heptulosonate 7-phosphate synthase from Mycobacterium tuberculosis reveals a common catalytic scaffold and ancestry for type I and type II enzymes

scientific journal article

The substrate capture mechanism of Mycobacterium tuberculosis anthranilate phosphoribosyltransferase provides a mode for inhibition

scientific article

Three sites and you are out: ternary synergistic allostery controls aromatic amino acid biosynthesis in Mycobacterium tuberculosis

scientific article

Transition State Analysis of Adenosine Triphosphate Phosphoribosyltransferase.

scientific article published on 5 September 2017

Tyrosine Latching of a Regulatory Gate Affords Allosteric Control of Aromatic Amino Acid Biosynthesis

scientific article

Using a Combination of Computational and Experimental Techniques to Understand the Molecular Basis for Protein Allostery

scientific article published on January 1, 2012

‘Tethering’ fragment-based drug discovery to identify inhibitors of the essential respiratory membrane protein type II NADH dehydrogenase

scientific article published on 26 May 2018

List of works by Emily Parker

A Pseudoisostructural Type II DAH7PS Enzyme from Pseudomonas aeruginosa: Alternative Evolutionary Strategies to Control Shikimate Pathway Flux

A dimeric catalytic core relates the short and long forms of ATP-phosphoribosyltransferase.

A single amino acid substitution uncouples catalysis and allostery in an essential biosynthetic enzyme in Mycobacterium tuberculosis

A symbiosis expressed non-ribosomal peptide synthetase from a mutualistic fungal endophyte of perennial ryegrass confers protection to the symbiotum from insect herbivory

Active site plasticity of a critical enzyme from Mycobacterium tuberculosis

Allosteric ACTion: the varied ACT domains regulating enzymes of amino-acid metabolism.

Allosteric inhibitor specificity of Thermotoga maritima 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase

Alternative substrates reveal catalytic cycle and key binding events in the reaction catalysed by anthranilate phosphoribosyltransferase from Mycobacterium tuberculosis

Amino Acid Biosynthesis

Amino-acid substitutions at the domain interface affect substrate and allosteric inhibitor binding in α-isopropylmalate synthase from Mycobacterium tuberculosis

Aminoacylation of Indole Diterpenes by Cluster-Specific Monomodular NRPS-like Enzymes

An extended β7α7 substrate-binding loop is essential for efficient catalysis by 3-deoxy-D-manno-octulosonate 8-phosphate synthase

Anthranilate phosphoribosyltransferase: Binding determinants for 5'-phospho-alpha-d-ribosyl-1'-pyrophosphate (PRPP) and the implications for inhibitor design.

Arabinose 5-phosphate analogues as mechanistic probes for Neisseria meningitidis 3-deoxy-D-manno-octulosonate 8-phosphate synthase

Arg314 is essential for catalysis by N-acetyl neuraminic acid synthase from Neisseria meningitidis

Biochemical and structural characterisation of dehydroquinate synthase from the New Zealand kiwifruit Actinidia chinensis

Biosynthesis of Nodulisporic Acids: A Multifunctional Monooxygenase Delivers a Complex and Highly Branched Array

Calculated pKa Variations Expose Dynamic Allosteric Communication Networks

Campylobacter jejuni adenosine triphosphate phosphoribosyltransferase is an active hexamer that is allosterically controlled by the twisting of a regulatory tail

Change in Heat Capacity for Enzyme Catalysis Determines Temperature Dependence of Enzyme Catalyzed Rates

Change in heat capacity for enzyme catalysis determines temperature dependence of enzyme catalyzed rates

Characterization of a recombinant type II 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase from Helicobacter pylori

Cloning and characterisation of dihydrodipicolinate synthase from the pathogen Neisseria meningitidis

Complex Formation between Two Biosynthetic Enzymes Modifies the Allosteric Regulatory Properties of Both: AN EXAMPLE OF MOLECULAR SYMBIOSIS.

Conformational interdomain flexibility in a bacterial α-isopropylmalate synthase is necessary for leucine biosynthesis

Crystal structure of type II NADH:quinone oxidoreductase from Caldalkalibacillus thermarum with an improved resolution of 2.15 Å.

Crystallization and preliminary X-ray crystallographic analysis of 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase from Mycobacterium tuberculosis

Datasets, processing and refinement details for Mtb-AnPRT: inhibitor structures with various space groups

Defining paxilline biosynthesis in Penicillium paxilli: functional characterization of two cytochrome P450 monooxygenases

Deletion and gene expression analyses define the paxilline biosynthetic gene cluster in Penicillium paxilli

Derailing Dehydroquinate Synthase by Introducing a Stabilizing Stereoelectronic Effect in a Reaction Intermediate ⬇️

Destabilization of the homotetrameric assembly of 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase from the hyperthermophile Pyrococcus furiosus enhances enzymatic activity

Diverse allosteric componentry and mechanisms control entry into aromatic metabolite biosynthesis

Domain cross-talk within a bifunctional enzyme provides catalytic and allosteric functionality in the biosynthesis of aromatic amino acids

Draft Genome Sequence of the Filamentous Fungus Hypoxylon pulicicidum ATCC 74245.

Dynamic cross-talk among remote binding sites: the molecular basis for unusual synergistic allostery

Editorial overview: Catalysis and regulation

Editorial overview: Catalysis and regulation: enzyme catalysis, biosynthetic pathways and regulation

Engineering allosteric control to an unregulated enzyme by transfer of a regulatory domain

Enzymatic synthesis of (6R)- and (6S)-fluoroshikimic acids

Enzymic synthesis of 3-[3- 13C]dehydroquinic acid

Erratum to “Reversing Evolution: Re-establishing Obligate Metal Ion Dependence in a Metal-independent KDO8P Synthase” [J. Mol. Biol. 390 (2009) 646–661]

Examining the role of intersubunit contacts in catalysis by 3-deoxy-d-manno-octulosonate 8-phosphate synthase

Exploring modular allostery via interchangeable regulatory domains

Expression, Purification, and Characterisation of Dehydroquinate Synthase from Pyrococcus furiosus

Expression, purification, and characterization of 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase from Pyrococcus furiosus

First structure of full-length mammalian phenylalanine hydroxylase reveals the architecture of an autoinhibited tetramer

Fluorinated substrates result in variable leakage of a reaction intermediate during catalysis by dehydroquinate synthase

Four gene products are required for the fungal synthesis of the indole-diterpene, paspaline

Functional Crosstalk between Discrete Indole Terpenoid Gene Clusters in <i>Tolypocladium album</i>

Generation of Alternate Indole Diterpene Architectures in Two Species of <i>Aspergilli</i>

Heterologous Biosynthesis of Nodulisporic Acid F.

Hinge Twists and Population Shifts Deliver Regulated Catalysis for ATP-PRT in Histidine Biosynthesis

Identification of 4-amino-4-deoxychorismate synthase as the molecular target for the antimicrobial action of (6s)-6-fluoroshikimate

Independent catalysis of the short form HisG from Lactococcus lactis.

Interdomain Conformational Changes Provide Allosteric Regulation En Route To Chorismate

Investigating the role of the hydroxyl groups of substrate erythrose 4-phosphate in the reaction catalysed by the first enzyme of the shikimate pathway

Key targets and relevant inhibitors for the drug discovery of tuberculosis

MIDAS: A Modular DNA Assembly System for Synthetic Biology

Mechanistic divergence of two closely related aldol-like enzyme-catalysed reactions

Mechanistic studies on type I and type II dehydroquinase with (6 R )- and (6 S )-6-fluoro-3-dehydroquinic acids

Modifying the determinants of α-ketoacid substrate selectivity in mycobacterium tuberculosis α-isopropylmalate synthase

Molecular analysis of two cytochrome P450 monooxygenase genes required for paxilline biosynthesis in Penicillium paxilli, and effects of paxilline intermediates on mammalian maxi-K ion channels.

Molecular modeling studies of peptide inhibitors highlight the importance of conformational prearrangement for inhibition of calpain

Neisseria meningitidisexpresses a single 3-deoxy-d-arabino-heptulosonate 7-phosphate synthase that is inhibited primarily by phenylalanine

Nodulisporic acid E biosynthesis: in vivo characterisation of NodD1, an indole-diterpene prenyltransferase that acts on an emindole SB derived indole-diterpene scaffold

On the Temperature Dependence of Enzyme-Catalyzed Rates

Potent inhibitors of a shikimate pathway enzyme from Mycobacterium tuberculosis: combining mechanism- and modeling-based design

Precise Positioning of Water Is Critical for Hydrolysis Catalyzed by 5′-Methylthioadenosine Nucleosidase

Probing the Sophisticated Synergistic Allosteric Regulation of Aromatic Amino Acid Biosynthesis in Mycobacterium tuberculosis Using ᴅ-Amino Acids

Probing the determinants of phosphorylated sugar-substrate binding for human sialic acid synthase

Quaternary structure is an essential component that contributes to the sophisticated allosteric regulation mechanism in a key enzyme from Mycobacterium tuberculosis

Reactivity and mechanism in chemical and synthetic biology

Reciprocal allostery arising from a bi-enzyme assembly controls aromatic amino acid biosynthesis in <i>Prevotella nigrescens</i>

Reciprocal allostery arising from a bienzyme assembly controls aromatic amino acid biosynthesis in Prevotella nigrescens

Removal of the C-terminal regulatory domain of α-isopropylmalate synthase disrupts functional substrate binding

Reversing evolution: re-establishing obligate metal ion dependence in a metal-independent KDO8P synthase

Selective Inhibition of Type II Dehydroquinases

Specificity and mutational analysis of the metal-dependent 3-deoxy-D-manno-octulosonate 8-phosphate synthase from Acidithiobacillus ferrooxidans