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List of works by Bernd Nidetzky

A Parsimonious Mechanism of Sugar Dehydration by Human GDP-Mannose-4,6-dehydratase

scientific article published on 01 March 2019

A Spring in Performance: Silica Nanosprings Boost Enzyme Immobilization in Microfluidic Channels

scientific article

A catalytic consensus motif for D-mannitol 2-dehydrogenase, a member of a polyol-specific long-chain dehydrogenase family, revealed by kinetic characterization of site-directed mutants of the enzyme from Pseudomonas fluorescens

scientific article published on October 2002

A tailor-made, self-sufficient and recyclable monooxygenase catalyst based on coimmobilized cytochrome P450 BM3 and glucose dehydrogenase

scientific article published on 29 August 2018

Active-Site His85 of Pasteurella dagmatis Sialyltransferase Facilitates Productive Sialyl Transfer and So Prevents Futile Hydrolysis of CMP-Neu5Ac.

scientific article

Adsorption and desorption of self-assembled L-cysteine monolayers on nanoporous gold monitored by in situ resistometry

scientific article published on 18 November 2019

Altering the coenzyme preference of xylose reductase to favor utilization of NADH enhances ethanol yield from xylose in a metabolically engineered strain of Saccharomyces cerevisiae

scientific article

An ortho C-methylation/O-glycosylation motif on a hydroxy-coumarin scaffold, selectively installed by biocatalysis

scientific article

Analysis and prediction of the physiological effects of altered coenzyme specificity in xylose reductase and xylitol dehydrogenase during xylose fermentation by Saccharomyces cerevisiae ⬇️

scientific article published on August 25, 2011

Binding pattern of intermediate UDP-4-keto-xylose to human UDP-xylose synthase: Synthesis and STD NMR of model keto-saccharides

scientific article published on 5 November 2016

Biobased, Internally pH-Sensitive Materials: Immobilized Yellow Fluorescent Protein as an Optical Sensor for Spatiotemporal Mapping of pH Inside Porous Matrices.

scientific article published on 31 January 2018

Biochemical characterization and mechanistic analysis of the levoglucosan kinase from Lipomyces starkeyi.

scientific article published on 18 December 2017

Biochemical characterization and mutational analysis of the mononuclear non-haem Fe2+ site in Dke1, a cupin-type dioxygenase from Acinetobacter johnsonii

scientific article

Biotechnological production of fucosylated human milk oligosaccharides: Prokaryotic fucosyltransferases and their use in biocatalytic cascades or whole cell conversion systems

scientific article published on April 2016

Biotransformations in microstructured reactors: more than flowing with the stream? ⬇️

scientific article published on 04 May 2011

Carbohydrate synthesis by disaccharide phosphorylases: Reactions, catalytic mechanisms and application in the glycosciences ⬇️

scientific article published on December 1, 2010

Carrier-free immobilized enzymes for biocatalysis

scientific article

Catalytic mechanism and substrate selectivity of aldo-keto reductases: insights from structure-function studies of Candida tenuis xylose reductase

scientific article published on September 2006

Catalytic mechanism of Zn2+-dependent polyol dehydrogenases: kinetic comparison of sheep liver sorbitol dehydrogenase with wild-type and Glu154-->Cys forms of yeast xylitol dehydrogenase

scientific article

Catalytic mechanism of alpha-retaining glucosyl transfer by Corynebacterium callunae starch phosphorylase: the role of histidine-334 examined through kinetic characterization of site-directed mutants

scientific article published on April 2005

Catalytic mechanism of human UDP-glucose 6-dehydrogenase: in situ proton NMR studies reveal that the C-5 hydrogen of UDP-glucose is not exchanged with bulk water during the enzymatic reaction ⬇️

scientific article published on April 2, 2012

Catalytic reaction profile for NADH-dependent reduction of aromatic aldehydes by xylose reductase from Candida tenuis

scientific article published on September 2002

Cellular automata modeling depicts degradation of cellulosic material by a cellulase system with single-molecule resolution

scientific article

Cellulases dig deep: in situ observation of the mesoscopic structural dynamics of enzymatic cellulose degradation

scientific article

Cellulose surface degradation by a lytic polysaccharide monooxygenase and its effect on cellulase hydrolytic efficiency

scientific article

Characterization of a laboratory-scale container for freezing protein solutions with detailed evaluation of a freezing process simulation

scientific article published on 13 December 2013

Chiral resolution through stereoselective transglycosylation by sucrose phosphorylase: application to the synthesis of a new biomimetic compatible solute, (R)-2-O-α-D-glucopyranosyl glyceric acid amide

scientific article published on January 2014

Combining expression and process engineering for high-quality production of human sialyltransferase in Pichia pastoris

scientific article published on 24 March 2016

Complete switch from α-2,3- to α-2,6-regioselectivity in Pasteurella dagmatis β-D-galactoside sialyltransferase by active-site redesign

scientific article

Conformational flexibility related to enzyme activity: evidence for a dynamic active-site gatekeeper function of Tyr(215) in Aerococcus viridans lactate oxidase

scientific article

Controllable Iterative β-Glucosylation from UDP-Glucose by <i>Bacillus cereus</i> Glycosyltransferase GT1: Application for the Synthesis of Disaccharide-Modified Xenobiotics

scientific article published on 24 November 2021

Convenient microtiter plate-based, oxygen-independent activity assays for flavin-dependent oxidoreductases based on different redox dyes.

scientific article

CorNet: Assigning function to networks of co-evolving residues by automated literature mining

scientific article

Crystal structure of Pseudomonas fluorescens mannitol 2-dehydrogenase binary and ternary complexes. Specificity and catalytic mechanism

scientific article

Crystallographic snapshots of UDP-glucuronic acid 4-epimeraseligand binding, rotation and reduction

scientific article published on 13 July 2020

Deciphering the enzymatic mechanism of sugar ring contraction in UDP-apiose biosynthesis

scientific article published on 01 November 2019

Decoupling of recombinant protein production from Escherichia coli cell growth enhances functional expression of plant Leloir glycosyltransferases

scientific article published on 05 February 2019

Design of the Enzyme-Carrier Interface to Overcome the O<sub>2</sub> and NADH Mass Transfer Limitations of an Immobilized Flavin Oxidase

scientific article published on 04 December 2020

Development of an ultrahigh-temperature process for the enzymatic hydrolysis of lactose. IV. Immobilization of two thermostable beta-glycosidases and optimization of a packed-bed reactor for lactose conversion

scientific article

Diastereoselective Synthesis of Glycosyl Phosphates by Using a Phosphorylase-Phosphatase Combination Catalyst

scientific article published on 13 November 2015

Direct-Write Fabrication of Cellulose Nano-Structures via Focused Electron Beam Induced Nanosynthesis

scientific article

Dual-lifetime referencing (DLR): a powerful method for on-line measurement of internal pH in carrier-bound immobilized biocatalysts.

scientific article

Dynamic mechanism of proton transfer in mannitol 2-dehydrogenase from Pseudomonas fluorescens: mobile GLU292 controls proton relay through a water channel that connects the active site with bulk solvent

scientific article

Encapsulation of Trigonopsis variabilis D-amino acid oxidase and fast comparison of the operational stabilities of free and immobilized preparations of the enzyme

scientific article published on 01 February 2008

Engineering Candida tenuis Xylose reductase for improved utilization of NADH: antagonistic effects of multiple side chain replacements and performance of site-directed mutants under simulated in vivo conditions

scientific article published on October 2005

Enzymatic Redox Cascade for One-Pot Synthesis of Uridine 5'-Diphosphate Xylose from Uridine 5'-Diphosphate Glucose

scientific article published on 5 November 2014

Evidence of a sequestered imine intermediate during reduction of nitrile to amine by the nitrile reductase QueF from Escherichia coli

scientific article

Expanding the Enzyme Repertoire for Sugar Nucleotide Epimerization: The CDP-Tyvelose 2-Epimerase from <i>Thermodesulfatator atlanticus</i> for Glucose/Mannose Interconversion

scientific article published on 04 December 2020

Fermentation of mixed glucose-xylose substrates by engineered strains of Saccharomyces cerevisiae: role of the coenzyme specificity of xylose reductase, and effect of glucose on xylose utilization

scientific article

From wheat straw to bioethanol: integrative analysis of a separate hydrolysis and co-fermentation process with implemented enzyme production

scientific article

Functional characterization of an orphan cupin protein from Burkholderia xenovorans reveals a mononuclear nonheme Fe2+-dependent oxygenase that cleaves beta-diketones

scientific article

Functional characterization of the native swollenin from Trichoderma reesei: study of its possible role as C1 factor of enzymatic lignocellulose conversion

scientific article published on 26 August 2016

Fusion to a pull-down domain: a novel approach of producing Trigonopsis variabilisD-amino acid oxidase as insoluble enzyme aggregates

scientific article published in June 2007

Glucosylglycerol and glucosylglycerate as enzyme stabilizers.

scientific article

Glycosides as compatible solutes: biosynthesis and applications ⬇️

scientific article published on March 10, 2011

Glycosyltransferase cascades made fit for chemical production: Integrated biocatalytic process for the natural polyphenol C-glucoside nothofagin

scientific article published on 13 November 2017

Glycosynthase reaction meets the flow: Continuous synthesis of lacto-N-triose II by engineered β-hexosaminidase immobilized on solid support

scientific article published on 03 February 2020

High-level expression of Rhodotorula gracilis d-amino acid oxidase in Pichia pastoris

article

Host cell and expression engineering for development of an E. coli ketoreductase catalyst: enhancement of formate dehydrogenase activity for regeneration of NADH.

scientific article

Human Enzymes for Organic Synthesis

scientific article published on 11 September 2018

Hydrolysis of lactose by beta-glycosidase CelB from hyperthermophilic archaeon Pyrococcus furiosus: comparison of hollow-fiber membrane and packed-bed immobilized enzyme reactors for continuous processing of ultrahigh temperature-treated skim milk

scientific article

Identification of novel metabolic interactions controlling carbon flux from xylose to ethanol in natural and recombinant yeasts

scientific article

In situ protein secondary structure determination in ice: Raman spectroscopy-based process analytical tool for frozen storage of biopharmaceuticals.

scientific article

Interplay of catalytic subsite residues in the positioning of α-d-glucose 1-phosphate in sucrose phosphorylase

scientific article published on 17 April 2015

Interplay of nucleophilic catalysis with proton transfer in the nitrile reductase QueF from <i>Escherichia coli</i>

scientific article published in 2019

Isotope Probing of the UDP-Apiose/UDP-Xylose Synthase Reaction: Evidence of a Mechanism via a Coupled Oxidation and Aldol Cleavage

scientific article published on 19 January 2017

Kinetic and CD/MCD spectroscopic studies of the atypical, three-His-ligated, non-heme Fe2+ center in diketone dioxygenase: the role of hydrophilic outer shell residues in catalysis

scientific article published on February 2010

L-Lactic acid production from glucose and xylose with engineered strains of Saccharomyces cerevisiae: aeration and carbon source influence yields and productivities.

scientific article published on 11 April 2018

Lacto-N-tetraose synthesis by wild-type and glycosynthase variants of the β-N-hexosaminidase from Bifidobacterium bifidum

scientific article published on 01 June 2019

Leloir Glycosyltransferases and Natural Product Glycosylation: Biocatalytic Synthesis of the C-Glucoside Nothofagin, a Major Antioxidant of Redbush Herbal Tea.

scientific article published on 20 August 2013

Let the substrate flow, not the enzyme: Practical immobilization of d-amino acid oxidase in a glass microreactor for effective biocatalytic conversions

scientific article

Limitations in Xylose-Fermenting Saccharomyces cerevisiae, Made Evident through Comprehensive Metabolite Profiling and Thermodynamic Analysis ⬇️

scientific article published on October 1, 2010

Magnetically responsive horseradish peroxidase@ZIF-8 for biocatalysis

scientific article published on 23 April 2020

Mannitol metabolism in brown algae involves a new phosphatase family

scientific article

Mechanistic characterization of UDP-glucuronic acid 4-epimerase

scientific article published on 09 July 2020

Mechanistic differences among retaining disaccharide phosphorylases: insights from kinetic analysis of active site mutants of sucrose phosphorylase and alpha,alpha-trehalose phosphorylase

scientific article published on 02 February 2008

Mechanistic study of CMP-Neu5Ac hydrolysis by α2,3-sialyltransferase from Pasteurella dagmatis

scientific article

Modeling the activity burst in the initial phase of cellulose hydrolysis by the processive cellobiohydrolase Cel7A

scientific article published on 08 January 2019

Multiple forms of xylose reductase in Candida intermedia: comparison of their functional properties using quantitative structure-activity relationships, steady-state kinetic analysis, and pH studies

scientific article

Mutagenesis of the dimer interface region of Corynebacterium callunae starch phosphorylase perturbs the phosphate-dependent conformational relay that enhances oligomeric stability of the enzyme.

scientific article

Non-native aggregation of recombinant human granulocyte-colony stimulating factor under simulated process stress conditions

scientific article

On the donor substrate dependence of group transfer reactions by hydrolytic enzymes: insight from kinetic analysis of sucrose phosphorylase-catalyzed transglycosylation

scientific article published on 23 June 2020

Oriented Coimmobilization of Oxidase and Catalase on Tailor-Made Ordered Mesoporous Silica

scientific article published on 2 May 2017

Orthophosphate binding at the dimer interface of Corynebacterium callunae starch phosphorylase: mutational analysis of its role for activity and stability of the enzyme

scientific article

Oxidation of Monolignols by Members of the Berberine Bridge Enzyme Family Suggests a Role in Plant Cell Wall Metabolism

scientific article published on 2 June 2015

Plasmid Design for Tunable Two-Enzyme Co-Expression Promotes Whole-Cell Production of Cellobiose

scientific article published on 15 July 2020

Probing of the reaction pathway of human UDP-xylose synthase with site-directed mutagenesis

scientific article published on 17 August 2015

Probing the active site of Corynebacterium callunae starch phosphorylase through the characterization of wild-type and His334-->Gly mutant enzymes

scientific article published on 04 September 2007

Process intensification for O2 -dependent enzymatic transformations in continuous single-phase pressurized flow

scientific article published on 08 January 2019

Process intensification for cytochrome P450 BM3-catalyzed oxy-functionalization of dodecanoic acid

scientific article published on 05 May 2020

Process intensification through microbial strain evolution: mixed glucose-xylose fermentation in wheat straw hydrolyzates by three generations of recombinant Saccharomyces cerevisiae

scientific article

Product solubility control in cellooligosaccharide production by coupled cellobiose and cellodextrin phosphorylase

scientific article published on 21 May 2019

Protein freeze concentration and micro-segregation analysed in a temperature-controlled freeze container

scientific article

Pushing the limits: Cyclodextrin-based intensification of bioreductions

scientific article published on 18 November 2020

Recombinant sucrose phosphorylase from Leuconostoc mesenteroides: characterization, kinetic studies of transglucosylation, and application of immobilised enzyme for production of alpha-D-glucose 1-phosphate

scientific article published on 02 December 2006

Reverse C-glycosidase reaction provides C-nucleotide building blocks of xenobiotic nucleic acids

scientific article published on 08 December 2020

Rules for biocatalyst and reaction engineering to implement effective, NAD(P)H-dependent, whole cell bioreductions

scientific article

Saccharomyces cerevisiae strain comparison in glucose-xylose fermentations on defined substrates and in high-gravity SSCF: convergence in strain performance despite differences in genetic and evolutionary engineering history.

scientific article published on 4 September 2017

Single-site oxidation, cysteine 108 to cysteine sulfinic acid, in D-amino acid oxidase from Trigonopsis variabilis and its structural and functional consequences ⬇️

scientific article

Speeding up the product release: a second-sphere contribution from Tyr191 to the reactivity of L-lactate oxidase revealed in crystallographic and kinetic studies of site-directed variants

scientific article

Stepwise engineering of a Pichia pastoris D-amino acid oxidase whole cell catalyst.

scientific article published on 26 April 2010

Stepwise metabolic adaption from pure metabolization to balanced anaerobic growth on xylose explored for recombinant Saccharomyces cerevisiae

scientific article

Structural and Kinetic Evidence That Catalytic Reaction of Human UDP-glucose 6-Dehydrogenase Involves Covalent Thiohemiacetal and Thioester Enzyme Intermediates

scientific article

Structural and functional comparison of 2-His-1-carboxylate and 3-His metallocentres in non-haem iron(II)-dependent enzymes

scientific article

Structural and kinetic studies of induced fit in xylulose kinase from Escherichia coli

scientific article

Structure and Mechanism of Human UDP-glucose 6-Dehydrogenase

scientific article

Structure and mechanism of human UDP-xylose synthase: evidence for a promoting role of sugar ring distortion in a three-step catalytic conversion of UDP-glucuronic acid

scientific article published on 18 July 2012

Structure based descriptors for the estimation of colloidal interactions and protein aggregation propensities

scientific article

Structure of xylose reductase bound to NAD+ and the basis for single and dual co-substrate specificity in family 2 aldo-keto reductases ⬇️

scientific article (publication date: 15 July 2003)

Structure-function relationships for Schizophyllum commune trehalose phosphorylase and their implications for the catalytic mechanism of family GT-4 glycosyltransferases

scientific article published on August 2006

Substrate-binding recognition and specificity of trehalose phosphorylase from Schizophyllum commune examined in steady-state kinetic studies with deoxy and deoxyfluoro substrate analogues and inhibitors

scientific article

Sucrose synthase: A unique glycosyltransferase for biocatalytic glycosylation process development

scientific article

The Ala95-to-Gly substitution in Aerococcus viridans l-lactate oxidase revisited - structural consequences at the catalytic site and effect on reactivity with O2 and other electron acceptors

scientific article

The Microenvironment in Immobilized Enzymes: Methods of Characterization and Its Role in Determining Enzyme Performance

scientific article published on 24 September 2019

The coenzyme specificity of Candida tenuis xylose reductase (AKR2B5) explored by site-directed mutagenesis and X-ray crystallography

scientific article published on January 2005

The influence of feedstock characteristics on enzyme production in Trichoderma reesei: a review on productivity, gene regulation and secretion profiles

scientific article published on 08 October 2019

The stabilizing effects of immobilization in D-amino acid oxidase from Trigonopsis variabilis

scientific article

The structure of apo and holo forms of xylose reductase, a dimeric aldo-keto reductase from Candida tenuis

scientific article

Three-Enzyme Phosphorylase Cascade for Integrated Production of Short-Chain Cellodextrins

scientific article published on 14 November 2019

Tracking interactions that stabilize the dimer structure of starch phosphorylase from Corynebacterium callunae. Roles of Arg234 and Arg242 revealed by sequence analysis and site-directed mutagenesis

scientific article

Trehalose phosphorylase from Pleurotus ostreatus: characterization and stabilization by covalent modification, and application for the synthesis of alpha,alpha-trehalose

scientific article published on 02 December 2006

Trigonopsis variabilis D-amino acid oxidase: control of protein quality and opportunities for biocatalysis through production in Escherichia coli

scientific article published on 20 October 2006

Tunable Semicrystalline Thin Film Cellulose Substrate for High-Resolution, In-Situ AFM Characterization of Enzymatic Cellulose Degradation

scientific article published on 30 November 2015

UDP-glucose dehydrogenase: structure and function of a potential drug target

scientific article published on October 2010

Unexpected NADPH Hydratase Activity in the Nitrile Reductase QueF from Escherichia coli

scientific article published on 18 December 2019

Visualizing cellulase activity

scientific article published on 26 March 2013

Walking a Fine Line with Sucrose Phosphorylase: Efficient Single-Step Biocatalytic Production of l-Ascorbic Acid 2-Glucoside from Sucrose

scientific article published on 20 April 2017

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