List of works - Frank Sargent

A Novel Aerobic Mechanism for Reductive Palladium Biomineralization and Recovery byEscherichia coli

A genetic analysis of in vivo selenate reduction by Salmonella enterica serovar Typhimurium LT2 and Escherichia coli K12.

scientific article published on 5 May 2009

A genetic screen for suppressors of Escherichia coli Tat signal peptide mutations establishes a critical role for the second arginine within the twin-arginine motif

scientific article published on 30 October 2001

A holin and an endopeptidase are essential for chitinolytic protein secretion in Serratia marcescens

scientific article

A holin/peptidoglycan hydrolase-dependent protein secretion system

scientific article published on 04 September 2020

A marriage of bacteriology with cell biology results in twin arginines

scientific article

A novel protein transport system involved in the biogenesis of bacterial electron transfer chains

article published in 2000

A regulatory domain controls the transport activity of a twin-arginine signal peptide

scientific article published on 10 September 2013

A subset of bacterial inner membrane proteins integrated by the twin-arginine translocase

scientific article published in September 2003

A synthetic system for expression of components of a bacterial microcompartment

scientific article published on 06 September 2013

An essential component of a novel bacterial protein export system with homologues in plastids and mitochondria

scientific article published in July 1998

Analysis of Tat targeting function and twin-arginine signal peptide activity in Escherichia coli

scientific article published in January 2010

Analysis of hydrogenase 1 levels reveals an intimate link between carbon and hydrogen metabolism in Escherichia coli K-12.

scientific article

Assembly of Tat-dependent [NiFe] hydrogenases: identification of precursor-binding accessory proteins

scientific article published in August 2003

Assembly of membrane-bound respiratory complexes by the Tat protein-transport system

scientific article

Bacterial formate hydrogenlyase complex

scientific article

Behaviour of topological marker proteins targeted to the Tat protein transport pathway

scientific article

Biorecovery of Precious Metals from Wastes and Conversion into Fuel Cell Catalyst for Electricity Production

Biosynthesis of Salmonella enterica [NiFe]-hydrogenase-5: probing the roles of system-specific accessory proteins

scientific article published on 26 August 2016

Biosynthesis of selenate reductase in Salmonella enterica: critical roles for the signal peptide and DmsD.

scientific article published on 20 October 2016

Biosynthesis of the respiratory formate dehydrogenases from Escherichia coli: characterization of the FdhE protein

scientific article

Chaperones involved in assembly and export of N-oxide reductases

scientific article published in February 2005

Characterisation of the membrane-extrinsic domain of the TatB component of the twin arginine protein translocase.

scientific article published on 13 January 2011

Characterization of a periplasmic nitrate reductase in complex with its biosynthetic chaperone

scientific article published on 9 December 2013

Characterization of a pre-export enzyme-chaperone complex on the twin-arginine transport pathway

scientific article published on May 2013

Common principles in the biosynthesis of diverse enzymes.

scientific article published in February 2005

Conserved Signal Peptide Recognition Systems across the Prokaryotic Domains

scientific article

Constitutive expression of Escherichia coli tat genes indicates an important role for the twin-arginine translocase during aerobic and anaerobic growth

scientific article

Constructing the wonders of the bacterial world: biosynthesis of complex enzymes

scientific article published on March 2007

Controlling and co-ordinating chitinase secretion in a Serratia marcescens population

scientific article published on 01 November 2019

Coordinating assembly and export of complex bacterial proteins

scientific article published on 23 September 2004

Crystal structure of the O(2)-tolerant membrane-bound hydrogenase 1 from Escherichia coli in complex with its cognate cytochrome b

scientific article

Crystal structure of the molybdenum cofactor biosynthesis protein MobA from Escherichia coli at near-atomic resolution

scientific article

Design and characterisation of synthetic operons for biohydrogen technology

scientific article published on 21 November 2016

Dissecting the roles of Escherichia coli hydrogenases in biohydrogen production

scientific article published on 6 November 2007

Dissection and engineering of the Escherichia coli formate hydrogenlyase complex

scientific article published on 7 September 2015

Efficient Hydrogen-Dependent Carbon Dioxide Reduction by Escherichia coli.

scientific article published on 20 December 2017

Efficient electron transfer from hydrogen to benzyl viologen by the [NiFe]-hydrogenases of Escherichia coli is dependent on the coexpression of the iron-sulfur cluster-containing small subunit

scientific article

Escherichia coli tat mutant strains are able to transport maltose in the absence of an active malE gene

scientific article

Exploring the directionality of Escherichia coli formate hydrogenlyase: a membrane-bound enzyme capable of fixing carbon dioxide to organic acid

scientific article published on 2 May 2016

Export of complex cofactor-containing proteins by the bacterial Tat pathway

scientific article

Features of a twin-arginine signal peptide required for recognition by a Tat proofreading chaperone

scientific article

Functional complexity of the twin-arginine translocase TatC component revealed by site-directed mutagenesis

scientific article published in March 2002

Harnessing Escherichia coli for Bio-Based Production of Formate under Pressurized H<sub>2</sub> and CO<sub>2</sub> Gases

scientific article published on 08 September 2021

How Escherichia coli is equipped to oxidize hydrogen under different redox conditions

scientific article

How Escherichia coli is equipped to oxidize hydrogen under different redox conditions

scientific article published in Journal of Biological Chemistry

How Salmonella oxidises H(2) under aerobic conditions

scientific article published on 5 August 2011

How oxygen reacts with oxygen-tolerant respiratory [NiFe]-hydrogenases

scientific article

How the oxygen tolerance of a [NiFe]-hydrogenase depends on quaternary structure

scientific article published on 09 February 2016

How the structure of the large subunit controls function in an oxygen-tolerant [NiFe]-hydrogenase

scientific article

Hydrogen production in the presence of oxygen by Escherichia coli K-12

scientific article published in 2022

Identification of a stable complex between a [NiFe]-hydrogenase catalytic subunit and its maturation protease.

scientific article published on 28 December 2016

Inactivation of the Escherichia coli K-12 twin-arginine translocation system promotes increased hydrogen production

scientific article published in September 2006

Integration of an [FeFe]-hydrogenase into the anaerobic metabolism of Escherichia coli.

scientific article

Intrinsic GTPase activity of a bacterial twin-arginine translocation proofreading chaperone induced by domain swapping

scientific article published in January 2010

Involvement of hydrogenases in the formation of highly catalytic Pd(0) nanoparticles by bioreduction of Pd(II) using Escherichia coli mutant strains

scientific article published on 11 June 2010

Light traffic: photo-crosslinking a novel transport system

scientific article published in February 2004

Look on the positive side! The orientation, identification and bioenergetics of 'Archaeal' membrane-bound nitrate reductases

scientific article published on 20 September 2007

Mechanism of hydrogen activation by [NiFe] hydrogenases

scientific article

Membrane interactions and self-association of the TatA and TatB components of the twin-arginine translocation pathway

scientific article published on 01 October 2001

Oligomeric properties and signal peptide binding by Escherichia coli Tat protein transport complexes.

scientific article published in October 2002

Overlapping functions of components of a bacterial Sec-independent protein export pathway

scientific article

Overlapping transport and chaperone-binding functions within a bacterial twin-arginine signal peptide

scientific article published on 27 February 2012

Oxygen-tolerant [NiFe]-hydrogenases: the individual and collective importance of supernumerary cysteines at the proximal Fe-S cluster

scientific article published on 4 October 2011

Pathfinders and trailblazers: a prokaryotic targeting system for transport of folded proteins

scientific article

Physiology and bioenergetics of [NiFe]-hydrogenase 2-catalyzed H2-consuming and H2-producing reactions in Escherichia coli

scientific article

Principles of sustained enzymatic hydrogen oxidation in the presence of oxygen--the crucial influence of high potential Fe-S clusters in the electron relay of [NiFe]-hydrogenases.

scientific article published on 11 February 2013

Protein targeting by the bacterial twin-arginine translocation (Tat) pathway

scientific article

Proteolytic processing of Escherichia coli twin-arginine signal peptides by LepB.

scientific article published on 7 October 2009

Purified components of the Escherichia coli Tat protein transport system form a double-layered ring structure

scientific article published in June 2001

Regulation of the hydrogenase-4 operon of Escherichia coli by the sigma(54)-dependent transcriptional activators FhlA and HyfR.

scientific article published on December 2002

Remnant signal peptides on non-exported enzymes: implications for the evolution of prokaryotic respiratory chains

scientific article

Sec-independent protein translocation in Escherichia coli. A distinct and pivotal role for the TatB protein

scientific article published in December 1999

Sequence analysis of bacterial redox enzyme maturation proteins (REMPs).

scientific article published in April 2004

Signal peptide etiquette during assembly of a complex respiratory enzyme

scientific article published on 8 September 2013

Signal peptide-chaperone interactions on the twin-arginine protein transport pathway.

scientific article

SlyD-dependent nickel delivery limits maturation of [NiFe]-hydrogenases in late-stationary phase Escherichia coli cells

scientific article published in April 2015

Structural diversity in twin-arginine signal peptide-binding proteins

scientific article

Structure and activity of ChiX, a peptidoglycan hydrolase required for chitinase secretion by Serratia marcescens .

scientific article published on 11 December 2017

Subunit composition and in vivo substrate-binding characteristics of Escherichia coli Tat protein complexes expressed at native levels.

scientific article published in December 2006

TatBC, TatB, and TatC form structurally autonomous units within the twin arginine protein transport system of Escherichia coli

scientific article published on 30 July 2007

TatD Is a Cytoplasmic Protein with DNase Activity

scientific article published in Journal of Biological Chemistry

The Escherichia coli Cell Division Protein and Model Tat Substrate SufI (FtsP) Localizes to the Septal Ring and Has a Multicopper Oxidase-Like Structure

scientific article

The Model [NiFe]-Hydrogenases of Escherichia coli

scientific article published on 23 March 2016

The Tat Protein Export Pathway

scientific article published on 01 September 2010

The Tat protein translocation pathway and its role in microbial physiology

scientific article published on January 1, 2003

The hows and whys of aerobic H2 metabolism ⬇️

scientific article published on February 25, 2012

The plant pathogen Pectobacterium atrosepticum contains a functional formate hydrogenlyase-2 complex

scientific article published on 10 September 2019

The structure of hydrogenase-2 from Escherichia coli: implications for H2-driven proton pumping

scientific article published on 19 March 2018

The twin-arginine transport system: moving folded proteins across membranes

scientific article

Towards an integrated system for bio-energy: hydrogen production by Escherichia coli and use of palladium-coated waste cells for electricity generation in a fuel cell.

scientific article

Transforming an oxygen-tolerant [NiFe] uptake hydrogenase into a proficient, reversible hydrogen producer

Water-gas shift reaction catalyzed by redox enzymes on conducting graphite platelets

scientific article published on October 2009

Zymographic differentiation of [NiFe]-hydrogenases 1, 2 and 3 of Escherichia coli K-12.

scientific article

List of works by Frank Sargent

A Novel Aerobic Mechanism for Reductive Palladium Biomineralization and Recovery byEscherichia coli

A genetic analysis of in vivo selenate reduction by Salmonella enterica serovar Typhimurium LT2 and Escherichia coli K12.

A genetic screen for suppressors of Escherichia coli Tat signal peptide mutations establishes a critical role for the second arginine within the twin-arginine motif

A holin and an endopeptidase are essential for chitinolytic protein secretion in Serratia marcescens

A holin/peptidoglycan hydrolase-dependent protein secretion system

A marriage of bacteriology with cell biology results in twin arginines

A novel protein transport system involved in the biogenesis of bacterial electron transfer chains

A regulatory domain controls the transport activity of a twin-arginine signal peptide

A subset of bacterial inner membrane proteins integrated by the twin-arginine translocase

A synthetic system for expression of components of a bacterial microcompartment

An essential component of a novel bacterial protein export system with homologues in plastids and mitochondria

Analysis of Tat targeting function and twin-arginine signal peptide activity in Escherichia coli

Analysis of hydrogenase 1 levels reveals an intimate link between carbon and hydrogen metabolism in Escherichia coli K-12.

Assembly of Tat-dependent [NiFe] hydrogenases: identification of precursor-binding accessory proteins

Assembly of membrane-bound respiratory complexes by the Tat protein-transport system

Bacterial formate hydrogenlyase complex

Behaviour of topological marker proteins targeted to the Tat protein transport pathway

Biorecovery of Precious Metals from Wastes and Conversion into Fuel Cell Catalyst for Electricity Production

Biosynthesis of Salmonella enterica [NiFe]-hydrogenase-5: probing the roles of system-specific accessory proteins

Biosynthesis of selenate reductase in Salmonella enterica: critical roles for the signal peptide and DmsD.

Biosynthesis of the respiratory formate dehydrogenases from Escherichia coli: characterization of the FdhE protein

Chaperones involved in assembly and export of N-oxide reductases

Characterisation of the membrane-extrinsic domain of the TatB component of the twin arginine protein translocase.

Characterization of a periplasmic nitrate reductase in complex with its biosynthetic chaperone

Characterization of a pre-export enzyme-chaperone complex on the twin-arginine transport pathway

Common principles in the biosynthesis of diverse enzymes.

Conserved Signal Peptide Recognition Systems across the Prokaryotic Domains

Constitutive expression of Escherichia coli tat genes indicates an important role for the twin-arginine translocase during aerobic and anaerobic growth

Constructing the wonders of the bacterial world: biosynthesis of complex enzymes

Controlling and co-ordinating chitinase secretion in a Serratia marcescens population

Coordinating assembly and export of complex bacterial proteins

Crystal structure of the O(2)-tolerant membrane-bound hydrogenase 1 from Escherichia coli in complex with its cognate cytochrome b

Crystal structure of the molybdenum cofactor biosynthesis protein MobA from Escherichia coli at near-atomic resolution

Design and characterisation of synthetic operons for biohydrogen technology

Dissecting the roles of Escherichia coli hydrogenases in biohydrogen production

Dissection and engineering of the Escherichia coli formate hydrogenlyase complex

Efficient Hydrogen-Dependent Carbon Dioxide Reduction by Escherichia coli.

Efficient electron transfer from hydrogen to benzyl viologen by the [NiFe]-hydrogenases of Escherichia coli is dependent on the coexpression of the iron-sulfur cluster-containing small subunit

Escherichia coli tat mutant strains are able to transport maltose in the absence of an active malE gene

Exploring the directionality of Escherichia coli formate hydrogenlyase: a membrane-bound enzyme capable of fixing carbon dioxide to organic acid

Export of complex cofactor-containing proteins by the bacterial Tat pathway

Features of a twin-arginine signal peptide required for recognition by a Tat proofreading chaperone

Functional complexity of the twin-arginine translocase TatC component revealed by site-directed mutagenesis

Harnessing Escherichia coli for Bio-Based Production of Formate under Pressurized H<sub>2</sub> and CO<sub>2</sub> Gases

How Escherichia coli is equipped to oxidize hydrogen under different redox conditions

How Escherichia coli is equipped to oxidize hydrogen under different redox conditions

How Salmonella oxidises H(2) under aerobic conditions

How oxygen reacts with oxygen-tolerant respiratory [NiFe]-hydrogenases

How the oxygen tolerance of a [NiFe]-hydrogenase depends on quaternary structure

How the structure of the large subunit controls function in an oxygen-tolerant [NiFe]-hydrogenase

Hydrogen production in the presence of oxygen by Escherichia coli K-12

Identification of a stable complex between a [NiFe]-hydrogenase catalytic subunit and its maturation protease.

Inactivation of the Escherichia coli K-12 twin-arginine translocation system promotes increased hydrogen production

Integration of an [FeFe]-hydrogenase into the anaerobic metabolism of Escherichia coli.

Intrinsic GTPase activity of a bacterial twin-arginine translocation proofreading chaperone induced by domain swapping

Involvement of hydrogenases in the formation of highly catalytic Pd(0) nanoparticles by bioreduction of Pd(II) using Escherichia coli mutant strains

Light traffic: photo-crosslinking a novel transport system

Look on the positive side! The orientation, identification and bioenergetics of 'Archaeal' membrane-bound nitrate reductases

Mechanism of hydrogen activation by [NiFe] hydrogenases

Membrane interactions and self-association of the TatA and TatB components of the twin-arginine translocation pathway

Oligomeric properties and signal peptide binding by Escherichia coli Tat protein transport complexes.

Overlapping functions of components of a bacterial Sec-independent protein export pathway

Overlapping transport and chaperone-binding functions within a bacterial twin-arginine signal peptide

Oxygen-tolerant [NiFe]-hydrogenases: the individual and collective importance of supernumerary cysteines at the proximal Fe-S cluster

Pathfinders and trailblazers: a prokaryotic targeting system for transport of folded proteins

Physiology and bioenergetics of [NiFe]-hydrogenase 2-catalyzed H2-consuming and H2-producing reactions in Escherichia coli

Principles of sustained enzymatic hydrogen oxidation in the presence of oxygen--the crucial influence of high potential Fe-S clusters in the electron relay of [NiFe]-hydrogenases.

Protein targeting by the bacterial twin-arginine translocation (Tat) pathway

Proteolytic processing of Escherichia coli twin-arginine signal peptides by LepB.

Purified components of the Escherichia coli Tat protein transport system form a double-layered ring structure

Regulation of the hydrogenase-4 operon of Escherichia coli by the sigma(54)-dependent transcriptional activators FhlA and HyfR.

Remnant signal peptides on non-exported enzymes: implications for the evolution of prokaryotic respiratory chains

Sec-independent protein translocation in Escherichia coli. A distinct and pivotal role for the TatB protein

Sequence analysis of bacterial redox enzyme maturation proteins (REMPs).

Signal peptide etiquette during assembly of a complex respiratory enzyme

Signal peptide-chaperone interactions on the twin-arginine protein transport pathway.

SlyD-dependent nickel delivery limits maturation of [NiFe]-hydrogenases in late-stationary phase Escherichia coli cells

Structural diversity in twin-arginine signal peptide-binding proteins

Structure and activity of ChiX, a peptidoglycan hydrolase required for chitinase secretion by Serratia marcescens .