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List of works by Nigel P Minton

13C-assisted metabolic flux analysis to investigate heterotrophic and mixotrophic metabolism in Cupriavidus necator H16.

scientific article published on 4 December 2017

A Gene System for Glucitol Transport and Metabolism in Clostridium beijerinckii NCIMB 8052 ⬇️

scientific article published on May 1, 1998

A Gold Standard, CRISPR/Cas9-Based Complementation Strategy Reliant on 24 Nucleotide Bookmark Sequences

scientific article published on 23 April 2020

A Sustainable Chemicals Manufacturing Paradigm Using CO2 and Renewable H2

scientific article published on 02 June 2020

A Transcription Factor-Based Biosensor for Detection of Itaconic Acid.

scientific article

A genetic assay for gene essentiality in Clostridium

scientific article published on 31 July 2016

A genome-wide approach for identification and characterisation of metabolite-inducible systems

scientific article published on 05 March 2020

A mariner-based transposon system for in vivo random mutagenesis of Clostridium difficile

scientific article

A modular system for Clostridium shuttle plasmids

scientific article

A novel approach to generate a recombinant toxoid vaccine against Clostridium difficile.

scientific article published on 29 April 2013

A novel conjugal donor strain for improved DNA transfer into Clostridium spp

scientific article published on 30 June 2019

A roadmap for gene system development in Clostridium.

scientific article published on 24 May 2016

A strategy for mapping and neutralizing conformational immunogenic sites on protein therapeutics

scientific article published in March 2002

A universal mariner transposon system for forward genetic studies in the genus Clostridium

scientific article

Advances in metabolic engineering in the microbial production of fuels and chemicals from C1 gas.

scientific article published on 31 January 2018

Advancing Clostridia to Clinical Trial: Past Lessons and Recent Progress

scientific article

Anaerobic bacteria as a delivery system for cancer gene therapy: in vitro activation of 5-fluorocytosine by genetically engineered clostridia.

scientific article

Anaerobic bacteria as a gene delivery system that is controlled by the tumor microenvironment

scientific article

AnagrQuorum Sensing System That Regulates Granulose Formation and Sporulation in Clostridium acetobutylicum ⬇️

scientific article published on December 16, 2011

Anticancer efficacy of systemically delivered anaerobic bacteria as gene therapy vectors targeting tumor hypoxia/necrosis

scientific article published in February 2002

Applying asymptotic methods to synthetic biology: Modelling the reaction kinetics of the mevalonate pathway.

scientific article published on 30 November 2017

Array comparative hybridisation reveals a high degree of similarity between UK and European clinical isolates of hypervirulent Clostridium difficile.

scientific article

Bacillus amyloliquefaciens orthologue of Bacillus subtilis ywrO encodes a nitroreductase enzyme which activates the prodrug CB 1954.

scientific article published in January 2002

Binding of the anticancer prodrug CB1954 to the activating enzyme NQO2 revealed by the crystal structure of their complex

scientific article

Both, toxin A and toxin B, are important in Clostridium difficile infection ⬇️

scientific article published on July 1, 2011

CRISPR-Cas9D10A nickase-assisted base editing in the solvent producer Clostridium beijerinckii

scientific article published on 21 February 2019

CRISPR-based genome editing and expression control systems in Clostridium acetobutylicum and Clostridium beijerinckii.

scientific article published on 23 May 2016

CRISPR/Cas9-Based Efficient Genome Editing in Clostridium ljungdahlii, an Autotrophic Gas-Fermenting Bacterium

scientific article published on 8 June 2016

Cellular Uptake and Mode-of-Action of Clostridium difficile Toxins

scientific article published in January 2018

Characterisation of a 3-hydroxypropionic acid-inducible system from Pseudomonas putida for orthogonal gene expression control in Escherichia coli and Cupriavidus necator.

scientific article published on 11 May 2017

Characterization of a region of the Enterococcus faecalis plasmid pAM beta 1 which enhances the segregational stability of pAM beta 1-derived cloning vectors in Bacillus subtilis

scientific article published in November 1991

Characterization of the impact of rpoB mutations on the in vitro and in vivo competitive fitness of Clostridium difficile and susceptibility to fidaxomicin

scientific article published on 15 December 2017

Chemotherapeutic tumour targeting using clostridial spores

scientific article

Cloning and complete nucleotide sequence of the Bacillus stearothermophilus tryptophanyl tRNA synthetase gene

scientific article

Cloning and expression of the Erwinia chrysanthemi asparaginase gene in Escherichia coli and Erwinia carotovora.

scientific article

Cloning and nucleotide sequences of the mdh and sucD genes from Thermus aquaticus B.

scientific article published on June 1990

Cloning and sequence analysis of the genes encoding phosphotransbutyrylase and butyrate kinase from Clostridium acetobutylicum NCIMB 8052.

scientific article

Cloning enzyme genes for overproduction

scientific article published on 01 April 1984

Cloning, expression and complete nucleotide sequence of the Bacillus stearothermophilus L-lactate dehydrogenase gene

scientific article

Cloning, expression and evaluation of a recombinant sub-unit vaccine against Clostridium botulinum type F toxin

scientific article published on 01 September 2000

Cloning, sequencing, and expression in Escherichia coli of the Clostridium tetanomorphum gene encoding beta-methylaspartase and characterization of the recombinant protein

scientific article published on November 1992

ClosTron-mediated engineering of Clostridium

scientific article published on July 2012

ClosTron-mediated engineering of Clostridium

scientific article published on 01 January 2011

ClosTron-targeted mutagenesis

scientific article published on 01 January 2010

Closed Genome Sequence of Clostridium pasteurianum ATCC 6013.

scientific article published on 19 February 2015

Clostridia in cancer therapy

scientific article

Clostridium difficile Genome Editing Using pyrE Alleles.

scientific article published in January 2016

Clostridium difficile modulates host innate immunity via toxin-independent and dependent mechanism(s).

scientific article

Clostridium difficile secreted Pro-Pro endopeptidase PPEP-1 (ZMP1/CD2830) modulates adhesion through cleavage of the collagen binding protein CD2831.

scientific article published on 29 October 2015

Clostridium difficile spore germination: an update.

scientific article published on 21 September 2010

Clostridium difficile-mediated effects on human intestinal epithelia: Modelling host-pathogen interactions in a vertical diffusion chamber.

scientific article published on 18 December 2015

Coenzyme A-transferase-independent butyrate re-assimilation in Clostridium acetobutylicum-evidence from a mathematical model

scientific article published on 23 August 2014

Coinfection and Emergence of Rifamycin Resistance during a Recurrent Clostridium difficile Infection

scientific article published on 24 August 2016

Comparison of culture based methods for the isolation of Clostridium difficile from stool samples in a research setting

scientific article

Complete Genome Sequence of Cupriavidus necator H16 (DSM 428)

scientific article published on 12 September 2019

Complete Genome Sequence of Geobacillus thermoglucosidasius NCIMB 11955, the Progenitor of a Bioethanol Production Strain

scientific article

Complete Genome Sequence of the Nitrogen-Fixing and Solvent-Producing Clostridium pasteurianum DSM 525

scientific article published on 19 February 2015

Complete Genome Sequence of the Nonpathogenic Soil-Dwelling Bacterium Clostridium sporogenes Strain NCIMB 10696.

scientific article published on 20 August 2015

Complete nucleotide sequence of the Rhodosporidium toruloides gene coding for phenylalanine ammonia-lyase

scientific article published in January 1987

Conjugative transfer of clostridial shuttle vectors from Escherichia coli to Clostridium difficile through circumvention of the restriction barrier

scientific article published in October 2002

Construction of a nontoxigenic Clostridium botulinum strain for food challenge studies

scientific article published on 20 November 2009

Design, cloning and characterization of transcription factor-based inducible gene expression systems

scientific article published on 06 March 2019

Development and implementation of rapid metabolic engineering tools for chemical and fuel production in Geobacillus thermoglucosidasius NCIMB 11955

scientific article

Development of Clostridium difficile R20291ΔPaLoc model strains and in vitro methodologies reveals CdtR is required for the production of CDT to cytotoxic levels.

scientific article

Development of a transformation and gene reporter system for group II, non-proteolytic Clostridium botulinum type B strains.

scientific article published in January 2000

Development of an inducible transposon system for efficient random mutagenesis in Clostridium acetobutylicum

scientific article

Development of an integrative vector for the expression of antisense RNA in Clostridium difficile.

scientific article published in December 2003

Disruption of the acetate kinase (ack) gene of Clostridium acetobutylicum results in delayed acetate production

scientific article (publication date: May 2012)

Effect of antibiotic treatment on the formation of non-spore Clostridium difficile persister-like cells

scientific article published on 01 September 2018

Engineering of vitamin prototrophy in Clostridium ljungdahlii and Clostridium autoethanogenum

scientific article published on 10 April 2019

Enhanced solvent production by metabolic engineering of a twin-clostridial consortium

scientific article published on 26 October 2016

Entry of spores into intestinal epithelial cells contributes to recurrence of Clostridioides difficile infection

scientific article published on 18 February 2021

Establishing Mixotrophic Growth of Cupriavidus necator H16 on CO2 and Volatile Fatty Acids

scientific article published in 2022

Expanding the repertoire of gene tools for precise manipulation of the Clostridium difficile genome: allelic exchange using pyrE alleles

scientific article

Expression of the Pseudomonas gene coding for carboxypeptidase G2 in Saccharomyces cerevisiae.

scientific article

Expression of the bacterial nitroreductase enzyme in mammalian cells renders them selectively sensitive to killing by the prodrug CB1954.

scientific article published on December 1995

Femtomolar sensitivity of a NO sensor from Clostridium botulinum.

scientific article published on 7 October 2004

Fluoroquinolone resistance does not impose a cost on the fitness of Clostridium difficile in vitro

scientific article published on 22 December 2014

Functional Genetic Elements for Controlling Gene Expression in Cupriavidus necator H16

article

Functional Intestinal Bile Acid 7α-Dehydroxylation by Clostridium scindens Associated with Protection from Clostridium difficile Infection in a Gnotobiotic Mouse Model

scientific article (publication date: 2016)

Gene transfer into Clostridium difficile CD630 and characterisation of its methylase genes.

scientific article

Generation of a fully erythromycin-sensitive strain of Clostridioides difficile using a novel CRISPR-Cas9 genome editing system.

scientific article published on 31 May 2019

Genetic characterisation of the botulinum toxin complex of Clostridium botulinum strain NCTC 2916

scientific journal article

Genetically modified clostridium for gene therapy of tumors

scientific article published in January 2000

Genome Sequence of the Solvent-Producing Clostridium beijerinckii Strain 59B, Isolated from Staffordshire Garden Soil

scientific article

Genome sequence of a proteolytic (Group I) Clostridium botulinum strain Hall A and comparative analysis of the clostridial genomes

scientific article published on 22 May 2007

Genome sequencing shows that European isolates of Francisella tularensis subspecies tularensis are almost identical to US laboratory strain Schu S4.

scientific article

Heterologous gene expression in the human gut bacteria Eubacterium rectale and Roseburia inulinivorans by means of conjugative plasmids

scientific article published on 19 June 2019

High-level expression of the phenylalanine ammonia lyase-encoding gene from Rhodosporidium toruloides in Saccharomyces cerevisiae and Escherichia coli using a bifunctional expression system.

scientific article

Homologous overexpression of hydrogenase and glycerol dehydrogenase in Clostridium pasteurianum to enhance hydrogen production from crude glycerol

scientific article published on 16 March 2019

Identification of the promoter of the Pseudomonas gene coding for carboxypeptidase G2.

scientific article published in January 1985

Important role of class I heat shock genes hrcA and dnaK in the heat shock response and the response to pH and NaCl stress of group I Clostridium botulinum strain ATCC 3502

scientific journal article

Improved plasmid vectors for the isolation of translational lac gene fusions ⬇️

scientific article published on 01 November 1984

Improving gene transfer in Clostridium pasteurianum through the isolation of rare hypertransformable variants

scientific article published on 9 September 2017

Improving the reproducibility of the NAP1/B1/027 epidemic strain R20291 in the hamster model of infection.

scientific article

In vitro and in vivo characterisation of a recombinant carboxypeptidase G2::anti-CEA scFv fusion protein ⬇️

scientific article published on February 1, 1996

Inactivation of the dnaK gene in Clostridium difficile 630 Δerm yields a temperature-sensitive phenotype and increases biofilm-forming ability

scientific article published on 13 December 2017

Inflammasome activation contributes to interleukin-23 production in response to Clostridium difficile

scientific article

Insights into CO2 Fixation Pathway of Clostridium autoethanogenum by Targeted Mutagenesis

scientific article

Integration of DNA into bacterial chromosomes from plasmids without a counter-selection marker

scientific article published on 18 January 2012

Introduction of genes for leucine biosynthesis from Clostridium pasteurianum into C. acetobutylicum by cointegrate conjugal transfer

scientific article published on 01 September 1988

Involvement of two-component system CBO0366/CBO0365 in the cold shock response and growth of group I (proteolytic) Clostridium botulinum ATCC 3502 at low temperatures

scientific article published on June 2012

Lipoprotein CD0873 is a novel adhesin of Clostridium difficile

scientific article published on 29 January 2014

Metabolic engineering of Clostridium autoethanogenum for selective alcohol production.

scientific article published on 19 January 2017

Microbial solvent formation revisited by comparative genome analysis

scientific article

Modifying an immunogenic epitope on a therapeutic protein: a step towards an improved system for antibody-directed enzyme prodrug therapy (ADEPT)

scientific article published on June 2004

Molecular analysis of a Clostridium butyricum NCIMB 7423 gene encoding 4-α-glucanotransferase and characterization of the recombinant enzyme produced in Escherichia coli ⬇️

scientific article published on October 1, 1997

Molecular analysis of the malR gene of Clostridium butyricum NCIMB 7423, a member of the LacI-GalR family of repressor proteins

scientific article published in August 1998

Molecular cloning and nucleotide sequence determination of the Bacillus stearothermophilus NCA 1503 superoxide dismutase gene and its overexpression in Escherichia coli

scientific article published on December 1991

Molecular cloning of the Clostridium botulinum structural gene encoding the type B neurotoxin and determination of its entire nucleotide sequence.

scientific article

Molecular cloning of the Pseudomonas carboxypeptidase G2 gene and its expression in Escherichia coli and Pseudomonas putida ⬇️

scientific article published on December 1, 1983

Multi-timescale analysis of a metabolic network in synthetic biology: a kinetic model for 3-hydroxypropionic acid production via beta-alanine.

scientific article

Multiple factors modulate biofilm formation by the anaerobic pathogen Clostridium difficile

scientific article published on 21 November 2012

Multiple orphan histidine kinases interact directly with Spo0A to control the initiation of endospore formation in Clostridium acetobutylicum

scientific article

Mutant generation by allelic exchange and genome resequencing of the biobutanol organism Clostridium acetobutylicum ATCC 824

scientific article

Nucleoside Diphosphate Sugar Hydrolase Gene of Salmonella typhimurium : Chromosomal Location Determined by Intergeneric Crosses ⬇️

scientific article published on March 1, 1979

Nucleotide sequence analysis of the gene for protein A from Staphylococcus aureus Cowan 1 (NCTC8530) and its enhanced expression in Escherichia coli

scientific article published in January 1987

Nucleotide sequence of an Erwinia chrysanthemi gene encoding shikimate kinase

scientific article published on February 1989

Nucleotide sequence of the Erwinia chrysanthemi NCPPB 1066 L-asparaginase gene

scientific article published in January 1986

Optimal spore germination in Clostridium botulinum ATCC 3502 requires the presence of functional copies of SleB and YpeB, but not CwlJ.

scientific article published on 29 April 2015

Pharmacokinetic and pharmacodynamic modeling of recombinant human erythropoietin after single and multiple doses in healthy volunteers

scientific article published in September 2004

Phosphorylation and functionality of CdtR in Clostridium difficile

scientific article published on 16 July 2019

Physical characterisation and over-expression of the Bacillus caldotenax superoxide dismutase gene

scientific article published on March 1992

Physical characterisation of the Escherichia coli B gene encoding nitroreductase and its over-expression in Escherichia coli K12.

scientific article published on December 1994

Physical characterisation of the replication region of the Streptococcus faecalis plasmid pAM beta 1.

scientific article published on March 1990

Physical characterization of the replication origin of the cryptic plasmid pCB101 isolated from Clostridium butyricum NCIB 7423.

scientific article published on July 1992

Precise manipulation of the Clostridium difficile chromosome reveals a lack of association between the tcdC genotype and toxin production.

scientific article published on 20 April 2012

Production of a functional cell wall-anchored minicellulosome by recombinant Clostridium acetobutylicum ATCC 824

scientific article

Progress towards platform chemical production using

scientific article published on 17 April 2018

Proposed topology of the glucitol permeases of Escherichia coli and Clostridium acetobutylicum

scientific article published in November 1996

Quantitative Isotope-Dilution High-Resolution-Mass-Spectrometry Analysis of Multiple Intracellular Metabolites in Clostridium autoethanogenum with Uniformly 13C-Labeled Standards Derived from Spirulina

scientific article published on 21 March 2018

Quorum sensing in Clostridium difficile: analysis of a luxS-type signalling system.

scientific article published in February 2005

Rapid small-scale isolation of mRNA from whole yeast cells.

scientific article

Recombinant expression analysis of natural and synthetic bovine alpha-casein in Escherichia coli.

scientific article published in November 2000

Recombinant expression and characterisation of the oxygen-sensitive 2-enoate reductase from Clostridium sporogenes

scientific article published on 7 November 2017

Reconsidering the sporulation characteristics of hypervirulent Clostridium difficile BI/NAP1/027.

scientific article

Regulation of cel genes of C. cellulolyticum: identification of GlyR2, a transcriptional regulator regulating cel5D gene expression

scientific article

Regulation of lactate metabolism in the acetogenic bacterium Acetobacterium woodii

scientific article published on 24 October 2018

Regulation of neurotoxin production and sporulation by a Putative agrBD signaling system in proteolytic Clostridium botulinum.

scientific article published on 7 May 2010

Release of TcdA and TcdB from Clostridium difficile cdi 630 is not affected by functional inactivation of the tcdE gene.

scientific article

Repeated cycles of Clostridium-directed enzyme prodrug therapy result in sustained antitumour effects in vivo

scientific article published on 3 October 2006

RiboCas: A Universal CRISPR-Based Editing Tool for Clostridium

scientific article published on 07 June 2019

Riboswitch (T-box)-mediated control of tRNA-dependent amidation in Clostridium acetobutylicum rationalizes gene and pathway redundancy for asparagine and asparaginyl-trnaasn synthesis.

scientific article

SBRC-Nottingham: sustainable routes to platform chemicals from C1 waste gases

scientific article

Secretion and assembly of functional mini-cellulosomes from synthetic chromosomal operons in Clostridium acetobutylicum ATCC 824

scientific article (publication date: 20 August 2013)

Sequence of the gene for alkaline phosphatase from Escherichia coli JM83.

scientific article

Spores of Clostridium difficile clinical isolates display a diverse germination response to bile salts

scientific article

Spores of Clostridium engineered for clinical efficacy and safety cause regression and cure of tumors in vivo

scientific article

Sporulation studies in Clostridium difficile

scientific article

Sustained tumor regression of human colorectal cancer xenografts using a multifunctional mannosylated fusion protein in antibody-directed enzyme prodrug therapy.

scientific article published in January 2005

Syngas Biorefinery and Syngas Utilization.

scientific article published on 20 June 2017

Synthetic Biology on Acetogenic Bacteria for Highly Efficient Conversion of C1 Gases to Biochemicals

scientific article published on 15 October 2020

Targeted mutagenesis of the Clostridium acetobutylicum acetone-butanol-ethanol fermentation pathway.

scientific article

The Butanol Producing Microbe Clostridium beijerinckii NCIMB 14988 Manipulated Using Forward and Reverse Genetic Tools

scientific article published on 16 April 2018

The ClosTron: Mutagenesis in Clostridium refined and streamlined.

scientific article published on 3 November 2009

The ClosTron: a universal gene knock-out system for the genus Clostridium

scientific article published on 18 June 2007

The R-factors of multiple antibiotic resistant faecal coliforms isolated from a domestic dog.

scientific article

The Rnf Complex Is an Energy-Coupled Transhydrogenase Essential To Reversibly Link Cellular NADH and Ferredoxin Pools in the Acetogen Acetobacterium woodii

scientific article published on 10 October 2018

The SOS Response Master Regulator LexA Is Associated with Sporulation, Motility and Biofilm Formation in Clostridium difficile

scientific article published on 18 December 2015

The analysis of para-cresol production and tolerance in Clostridium difficile 027 and 012 strains

scientific article

The binary toxin CDT enhances Clostridium difficile virulence by suppressing protective colonic eosinophilia

scientific article

The cold-induced two-component system CBO0366/CBO0365 regulates metabolic pathways with novel roles in group I Clostridium botulinum ATCC 3502 cold tolerance

scientific article published on 25 October 2013

The complete amino acid sequence of the Clostridium botulinum type A neurotoxin, deduced by nucleotide sequence analysis of the encoding gene

scientific article published on April 1990

The complete amino acid sequence of the Clostridium botulinum type-E neurotoxin, derived by nucleotide-sequence analysis of the encoding gene

scientific article published on March 1992

The complete nucleotide sequence of the Pseudomonas gene coding for carboxypeptidase G2.

scientific article published in November 1984

The development of Clostridium difficile genetic systems.

scientific article

The diverse sporulation characteristics of Clostridium difficile clinical isolates are not associated with type

scientific article published on 13 October 2010

The flagellin FliC of Clostridium difficile is responsible for pleiotropic gene regulation during in vivo infection

scientific article

The glucosyltransferase activity of C. difficile Toxin B is required for disease pathogenesis

scientific article published on 22 September 2020

The multidrug-resistant human pathogen Clostridium difficile has a highly mobile, mosaic genome

scientific article

The nucleotide sequence of genes involved in the leucine biosynthetic pathway of Clostridium pasteurianum

scientific article published on January 1993

The pMTL nic- cloning vectors. I. Improved pUC polylinker regions to facilitate the use of sonicated DNA for nucleotide sequencing

scientific article published in August 1988

The potential of clostridial spores as therapeutic delivery vehicles in tumour therapy.

scientific article published on 07 January 2015

The putative influence of the agr operon upon survival mechanisms used by Clostridium acetobutylicum

scientific article published on 26 March 2013

The replication proteins of plasmids pE194 and pLS1 have N-terminal homology ⬇️

scientific article published on April 11, 1988

The role of flagella in Clostridium difficile pathogenesis: comparison between a non-epidemic and an epidemic strain

scientific article

The role of flagella in Clostridium difficile pathogenicity

scientific article published on 04 February 2015

The role of small acid-soluble proteins (SASPs) in protection of spores of Clostridium botulinum against nitrous acid.

scientific article published on 3 September 2015

The role of toxin A and toxin B in Clostridium difficile infection

scientific article

Towards improved butanol production through targeted genetic modification of Clostridium pasteurianum.

scientific article published on 21 January 2017

Two-component signal transduction system CBO0787/CBO0786 represses transcription from botulinum neurotoxin promoters in Clostridium botulinum ATCC 3502.

scientific article

Use of an optimised enzyme/prodrug combination for Clostridia directed enzyme prodrug therapy induces a significant growth delay in necrotic tumours

scientific article published on 8 February 2021

Using singular perturbation theory to determine kinetic parameters in a non-standard coupled enzyme assay

scientific article published on 06 August 2020

Variability in Arsenic Methylation Efficiency across Aerobic and Anaerobic Microorganisms

scientific article published on 30 October 2020

What's a SNP between friends: The influence of single nucleotide polymorphisms on virulence and phenotypes of Clostridium difficile strain 630 and derivatives.

scientific article published on 21 September 2016

Whole genome sequence and manual annotation of Clostridium autoethanogenum, an industrially relevant bacterium

scientific article

cspB encodes a major cold shock protein in Clostridium botulinum ATCC 3502.

scientific article

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