List of works - David F. Ackerley

A cofactor consumption screen identifies promising NfsB family nitroreductases for dinitrotoluene remediation

scientific article published on 07 August 2019

A functional screen for recovery of 4'-phosphopantetheinyl transferase and associated natural product biosynthesis genes from metagenome libraries.

scientific article

A gain-of-function positive-selection expression plasmid that enables high-efficiency cloning

scientific article

A giant leap in sequence space reveals the intracellular complexities of evolving a new function

A metagenomic library cloning strategy that promotes high-level expression of captured genes to enable efficient functional screening

scientific article published on 25 March 2023

A sensitive single-enzyme assay system using the non-ribosomal peptide synthetase BpsA for measurement of L-glutamine in biological samples

scientific article published on 31 January 2017

Advancing Clostridia to Clinical Trial: Past Lessons and Recent Progress

scientific article

Bioactivity-Guided Metabolite Profiling of Feijoa ( Acca sellowiana) Cultivars Identifying 4-Cyclopentene-1,3-dione as a Potent Antifungal Inhibitor of Chitin Synthesis.

scientific article published on 16 March 2018

Biosynthesis of novel Pyoverdines by domain substitution in a nonribosomal peptide synthetase of Pseudomonas aeruginosa

scientific article published on 11 July 2014

Characterization and genetic manipulation of peptide synthetases in Pseudomonas aeruginosa PAO1 in order to generate novel pyoverdines

scientific journal article

Characterization of pyoverdine and achromobactin in Pseudomonas syringae pv. phaseolicola 1448a

scientific article

ChrR, a soluble quinone reductase of Pseudomonas putida that defends against H2O2.

scientific article

Chromate-reducing properties of soluble flavoproteins from Pseudomonas putida and Escherichia coli.

scientific article

Correction to Lamellarin Sulfates from the Pacific Tunicate Didemnum ternerratum

scientific article published on 23 September 2020

Cracking the Nonribosomal Code

scientific article published in May 2016

Creation and screening of a multi-family bacterial oxidoreductase library to discover novel nitroreductases that efficiently activate the bioreductive prodrugs CB1954 and PR-104A.

scientific article

Crystal structure of the essential Mycobacterium tuberculosis phosphopantetheinyl transferase PptT, solved as a fusion protein with maltose binding protein

scientific article

Development of a Mycobacterium smegmatis transposon mutant array for characterising the mechanism of action of tuberculosis drugs: Findings with isoniazid and its structural analogues

article

Directed Evolution of the Nonribosomal Peptide Synthetase BpsA to Enable Recognition by the Human Phosphopantetheinyl Transferases for Counter-Screening Antibiotic Candidates

scientific article published on 29 October 2020

Directed evolution of the B. subtilis nitroreductase YfkO improves activation of the PET-capable probe SN33623 and CB1954 prodrug

scientific article published on 26 August 2020

Discovery and evaluation of Escherichia coli nitroreductases that activate the anti-cancer prodrug CB1954.

scientific article

Effect of chromate stress on Escherichia coli K-12.

scientific article published on May 2006

Efficient rational modification of non-ribosomal peptides by adenylation domain substitution

scientific article published on 11 September 2020

Engineering Escherichia coli NfsB To Activate a Hypoxia-Resistant Analogue of the PET Probe EF5 To Enable Non-Invasive Imaging during Enzyme Prodrug Therapy

scientific article published on 20 August 2019

Engineering a Multifunctional Nitroreductase for Improved Activation of Prodrugs and PET Probes for Cancer Gene Therapy.

scientific article published on 20 February 2017

Engineering the <i>Escherichia coli</i> Nitroreductase NfsA to Create a Flexible Enzyme-Prodrug Activation System

scientific article published on 07 June 2021

Enzyme improvement in the absence of structural knowledge: a novel statistical approach.

scientific article

Error-prone PCR and effective generation of gene variant libraries for directed evolution

scientific article

Escherichia coli NemA is an efficient chromate reductase that can be biologically immobilized to provide a cell free system for remediation of hexavalent chromium.

scientific article

Evaluating the abilities of diverse nitroaromatic prodrug metabolites to exit a model Gram negative vector for bacterial-directed enzyme-prodrug therapy

scientific article published on 21 October 2018

Evaluation of NfsA-like nitroreductases from Neisseria meningitidis and Bartonella henselae for enzyme-prodrug therapy, targeted cellular ablation, and dinitrotoluene bioremediation.

scientific article published on 17 November 2017

Generating Functional Recombinant NRPS Enzymes in the Laboratory Setting via Peptidyl Carrier Protein Engineering

scientific article published on 27 October 2016

Genetic manipulation of non-ribosomal peptide synthetases to generate novel bioactive peptide products

scientific article published on 12 September 2014

Grant lottery systems: a winner responds

scientific article published on 01 March 2020

High-Throughput Screening for Inhibitors of the SARS-CoV-2 Protease Using a FRET-Biosensor

scientific article published on 13 October 2020

Inhibition of Indigoidine Synthesis as a High-Throughput Colourimetric Screen for Antibiotics Targeting the Essential Phosphopantetheinyl Transferase PptT

scientific article published on 12 July 2021

Integrated activities of two alternative sigma factors coordinate iron acquisition and uptake by Pseudomonas aeruginosa

scientific article

Interactions between an anti-sigma protein and two sigma factors that regulate the pyoverdine signaling pathway in Pseudomonas aeruginosa

scientific article

Interrogation of the Structure–Activity Relationship of a Lipophilic Nitroaromatic Prodrug Series Designed for Cancer Gene Therapy Applications

scientific article published in February 2022

Intracellular complexities of acquiring a new enzymatic function revealed by mass-randomisation of active site residues

scientific article published on 13 November 2020

Lamellarin Sulfates from the Pacific Tunicate Didemnum ternerratum

scientific article published on 15 July 2019

Mechanism of Two-/Four-Electron Reduction of Nitroaromatics by Oxygen-Insensitive Nitroreductases: The Role of a Non-Enzymatic Reduction Step

scientific article published on 09 July 2018

Mechanism of chromate reduction by the Escherichia coli protein, NfsA, and the role of different chromate reductases in minimizing oxidative stress during chromate reduction.

scientific article published in August 2004

Mechanistic Understanding Enables the Rational Design of Salicylanilide Combination Therapies for Gram-Negative Infections

scientific article published on 15 September 2020

Mechanistic understanding enables the rational design of salicylanilide combination therapies for Gram-negative infections

scientific article published on 25 April 2020

Metagenome Driven Discovery of Nonribosomal Peptides

scientific article published on 20 September 2019

Metagenomic Exploration of the Marine Sponge Mycale hentscheli Uncovers Multiple Polyketide-Producing Bacterial Symbionts

scientific article published on 24 March 2020

Metathramycin, a new bioactive aureolic acid discovered by heterologous expression of a metagenome derived biosynthetic pathway ⬇️

scientific article published on 2 February 2021

Methods for competitive enrichment and evaluation of superior DNA ligases

scientific article published on 20 May 2020

New enzyme for reductive cancer chemotherapy, YieF, and its improvement by directed evolution

scientific article published in January 2006

Nitroreductase gene-directed enzyme prodrug therapy: insights and advances toward clinical utility

scientific article published on October 2015

Portability of the thiolation domain in recombinant pyoverdine non-ribosomal peptide synthetases.

scientific article published on 13 August 2015

Protocol for evaluating the abilities of diverse nitroaromatic prodrug metabolites to exit a model Gram negative bacterial vector

scientific article published on 23 January 2020

Pseudomonas aeruginosa MdaB and WrbA are water-soluble two-electron quinone oxidoreductases with the potential to defend against oxidative stress

scientific journal article

Pseudomonas aeruginosa NfsB and nitro-CBI-DEI--a promising enzyme/prodrug combination for gene directed enzyme prodrug therapy

scientific article published on 10 June 2013

Rapid and flexible biochemical assays for evaluating 4'-phosphopantetheinyl transferase activity.

scientific article

Rational design of an AKR1C3-resistant analog of PR-104 for enzyme-prodrug therapy

scientific article

Reduction of quinones and nitroaromatic compounds by Escherichia coli nitroreductase A (NfsA): Characterization of kinetics and substrate specificity

scientific article published on 13 December 2016

Restoring Tumour Selectivity of the Bioreductive Prodrug PR-104 by Developing an Analogue Resistant to Aerobic Metabolism by Human Aldo-Keto Reductase 1C3

scientific article published on 26 November 2021

Secondary metabolism in the lichen symbiosis

scientific article published on 2 November 2017

Site-saturation mutagenesis by overlap extension PCR.

scientific article

Skyllamycins D and E, Non-Ribosomal Cyclic Depsipeptides from Lichen-Sourced Streptomyces anulatus

scientific article published on 7 September 2021

Structural Evaluation of a Nitroreductase Engineered for Improved Activation of the 5-Nitroimidazole PET Probe SN33623

scientific article published on 15 June 2024

Structural, functional and evolutionary perspectives on effective re-engineering of non-ribosomal peptide synthetase assembly lines

scientific article published on 01 November 2018

Substrate specificity of the nonribosomal peptide synthetase PvdD from Pseudomonas aeruginosa

scientific journal article

Suggesting reviewers affects outcome?

scientific article

The Flavin Reductase MsuE Is a Novel Nitroreductase that Can Efficiently Activate Two Promising Next-Generation Prodrugs for Gene-Directed Enzyme Prodrug Therapy

scientific article

The indigoidine synthetase BpsA provides a colorimetric ATP assay that can be adapted to quantify the substrate preferences of other NRPS enzymes

scientific article published on 17 July 2020

Total Synthesis and Bioactivity Studies of Fungal Metabolite (-)-TAN-2483B

scientific article published on 24 November 2020

Toward a high-throughput screening platform for directed evolution of enzymes that activate genotoxic prodrugs.

scientific article

Understanding biosynthetic protein-protein interactions

scientific article published on 01 November 2018

Use of an optimised enzyme/prodrug combination for Clostridia directed enzyme prodrug therapy induces a significant growth delay in necrotic tumours

scientific article published on 8 February 2021

uvrB gene deletion enhances SOS chromotest sensitivity for nitroreductases that preferentially generate the 4-hydroxylamine metabolite of the anti-cancer prodrug CB1954.

scientific article published on 19 August 2010

List of works by David F. Ackerley

A cofactor consumption screen identifies promising NfsB family nitroreductases for dinitrotoluene remediation

A functional screen for recovery of 4'-phosphopantetheinyl transferase and associated natural product biosynthesis genes from metagenome libraries.

A gain-of-function positive-selection expression plasmid that enables high-efficiency cloning

A giant leap in sequence space reveals the intracellular complexities of evolving a new function

A metagenomic library cloning strategy that promotes high-level expression of captured genes to enable efficient functional screening

A sensitive single-enzyme assay system using the non-ribosomal peptide synthetase BpsA for measurement of L-glutamine in biological samples

Advancing Clostridia to Clinical Trial: Past Lessons and Recent Progress

Bioactivity-Guided Metabolite Profiling of Feijoa ( Acca sellowiana) Cultivars Identifying 4-Cyclopentene-1,3-dione as a Potent Antifungal Inhibitor of Chitin Synthesis.

Biosynthesis of novel Pyoverdines by domain substitution in a nonribosomal peptide synthetase of Pseudomonas aeruginosa

Characterization and genetic manipulation of peptide synthetases in Pseudomonas aeruginosa PAO1 in order to generate novel pyoverdines

Characterization of pyoverdine and achromobactin in Pseudomonas syringae pv. phaseolicola 1448a

ChrR, a soluble quinone reductase of Pseudomonas putida that defends against H2O2.

Chromate-reducing properties of soluble flavoproteins from Pseudomonas putida and Escherichia coli.

Correction to Lamellarin Sulfates from the Pacific Tunicate Didemnum ternerratum

Cracking the Nonribosomal Code

Creation and screening of a multi-family bacterial oxidoreductase library to discover novel nitroreductases that efficiently activate the bioreductive prodrugs CB1954 and PR-104A.

Crystal structure of the essential Mycobacterium tuberculosis phosphopantetheinyl transferase PptT, solved as a fusion protein with maltose binding protein

Development of a Mycobacterium smegmatis transposon mutant array for characterising the mechanism of action of tuberculosis drugs: Findings with isoniazid and its structural analogues

Directed Evolution of the Nonribosomal Peptide Synthetase BpsA to Enable Recognition by the Human Phosphopantetheinyl Transferases for Counter-Screening Antibiotic Candidates

Directed evolution of the B. subtilis nitroreductase YfkO improves activation of the PET-capable probe SN33623 and CB1954 prodrug

Discovery and evaluation of Escherichia coli nitroreductases that activate the anti-cancer prodrug CB1954.

Effect of chromate stress on Escherichia coli K-12.

Efficient rational modification of non-ribosomal peptides by adenylation domain substitution

Engineering Escherichia coli NfsB To Activate a Hypoxia-Resistant Analogue of the PET Probe EF5 To Enable Non-Invasive Imaging during Enzyme Prodrug Therapy

Engineering a Multifunctional Nitroreductase for Improved Activation of Prodrugs and PET Probes for Cancer Gene Therapy.

Engineering the <i>Escherichia coli</i> Nitroreductase NfsA to Create a Flexible Enzyme-Prodrug Activation System

Enzyme improvement in the absence of structural knowledge: a novel statistical approach.

Error-prone PCR and effective generation of gene variant libraries for directed evolution

Escherichia coli NemA is an efficient chromate reductase that can be biologically immobilized to provide a cell free system for remediation of hexavalent chromium.

Evaluating the abilities of diverse nitroaromatic prodrug metabolites to exit a model Gram negative vector for bacterial-directed enzyme-prodrug therapy

Evaluation of NfsA-like nitroreductases from Neisseria meningitidis and Bartonella henselae for enzyme-prodrug therapy, targeted cellular ablation, and dinitrotoluene bioremediation.

Generating Functional Recombinant NRPS Enzymes in the Laboratory Setting via Peptidyl Carrier Protein Engineering

Genetic manipulation of non-ribosomal peptide synthetases to generate novel bioactive peptide products

Grant lottery systems: a winner responds

High-Throughput Screening for Inhibitors of the SARS-CoV-2 Protease Using a FRET-Biosensor

Inhibition of Indigoidine Synthesis as a High-Throughput Colourimetric Screen for Antibiotics Targeting the Essential Phosphopantetheinyl Transferase PptT

Integrated activities of two alternative sigma factors coordinate iron acquisition and uptake by Pseudomonas aeruginosa

Interactions between an anti-sigma protein and two sigma factors that regulate the pyoverdine signaling pathway in Pseudomonas aeruginosa

Interrogation of the Structure–Activity Relationship of a Lipophilic Nitroaromatic Prodrug Series Designed for Cancer Gene Therapy Applications

Intracellular complexities of acquiring a new enzymatic function revealed by mass-randomisation of active site residues

Lamellarin Sulfates from the Pacific Tunicate Didemnum ternerratum

Mechanism of Two-/Four-Electron Reduction of Nitroaromatics by Oxygen-Insensitive Nitroreductases: The Role of a Non-Enzymatic Reduction Step

Mechanism of chromate reduction by the Escherichia coli protein, NfsA, and the role of different chromate reductases in minimizing oxidative stress during chromate reduction.

Mechanistic Understanding Enables the Rational Design of Salicylanilide Combination Therapies for Gram-Negative Infections

Mechanistic understanding enables the rational design of salicylanilide combination therapies for Gram-negative infections

Metagenome Driven Discovery of Nonribosomal Peptides

Metagenomic Exploration of the Marine Sponge Mycale hentscheli Uncovers Multiple Polyketide-Producing Bacterial Symbionts

Metathramycin, a new bioactive aureolic acid discovered by heterologous expression of a metagenome derived biosynthetic pathway ⬇️

Methods for competitive enrichment and evaluation of superior DNA ligases

New enzyme for reductive cancer chemotherapy, YieF, and its improvement by directed evolution

Nitroreductase gene-directed enzyme prodrug therapy: insights and advances toward clinical utility

Portability of the thiolation domain in recombinant pyoverdine non-ribosomal peptide synthetases.

Protocol for evaluating the abilities of diverse nitroaromatic prodrug metabolites to exit a model Gram negative bacterial vector

Pseudomonas aeruginosa MdaB and WrbA are water-soluble two-electron quinone oxidoreductases with the potential to defend against oxidative stress

Pseudomonas aeruginosa NfsB and nitro-CBI-DEI--a promising enzyme/prodrug combination for gene directed enzyme prodrug therapy

Rapid and flexible biochemical assays for evaluating 4'-phosphopantetheinyl transferase activity.

Rational design of an AKR1C3-resistant analog of PR-104 for enzyme-prodrug therapy

Reduction of quinones and nitroaromatic compounds by Escherichia coli nitroreductase A (NfsA): Characterization of kinetics and substrate specificity

Restoring Tumour Selectivity of the Bioreductive Prodrug PR-104 by Developing an Analogue Resistant to Aerobic Metabolism by Human Aldo-Keto Reductase 1C3

Secondary metabolism in the lichen symbiosis

Site-saturation mutagenesis by overlap extension PCR.

Skyllamycins D and E, Non-Ribosomal Cyclic Depsipeptides from Lichen-Sourced Streptomyces anulatus

Structural Evaluation of a Nitroreductase Engineered for Improved Activation of the 5-Nitroimidazole PET Probe SN33623

Structural, functional and evolutionary perspectives on effective re-engineering of non-ribosomal peptide synthetase assembly lines

Substrate specificity of the nonribosomal peptide synthetase PvdD from Pseudomonas aeruginosa

Suggesting reviewers affects outcome?

The Flavin Reductase MsuE Is a Novel Nitroreductase that Can Efficiently Activate Two Promising Next-Generation Prodrugs for Gene-Directed Enzyme Prodrug Therapy

The indigoidine synthetase BpsA provides a colorimetric ATP assay that can be adapted to quantify the substrate preferences of other NRPS enzymes

Total Synthesis and Bioactivity Studies of Fungal Metabolite (-)-TAN-2483B

Toward a high-throughput screening platform for directed evolution of enzymes that activate genotoxic prodrugs.

Understanding biosynthetic protein-protein interactions

Use of an optimised enzyme/prodrug combination for Clostridia directed enzyme prodrug therapy induces a significant growth delay in necrotic tumours

uvrB gene deletion enhances SOS chromotest sensitivity for nitroreductases that preferentially generate the 4-hydroxylamine metabolite of the anti-cancer prodrug CB1954.