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List of works by Jon R. Lorsch

A conformational change in the eukaryotic translation preinitiation complex and release of eIF1 signal recognition of the start codon

scientific article

A mechanistic overview of translation initiation in eukaryotes ⬇️

scientific article published on June 5, 2012

ATP and GTP hydrolysis assays (TLC).

scientific article published on January 2013

Active yeast ribosome preparation using monolithic anion exchange chromatography

scientific article published on 16 December 2016

Basic science: Bedrock of progress

scientific article published on March 2016

Cell Biology. Fixing problems with cell lines

scientific article published on December 2014

Communication between eukaryotic translation initiation factors 1 and 1A on the yeast small ribosomal subunit

scientific article

Communication between eukaryotic translation initiation factors 5 and 1A within the ribosomal pre-initiation complex plays a role in start site selection

scientific article

Conformational Differences between Open and Closed States of the Eukaryotic Translation Initiation Complex

scientific article

Conserved residues in yeast initiator tRNA calibrate initiation accuracy by regulating preinitiation complex stability at the start codon

scientific article published on March 2014

Coordinated movements of eukaryotic translation initiation factors eIF1, eIF1A, and eIF5 trigger phosphate release from eIF2 in response to start codon recognition by the ribosomal preinitiation complex

scientific article published on 4 January 2013

Coupled release of eukaryotic translation initiation factors 5B and 1A from 80S ribosomes following subunit joining.

scientific article

Development and characterization of a reconstituted yeast translation initiation system.

scientific article

Dissociation of eIF1 from the 40S ribosomal subunit is a key step in start codon selection in vivo

scientific article

Distinct interactions of eIF4A and eIF4E with RNA helicase Ded1 stimulate translation in vivo

scientific article published on 29 May 2020

Enhanced eIF1 binding to the 40S ribosome impedes conformational rearrangements of the preinitiation complex and elevates initiation accuracy.

scientific article published on 13 December 2013

Eukaryotic initiator tRNA: finely tuned and ready for action

scientific article

Eukaryotic translation initiation factor 3 plays distinct roles at the mRNA entry and exit channels of the ribosomal preinitiation complex.

scientific article

Eukaryotic translation initiation factor eIF5 promotes the accuracy of start codon recognition by regulating Pi release and conformational transitions of the preinitiation complex

scientific article

Eukaryotic translation initiation factor eIF5 promotes the accuracy of start codon recognition by regulating Pi release and conformational transitions of the preinitiation complex.

scientific article published on 18 May 2015

Explanatory chapter: nucleic acid concentration determination

scientific article published in January 2013

Genetic identification of yeast 18S rRNA residues required for efficient recruitment of initiator tRNA(Met) and AUG selection

scientific article published on August 2008

Identification and characterization of functionally critical, conserved motifs in the internal repeats and N-terminal domain of yeast translation initiation factor 4B (yeIF4B)

scientific article

Identification and characterization of functionally critical, conserved motifs in the internal repeats and N-terminal domain of yeast translation initiation factor 4B (yeIF4B).

scientific article published in April 2014

Identification of compounds that decrease the fidelity of start codon recognition by the eukaryotic translational machinery

scientific article

Initiation factor eIF2γ promotes eIF2-GTP-Met-tRNAi(Met) ternary complex binding to the 40S ribosome

scientific article published on 16 October 2011

Initiation of Protein Synthesis by Hepatitis C Virus Is Refractory to Reduced eIF2 · GTP · Met-tRNAiMet Ternary Complex Availability

scientific article (publication date: November 2006)

Interaction between eukaryotic initiation factors 1A and 5B is required for efficient ribosomal subunit joining.

scientific article

Intragenic suppressor mutations restore GTPase and translation functions of a eukaryotic initiation factor 5B switch II mutant

scientific article published on 22 December 2006

Kinetic analysis of late steps of eukaryotic translation initiation

scientific article published on 19 October 2008

Kinetic and thermodynamic analysis of the role of start codon/anticodon base pairing during eukaryotic translation initiation

scientific article published on 24 November 2008

Labeling a protein with fluorophores using NHS ester derivitization

scientific article published on 01 January 2014

Labeling of a protein with fluorophores using maleimide derivitization

scientific article published on 01 January 2014

Maximizing the return on taxpayers' investments in fundamental biomedical research

scientific article published on May 2015

Mechanism of ribosomal subunit joining during eukaryotic translation initiation.

scientific article published on August 2008

Methods in Enzymology. Laboratory methods in enzymology: DNA. Preface

scientific article published in January 2013

Methods in Enzymology. Laboratory methods in enzymology: RNA. Preface

scientific article published on 01 January 2013

Methods in Enzymology. Laboratory methods in enzymology: protein part A. Preface

scientific article published on 01 January 2014

Methods in Enzymology. Laboratory methods in enzymology: protein part B. Preface

scientific article published on 01 January 2014

Molecular architecture of a eukaryotic translational initiation complex

scientific article

Molecular view of 43 S complex formation and start site selection in eukaryotic translation initiation

scientific article

Multiple elements in the eIF4G1 N-terminus promote assembly of eIF4G1•PABP mRNPs in vivo.

scientific article

N- and C-terminal residues of eIF1A have opposing effects on the fidelity of start codon selection

scientific article published on March 2007

Organizing graduate life sciences education around nodes and connections

scientific article published on 01 August 2011

Perspective: Sustaining the big-data ecosystem

scientific article

Pi release from eIF2, not GTP hydrolysis, is the step controlled by start-site selection during eukaryotic translation initiation

scientific article

Practical steady-state enzyme kinetics

scientific article published on January 2014

Preface. Laboratory Methods in Enzymology: Protein Part D

scientific article published on 01 January 2015

Preface. Laboratory methods in enzymology: cell, lipid and carbohydrate.

scientific article

Protein Affinity Purification using Intein/Chitin Binding Protein Tags

scientific article published on 16 May 2015

Protein derivitization-expressed protein ligation

scientific article published on 01 January 2014

Protein filter binding

scientific article published in January 2014

RNA Purification – Precipitation Methods

scientific article published in 2013

RNA chaperones exist and DEAD box proteins get a life

scientific article

Reconstitution of yeast translation initiation.

scientific article published in January 2007

Regulatory elements in eIF1A control the fidelity of start codon selection by modulating tRNA(i)(Met) binding to the ribosome

scientific article

Reverse transcriptase dideoxy sequencing of RNA.

scientific article

Ribosome recycling step in yeast cytoplasmic protein synthesis is catalyzed by eEF3 and ATP.

scientific article

Ribozyme catalysis: not different, just worse

scientific article

Rps3/uS3 promotes mRNA binding at the 40S ribosome entry channel and stabilizes preinitiation complexes at start codons

scientific article published on 21 February 2017

Sanger dideoxy sequencing of DNA.

scientific article

Should I stay or should I go? Eukaryotic translation initiation factors 1 and 1A control start codon recognition

scientific article published on 30 June 2008

Specific domains in yeast translation initiation factor eIF4G strongly bias RNA unwinding activity of the eIF4F complex toward duplexes with 5'-overhangs

scientific article

Standard in vitro assays for protein-nucleic acid interactions--gel shift assays for RNA and DNA binding

scientific article published in January 2014

Structural changes enable start codon recognition by the eukaryotic translation initiation complex

scientific article

Structural integrity of {alpha}-helix H12 in translation initiation factor eIF5B is critical for 80S complex stability.

scientific article

Temperature-dependent regulation of upstream open reading frame translation in S. cerevisiae

scientific article published on 06 December 2019

The 5'-7-methylguanosine cap on eukaryotic mRNAs serves both to stimulate canonical translation initiation and to block an alternative pathway.

scientific article

The C-terminal domain of eukaryotic initiation factor 5 promotes start codon recognition by its dynamic interplay with eIF1 and eIF2β

scientific article

The DEAD box protein eIF4A. 1. A minimal kinetic and thermodynamic framework reveals coupled binding of RNA and nucleotide

scientific article published in February 1998

The DEAD box protein eIF4A. 2. A cycle of nucleotide and RNA-dependent conformational changes

scientific article published in February 1998

The eIF1A C-terminal domain promotes initiation complex assembly, scanning and AUG selection in vivo

scientific article

The eukaryotic translation initiation factors eIF1 and eIF1A induce an open conformation of the 40S ribosome

scientific article

The mechanism of eukaryotic translation initiation: new insights and challenges

scientific article

The mercury resistance (mer) operon in a marine gliding flavobacterium,Tenacibaculum discolor9A5 ⬇️

scientific article published on August 20, 2012

The molecular mechanics of eukaryotic translation

scientific article

The path to perdition is paved with protons

scientific article

Translational initiation factor eIF5 replaces eIF1 on the 40S ribosomal subunit to promote start-codon recognition

scientific article published on 30 November 2018

Uncoupling of initiation factor eIF5B/IF2 GTPase and translational activities by mutations that lower ribosome affinity

scientific article

Where to begin? The mechanism of translation initiation codon selection in eukaryotes

scientific article published on 28 August 2006

Yeast Ded1 promotes 48S translation pre-initiation complex assembly in an mRNA-specific and eIF4F-dependent manner

scientific article

Yeast eIF4A enhances recruitment of mRNAs regardless of their structural complexity.

scientific article published on 30 November 2017

Yeast eIF4B binds to the head of the 40S ribosomal subunit and promotes mRNA recruitment through its N-terminal and internal repeat domains

scientific article

Yeast eukaryotic initiation factor 4B (eIF4B) enhances complex assembly between eIF4A and eIF4G in vivo

scientific article

eIF1 controls multiple steps in start codon recognition during eukaryotic translation initiation

scientific article published on 12 September 2009

eIF1A residues implicated in cancer stabilize translation preinitiation complexes and favor suboptimal initiation sites in yeast.

scientific article

rRNA suppressor of a eukaryotic translation initiation factor 5B/initiation factor 2 mutant reveals a binding site for translational GTPases on the small ribosomal subunit.

scientific article

β-Hairpin loop of eukaryotic initiation factor 1 (eIF1) mediates 40 S ribosome binding to regulate initiator tRNA(Met) recruitment and accuracy of AUG selection in vivo

scientific article published on 26 July 2013

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