List of works - Colin Scott

300-Fold Increase in Production of the Zn2+-Dependent Dechlorinase TrzN in Soluble Form via Apoenzyme Stabilization

scientific article

A 5000-Fold Increase in the Specificity of a Bacterial Phosphotriesterase for Malathion through Combinatorial Active Site Mutagenesis

scientific article

A free-enzyme catalyst for the bioremediation of environmental atrazine contamination

scientific article published on 31 May 2010

A global response to sulfur starvation inPseudomonas putidaand its relationship to the expression of low-sulfur-content proteins

scientific article published on 20 December 2006

A member of the cAMP receptor protein family of transcription regulators in Mycobacterium tuberculosis is required for virulence in mice and controls transcription of the rpfA gene coding for a resuscitation promoting factor

scientific journal article

A member of the cAMP receptor protein family of transcription regulators in Mycobacterium tuberculosis is required for virulence in mice and controls transcription of the rpfA gene coding for a resuscitation promoting factor

article

A novel decarboxylating amidohydrolase involved in avoiding metabolic dead ends during cyanuric acid catabolism in Pseudomonas sp. strain ADP

scientific article published in PLoS ONE

A revised biosynthetic pathway for the cofactor F in prokaryotes

scientific article published on 05 April 2019

A β-Alanine Catabolism Pathway Containing a Highly Promiscuous ω-Transaminase in the 12-Aminododecanate-Degrading Pseudomonas sp. Strain AAC.

scientific article published on 22 April 2016

Active Site Desolvation and Thermostability Trade-Offs in the Evolution of Catalytically Diverse Triazine Hydrolases

scientific article published on 21 October 2016

Adaptive laboratory evolution of native methanol assimilation in Saccharomyces cerevisiae

scientific article published on 04 November 2020

An improved and general streamlined phylogenetic protocol applied to the fatty acid desaturase family

scientific article

An in vivo gene amplification system for high level expression in Saccharomyces cerevisiae

scientific article published on 24 May 2022

An unexpected vestigial protein complex reveals the evolutionary origins of an s-triazine catabolic enzyme.

scientific article published on 9 March 2018

Bacterial degradation of strobilurin fungicides: a role for a promiscuous methyl esterase activity of the subtilisin proteases?

article published in 2011

Catalytic improvement and evolution of atrazine chlorohydrolase

scientific article

Characterisation of Hybrid Polymersome Vesicles Containing the Efflux Pumps NaAtm1 or P-Glycoprotein

scientific article published on 03 May 2020

Characterization of novel mevalonate kinases from the tardigrade <i>Ramazzottius varieornatus</i> and the psychrophilic archaeon <i>Methanococcoides burtonii</i>

scientific article published in 2024

Characterization of the Escherichia coli K-12 ydhYVWXUT operon: regulation by FNR, NarL and NarP.

scientific article published on February 2008

Characterization of the Lactococcus lactis transcription factor FlpA and demonstration of an in vitro switch

scientific article published on 01 March 2000

Characterization of the phenylurea hydrolases A and B: founding members of a novel amidohydrolase subgroup

scientific article

Cofactor promiscuity among F420-dependent reductases enables them to catalyse both oxidation and reduction of the same substrate

Computer-Guided Surface Engineering for Enzyme Improvement

scientific article published in Scientific Reports

Constrained evolution of a bispecific enzyme: lessons for biocatalyst design

scientific article published on 5 January 2017

Crystal structure of a putrescine aminotransferase from Pseudomonas sp. strain AAC.

scientific article published on January 2017

Crystallization and preliminary X-ray diffraction analysis of the amidase domain of allophanate hydrolase from Pseudomonas sp. strain ADP.

scientific article published on 19 February 2014

Cyanuric acid hydrolase: evolutionary innovation by structural concatenation

scientific article published in June 2013

DNA target sequence and FNR-dependent gene expression

scientific article published in April 2003

Determination of the Structure of the Catabolic N-Succinylornithine Transaminase (AstC) from Escherichia coli

scientific article

Efficacy of an organophosphorus hydrolase enzyme (OpdA) in human serum and minipig models of organophosphorus insecticide poisoning

scientific article published on 27 August 2019

Escherichia coli transcriptome dynamics during the transition from anaerobic to aerobic conditions

scientific article published on 20 July 2006

Esterase-based metabolic resistance to insecticides in heliothine and spodopteran pests

scholarly article by Claire A. Farnsworth et al published 2010 in Journal of Pesticide Science

Evolution of atrazine-degrading capabilities in the environment.

scientific article published on 18 October 2012

Evolutionary expansion of the amidohydrolase superfamily in bacteria in response to the synthetic compounds molinate and diuron

scientific article

F420-dependent enzymes - potential for applications in biotechnology

scientific article published on 23 October 2012

F420H2-dependent degradation of aflatoxin and other furanocoumarins is widespread throughout the actinomycetales

scientific article

Functional versatility in the CRP-FNR superfamily of transcription factors: FNR and FLP.

scientific article published in January 2001

Gene identification and proteomic analysis of the esterases of the cotton bollworm, Helicoverpa armigera

scientific article

Heterologous expression and biochemical characterisation of fourteen esterases from Helicoverpa armigera

scientific article (publication date: 2013)

Heterologous expression of the methyl carbamate-degrading hydrolase MCD.

scientific article published on 19 September 2009

High-Resolution X-Ray Structures of Two Functionally Distinct Members of the Cyclic Amide Hydrolase Family of Toblerone Fold Enzymes.

scientific article published on 24 February 2017

How many genetic options for evolving insecticide resistance in heliothine and spodopteran pests?

scientific article published on 20 June 2013

Hyperthermophilic Carbamate Kinase Stability and Anabolic In Vitro Activity at Alkaline pH.

scientific article published on 17 November 2017

Identification and characterization of two families of F420H2-dependent reductases from Mycobacteria that catalyse aflatoxin degradation

scientific article

Identification of novel transaminases from a 12-aminododecanoic acid-metabolizing Pseudomonas strain ⬇️

scientific article published on 24 April 2015

Immobilization of organophosphohydrolase OpdA from Agrobacterium radiobacter by overproduction at the surface of polyester inclusions inside engineered Escherichia coli.

scientific article published on 26 December 2011

Improved production of the non-native cofactor F420 in Escherichia coli

scientific article published in 2021

Intramolecular epistasis and the evolution of a new enzymatic function

scientific article

Isomer-specific comparisons of the hydrolysis of synthetic pyrethroids and their fluorogenic analogues by esterases from the cotton bollworm Helicoverpa armigera.

scientific article published on 16 December 2014

Miscoordination of the iron-sulfur clusters of the anaerobic transcription factor, FNR, allows simple repression but not activation

scientific article published on 09 November 2001

Mycobacterial F420H2-Dependent Reductases Promiscuously Reduce Diverse Compounds through a Common Mechanism.

scientific article

NO sensing by FNR: regulation of the Escherichia coli NO-detoxifying flavohaemoglobin, Hmp.

scientific article published on July 2002

One-Pot Multienzymatic Transformation of NH3, CO2, and Ornithine into the Organic Nitrogen Plant Fertilizer Citrulline Using a Single Recombinant Lysate of E. coli

Ongoing functional evolution of the bacterial atrazine chlorohydrolase AtzA.

scientific article published on 13 April 2013

OpdA, a bacterial organophosphorus hydrolase, prevents lethality in rats after poisoning with highly toxic organophosphorus pesticides

scientific article published on 10 March 2008

Organophosphate and pyrethroid hydrolase activities of mutant Esterases from the cotton bollworm Helicoverpa armigera

scientific article

Over the rainbow: structural characterization of the chromoproteins gfasPurple, amilCP, spisPink and eforRed

scientific article published in 2022

Physiology, Biochemistry, and Applications of F420- and Fo-Dependent Redox Reactions.

scientific article published on 27 April 2016

Proteomic and molecular analyses of esterases associated with monocrotophos resistance in Helicoverpa armigera

scientific article published in 2012

Reverse engineering: transaminase biocatalyst development using ancestral sequence reconstruction

article

Structural and biochemical characterization of the biuret hydrolase (BiuH) from the cyanuric acid catabolism pathway of Rhizobium leguminasorum bv. viciae 3841.

scientific article published on 9 February 2018

Structure-based rational design of a phosphotriesterase

scientific article published on 05 June 2009

Sugar analog synthesis by in vitro biocatalytic cascade: A comparison of alternative enzyme complements for dihydroxyacetone phosphate production as a precursor to rare chiral sugar synthesis

scientific article published on 7 November 2017

The enzymatic basis for pesticide bioremediation.

scientific article

The evolution of new enzyme function: lessons from xenobiotic metabolizing bacteria versus insecticide-resistant insects

scientific article published on March 2011

The evolving story of AtzT, a periplasmic binding protein

scientific article published on 31 October 2019

The structure of the hexameric atrazine chlorohydrolase AtzA

scientific article

Two operons that encode FNR-like proteins in Lactococcus lactis

scientific article published on March 1999

X-Ray Structure and Mutagenesis Studies of the N-Isopropylammelide Isopropylaminohydrolase, AtzC

scientific article

X-ray crystal structure of a malonate-semialdehyde dehydrogenase from Pseudomonas sp. strain AAC.

scientific article published in January 2017

X-ray structure of the amidase domain of AtzF, the allophanate hydrolase from the cyanuric acid-mineralizing multienzyme complex

scientific article

Zinc uptake, oxidative stress and the FNR-like proteins of Lactococcus lactis

scientific article published on 01 November 2000

List of works by Colin Scott

300-Fold Increase in Production of the Zn2+-Dependent Dechlorinase TrzN in Soluble Form via Apoenzyme Stabilization

A 5000-Fold Increase in the Specificity of a Bacterial Phosphotriesterase for Malathion through Combinatorial Active Site Mutagenesis

A free-enzyme catalyst for the bioremediation of environmental atrazine contamination

A global response to sulfur starvation inPseudomonas putidaand its relationship to the expression of low-sulfur-content proteins

A member of the cAMP receptor protein family of transcription regulators in Mycobacterium tuberculosis is required for virulence in mice and controls transcription of the rpfA gene coding for a resuscitation promoting factor

A member of the cAMP receptor protein family of transcription regulators in Mycobacterium tuberculosis is required for virulence in mice and controls transcription of the rpfA gene coding for a resuscitation promoting factor

A novel decarboxylating amidohydrolase involved in avoiding metabolic dead ends during cyanuric acid catabolism in Pseudomonas sp. strain ADP

A revised biosynthetic pathway for the cofactor F in prokaryotes

A β-Alanine Catabolism Pathway Containing a Highly Promiscuous ω-Transaminase in the 12-Aminododecanate-Degrading Pseudomonas sp. Strain AAC.

Active Site Desolvation and Thermostability Trade-Offs in the Evolution of Catalytically Diverse Triazine Hydrolases

Adaptive laboratory evolution of native methanol assimilation in Saccharomyces cerevisiae

An improved and general streamlined phylogenetic protocol applied to the fatty acid desaturase family

An in vivo gene amplification system for high level expression in Saccharomyces cerevisiae

An unexpected vestigial protein complex reveals the evolutionary origins of an s-triazine catabolic enzyme.

Bacterial degradation of strobilurin fungicides: a role for a promiscuous methyl esterase activity of the subtilisin proteases?

Catalytic improvement and evolution of atrazine chlorohydrolase

Characterisation of Hybrid Polymersome Vesicles Containing the Efflux Pumps NaAtm1 or P-Glycoprotein

Characterization of novel mevalonate kinases from the tardigrade <i>Ramazzottius varieornatus</i> and the psychrophilic archaeon <i>Methanococcoides burtonii</i>

Characterization of the Escherichia coli K-12 ydhYVWXUT operon: regulation by FNR, NarL and NarP.

Characterization of the Lactococcus lactis transcription factor FlpA and demonstration of an in vitro switch

Characterization of the phenylurea hydrolases A and B: founding members of a novel amidohydrolase subgroup

Cofactor promiscuity among F420-dependent reductases enables them to catalyse both oxidation and reduction of the same substrate

Computer-Guided Surface Engineering for Enzyme Improvement

Constrained evolution of a bispecific enzyme: lessons for biocatalyst design

Crystal structure of a putrescine aminotransferase from Pseudomonas sp. strain AAC.

Crystallization and preliminary X-ray diffraction analysis of the amidase domain of allophanate hydrolase from Pseudomonas sp. strain ADP.

Cyanuric acid hydrolase: evolutionary innovation by structural concatenation

DNA target sequence and FNR-dependent gene expression

Determination of the Structure of the Catabolic N-Succinylornithine Transaminase (AstC) from Escherichia coli

Efficacy of an organophosphorus hydrolase enzyme (OpdA) in human serum and minipig models of organophosphorus insecticide poisoning

Escherichia coli transcriptome dynamics during the transition from anaerobic to aerobic conditions

Esterase-based metabolic resistance to insecticides in heliothine and spodopteran pests

Evolution of atrazine-degrading capabilities in the environment.

Evolutionary expansion of the amidohydrolase superfamily in bacteria in response to the synthetic compounds molinate and diuron

F420-dependent enzymes - potential for applications in biotechnology

F420H2-dependent degradation of aflatoxin and other furanocoumarins is widespread throughout the actinomycetales

Functional versatility in the CRP-FNR superfamily of transcription factors: FNR and FLP.

Gene identification and proteomic analysis of the esterases of the cotton bollworm, Helicoverpa armigera

Heterologous expression and biochemical characterisation of fourteen esterases from Helicoverpa armigera

Heterologous expression of the methyl carbamate-degrading hydrolase MCD.

High-Resolution X-Ray Structures of Two Functionally Distinct Members of the Cyclic Amide Hydrolase Family of Toblerone Fold Enzymes.

How many genetic options for evolving insecticide resistance in heliothine and spodopteran pests?

Hyperthermophilic Carbamate Kinase Stability and Anabolic In Vitro Activity at Alkaline pH.

Identification and characterization of two families of F420H2-dependent reductases from Mycobacteria that catalyse aflatoxin degradation

Identification of novel transaminases from a 12-aminododecanoic acid-metabolizing Pseudomonas strain ⬇️

Immobilization of organophosphohydrolase OpdA from Agrobacterium radiobacter by overproduction at the surface of polyester inclusions inside engineered Escherichia coli.

Improved production of the non-native cofactor F420 in Escherichia coli

Intramolecular epistasis and the evolution of a new enzymatic function

Isomer-specific comparisons of the hydrolysis of synthetic pyrethroids and their fluorogenic analogues by esterases from the cotton bollworm Helicoverpa armigera.

Miscoordination of the iron-sulfur clusters of the anaerobic transcription factor, FNR, allows simple repression but not activation

Mycobacterial F420H2-Dependent Reductases Promiscuously Reduce Diverse Compounds through a Common Mechanism.

NO sensing by FNR: regulation of the Escherichia coli NO-detoxifying flavohaemoglobin, Hmp.

One-Pot Multienzymatic Transformation of NH3, CO2, and Ornithine into the Organic Nitrogen Plant Fertilizer Citrulline Using a Single Recombinant Lysate of E. coli

Ongoing functional evolution of the bacterial atrazine chlorohydrolase AtzA.

OpdA, a bacterial organophosphorus hydrolase, prevents lethality in rats after poisoning with highly toxic organophosphorus pesticides

Organophosphate and pyrethroid hydrolase activities of mutant Esterases from the cotton bollworm Helicoverpa armigera

Over the rainbow: structural characterization of the chromoproteins gfasPurple, amilCP, spisPink and eforRed

Physiology, Biochemistry, and Applications of F420- and Fo-Dependent Redox Reactions.

Proteomic and molecular analyses of esterases associated with monocrotophos resistance in Helicoverpa armigera

Reverse engineering: transaminase biocatalyst development using ancestral sequence reconstruction

Structural and biochemical characterization of the biuret hydrolase (BiuH) from the cyanuric acid catabolism pathway of Rhizobium leguminasorum bv. viciae 3841.

Structure-based rational design of a phosphotriesterase

Sugar analog synthesis by in vitro biocatalytic cascade: A comparison of alternative enzyme complements for dihydroxyacetone phosphate production as a precursor to rare chiral sugar synthesis

The enzymatic basis for pesticide bioremediation.

The evolution of new enzyme function: lessons from xenobiotic metabolizing bacteria versus insecticide-resistant insects

The evolving story of AtzT, a periplasmic binding protein

The structure of the hexameric atrazine chlorohydrolase AtzA

Two operons that encode FNR-like proteins in Lactococcus lactis

X-Ray Structure and Mutagenesis Studies of the N-Isopropylammelide Isopropylaminohydrolase, AtzC

X-ray crystal structure of a malonate-semialdehyde dehydrogenase from Pseudomonas sp. strain AAC.

X-ray structure of the amidase domain of AtzF, the allophanate hydrolase from the cyanuric acid-mineralizing multienzyme complex

Zinc uptake, oxidative stress and the FNR-like proteins of Lactococcus lactis