List of works - Thomas Happe

<scp><i>Chlamydomonas reinhardtii</i></scp> mutants deficient for Old Yellow Enzyme 3 exhibit increased photooxidative stress

scientific article published in 2023

A novel screening protocol for the isolation of hydrogen producing Chlamydomonas reinhardtii strains

scientific article

A novel type of iron hydrogenase in the green alga Scenedesmus obliquus is linked to the photosynthetic electron transport chain.

scientific article

A novel, anaerobically induced ferredoxin in Chlamydomonas reinhardtii.

scientific article published on 26 December 2008

A pyruvate formate lyase-deficient Chlamydomonas reinhardtii strain provides evidence for a link between fermentation and hydrogen production in green algae.

scientific article

A redox hydrogel protects the O2 -sensitive [FeFe]-hydrogenase from Chlamydomonas reinhardtii from oxidative damage

scientific article published on 12 June 2015

A structural view of synthetic cofactor integration into [FeFe]-hydrogenases.

scientific article published on 26 October 2015

Accumulating the hydride state in the catalytic cycle of [FeFe]-hydrogenases

scientific article published on 19 July 2017

Alternative photosynthetic electron transport pathways during anaerobiosis in the green alga Chlamydomonas reinhardtii.

scientific article published on March 2011

Anaerobic expression of the ferredoxin-encoding FDX5 gene of Chlamydomonas reinhardtii is regulated by the Crr1 transcription factor

scientific article published on 10 September 2010

Analytical approaches to photobiological hydrogen production in unicellular green algae

scientific article

Association of Ferredoxin:NADP+ oxidoreductase with the photosynthetic apparatus modulates electron transfer in Chlamydomonas reinhardtii.

scientific article published on 7 June 2017

Autotrophic and mixotrophic hydrogen photoproduction in sulfur-deprived chlamydomonas cells

scientific article (publication date: October 2005)

Biochemical and morphological characterization of sulfur-deprived and H2-producing Chlamydomonas reinhardtii (green alga).

scientific article published in February 2002

Biomimetic assembly and activation of [FeFe]-hydrogenases

scientific article published on 26 June 2013

Chalcogenide substitution in the [2Fe] cluster of [FeFe]-hydrogenases conserves high enzymatic activity.

scientific article published on 27 November 2017

Compartmentalisation of [FeFe]-hydrogenase maturation in Chlamydomonas reinhardtii.

scientific article published on 13 March 2017

Control of hydrogen photoproduction by the proton gradient generated by cyclic electron flow in Chlamydomonas reinhardtii

scientific article

Copper response regulator1-dependent and -independent responses of the Chlamydomonas reinhardtii transcriptome to dark anoxia

scientific article published on 6 September 2013

Crystallographic and spectroscopic assignment of the proton transfer pathway in [FeFe]-hydrogenases

scientific article published in Nature Communications

Cyanobacterial H2 production ? a comparative analysis

scientific article published on 15 October 2003

Differential Protonation at the Catalytic Six-Iron Cofactor of [FeFe]-Hydrogenases Revealed by 57Fe Nuclear Resonance X-ray Scattering and Quantum Mechanics/Molecular Mechanics Analyses

scientific article published on 25 February 2019

Electrochemical Investigations of the Mechanism of Assembly of the Active-Site H-Cluster of [FeFe]-Hydrogenases.

scientific article published on 24 October 2016

Expression of two [Fe]-hydrogenases in Chlamydomonas reinhardtii under anaerobic conditions

scientific article

Flavodiiron-Mediated O2 Photoreduction Links H2 Production with CO2 Fixation during the Anaerobic Induction of Photosynthesis

scientific article published on 05 July 2018

Formaldehyde--a rapid and reversible inhibitor of hydrogen production by [FeFe]-hydrogenases

scientific article published on 04 January 2011

Frequency and potential dependence of reversible electrocatalytic hydrogen interconversion by [FeFe]-hydrogenases.

scientific article published on 27 March 2017

Genomics of green algal hydrogen research

scientific article published in January 2004

His-Ligation to the [4Fe-4S] Subcluster Tunes the Catalytic Bias of [FeFe] Hydrogenase

scientific article published on 26 December 2018

Homologous and heterologous overexpression in Clostridium acetobutylicum and characterization of purified clostridial and algal Fe-only hydrogenases with high specific activities

scientific article published on May 2005

How Formaldehyde Inhibits Hydrogen Evolution by [FeFe]-Hydrogenases: Determination by ¹³C ENDOR of Direct Fe-C Coordination and Order of Electron and Proton Transfers

scientific article

How [FeFe]-Hydrogenase Facilitates Bidirectional Proton Transfer

scientific article published on 15 October 2019

How algae produce hydrogen--news from the photosynthetic hydrogenase.

scientific article published on 22 October 2009

How oxygen attacks [FeFe] hydrogenases from photosynthetic organisms

scientific article

HoxE--a subunit specific for the pentameric bidirectional hydrogenase complex (HoxEFUYH) of cyanobacteria.

scientific article published in April 2002

Hydride binding to the active site of [FeFe]-hydrogenase.

scientific article published on 4 November 2014

Hydrogen production by Chlamydomonas reinhardtii: an elaborate interplay of electron sources and sinks.

scientific article published on 21 September 2007

Hydrogen production. Green algae as a source of energy

scientific article

Hydrogenases in green algae: do they save the algae's life and solve our energy problems?

scientific article published on 01 June 2002

Hypoxic survival requires a 2-on-2 hemoglobin in a process involving nitric oxide

scientific article published on 10 June 2013

Identification and characterization of the "super-reduced" state of the H-cluster in [FeFe] hydrogenase: a new building block for the catalytic cycle?

scientific article published on 26 October 2012

Immobilization of the [FeFe]-hydrogenase CrHydA1 on a gold electrode: design of a catalytic surface for the production of molecular hydrogen.

scientific article published on 6 February 2009

Importance of the protein framework for catalytic activity of [FeFe]-hydrogenases.

scientific article published on 22 November 2011

Induction, localization and metal content of hydrogenase in the green alga Chlamydomonas reinhardtii

scientific article published on 01 June 1994

Influence of the [4Fe-4S] cluster coordinating cysteines on active site maturation and catalytic properties of C. reinhardtii [FeFe]-hydrogenase.

scientific article published on 9 October 2017

Inhibition of [FeFe]-Hydrogenases by Formaldehyde and Wider Mechanistic Implications for Biohydrogen Activation

scientific article published on 18 April 2012

Iron hydrogenases--ancient enzymes in modern eukaryotes.

scientific article

Iron-sulphur cluster biogenesis via the SUF pathway

scientific article published on 01 August 2018

Isolation and first EPR characterization of the [FeFe]-hydrogenases from green algae

scientific article published on 21 February 2008

Isolation and molecular characterization of the [Fe]-hydrogenase from the unicellular green alga Chlorella fusca

scientific article published in July 2002

Isolation, characterization and N-terminal amino acid sequence of hydrogenase from the green alga Chlamydomonas reinhardtii.

scientific article published in June 1993

Light driven hydrogen production in protein based semi-artificial systems.

scientific article published on 19 May 2011

Loss of Specific Active-Site Iron Atoms in Oxygen-Exposed [FeFe]-Hydrogenase Determined by Detailed X-ray Structure Analyses

scientific article published on 25 October 2019

Lyophilization protects [FeFe]-hydrogenases against O2-induced H-cluster degradation.

scientific article

Metalloprotein mimics - old tools in a new light

scientific article published on 11 March 2014

Molecular basis of [FeFe]-hydrogenase function: an insight into the complex interplay between protein and catalytic cofactor.

scientific article

Molecular cloning and characterization of hetR genes from filamentous cyanobacteria

scientific article published in August 2002

New redox states observed in [FeFe] hydrogenases reveal redox coupling within the H-cluster

scientific article published on 29 July 2014

Nitrogen deprivation results in photosynthetic hydrogen production in Chlamydomonas reinhardtii

scientific article published on 22 October 2011

O2 reactions at the six-iron active site (H-cluster) in [FeFe]-hydrogenase

scientific article published on 19 September 2011

Preventing the coffee-ring effect and aggregate sedimentation by gelation of monodisperse materials

article by Huaiguang Li et al published 21 October 2018 in Chemical Science

Proton-Coupled Reduction of the Catalytic [4Fe-4S] Cluster in [FeFe]-Hydrogenases

scientific article published on 26 October 2017

Pyruvate:ferredoxin oxidoreductase is coupled to light-independent hydrogen production in Chlamydomonas reinhardtii.

scientific article

Quantum yield measurements of light-induced H₂ generation in a photosystem I-[FeFe]-H₂ase nanoconstruct

scientific article published on 20 December 2014

Shedding Light on Proton and Electron Dynamics in [FeFe] Hydrogenases

scientific article published on 16 March 2020

Solar hydrogen-producing bionanodevice outperforms natural photosynthesis.

scientific article published on 12 December 2011

Solvent dynamics play a decisive role in the complex formation of biologically relevant redox proteins

scientific article published on 01 April 2020

Spectroelectrochemical characterization of the active site of the [FeFe] hydrogenase HydA1 from Chlamydomonas reinhardtii

scientific article published in August 2009

Spontaneous activation of [FeFe]-hydrogenases by an inorganic [2Fe] active site mimic

scientific article published on 11 August 2013

Sunlight-Dependent Hydrogen Production by Photosensitizer/Hydrogenase Systems

scientific article published on 15 December 2016

Sustained H2 production in a Chlamydomonas reinhardtii D1 protein mutant

article

Tailor-Made Modification of a Gold Surface for the Chemical Binding of a High-Activity [FeFe] Hydrogenase

The [FeFe]-hydrogenase maturase HydF from Clostridium acetobutylicum contains a CO and CN- ligated iron cofactor

scientific article published on 14 December 2009

The [FeFe]-hydrogenase maturation protein HydF contains a H-cluster like [4Fe4S]-2Fe site

scientific article published on 03 December 2010

The exceptional photofermentative hydrogen metabolism of the green alga Chlamydomonas reinhardtii.

scientific article published in February 2005

The final steps of [FeFe]-hydrogenase maturation

scientific article published on 23 July 2019

The roles of long-range proton-coupled electron transfer in the directionality and efficiency of [FeFe]-hydrogenases

scientific article published on 13 August 2020

The structurally unique photosynthetic Chlorella variabilis NC64A hydrogenase does not interact with plant-type ferredoxins

scientific article published on 21 June 2017

The structure of the active site H-cluster of [FeFe] hydrogenase from the green alga Chlamydomonas reinhardtii studied by X-ray absorption spectroscopy.

scientific article

Vibrational spectroscopy reveals the initial steps of biological hydrogen evolution

scientific article published on 11 July 2016

Wiring an [FeFe]-hydrogenase with photosystem I for light-induced hydrogen production.

scientific article

[FeFe]-Hydrogenase with Chalcogenide Substitutions at the H-Cluster Maintains Full H2 Evolution Activity.

scientific article published on 23 May 2016

[FeFe]-hydrogenases from green algae

scientific article published on 24 November 2018

List of works by Thomas Happe

<scp><i>Chlamydomonas reinhardtii</i></scp> mutants deficient for Old Yellow Enzyme 3 exhibit increased photooxidative stress

A novel screening protocol for the isolation of hydrogen producing Chlamydomonas reinhardtii strains

A novel type of iron hydrogenase in the green alga Scenedesmus obliquus is linked to the photosynthetic electron transport chain.

A novel, anaerobically induced ferredoxin in Chlamydomonas reinhardtii.

A pyruvate formate lyase-deficient Chlamydomonas reinhardtii strain provides evidence for a link between fermentation and hydrogen production in green algae.

A redox hydrogel protects the O2 -sensitive [FeFe]-hydrogenase from Chlamydomonas reinhardtii from oxidative damage

A structural view of synthetic cofactor integration into [FeFe]-hydrogenases.

Accumulating the hydride state in the catalytic cycle of [FeFe]-hydrogenases

Alternative photosynthetic electron transport pathways during anaerobiosis in the green alga Chlamydomonas reinhardtii.

Anaerobic expression of the ferredoxin-encoding FDX5 gene of Chlamydomonas reinhardtii is regulated by the Crr1 transcription factor

Analytical approaches to photobiological hydrogen production in unicellular green algae

Association of Ferredoxin:NADP+ oxidoreductase with the photosynthetic apparatus modulates electron transfer in Chlamydomonas reinhardtii.

Autotrophic and mixotrophic hydrogen photoproduction in sulfur-deprived chlamydomonas cells

Biochemical and morphological characterization of sulfur-deprived and H2-producing Chlamydomonas reinhardtii (green alga).

Biomimetic assembly and activation of [FeFe]-hydrogenases

Chalcogenide substitution in the [2Fe] cluster of [FeFe]-hydrogenases conserves high enzymatic activity.

Compartmentalisation of [FeFe]-hydrogenase maturation in Chlamydomonas reinhardtii.

Control of hydrogen photoproduction by the proton gradient generated by cyclic electron flow in Chlamydomonas reinhardtii

Copper response regulator1-dependent and -independent responses of the Chlamydomonas reinhardtii transcriptome to dark anoxia

Crystallographic and spectroscopic assignment of the proton transfer pathway in [FeFe]-hydrogenases

Cyanobacterial H2 production ? a comparative analysis

Differential Protonation at the Catalytic Six-Iron Cofactor of [FeFe]-Hydrogenases Revealed by 57Fe Nuclear Resonance X-ray Scattering and Quantum Mechanics/Molecular Mechanics Analyses

Electrochemical Investigations of the Mechanism of Assembly of the Active-Site H-Cluster of [FeFe]-Hydrogenases.

Expression of two [Fe]-hydrogenases in Chlamydomonas reinhardtii under anaerobic conditions

Flavodiiron-Mediated O2 Photoreduction Links H2 Production with CO2 Fixation during the Anaerobic Induction of Photosynthesis

Formaldehyde--a rapid and reversible inhibitor of hydrogen production by [FeFe]-hydrogenases

Frequency and potential dependence of reversible electrocatalytic hydrogen interconversion by [FeFe]-hydrogenases.

Genomics of green algal hydrogen research

His-Ligation to the [4Fe-4S] Subcluster Tunes the Catalytic Bias of [FeFe] Hydrogenase

Homologous and heterologous overexpression in Clostridium acetobutylicum and characterization of purified clostridial and algal Fe-only hydrogenases with high specific activities

How Formaldehyde Inhibits Hydrogen Evolution by [FeFe]-Hydrogenases: Determination by ¹³C ENDOR of Direct Fe-C Coordination and Order of Electron and Proton Transfers

How [FeFe]-Hydrogenase Facilitates Bidirectional Proton Transfer

How algae produce hydrogen--news from the photosynthetic hydrogenase.

How oxygen attacks [FeFe] hydrogenases from photosynthetic organisms

HoxE--a subunit specific for the pentameric bidirectional hydrogenase complex (HoxEFUYH) of cyanobacteria.

Hydride binding to the active site of [FeFe]-hydrogenase.

Hydrogen production by Chlamydomonas reinhardtii: an elaborate interplay of electron sources and sinks.

Hydrogen production. Green algae as a source of energy

Hydrogenases in green algae: do they save the algae's life and solve our energy problems?

Hypoxic survival requires a 2-on-2 hemoglobin in a process involving nitric oxide

Identification and characterization of the "super-reduced" state of the H-cluster in [FeFe] hydrogenase: a new building block for the catalytic cycle?

Immobilization of the [FeFe]-hydrogenase CrHydA1 on a gold electrode: design of a catalytic surface for the production of molecular hydrogen.

Importance of the protein framework for catalytic activity of [FeFe]-hydrogenases.

Induction, localization and metal content of hydrogenase in the green alga Chlamydomonas reinhardtii

Influence of the [4Fe-4S] cluster coordinating cysteines on active site maturation and catalytic properties of C. reinhardtii [FeFe]-hydrogenase.

Inhibition of [FeFe]-Hydrogenases by Formaldehyde and Wider Mechanistic Implications for Biohydrogen Activation

Iron hydrogenases--ancient enzymes in modern eukaryotes.

Iron-sulphur cluster biogenesis via the SUF pathway

Isolation and first EPR characterization of the [FeFe]-hydrogenases from green algae

Isolation and molecular characterization of the [Fe]-hydrogenase from the unicellular green alga Chlorella fusca

Isolation, characterization and N-terminal amino acid sequence of hydrogenase from the green alga Chlamydomonas reinhardtii.

Light driven hydrogen production in protein based semi-artificial systems.

Loss of Specific Active-Site Iron Atoms in Oxygen-Exposed [FeFe]-Hydrogenase Determined by Detailed X-ray Structure Analyses

Lyophilization protects [FeFe]-hydrogenases against O2-induced H-cluster degradation.

Metalloprotein mimics - old tools in a new light

Molecular basis of [FeFe]-hydrogenase function: an insight into the complex interplay between protein and catalytic cofactor.

Molecular cloning and characterization of hetR genes from filamentous cyanobacteria

New redox states observed in [FeFe] hydrogenases reveal redox coupling within the H-cluster

Nitrogen deprivation results in photosynthetic hydrogen production in Chlamydomonas reinhardtii

O2 reactions at the six-iron active site (H-cluster) in [FeFe]-hydrogenase

Preventing the coffee-ring effect and aggregate sedimentation by gelation of monodisperse materials

Proton-Coupled Reduction of the Catalytic [4Fe-4S] Cluster in [FeFe]-Hydrogenases

Pyruvate:ferredoxin oxidoreductase is coupled to light-independent hydrogen production in Chlamydomonas reinhardtii.

Quantum yield measurements of light-induced H₂ generation in a photosystem I-[FeFe]-H₂ase nanoconstruct

Shedding Light on Proton and Electron Dynamics in [FeFe] Hydrogenases

Solar hydrogen-producing bionanodevice outperforms natural photosynthesis.

Solvent dynamics play a decisive role in the complex formation of biologically relevant redox proteins

Spectroelectrochemical characterization of the active site of the [FeFe] hydrogenase HydA1 from Chlamydomonas reinhardtii

Spontaneous activation of [FeFe]-hydrogenases by an inorganic [2Fe] active site mimic

Sunlight-Dependent Hydrogen Production by Photosensitizer/Hydrogenase Systems

Sustained H2 production in a Chlamydomonas reinhardtii D1 protein mutant

Tailor-Made Modification of a Gold Surface for the Chemical Binding of a High-Activity [FeFe] Hydrogenase

The [FeFe]-hydrogenase maturase HydF from Clostridium acetobutylicum contains a CO and CN- ligated iron cofactor

The [FeFe]-hydrogenase maturation protein HydF contains a H-cluster like [4Fe4S]-2Fe site

The exceptional photofermentative hydrogen metabolism of the green alga Chlamydomonas reinhardtii.

The final steps of [FeFe]-hydrogenase maturation

The roles of long-range proton-coupled electron transfer in the directionality and efficiency of [FeFe]-hydrogenases

The structurally unique photosynthetic Chlorella variabilis NC64A hydrogenase does not interact with plant-type ferredoxins

The structure of the active site H-cluster of [FeFe] hydrogenase from the green alga Chlamydomonas reinhardtii studied by X-ray absorption spectroscopy.