List of works - Jim Huggett

A comparison of miRNA isolation and RT-qPCR technologies and their effects on quantification accuracy and repeatability

scientific article published in March 2013

A novel approach for evaluating the performance of real time quantitative loop-mediated isothermal amplification-based methods.

scientific article

Accurate and rapid identification of bacterial species from positive blood cultures with a DNA-based microarray platform: an observational study

scientific article published on 10 December 2009

An international comparability study on quantification of mRNA gene expression ratios: CCQM-P103.1.

scientific article published on 06 June 2016

Application of next generation qPCR and sequencing platforms to mRNA biomarker analysis

scientific article

Assessing the accuracy of quantitative molecular microbial profiling

scientific article

Assessment of Digital PCR as a Primary Reference Measurement Procedure to Support Advances in Precision Medicine

scientific article published on 14 June 2018

Chemical mixtures and fluorescence in situ hybridization analysis of natural microbial community in the Tiber river

scientific article published on 03 April 2019

Clinical features, microbiology and surgical outcomes of infective endocarditis: a 13-year study from a UK tertiary cardiothoracic referral centre.

scientific article published on 15 September 2014

Comparative study of sensitivity, linearity, and resistance to inhibition of digital and nondigital polymerase chain reaction and loop mediated isothermal amplification assays for quantification of human cytomegalovirus.

scientific article published on 22 April 2014

Comparison of microfluidic digital PCR and conventional quantitative PCR for measuring copy number variation

scientific article

Considerations for accurate gene expression measurement by reverse transcription quantitative PCR when analysing clinical samples

scientific article

Considerations for digital PCR as an accurate molecular diagnostic tool.

scientific article

Crohn's disease and MAP.

scientific article

Detection of Rare Drug Resistance Mutations by Digital PCR in a Human Influenza A Virus Model System and Clinical Samples.

scientific article published on 9 December 2015

Development of a highly sensitive liquid biopsy platform to detect clinically-relevant cancer mutations at low allele fractions in cell-free DNA.

scientific article published on 16 March 2018

Different screening strategies (single or dual) for the diagnosis of suspected latent tuberculosis: a cost effectiveness analysis

scientific article

Differential susceptibility of PCR reactions to inhibitors: an important and unrecognised phenomenon

scientific article

Digital PCR as a novel technology and its potential implications for molecular diagnostics

scientific article published on 7 October 2013

Digital PCR, a technique for the future

scientific article published on 25 November 2016

Discordant bioinformatic predictions of antimicrobial resistance from whole-genome sequencing data of bacterial isolates: an inter-laboratory study

scientific article published on 12 February 2020

Evaluation of digital PCR for absolute DNA quantification

scientific article

Evaluation of digital PCR for absolute RNA quantification

scientific article

Expression of a novel cytokine, IL-4delta2, in HIV and HIV-tuberculosis co-infection.

scientific article

Expression of apoptosis-related genes in an Ethiopian cohort study correlates with tuberculosis clinical status

scientific article published in January 2010

Fundamentals of multiplexing with digital PCR

scientific article

Future diagnosis of sepsis – Authors' reply

scientific article published in The Lancet

G6PD deficiency alleles in a malaria-endemic region in the Western Brazilian Amazon

scientific article

Highly reproducible absolute quantification of Mycobacterium tuberculosis complex by digital PCR.

scientific article published on 10 March 2015

How to make Mathematics Biology's next and better microscope.

scientific article published on 10 September 2014

Implications of storing urinary DNA from different populations for molecular analyses

scientific article

Improving the standardization of mRNA measurement by RT-qPCR.

scientific article

In vivo and in vitro studies of a novel cytokine, interleukin 4delta2, in pulmonary tuberculosis

scientific article

Instability of 8E5 calibration standard revealed by digital PCR risks inaccurate quantification of HIV DNA in clinical samples by qPCR.

scientific article

Integrating informatics tools and portable sequencing technology for rapid detection of resistance to anti-tuberculous drugs

scientific article published on 24 June 2019

Inter-laboratory assessment of different digital PCR platforms for quantification of human cytomegalovirus DNA.

scientific article published on 26 January 2017

International Comparison of Enumeration-Based Quantification of DNA Copy-Concentration Using Flow Cytometric Counting and Digital Polymerase Chain Reaction.

scientific article

International Interlaboratory Digital PCR Study Demonstrating High Reproducibility for the Measurement of a Rare Sequence Variant

scientific article published on 9 December 2016

International interlaboratory study comparing single organism 16S rRNA gene sequencing data: Beyond consensus sequence comparisons

scientific article

Low sensitivity of a urine LAM-ELISA in the diagnosis of pulmonary tuberculosis

scientific article published on 28 August 2009

Lung remodeling in pulmonary tuberculosis

scientific article

MIQE précis: Practical implementation of minimum standard guidelines for fluorescence-based quantitative real-time PCR experiments

scientific article published on 21 September 2010

Making standards for quantitative real-time pneumococcal PCR.

scientific article published on 04 December 2014

Methods for applying accurate digital PCR analysis on low copy DNA samples

scientific article

Minimum information necessary for quantitative real-time PCR experiments.

scientific article published in January 2014

Myobacterium tuberculosis induces selective up-regulation of TLRs in the mononuclear leukocytes of patients with active pulmonary tuberculosis

scientific article published on March 2006

Next-Generation Sequencing-Assisted DNA-Based Digital PCR for a Personalized Approach to the Detection and Quantification of Residual Disease in Chronic Myeloid Leukemia Patients.

scientific article published on 30 January 2016

Nucleic acid detection and quantification in the developing world

scientific article published on April 2009

Phage display of functional αβ single-chain T-cell receptor molecules specific for CD1b:Ac₂SGL complexes from Mycobacterium tuberculosis-infected cells

scientific article

Pneumocystis jirovecii in pleural infection: a nucleic acid amplification study

scientific article published on 30 August 2010

Primer sequence disclosure: a clarification of the MIQE guidelines.

scientific article

Problems of developing molecular diagnostic tests for opportunistic pathogens: the example of Pneumocystis jirovecii.

scientific article

Progress in metagenomics requires a balanced appraisal of the available technologies.

scientific article

Quality assessment of biobanked nucleic acid extracts for downstream molecular analysis

scientific article published in June 2012

Quantification of epigenetic biomarkers: an evaluation of established and emerging methods for DNA methylation analysis

scientific article published on 23 December 2014

Quantitative analysis of human endogenous retrovirus-K transcripts in postmortem premotor cortex fails to confirm elevated expression of HERV-K RNA in amyotrophic lateral sclerosis

scientific article published on 18 March 2019

RNA reference materials with defined viral RNA loads of SARS-CoV-2-A useful tool towards a better PCR assay harmonization

scientific article published on 20 January 2022

RT-qPCR and RT-Digital PCR: A Comparison of Different Platforms for the Evaluation of Residual Disease in Chronic Myeloid Leukemia

scientific article

Rapid and accurate detection of Mycobacterium tuberculosis in sputum samples by Cepheid Xpert MTB/RIF assay--a clinical validation study

scientific article

Rapid diagnosis of tuberculosis through the detection of mycobacterial DNA in urine by nucleic acid amplification methods

scientific article published on August 2009

Real-time RT-PCR normalisation; strategies and considerations.

scientific article

Reflections on the white plague

scientific article

Report of the 2019 NIST-FDA workshop on standards for next generation sequencing detection of viral adventitious agents in biologics and biomanufacturing

scientific article published on 22 February 2020

Reproducibility of biomedical research - The importance of editorial vigilance

scientific article published on 21 February 2017

Response to the Letter from Garcia-Montojo and colleagues concerning our paper entitled, Quantitative analysis of human endogenous retrovirus-K transcripts in postmortem premotor cortex fails to confirm elevated expression of HERV-K RNA in amyotroph

scientific article published on 03 July 2019

Seasonal variation in mortality of Pneumocystis jirovecii pneumonia in HIV-infected patients

article

Selection of phage-displayed human antibody fragments specific for CD1b presenting the Mycobacterium tuberculosis glycolipid Ac2SGL.

scientific article

Standardization of Nucleic Acid Tests for Clinical Measurements of Bacteria and Viruses.

scientific article published on 12 November 2014

The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments

scientific article (publication date: April 2009)

The digital MIQE guidelines: Minimum Information for Publication of Quantitative Digital PCR Experiments

scientific article

The need for transparency and good practices in the qPCR literature.

scientific article published in November 2013

The open reading frame of the Na(+)-dependent glutamate transporter GLAST-1 is expressed in bone and a splice variant of this molecule is expressed in bone and brain.

scientific article

The pathogen recognition sensor, NOD2, is variably expressed in patients with pulmonary tuberculosis

scientific article

The use of digital PCR to improve the application of quantitative molecular diagnostic methods for tuberculosis

scientific article

Towards standardisation of cell-free DNA measurement in plasma: controls for extraction efficiency, fragment size bias and quantification

scientific article

Trials and tribulations of an African-led research and capacity development programme: the case for EDCTP investments

scientific article published on 17 February 2010

Tuberculosis: amplification-based clinical diagnostic techniques

scientific article

Type 2 cytokines in respiratory syncytial virus bronchiolitis

scientific article published in May 2004

Unreliable real-time PCR analysis of human endogenous retrovirus-W (HERV-W) RNA expression and DNA copy number in multiple sclerosis.

scientific article

Utility of the antigen-specific interferon-gamma assay for the management of tuberculosis

scientific article

Variation in gamma interferon responses to different infecting strains of Mycobacterium tuberculosis in acid-fast bacillus smear-positive patients and household contacts in Antananarivo, Madagascar

scientific article

qPCR primer design revisited

scientific article published on 22 November 2017

qPCR, dPCR, NGS - A journey.

scientific article published on 15 January 2015

List of works by Jim Huggett

A comparison of miRNA isolation and RT-qPCR technologies and their effects on quantification accuracy and repeatability

A novel approach for evaluating the performance of real time quantitative loop-mediated isothermal amplification-based methods.

Accurate and rapid identification of bacterial species from positive blood cultures with a DNA-based microarray platform: an observational study

An international comparability study on quantification of mRNA gene expression ratios: CCQM-P103.1.

Application of next generation qPCR and sequencing platforms to mRNA biomarker analysis

Assessing the accuracy of quantitative molecular microbial profiling

Assessment of Digital PCR as a Primary Reference Measurement Procedure to Support Advances in Precision Medicine

Chemical mixtures and fluorescence in situ hybridization analysis of natural microbial community in the Tiber river

Clinical features, microbiology and surgical outcomes of infective endocarditis: a 13-year study from a UK tertiary cardiothoracic referral centre.

Comparative study of sensitivity, linearity, and resistance to inhibition of digital and nondigital polymerase chain reaction and loop mediated isothermal amplification assays for quantification of human cytomegalovirus.

Comparison of microfluidic digital PCR and conventional quantitative PCR for measuring copy number variation

Considerations for accurate gene expression measurement by reverse transcription quantitative PCR when analysing clinical samples

Considerations for digital PCR as an accurate molecular diagnostic tool.

Crohn's disease and MAP.

Detection of Rare Drug Resistance Mutations by Digital PCR in a Human Influenza A Virus Model System and Clinical Samples.

Development of a highly sensitive liquid biopsy platform to detect clinically-relevant cancer mutations at low allele fractions in cell-free DNA.

Different screening strategies (single or dual) for the diagnosis of suspected latent tuberculosis: a cost effectiveness analysis

Differential susceptibility of PCR reactions to inhibitors: an important and unrecognised phenomenon

Digital PCR as a novel technology and its potential implications for molecular diagnostics

Digital PCR, a technique for the future

Discordant bioinformatic predictions of antimicrobial resistance from whole-genome sequencing data of bacterial isolates: an inter-laboratory study

Evaluation of digital PCR for absolute DNA quantification

Evaluation of digital PCR for absolute RNA quantification

Expression of a novel cytokine, IL-4delta2, in HIV and HIV-tuberculosis co-infection.

Expression of apoptosis-related genes in an Ethiopian cohort study correlates with tuberculosis clinical status

Fundamentals of multiplexing with digital PCR

Future diagnosis of sepsis – Authors' reply

G6PD deficiency alleles in a malaria-endemic region in the Western Brazilian Amazon

Highly reproducible absolute quantification of Mycobacterium tuberculosis complex by digital PCR.

How to make Mathematics Biology's next and better microscope.

Implications of storing urinary DNA from different populations for molecular analyses

Improving the standardization of mRNA measurement by RT-qPCR.

In vivo and in vitro studies of a novel cytokine, interleukin 4delta2, in pulmonary tuberculosis

Instability of 8E5 calibration standard revealed by digital PCR risks inaccurate quantification of HIV DNA in clinical samples by qPCR.

Integrating informatics tools and portable sequencing technology for rapid detection of resistance to anti-tuberculous drugs

Inter-laboratory assessment of different digital PCR platforms for quantification of human cytomegalovirus DNA.

International Comparison of Enumeration-Based Quantification of DNA Copy-Concentration Using Flow Cytometric Counting and Digital Polymerase Chain Reaction.

International Interlaboratory Digital PCR Study Demonstrating High Reproducibility for the Measurement of a Rare Sequence Variant

International interlaboratory study comparing single organism 16S rRNA gene sequencing data: Beyond consensus sequence comparisons

Low sensitivity of a urine LAM-ELISA in the diagnosis of pulmonary tuberculosis

Lung remodeling in pulmonary tuberculosis

MIQE précis: Practical implementation of minimum standard guidelines for fluorescence-based quantitative real-time PCR experiments

Making standards for quantitative real-time pneumococcal PCR.

Methods for applying accurate digital PCR analysis on low copy DNA samples

Minimum information necessary for quantitative real-time PCR experiments.

Myobacterium tuberculosis induces selective up-regulation of TLRs in the mononuclear leukocytes of patients with active pulmonary tuberculosis

Next-Generation Sequencing-Assisted DNA-Based Digital PCR for a Personalized Approach to the Detection and Quantification of Residual Disease in Chronic Myeloid Leukemia Patients.

Nucleic acid detection and quantification in the developing world

Phage display of functional αβ single-chain T-cell receptor molecules specific for CD1b:Ac₂SGL complexes from Mycobacterium tuberculosis-infected cells

Pneumocystis jirovecii in pleural infection: a nucleic acid amplification study

Primer sequence disclosure: a clarification of the MIQE guidelines.

Problems of developing molecular diagnostic tests for opportunistic pathogens: the example of Pneumocystis jirovecii.

Progress in metagenomics requires a balanced appraisal of the available technologies.

Quality assessment of biobanked nucleic acid extracts for downstream molecular analysis

Quantification of epigenetic biomarkers: an evaluation of established and emerging methods for DNA methylation analysis

Quantitative analysis of human endogenous retrovirus-K transcripts in postmortem premotor cortex fails to confirm elevated expression of HERV-K RNA in amyotrophic lateral sclerosis

RNA reference materials with defined viral RNA loads of SARS-CoV-2-A useful tool towards a better PCR assay harmonization

RT-qPCR and RT-Digital PCR: A Comparison of Different Platforms for the Evaluation of Residual Disease in Chronic Myeloid Leukemia

Rapid and accurate detection of Mycobacterium tuberculosis in sputum samples by Cepheid Xpert MTB/RIF assay--a clinical validation study

Rapid diagnosis of tuberculosis through the detection of mycobacterial DNA in urine by nucleic acid amplification methods

Real-time RT-PCR normalisation; strategies and considerations.

Reflections on the white plague

Report of the 2019 NIST-FDA workshop on standards for next generation sequencing detection of viral adventitious agents in biologics and biomanufacturing

Reproducibility of biomedical research - The importance of editorial vigilance

Response to the Letter from Garcia-Montojo and colleagues concerning our paper entitled, Quantitative analysis of human endogenous retrovirus-K transcripts in postmortem premotor cortex fails to confirm elevated expression of HERV-K RNA in amyotroph

Seasonal variation in mortality of Pneumocystis jirovecii pneumonia in HIV-infected patients

Selection of phage-displayed human antibody fragments specific for CD1b presenting the Mycobacterium tuberculosis glycolipid Ac2SGL.

Standardization of Nucleic Acid Tests for Clinical Measurements of Bacteria and Viruses.

The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments

The digital MIQE guidelines: Minimum Information for Publication of Quantitative Digital PCR Experiments

The need for transparency and good practices in the qPCR literature.

The open reading frame of the Na(+)-dependent glutamate transporter GLAST-1 is expressed in bone and a splice variant of this molecule is expressed in bone and brain.

The pathogen recognition sensor, NOD2, is variably expressed in patients with pulmonary tuberculosis

The use of digital PCR to improve the application of quantitative molecular diagnostic methods for tuberculosis

Towards standardisation of cell-free DNA measurement in plasma: controls for extraction efficiency, fragment size bias and quantification

Trials and tribulations of an African-led research and capacity development programme: the case for EDCTP investments

Tuberculosis: amplification-based clinical diagnostic techniques

Type 2 cytokines in respiratory syncytial virus bronchiolitis

Unreliable real-time PCR analysis of human endogenous retrovirus-W (HERV-W) RNA expression and DNA copy number in multiple sclerosis.