List of works - Pablo I Nikel

A Metabolic Widget Adjusts the Phosphoenolpyruvate-Dependent Fructose Influx in Pseudomonas putida.

scientific article

A New Player in the Biorefineries Field: Phasin PhaP Enhances Tolerance to Solvents and Boosts Ethanol and 1,3-Propanediol Synthesis in Escherichia coli.

scientific article published on 5 May 2017

A Post-translational Metabolic Switch Enables Complete Decoupling of Bacterial Growth from Biopolymer Production in Engineered Escherichia coli

article by Gonzalo Durante-Rodríguez et al published 22 October 2018 in ACS Synthetic Biology

A fluoride-responsive genetic circuit enables in vivo biofluorination in engineered Pseudomonas putida

scientific article published on 07 October 2020

Accelerated genome engineering of Pseudomonas putida by I-SceI-mediated recombination and CRISPR-Cas9 counterselection

scientific article published on 12 March 2019

Accumulation of inorganic polyphosphate enables stress endurance and catalytic vigour in Pseudomonas putida KT2440.

scientific article published on 20 May 2013

An expanded CRISPRi toolbox for tunable control of gene expression in Pseudomonas putida

scientific article published on 11 February 2020

Anr, the anaerobic global regulator, modulates the redox state and oxidative stress resistance in Pseudomonas extremaustralis

scientific article published on 6 December 2012

ArcA redox mutants as a source of reduced bioproducts.

scientific article

ArsH protects Pseudomonas putida from oxidative damage caused by exposure to arsenic

scientific article published on 23 March 2020

Biochemistry, genetics and biotechnology of glycerol utilization in Pseudomonas species

scientific article published on 18 March 2019

Biotechnological domestication of pseudomonads using synthetic biology.

scientific article published on May 2014

Biotransformation of 2,4-dinitrotoluene in a phototrophic co-culture of engineered Synechococcus elongatus and Pseudomonas putida

scientific article published on 16 February 2020

Breaking the state-of-the-art in the chemical industry with new-to-Nature products via synthetic microbiology

scientific article published on 31 January 2019

Chasing bacterial chassis for metabolic engineering: a perspective review from classical to non-traditional microorganisms

scientific article published on 21 June 2018

Combinatorial pathway balancing provides biosynthetic access to 2-fluoro-cis,cis-muconate in engineered Pseudomonas putida

scientific article published on 01 November 2021

Data on the standardization of a cyclohexanone-responsive expression system for Gram-negative bacteria

scientific article

Dual Effect: High NADH Levels Contribute to Efflux-Mediated Antibiotic Resistance but Drive Lethality Mediated by Reactive Oxygen Species

scientific article published in 2022

Effects of granule-associated protein PhaP on glycerol-dependent growth and polymer production in poly(3-hydroxybutyrate)-producing Escherichia coli

scientific article published on 26 October 2007

Elimination of D-lactate synthesis increases poly(3-hydroxybutyrate) and ethanol synthesis from glycerol and affects cofactor distribution in recombinant Escherichia coli

scientific article published on 24 September 2010

Endogenous stress caused by faulty oxidation reactions fosters evolution of 2,4-dinitrotoluene-degrading bacteria

scientific article (publication date: August 2013)

Engineering an anaerobic metabolic regime in Pseudomonas putida KT2440 for the anoxic biodegradation of 1,3-dichloroprop-1-ene ⬇️

scientific article published on November 10, 2012

Escherichia coli arcA mutants: metabolic profile characterization of microaerobic cultures using glycerol as a carbon source

scientific article

Escherichia coli redox mutants as microbial cell factories for the synthesis of reduced biochemicals.

scientific article

Ethanol synthesis from glycerol by Escherichia coli redox mutants expressing adhE from Leuconostoc mesenteroides.

scientific article published on 11 January 2010

Evolutionary Approaches for Engineering Industrially Relevant Phenotypes in Bacterial Cell Factories

scientific article published on 12 June 2019

Evolving metabolism of 2,4-dinitrotoluene triggers SOS-independent diversification of host cells

scientific article published on 03 December 2018

Exacerbation of substrate toxicity by IPTG in Escherichia coli BL21(DE3) carrying a synthetic metabolic pathway

scientific article published on 21 December 2015

Exploring the synthetic biology potential of bacteriophages for engineering non-model bacteria

scientific article published on 20 October 2020

Genetic programming of catalytic Pseudomonas putida biofilms for boosting biodegradation of haloalkanes

scientific article published on 24 November 2015

Genome reduction boosts heterologous gene expression in Pseudomonas putida

scientific article

Growth-coupled selection of synthetic modules to accelerate cell factory development

scientific article published in 2021

High-throughput dilution-based growth method enables time-resolved exo-metabolomics of Pseudomonas putida and Pseudomonas aeruginosa

scientific article published on 29 July 2021

Implantation of unmarked regulatory and metabolic modules in Gram-negative bacteria with specialised mini-transposon delivery vectors ⬇️

article by Pablo I Nikel & Victor de Lorenzo published January 2013 in Journal of Biotechnology

Manipulation of the anoxic metabolism in Escherichia coli by ArcB deletion variants in the ArcBA two-component system

scientific article published on 12 October 2012

Metabolic and regulatory rearrangements underlying glycerol metabolism in Pseudomonas putida KT2440.

scientific article published on 22 August 2013

Metabolic selective pressure stabilizes plasmids carrying biosynthetic genes for reduced biochemicals in Escherichia coli redox mutants.

scientific article published on 30 July 2010

Modelling the freezing response of baker's yeast prestressed cells: a statistical approach.

scientific article published on 9 October 2007

Modular (de)construction of complex bacterial phenotypes by CRISPR/nCas9-assisted, multiplex cytidine base-editing

scientific article published on 31 May 2022

New recombinant Escherichia coli strain tailored for the production of poly(3-hydroxybutyrate) from agroindustrial by-products.

scientific article published on June 2006

New transposon tools tailored for metabolic engineering of gram-negative microbial cell factories.

scientific article

Non-invasive, ratiometric determination of intracellular pH in Pseudomonas species using a novel genetically encoded indicator

scientific article published on 04 June 2019

Physical decoupling of XylS/Pm regulatory elements and conditional proteolysis enable precise control of gene expression in Pseudomonas putida

scientific article published on 12 March 2019

Poly(3-hydroxybutyrate) synthesis by recombinant Escherichia coli arcA mutants in microaerobiosis.

scientific article published on April 2006

Poly(3-hydroxybutyrate) synthesis from glycerol by a recombinant Escherichia coli arcA mutant in fed-batch microaerobic cultures.

scientific article published on 22 November 2007

Polyhydroxyalkanoates: Much More than Biodegradable Plastics.

scientific article

Pseudomonas 2.0: genetic upgrading of P. putida KT2440 as an enhanced host for heterologous gene expression.

scientific article published on 11 November 2014

Pseudomonas putida KT2440 Strain Metabolizes Glucose through a Cycle Formed by Enzymes of the Entner-Doudoroff, Embden-Meyerhof-Parnas, and Pentose Phosphate Pathways

scientific article published on 8 September 2015

Pseudomonas putida mt-2 tolerates reactive oxygen species generated during matric stress by inducing a major oxidative defense response.

scientific article published on 6 October 2015

Quantifying the Relative Importance of Phylogeny and Environmental Preferences As Drivers of Gene Content in Prokaryotic Microorganisms.

scientific article

Rapid Genome Engineering of Pseudomonas Assisted by Fluorescent Markers and Tractable Curing of Plasmids

publication published on 20 February 2021

Reconfiguration of metabolic fluxes in Pseudomonas putida as a response to sub-lethal oxidative stress

scientific article published on 11 January 2021

Redox driven metabolic tuning: carbon source and aeration affect synthesis of poly(3-hydroxybutyrate) in Escherichia coli.

scientific article published on 15 April 2010

Refactoring the Embden-Meyerhof-Parnas Pathway as a Whole of Portable GlucoBricks for Implantation of Glycolytic Modules in Gram-Negative Bacteria

scientific article published on 25 January 2017

Revolutionizing agriculture with synthetic biology

scientific article published on 18 November 2019

Robustness of Pseudomonas putida KT2440 as a host for ethanol biosynthesis

scientific article published on 23 February 2014

Statistical optimization of a culture medium for biomass and poly(3-hydroxybutyrate) production by a recombinant Escherichia coli strain using agroindustrial byproducts.

scientific article

Synergistic investigation of natural and synthetic C1-trophic microorganisms to foster a circular carbon economy

scientific article published in 2023

Synthesis of Recoded Bacterial Genomes toward Bespoke Biocatalysis

scientific article published on 13 July 2019

The CreC Regulator of Escherichia coli, a New Target for Metabolic Manipulations.

scientific article

The Entner-Doudoroff pathway empowers Pseudomonas putida KT2440 with a high tolerance to oxidative stress

scientific article

The Metabolic Redox Regime of Pseudomonas putida Tunes Its Evolvability toward Novel Xenobiotic Substrates

scientific article published in 2018

The RNA chaperone Hfq enables the environmental stress tolerance super-phenotype of Pseudomonas putida

scientific article published on 16 September 2015

The Standard European Vector Architecture (SEVA): a coherent platform for the analysis and deployment of complex prokaryotic phenotypes

scientific article published on 23 November 2012

The Synthetic Microbiology Caucus: from abstract ideas to turning microbes into cellular machines and back

scientific article published on 20 November 2018

The global regulator Crc orchestrates the metabolic robustness underlying oxidative stress resistance in Pseudomonas aeruginosa

scientific article published on 16 December 2018

The glycerol-dependent metabolic persistence of Pseudomonas putida KT2440 reflects the regulatory logic of the GlpR repressor.

scientific article

The metabolic cost of flagellar motion in Pseudomonas putida KT2440.

scientific article published on 18 November 2013

The private life of environmental bacteria: pollutant biodegradation at the single cell level

scientific article published on 16 January 2014

The revisited genome of Pseudomonas putida KT2440 enlightens its value as a robust metabolic chassis

scientific article

Transcriptomic fingerprinting of Pseudomonas putida under alternative physiological regimes

scientific article published on 5 September 2013

Why Nature Chose Potassium

scientific article published on 28 October 2019

Why are chlorinated pollutants so difficult to degrade aerobically? Redox stress limits 1,3-dichloroprop-1-ene metabolism by Pseudomonas pavonaceae

scientific article published on 11 March 2013

dye (arc) Mutants: insights into an unexplained phenotype and its suppression by the synthesis of poly (3-hydroxybutyrate) in Escherichia coli recombinants.

scientific article published in May 2006

List of works by Pablo I Nikel

A Metabolic Widget Adjusts the Phosphoenolpyruvate-Dependent Fructose Influx in Pseudomonas putida.

A New Player in the Biorefineries Field: Phasin PhaP Enhances Tolerance to Solvents and Boosts Ethanol and 1,3-Propanediol Synthesis in Escherichia coli.

A Post-translational Metabolic Switch Enables Complete Decoupling of Bacterial Growth from Biopolymer Production in Engineered Escherichia coli

A fluoride-responsive genetic circuit enables in vivo biofluorination in engineered Pseudomonas putida

Accelerated genome engineering of Pseudomonas putida by I-SceI-mediated recombination and CRISPR-Cas9 counterselection

Accumulation of inorganic polyphosphate enables stress endurance and catalytic vigour in Pseudomonas putida KT2440.

An expanded CRISPRi toolbox for tunable control of gene expression in Pseudomonas putida

Anr, the anaerobic global regulator, modulates the redox state and oxidative stress resistance in Pseudomonas extremaustralis

ArcA redox mutants as a source of reduced bioproducts.

ArsH protects Pseudomonas putida from oxidative damage caused by exposure to arsenic

Biochemistry, genetics and biotechnology of glycerol utilization in Pseudomonas species

Biotechnological domestication of pseudomonads using synthetic biology.

Biotransformation of 2,4-dinitrotoluene in a phototrophic co-culture of engineered Synechococcus elongatus and Pseudomonas putida

Breaking the state-of-the-art in the chemical industry with new-to-Nature products via synthetic microbiology

Chasing bacterial chassis for metabolic engineering: a perspective review from classical to non-traditional microorganisms

Combinatorial pathway balancing provides biosynthetic access to 2-fluoro-<i>cis</i>,<i>cis</i>-muconate in engineered <i>Pseudomonas putida</i>

Data on the standardization of a cyclohexanone-responsive expression system for Gram-negative bacteria

Dual Effect: High NADH Levels Contribute to Efflux-Mediated Antibiotic Resistance but Drive Lethality Mediated by Reactive Oxygen Species

Effects of granule-associated protein PhaP on glycerol-dependent growth and polymer production in poly(3-hydroxybutyrate)-producing Escherichia coli

Elimination of D-lactate synthesis increases poly(3-hydroxybutyrate) and ethanol synthesis from glycerol and affects cofactor distribution in recombinant Escherichia coli

Endogenous stress caused by faulty oxidation reactions fosters evolution of 2,4-dinitrotoluene-degrading bacteria

Engineering an anaerobic metabolic regime in Pseudomonas putida KT2440 for the anoxic biodegradation of 1,3-dichloroprop-1-ene ⬇️

Escherichia coli arcA mutants: metabolic profile characterization of microaerobic cultures using glycerol as a carbon source

Escherichia coli redox mutants as microbial cell factories for the synthesis of reduced biochemicals.

Ethanol synthesis from glycerol by Escherichia coli redox mutants expressing adhE from Leuconostoc mesenteroides.

Evolutionary Approaches for Engineering Industrially Relevant Phenotypes in Bacterial Cell Factories

Evolving metabolism of 2,4-dinitrotoluene triggers SOS-independent diversification of host cells

Exacerbation of substrate toxicity by IPTG in Escherichia coli BL21(DE3) carrying a synthetic metabolic pathway

Exploring the synthetic biology potential of bacteriophages for engineering non-model bacteria

Genetic programming of catalytic Pseudomonas putida biofilms for boosting biodegradation of haloalkanes

Genome reduction boosts heterologous gene expression in Pseudomonas putida

Growth-coupled selection of synthetic modules to accelerate cell factory development

High-throughput dilution-based growth method enables time-resolved exo-metabolomics of Pseudomonas putida and Pseudomonas aeruginosa

Implantation of unmarked regulatory and metabolic modules in Gram-negative bacteria with specialised mini-transposon delivery vectors ⬇️

Manipulation of the anoxic metabolism in Escherichia coli by ArcB deletion variants in the ArcBA two-component system

Metabolic and regulatory rearrangements underlying glycerol metabolism in Pseudomonas putida KT2440.

Metabolic selective pressure stabilizes plasmids carrying biosynthetic genes for reduced biochemicals in Escherichia coli redox mutants.

Modelling the freezing response of baker's yeast prestressed cells: a statistical approach.

Modular (de)construction of complex bacterial phenotypes by CRISPR/nCas9-assisted, multiplex cytidine base-editing

New recombinant Escherichia coli strain tailored for the production of poly(3-hydroxybutyrate) from agroindustrial by-products.

New transposon tools tailored for metabolic engineering of gram-negative microbial cell factories.

Non-invasive, ratiometric determination of intracellular pH in Pseudomonas species using a novel genetically encoded indicator

Physical decoupling of XylS/Pm regulatory elements and conditional proteolysis enable precise control of gene expression in Pseudomonas putida

Poly(3-hydroxybutyrate) synthesis by recombinant Escherichia coli arcA mutants in microaerobiosis.

Poly(3-hydroxybutyrate) synthesis from glycerol by a recombinant Escherichia coli arcA mutant in fed-batch microaerobic cultures.

Polyhydroxyalkanoates: Much More than Biodegradable Plastics.

Pseudomonas 2.0: genetic upgrading of P. putida KT2440 as an enhanced host for heterologous gene expression.

Pseudomonas putida KT2440 Strain Metabolizes Glucose through a Cycle Formed by Enzymes of the Entner-Doudoroff, Embden-Meyerhof-Parnas, and Pentose Phosphate Pathways

Pseudomonas putida mt-2 tolerates reactive oxygen species generated during matric stress by inducing a major oxidative defense response.

Quantifying the Relative Importance of Phylogeny and Environmental Preferences As Drivers of Gene Content in Prokaryotic Microorganisms.

Rapid Genome Engineering of <i>Pseudomonas</i> Assisted by Fluorescent Markers and Tractable Curing of Plasmids

Reconfiguration of metabolic fluxes in Pseudomonas putida as a response to sub-lethal oxidative stress

Redox driven metabolic tuning: carbon source and aeration affect synthesis of poly(3-hydroxybutyrate) in Escherichia coli.

Refactoring the Embden-Meyerhof-Parnas Pathway as a Whole of Portable GlucoBricks for Implantation of Glycolytic Modules in Gram-Negative Bacteria

Revolutionizing agriculture with synthetic biology

Robustness of Pseudomonas putida KT2440 as a host for ethanol biosynthesis

Statistical optimization of a culture medium for biomass and poly(3-hydroxybutyrate) production by a recombinant Escherichia coli strain using agroindustrial byproducts.

Synergistic investigation of natural and synthetic C1-trophic microorganisms to foster a circular carbon economy

Synthesis of Recoded Bacterial Genomes toward Bespoke Biocatalysis

The CreC Regulator of Escherichia coli, a New Target for Metabolic Manipulations.

The Entner-Doudoroff pathway empowers Pseudomonas putida KT2440 with a high tolerance to oxidative stress

The Metabolic Redox Regime of Pseudomonas putida Tunes Its Evolvability toward Novel Xenobiotic Substrates

The RNA chaperone Hfq enables the environmental stress tolerance super-phenotype of Pseudomonas putida

The Standard European Vector Architecture (SEVA): a coherent platform for the analysis and deployment of complex prokaryotic phenotypes

The Synthetic Microbiology Caucus: from abstract ideas to turning microbes into cellular machines and back

The global regulator Crc orchestrates the metabolic robustness underlying oxidative stress resistance in Pseudomonas aeruginosa

The glycerol-dependent metabolic persistence of Pseudomonas putida KT2440 reflects the regulatory logic of the GlpR repressor.

The metabolic cost of flagellar motion in Pseudomonas putida KT2440.

The private life of environmental bacteria: pollutant biodegradation at the single cell level

The revisited genome of Pseudomonas putida KT2440 enlightens its value as a robust metabolic chassis

Transcriptomic fingerprinting of Pseudomonas putida under alternative physiological regimes

Why Nature Chose Potassium

Why are chlorinated pollutants so difficult to degrade aerobically? Redox stress limits 1,3-dichloroprop-1-ene metabolism by Pseudomonas pavonaceae

dye (arc) Mutants: insights into an unexplained phenotype and its suppression by the synthesis of poly (3-hydroxybutyrate) in Escherichia coli recombinants.