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List of works by David J Vocadlo

6''-Azido-6''-deoxy-UDP-N-acetylglucosamine as a glycosyltransferase substrate

scientific article published on 23 December 2010

A 1-acetamido derivative of 6-epi-valienamine: an inhibitor of a diverse group of beta-N-acetylglucosaminidases

scientific article

A Chemical Genetic Method for Monitoring Genome-Wide Dynamics of -GlcNAc Turnover on Chromatin-Associated Proteins

scientific article published on 01 March 2019

A Convenient Approach to Stereoisomeric Iminocyclitols: Generation of Potent Brain-Permeable OGA Inhibitors

scientific article

A Direct Fluorescent Activity Assay for Glycosyltransferases Enables Convenient High-Throughput Screening: Application to O-GlcNAc Transferase

scientific article published on 24 February 2020

A case for reverse protonation: identification of Glu160 as an acid/base catalyst in Thermoanaerobacterium saccharolyticum beta-xylosidase and detailed kinetic analysis of a site-directed mutant.

scientific article

A divergent synthesis of 2-acyl derivatives of PUGNAc yields selective inhibitors of O-GlcNAcase.

scientific article published on 18 January 2006

A highly concise preparation of O-deacetylated arylthioglycosides of N-acetyl-D-glucosamine from 2-acetamido-3,4,6-tri-O-acetyl-2-deoxy-alpha-D-glucopyranosyl chloride and aryl thiols or disulfides

scientific article published on 10 February 2006

A mechanism-based GlcNAc-inspired cyclophellitol inactivator of the peptidoglycan recycling enzyme NagZ reverses resistance to β-lactams in Pseudomonas aeruginosa

scientific article published on 04 September 2018

A mechanism-based inactivator of glycoside hydrolases involving formation of a transient non-classical carbocation

scientific article published on 05 December 2014

A potent mechanism-inspired O-GlcNAcase inhibitor that blocks phosphorylation of tau in vivo

scientific article

A selective inhibitor Gal-PUGNAc of human lysosomal beta-hexosaminidases modulates levels of the ganglioside GM2 in neuroblastoma cells.

scientific article

A strategy for functional proteomic analysis of glycosidase activity from cell lysates.

scientific article published in October 2004

AmpG inactivation restores susceptibility of pan-beta-lactam-resistant Pseudomonas aeruginosa clinical strains

scientific article

Analysis of PUGNAc and NAG-thiazoline as transition state analogues for human O-GlcNAcase: mechanistic and structural insights into inhibitor selectivity and transition state poise

scientific article

Aspartate 313 in the Streptomyces plicatus hexosaminidase plays a critical role in substrate-assisted catalysis by orienting the 2-acetamido group and stabilizing the transition state

scientific article

Carbohydrate Bis-acetal-Based Substrates as Tunable Fluorescence-Quenched Probes for Monitoring exo-Glycosidase Activity

scientific article published on 20 June 2017

Catalysis by hen egg-white lysozyme proceeds via a covalent intermediate

scientific article

Catalytic Promiscuity of O-GlcNAc Transferase Enables Unexpected Metabolic Engineering of Cytoplasmic Proteins with 2-Azido-2-deoxy-glucose

scientific article

Characterization of a beta-N-acetylhexosaminidase and a beta-N-acetylglucosaminidase/beta-glucosidase from Cellulomonas fimi

scientific article

Characterization of the Glu and Asp residues in the active site of human beta-hexosaminidase B

scientific article

Conformational Itinerary of Pseudomonas aeruginosa 1,6-Anhydro- N -acetylmuramic Acid Kinase during Its Catalytic Cycle

scientific article

Crystal structure of beta-D-xylosidase from Thermoanaerobacterium saccharolyticum, a family 39 glycoside hydrolase

scientific article

Crystal structure of the AmpR effector binding domain provides insight into the molecular regulation of inducible ampc beta-lactamase

scientific article

Crystallographic evidence for substrate-assisted catalysis in a bacterial beta-hexosaminidase

scientific article

Cura Annonae-Chemically Boosting Crop Yields Through Metabolic Feeding of a Plant Signaling Precursor

scientific article

Detailed comparative analysis of the catalytic mechanisms of beta-N-acetylglucosaminidases from families 3 and 20 of glycoside hydrolases.

scientific article published in September 2005

Developing inhibitors of glycan processing enzymes as tools for enabling glycobiology.

scientific article published on 18 July 2012

Differential Effects of an O-GlcNAcase Inhibitor on Tau Phosphorylation ⬇️

scientific article published on April 19, 2012

Differential Recognition and Hydrolysis of Host Carbohydrate Antigens by Streptococcus pneumoniae Family 98 Glycoside Hydrolases

scientific article

Direct one-step fluorescent labeling of O-GlcNAc modified proteins in live cells using metabolic intermediates

scientific article published on 22 October 2018

Discovery of MK-8719, a Potent O-GlcNAcase Inhibitor as a Potential Treatment for Tauopathies

scientific article published on 29 September 2019

Drosophila O-GlcNAc transferase (OGT) is encoded by the Polycomb group (PcG) gene, super sex combs (sxc).

scientific article published on 28 July 2009

Elevation of Global O-GlcNAc Levels in 3T3-L1 Adipocytes by Selective Inhibition of O-GlcNAcase Does Not Induce Insulin Resistance

scientific article

Elevation of Global O-GlcNAc in rodents using a selective O-GlcNAcase inhibitor does not cause insulin resistance or perturb glucohomeostasis

scientific article published on September 2010

Enzymatic characterization and inhibition of the nuclear variant of human O-GlcNAcase

scientific article published on 20 April 2009

Fluorescence-quenched substrates for live cell imaging of human glucocerebrosidase activity.

scientific article

Functional analysis of a group A streptococcal glycoside hydrolase Spy1600 from family 84 reveals it is a beta-N-acetylglucosaminidase and not a hyaluronidase

scientific article

Functional proteomic profiling of glycan-processing enzymes

scientific article published on January 2006

Genome-wide chemical mapping of O-GlcNAcylated proteins in Drosophila melanogaster

scientific article published on 5 December 2016

HCF-1 Is Cleaved in the Active Site of O-GlcNAc Transferase

scientific article

Hijacking a biosynthetic pathway yields a glycosyltransferase inhibitor within cells.

scientific article published on 23 January 2011

How to make a difference: mechanisms of protein and nucleic acid modifying enzymes

scientific article published on 16 November 2012

Hyper-O-GlcNAcylation is anti-apoptotic and maintains constitutive NF-κB activity in pancreatic cancer cells

scientific article

Identification of Glu-277 as the catalytic nucleophile of Thermoanaerobacterium saccharolyticum β-xylosidase using electrospray MS ⬇️

scientific article published on October 15, 1998

In vivo modulation of O-GlcNAc levels regulates hippocampal synaptic plasticity through interplay with phosphorylation.

scientific article published on 11 November 2008

Inactivation of the glycoside hydrolase NagZ attenuates antipseudomonal beta-lactam resistance in Pseudomonas aeruginosa

scientific article published on 9 March 2009

Increasing O-GlcNAc levels: An overview of small-molecule inhibitors of O-GlcNAcase.

scientific article published on 04 August 2009

Increasing O-GlcNAc slows neurodegeneration and stabilizes tau against aggregation.

scientific article published on 26 February 2012

Inhibition of O-GlcNAcase Using a Potent and Cell-Permeable Inhibitor Does Not Induce Insulin Resistance in 3T3-L1 Adipocytes

scientific article

Inhibition of O-GlcNAcase by a gluco-configured nagstatin and a PUGNAc–imidazole hybrid inhibitor

scientific article published on 29 September 2006

Inhibition of the family 20 glycoside hydrolase catalytic modules in the Streptococcus pneumoniae exo-β-D-N-acetylglucosaminidase, StrH

scientific article

Inhibition of the pneumococcal virulence factor StrH and molecular insights into N-glycan recognition and hydrolysis

scientific article published on 9 November 2011

Insight into a strategy for attenuating AmpC-mediated β-lactam resistance: Structural basis for selective inhibition of the glycoside hydrolase NagZ

scientific article

Insights into O-linked N-acetylglucosamine ([0-9]O-GlcNAc) processing and dynamics through kinetic analysis of O-GlcNAc transferase and O-GlcNAcase activity on protein substrates.

scientific article

Mammalian Notch is modified by D-Xyl-alpha1-3-D-Xyl-alpha1-3-D-Glc-beta1-O-Ser: implementation of a method to study O-glucosylation

scientific article published on 11 November 2009

Mapping O-GlcNAc modification sites on tau and generation of a site-specific O-GlcNAc tau antibody

scientific article published on 13 August 2010

Mechanism of action and identification of Asp242 as the catalytic nucleophile of Vibrio furnisii N-acetyl-beta-D-glucosaminidase using 2-acetamido-2-deoxy-5-fluoro-alpha-L-idopyranosyl fluoride

scientific article

Mechanistic insights into glycosidase chemistry

scientific article published on October 2008

Metabolic Inhibitors of O-GlcNAc Transferase That Act In Vivo Implicate Decreased O-GlcNAc Levels in Leptin-Mediated Nutrient Sensing

scientific article published on 24 May 2018

Metabolic inhibition of sialyl-Lewis X biosynthesis by 5-thiofucose remodels the cell surface and impairs selectin-mediated cell adhesion.

scientific article published on 27 September 2012

Metabolism of Vertebrate Amino Sugars with N-Glycolyl Groups: INTRACELLULAR  -O-LINKED N-GLYCOLYLGLUCOSAMINE (GlcNGc), UDP-GlcNGc, AND THE BIOCHEMICAL AND STRUCTURAL RATIONALE FOR THE SUBSTRATE TOLERANCE OF  -O-LINKED  -N-ACETYLGLUCOSAMINIDASE

scientific article

Metabolism of vertebrate amino sugars with N-glycolyl groups: incorporation of N-glycolylhexosamines into mammalian glycans by feeding N-glycolylgalactosamine

scientific article

Mislocalization of TDP-43 in the G93A mutant SOD1 transgenic mouse model of ALS.

scientific article published on 18 April 2009

Molecular Basis of 1,6-Anhydro Bond Cleavage and Phosphoryl Transfer by Pseudomonas aeruginosa 1,6-Anhydro-N-acetylmuramic Acid Kinase

scientific article

Molecular basis for G protein control of the prokaryotic ATP sulfurylase

scientific article published in January 2006

Molecular basis for inhibition of GH84 glycoside hydrolases by substituted azepanes: conformational flexibility enables probing of substrate distortion

scientific article

NagZ inactivation prevents and reverts beta-lactam resistance, driven by AmpD and PBP 4 mutations, in Pseudomonas aeruginosa

scientific article published on 21 June 2010

O-GLcNAc post-translational modifications regulate the entry of neurons into an axon branching program

scientific article published on February 2009

O-GlcNAc and neurodegeneration: biochemical mechanisms and potential roles in Alzheimer's disease and beyond.

scientific article published on 24 April 2014

O-GlcNAc modification of tau directly inhibits its aggregation without perturbing the conformational properties of tau monomers

scientific article published on 18 January 2014

O-GlcNAc occurs cotranslationally to stabilize nascent polypeptide chains

scientific article published on 16 March 2015

O-GlcNAc processing enzymes: catalytic mechanisms, substrate specificity, and enzyme regulation.

scientific article

O-GlcNAcase uses substrate-assisted catalysis: kinetic analysis and development of highly selective mechanism-inspired inhibitors.

scientific article published on 28 March 2005

O-GlcNAcylation regulates cancer metabolism and survival stress signaling via regulation of the HIF-1 pathway.

scientific article

Pharmacological inhibition and knockdown of O-GlcNAcase reduces cellular internalization of α-synuclein pre-formed-fibrils

scientific article published on 04 May 2020

Pharmacological inhibition of O-GlcNAcase (OGA) enhances autophagy in brain through an mTOR-independent pathway.

scientific article published on 20 February 2018

Pharmacological inhibition of O-GlcNAcase (OGA) prevents cognitive decline and amyloid plaque formation in bigenic tau/APP mutant mice.

scientific article

Post-translational O-GlcNAcylation is essential for nuclear pore integrity and maintenance of the pore selectivity filter

scientific article

Probing synergy between two catalytic strategies in the glycoside hydrolase O-GlcNAcase using multiple linear free energy relationships

scientific article published in September 2009

Production of α-L-iduronidase in maize for the potential treatment of a human lysosomal storage disease

scientific article published in Nature Communications

Providing β-lactams a helping hand: targeting the AmpC β-lactamase induction pathway.

scientific article published on December 2011

Reduced protein O-glycosylation in the nervous system of the mutant SOD1 transgenic mouse model of amyotrophic lateral sclerosis

scientific article published on 12 April 2012

Role of beta Arg211 in the active site of human beta-hexosaminidase B.

scientific article published on May 2000

Selective Fluorogenic β-Glucocerebrosidase Substrates for Convenient Analysis of Enzyme Activity in Cell and Tissue Homogenates

scientific article published on 16 March 2020

Selective trihydroxyazepane NagZ inhibitors increase sensitivity of Pseudomonas aeruginosa to β-lactams

scientific article

Software for rapid time dependent ChIP-sequencing analysis (TDCA).

scientific article published on 25 November 2017

Streptococcus pneumoniae endohexosaminidase D; feasibility of using N-glycan oxazoline donors for synthetic glycosylation of a GlcNAc-asparagine acceptor

scientific article published on April 2010

Structural Analysis of a Family 101 Glycoside Hydrolase in Complex with Carbohydrates Reveals Insights into Its Mechanism

scientific article

Structural and functional insight into human O-GlcNAcase ⬇️

scientific article published on 27 March 2017

Structural snapshots of the reaction coordinate for O-GlcNAc transferase

scientific article

Structural, mechanistic, and computational analysis of the effects of anomeric fluorines on anomeric fluoride departure in 5-fluoroxylosyl fluorides

scientific article published on 19 September 2011

Structure of an O-GlcNAc transferase homolog provides insight into intracellular glycosylation

scientific article

Structures of lactate dehydrogenase A (LDHA) in apo, ternary and inhibitor-bound forms

scientific article

Substrate-guided front-face reaction revealed by combined structural snapshots and metadynamics for the polypeptide N-acetylgalactosaminyltransferase 2

scientific article

Synthesis of 4-methylumbelliferyl α-d-mannopyranosyl-(1→6)-β-d-mannopyranoside and development of a coupled fluorescent assay for GH125 exo-α-1,6-mannosidases.

scientific article

Tandem Bioorthogonal Labeling Uncovers Endogenous Cotranslationally O-GlcNAc Modified Nascent Proteins

scientific article published on 01 September 2020

The Development of Selective Inhibitors of NagZ: Increased Susceptibility of Gram-Negative Bacteria to β-Lactams

scientific article

The chemical synthesis of 2-deoxy-2-fluorodisaccharide probes of the hen egg white lysozyme mechanism.

scientific article

The conformation and function of a multimodular glycogen-degrading pneumococcal virulence factor

scientific article

The emerging link between O-GlcNAc and Alzheimer disease.

scientific article

The nutrient sensor OGT regulates Hipk stability and tumorigenic-like activities in Drosophila

scientific article published on 13 January 2020

The β-lactamase gene regulator AmpR is a tetramer that recognizes and binds the D-Ala-D-Ala motif of its repressor UDP-N-acetylmuramic acid (MurNAc)-pentapeptide

scientific article

Visualizing the reaction coordinate of an O-GlcNAc hydrolase

scientific article

mTOR/MYC Axis Regulates O-GlcNAc Transferase Expression and O-GlcNAcylation in Breast Cancer.

scientific article published on 30 January 2015

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