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List of works by Barbara Ann Halkier

1,4-Dimethoxyglucobrassicin in Barbarea and 4-hydroxyglucobrassicin in Arabidopsis and Brassica.

scientific article published in March 2001

2-nitro-3-(p-hydroxyphenyl)propionate and aci-1-nitro-2-(p-hydroxyphenyl)ethane, two intermediates in the biosynthesis of the cyanogenic glucoside dhurrin in Sorghum bicolor (L.) Moench.

scientific article published on January 1991

A Western Blot Protocol for Detection of Proteins Heterologously Expressed in Xenopus laevis Oocytes

scientific article published in January 2016

A complex interplay of three R2R3 MYB transcription factors determines the profile of aliphatic glucosinolates in Arabidopsis

scientific article published on 26 March 2010

A method for expression cloning of transporter genes by screening yeast for uptake of radiolabelled substrate

scientific article published in May 2000

A novel 2-oxoacid-dependent dioxygenase involved in the formation of the goiterogenic 2-hydroxybut-3-enyl glucosinolate and generalist insect resistance in Arabidopsis,.

scientific article published on 22 October 2008

A systems biology approach identifies a R2R3 MYB gene subfamily with distinct and overlapping functions in regulation of aliphatic glucosinolates

scientific article

A unified nomenclature of NITRATE TRANSPORTER 1/PEPTIDE TRANSPORTER family members in plants.

scientific article

Advances in methods for identification and characterization of plant transporter function.

scientific article published on 3 May 2017

Advancing uracil-excision based cloning towards an ideal technique for cloning PCR fragments.

scientific article

Albugo-imposed changes to tryptophan-derived antimicrobial metabolite biosynthesis may contribute to suppression of non-host resistance to Phytophthora infestans in Arabidopsis thaliana

scientific article

Altering glucosinolate profiles modulates disease resistance in plants

scientific article published in June 2006

Analysis and Quantification of Glucosinolates

scientific article published on 10 June 2016

Arabidopsis cytochrome P450 monooxygenase 71A13 catalyzes the conversion of indole-3-acetaldoxime in camalexin synthesis

scientific article published on 15 June 2007

Arabidopsis glucosinolate storage cells transform into phloem fibres at late stages of development

scientific article published on 01 August 2019

Arabidopsis mutants in the C-S lyase of glucosinolate biosynthesis establish a critical role for indole-3-acetaldoxime in auxin homeostasis

scientific article (publication date: March 2004)

Arabidopsisgulliver1/superroot2-7identifies a metabolic basis for auxin and brassinosteroid synergy

scientific article published on 22 November 2014

Assigning gene function in biosynthetic pathways: camalexin and beyond

scientific article published in February 2013

Biochemical networks and epistasis shape the Arabidopsis thaliana metabolome

scientific article

Biology and biochemistry of glucosinolates

scientific article

Biosynthesis and metabolic engineering of glucosinolates.

scientific article

Biosynthesis of glucosinolates--gene discovery and beyond

scientific article published on 19 March 2010

Biosynthesis of the Cyanogenic Glucoside Dhurrin in Seedlings of Sorghum bicolor (L.) Moench and Partial Purification of the Enzyme System Involved

scientific article published in August 1989

Biotechnological approaches in glucosinolate production.

scientific article published in October 2018

CASCADE, a platform for controlled gene amplification for high, tunable and selection-free gene expression in yeast

scientific article published on 30 January 2017

CYP71B15 (PAD3) catalyzes the final step in camalexin biosynthesis

scientific article published in August 2006

CYP79B1 from Sinapis alba converts tryptophan to indole-3-acetaldoxime

scientific article

CYP79F1 and CYP79F2 have distinct functions in the biosynthesis of aliphatic glucosinolates in Arabidopsis

scientific article published in March 2003

CYP83A1 and CYP83B1, two nonredundant cytochrome P450 enzymes metabolizing oximes in the biosynthesis of glucosinolates in Arabidopsis

scientific article published in September 2003

CYP83b1 is the oxime-metabolizing enzyme in the glucosinolate pathway in Arabidopsis.

scientific article

Camalexin is synthesized from indole-3-acetaldoxime, a key branching point between primary and secondary metabolism in Arabidopsis

scientific article

Catalytic reactivities and structure/function relationships of cytochrome P450 enzymes

scientific article published in September 1996

Cellular and subcellular localization of flavin-monooxygenases involved in glucosinolate biosynthesis

scientific article published on 15 November 2010

Changing substrate specificity and iteration of amino acid chain elongation in glucosinolate biosynthesis through targeted mutagenesis of Arabidopsis methylthioalkylmalate synthase 1

scientific article published on 02 July 2019

Characterization of Glucosinolate Uptake by Leaf Protoplasts ofBrassica napus

scientific article published on 17 May 2000

Characterization of methylsulfinylalkyl glucosinolate specific polyclonal antibodies

scientific article published on 19 February 2016

Characterization of transgenic Arabidopsis thaliana with metabolically engineered high levels of p-hydroxybenzylglucosinolate

scientific article published in March 2001

Cloning and characterization of two cDNAs encoding sulfatases in the Roman snail, Helix pomatia

scientific article published in January 2000

Cloning and expression in Escherichia coli of the obtusifoliol 14 alpha-demethylase of Sorghum bicolor (L.) Moench, a cytochrome P450 orthologous to the sterol 14 alpha-demethylases (CYP51) from fungi and mammals

scientific article

Cloning of three A-type cytochromes P450, CYP71E1, CYP98, and CYP99 from Sorghum bicolor (L.) Moench by a PCR approach and identification by expression in Escherichia coli of CYP71E1 as a multifunctional cytochrome P450 in the biosynthesis of the cy

scientific article published in February 1998

Collection of Apoplastic Fluids from Arabidopsis thaliana Leaves

scientific article

Composition and content of glucosinolates in developing Arabidopsis thaliana

scientific article

Controlled indole-3-acetaldoxime production through ethanol-induced expression of CYP79B2.

scientific article published on 5 March 2009

Correction: Origin and evolution of transporter substrate specificity within the NPF family

scientific article published on 02 April 2019

Cytochrome P450 CYP79B2 from Arabidopsis catalyzes the conversion of tryptophan to indole-3-acetaldoxime, a precursor of indole glucosinolates and indole-3-acetic acid.

scientific article

Cytochrome p450 CYP79F1 from arabidopsis catalyzes the conversion of dihomomethionine and trihomomethionine to the corresponding aldoximes in the biosynthesis of aliphatic glucosinolates

scientific article

Cytochromes P450 in the biosynthesis of glucosinolates and indole alkaloids

scientific article published on 28 October 2006

Cytosolic γ-glutamyl peptidases process glutathione conjugates in the biosynthesis of glucosinolates and camalexin in Arabidopsis

scientific article published on 28 June 2011

De novo genetic engineering of the camalexin biosynthetic pathway

scientific article published on 3 July 2013

De novo production of benzyl glucosinolate in Escherichia coli

scientific article published on 01 March 2019

Determination of the absolute configuration of the glucosinolate methyl sulfoxide group reveals a stereospecific biosynthesis of the side chain

scientific article published on 20 October 2008

Differential Roles of Glucosinolates and Camalexin at Different Stages of Agrobacterium-Mediated Transformation

scientific article published on 2 March 2018

Differential effects of indole and aliphatic glucosinolates on lepidopteran herbivores.

scientific article published on 9 July 2010

Dynamic Modeling of Indole Glucosinolate Hydrolysis and Its Impact on Auxin Signaling

scientific article published in 2018

Elucidating the role of transport processes in leaf glucosinolate distribution.

scientific article published on 10 September 2014

Engineering of benzylglucosinolate in tobacco provides proof-of-concept for dead-end trap crops genetically modified to attract Plutella xylostella (diamondback moth).

scientific article published on 19 January 2012

Engineering of glucosinolate biosynthesis: candidate gene identification and validation

scientific article published in January 2012

Engineering of methionine chain elongation part of glucoraphanin pathway in E. coli

scientific article published on 24 September 2015

Expression of the Arabidopsis high-affinity hexose transporter STP13 correlates with programmed cell death

scientific article published in April 2006

Feeding on Leaves of the Glucosinolate Transporter Mutant gtr1gtr2 Reduces Fitness of Myzus persicae

scientific article

Functional Expression and Characterization of Plant ABC Transporters in Xenopus laevis Oocytes for Transport Engineering Purposes

scientific article

Functional Expression and Characterization of the Myrosinase MYR1 from Brassica napus in Saccharomyces cerevisiae

scientific article published in December 1999

Functional analysis of the tandem-duplicated P450 genes SPS/BUS/CYP79F1 and CYP79F2 in glucosinolate biosynthesis and plant development by Ds transposition-generated double mutants

scientific article published on 11 June 2004

GTR-Mediated Radial Import Directs Accumulation of Defensive Glucosinolates to Sulfur-Rich Cells in the Phloem Cap of Arabidopsis Inflorescence Stem

scientific article published on 29 June 2019

Genes involved in the evolution of herbivory by a leaf-mining, Drosophilid fly

scientific article (publication date: 2012)

Glucosinolate engineering identifies a gamma-glutamyl peptidase.

scientific article published on 31 May 2009

Glucosinolate research in the Arabidopsis era.

scientific article

Grafting Arabidopsis

scientific article published in 2014

Herbivore feeding preference corroborates optimal defense theory for specialized metabolites within plants

scientific article published on 01 November 2021

How does a plant orchestrate defense in time and space? Using glucosinolates in Arabidopsis as case study.

scientific article published on 30 May 2017

How to discover a metabolic pathway? An update on gene identification in aliphatic glucosinolate biosynthesis, regulation and transport

scientific article published on May 2014

How to prove the existence of metabolons?

scientific article

Identification of Iridoid Glucoside Transporters in Catharanthus roseus.

scientific article published in September 2017

Identification of a flavin-monooxygenase as the S-oxygenating enzyme in aliphatic glucosinolate biosynthesis in Arabidopsis

scientific article published on 25 April 2007

Identification of genes involved in shea butter biosynthesis from Vitellaria paradoxa fruits through transcriptomics and functional heterologous expression

article

Identifying the molecular basis of QTLs: eQTLs add a new dimension

scientific article published on 11 February 2008

Improving analytical methods for protein-protein interaction through implementation of chemically inducible dimerization

scientific article published on 10 June 2016

In vivo synthesis and purification of radioactivep-hydroxybenzylglucosinolate inSinapis alba L

scientific article published in May 2000

Indole-3-acetaldoxime-derived compounds restrict root colonization in the beneficial interaction between Arabidopsis roots and the endophyte Piriformospora indica.

scientific article

Integration of biosynthesis and long-distance transport establish organ-specific glucosinolate profiles in vegetative Arabidopsis

scientific article published on 30 August 2013

Involvement of Cytochrome P-450 in the Biosynthesis of Dhurrin in Sorghum bicolor (L.) Moench

scientific article published in May 1991

Involvement of cytochrome P450 in oxime production in glucosinolate biosynthesis as demonstrated by an in vitro microsomal enzyme system isolated from jasmonic acid-induced seedlings of Sinapis alba L

scientific article

Isolation and reconstitution of cytochrome P450ox and in vitro reconstitution of the entire biosynthetic pathway of the cyanogenic glucoside dhurrin from sorghum

scientific article (publication date: December 1997)

Isolation of the heme-thiolate enzyme cytochrome P-450TYR, which catalyzes the committed step in the biosynthesis of the cyanogenic glucoside dhurrin in Sorghum bicolor (L.) Moench.

scientific article published on October 1994

Linking metabolic QTLs with network and cis-eQTLs controlling biosynthetic pathways

scientific article

Localization of the glucosinolate biosynthetic enzymes reveals distinct spatial patterns for the biosynthesis of indole and aliphatic glucosinolates

scientific article

Long-distance phloem transport of glucosinolates in Arabidopsis

scientific article

Metabolic engineering in Nicotiana benthamiana reveals key enzyme functions in Arabidopsis indole glucosinolate modification.

scientific article published on 11 February 2011

Metabolic engineering of p-hydroxybenzylglucosinolate in Arabidopsis by expression of the cyanogenic CYP79A1 from Sorghum bicolor

scientific article published in December 1999

Metabolic engineering of valine- and isoleucine-derived glucosinolates in Arabidopsis expressing CYP79D2 from Cassava

scientific article published in February 2003

Microbial production of indolylglucosinolate through engineering of a multi-gene pathway in a versatile yeast expression platform

scientific article published in March 2012

Mineralization of benzyl glucosinolate and its hydrolysis product the biofumigant benzyl isothiocyanate in soil

scientific article published in January 2008

Modulation of CYP79 genes and glucosinolate profiles in Arabidopsis by defense signaling pathways

scientific article published in January 2003

Modulation of sulfur metabolism enables efficient glucosinolate engineering

scientific article

NRT/PTR transporters are essential for translocation of glucosinolate defence compounds to seeds

scientific article published in August 2012

Natural variation in cross-talk between glucosinolates and onset of flowering in Arabidopsis

scientific article

Non-volatile intact indole glucosinolates are host recognition cues for ovipositing Plutella xylostella

scientific article published on December 2009

Optimization of Engineered Production of the Glucoraphanin Precursor Dihomomethionine in Nicotiana benthamiana

scientific article published on 16 February 2016

Origin and evolution of transporter substrate specificity within the NPF family

scientific article published on 03 March 2017

Piecing together the transport pathway of aliphatic glucosinolates

scientific article published on 13 November 2008

Production of the cancer-preventive glucoraphanin in tobacco

scientific article published on 9 May 2010

Purification and characterization of recombinant cytochrome P450TYR expressed at high levels in Escherichia coli.

scientific article

Rapid stimulation of a soybean protein-serine kinase that phosphorylates a novel bZIP DNA-binding protein, G/HBF-1, during the induction of early transcription-dependent defenses.

scientific article

Reduction of antinutritional glucosinolates in Brassica oilseeds by mutation of genes encoding transporters.

scientific article published on 13 March 2017

Regulatory networks of glucosinolates shape Arabidopsis thaliana fitness

scientific article published on 11 March 2010

Responses of the flea beetles Phyllotreta nemorum and P. cruciferae to metabolically engineered Arabidopsis thaliana with an altered glucosinolate profile

scientific article published in April 2001

Rhizosecretion of stele-synthesized glucosinolates and their catabolites requires GTR-mediated import in Arabidopsis.

scientific article

Screening for plant transporter function by expressing a normalized Arabidopsis full-length cDNA library in Xenopus oocytes

scientific article

Subclade of flavin-monooxygenases involved in aliphatic glucosinolate biosynthesis

scientific article

Substrate specificity of the cytochrome P450 enzymes CYP79A1 and CYP71E1 involved in the biosynthesis of the cyanogenic glucoside dhurrin in Sorghum bicolor (L.) Moench.

scientific article

Synthesis of 1-nitro-2-(p-hydroxyphenyl)[2-3H1]ethane

scientific article published in January 1991

The Arabidopsis NPF3 protein is a GA transporter

scientific article published on 3 May 2016

The Glucosinolate Biosynthetic Gene AOP2 Mediates Feed-back Regulation of Jasmonic Acid Signaling in Arabidopsis

scientific article published on 7 March 2015

The biosynthesis of glucosinolates

scientific article published in November 1997

The emerging field of transport engineering of plant specialized metabolites

scientific article published on 04 October 2012

The influence of metabolically engineered glucosinolates profiles in Arabidopsis thaliana on Plutella xylostella preference and performance

scientific article

The presence of CYP79 homologues in glucosinolate-producing plants shows evolutionary conservation of the enzymes in the conversion of amino acid to aldoxime in the biosynthesis of cyanogenic glucosides and glucosinolates

scientific article published in November 1998

Towards engineering glucosinolates into non-cruciferous plants

scientific article

Transgenic tobacco and Arabidopsis plants expressing the two multifunctional sorghum cytochrome P450 enzymes, CYP79A1 and CYP71E1, are cyanogenic and accumulate metabolites derived from intermediates in Dhurrin biosynthesis

scientific article

Transport of defense compounds from source to sink: lessons learned from glucosinolates

scientific article published on 12 May 2015

USER Cloning and USER Fusion: The Ideal Cloning Techniques for Small and Big Laboratories

scientific article published in 2010

USER fusion: a rapid and efficient method for simultaneous fusion and cloning of multiple PCR products

scientific article published on 27 March 2007

USER-Derived Cloning Methods and Their Primer Design

scientific article published on 10 December 2013

Unravelling Protein-Protein Interaction Networks Linked to Aliphatic and Indole Glucosinolate Biosynthetic Pathways in Arabidopsis.

scientific article published on 29 November 2017

Uptake Assays in Xenopus laevis Oocytes Using Liquid Chromatography-mass Spectrometry to Detect Transport Activity

scientific article published in 2017

[30] Isolation of plant and recombinant CYP79

scientific article published in 1996