List of works - Yasuaki Kawarasaki

A long-lived batch reaction system of cell-free protein synthesis

scientific article published on 01 April 1995

A method for functional mapping of protein-protein binding domain by preferential amplification of the shortest amplicon using PCR.

scientific article

A method for reverse interactome analysis: High-resolution mapping of interdomain interaction network in Dam1 complex and its specific disorganization based on the interaction domain expression

scientific article published on 01 July 2010

A periplasmic fluorescent reporter protein and its application in high-throughput membrane protein topology analysis.

scientific article published in August 2004

A trimmed viral cap-independent translation enhancing sequence for rapid in vitro gene expression

scientific article published on 01 May 2000

Analysing the substrate multispecificity of a proton-coupled oligopeptide transporter using a dipeptide library

scientific article

Analyzing a dipeptide library to identify human dipeptidyl peptidase IV inhibitor.

scientific article published on 28 November 2014

Chimeric gene library construction by a simple and highly versatile method using recombination-dependent exponential amplification.

scientific article published in September 2003

Cloning and in vitro and in vivo expression of plant glutathione S-transferase zeta class genes

scientific article published in January 2003

Crystal structure of glucansucrase from the dental caries pathogen Streptococcus mutans

scientific article

Crystallization and preliminary X-ray analysis of a glucansucrase from the dental caries pathogen Streptococcus mutans.

scientific article published on 28 August 2010

Emulsion culture: a miniaturized library screening system based on micro-droplets in an emulsified medium.

scientific article

Enhanced crossover SCRATCHY: construction and high-throughput screening of a combinatorial library containing multiple non-homologous crossovers.

scientific article

Evolution of highly active enzymes by homology-independent recombination

scientific article

Exhaustive identification of interaction domains using a high-throughput method based on two-hybrid screening and PCR-convergence: molecular dissection of a kinetochore subunit Spc34p.

scientific article

Export pathway selectivity of Escherichia coli twin arginine translocation signal peptides

scientific article published on 11 January 2007

High cell-density expression system: a novel method for extracellular production of difficult-to-express proteins.

scientific article

High cell-density expression system: yeast cells in a phalanx efficiently produce a certain range of "difficult-to-express" secretory recombinant proteins

scientific article

High-throughput, cloning-independent protein library construction by combining single-molecule DNA amplification with in vitro expression.

scientific article

Importance of disulfide bridge formation on folding of phospholipase D from Streptomyces antibioticus

scientific article published on 01 January 2000

In vitro protein biosynthesis using ribosome and foreign mRNA. An approach to construct a protein biosynthesizer

scientific article published on 01 March 1995

Phosphatase-immunodepleted cell-free protein synthesis system

scientific article

Quantitative Y2H screening: cloning and signal peptide engineering of a fungal secretory LacA gene and its application to yeast two-hybrid system as a quantitative reporter

scientific article

Regulation of RraA, a protein inhibitor of RNase E-mediated RNA decay

scientific article published on May 2006

Secretory expression of Lentinula edodes intracellular laccase by yeast high-cell-density system: sub-milligram production of difficult-to-express secretory protein

scientific article published on 7 January 2014

Signal peptide optimization tool for the secretion of recombinant protein from Saccharomyces cerevisiae.

scientific article

Soy peptides enhance heterologous membrane protein productivity during the exponential growth phase of Saccharomyces cerevisiae

scientific article published on 01 January 2012

Stabilization of affinity-tagged recombinant protein during/after its production in a cell-free system using wheat-germ extract

scientific article published in January 2003

Synergistic inhibition of human dipeptidyl peptidase IV by combinations of peptides

scholarly article by Masumi Ohno et al published July 2015 in Peptides

Systematic analysis of a dipeptide library for inhibitor development using human dipeptidyl peptidase IV produced by a Saccharomyces cerevisiae expression system ⬇️

scientific article published on December 23, 2012

Trp-Arg-Xaa tripeptides act as uncompetitive-type inhibitors of human dipeptidyl peptidase IV.

scientific article

List of works by Yasuaki Kawarasaki

A long-lived batch reaction system of cell-free protein synthesis

A method for functional mapping of protein-protein binding domain by preferential amplification of the shortest amplicon using PCR.

A method for reverse interactome analysis: High-resolution mapping of interdomain interaction network in Dam1 complex and its specific disorganization based on the interaction domain expression

A periplasmic fluorescent reporter protein and its application in high-throughput membrane protein topology analysis.

A trimmed viral cap-independent translation enhancing sequence for rapid in vitro gene expression

Analysing the substrate multispecificity of a proton-coupled oligopeptide transporter using a dipeptide library

Analyzing a dipeptide library to identify human dipeptidyl peptidase IV inhibitor.

Chimeric gene library construction by a simple and highly versatile method using recombination-dependent exponential amplification.

Cloning and in vitro and in vivo expression of plant glutathione S-transferase zeta class genes

Crystal structure of glucansucrase from the dental caries pathogen Streptococcus mutans

Crystallization and preliminary X-ray analysis of a glucansucrase from the dental caries pathogen Streptococcus mutans.

Emulsion culture: a miniaturized library screening system based on micro-droplets in an emulsified medium.

Enhanced crossover SCRATCHY: construction and high-throughput screening of a combinatorial library containing multiple non-homologous crossovers.

Evolution of highly active enzymes by homology-independent recombination

Exhaustive identification of interaction domains using a high-throughput method based on two-hybrid screening and PCR-convergence: molecular dissection of a kinetochore subunit Spc34p.

Export pathway selectivity of Escherichia coli twin arginine translocation signal peptides

High cell-density expression system: a novel method for extracellular production of difficult-to-express proteins.

High cell-density expression system: yeast cells in a phalanx efficiently produce a certain range of "difficult-to-express" secretory recombinant proteins

High-throughput, cloning-independent protein library construction by combining single-molecule DNA amplification with in vitro expression.

Importance of disulfide bridge formation on folding of phospholipase D from Streptomyces antibioticus

In vitro protein biosynthesis using ribosome and foreign mRNA. An approach to construct a protein biosynthesizer

Phosphatase-immunodepleted cell-free protein synthesis system

Quantitative Y2H screening: cloning and signal peptide engineering of a fungal secretory LacA gene and its application to yeast two-hybrid system as a quantitative reporter

Regulation of RraA, a protein inhibitor of RNase E-mediated RNA decay

Secretory expression of Lentinula edodes intracellular laccase by yeast high-cell-density system: sub-milligram production of difficult-to-express secretory protein

Signal peptide optimization tool for the secretion of recombinant protein from Saccharomyces cerevisiae.

Soy peptides enhance heterologous membrane protein productivity during the exponential growth phase of Saccharomyces cerevisiae

Stabilization of affinity-tagged recombinant protein during/after its production in a cell-free system using wheat-germ extract

Synergistic inhibition of human dipeptidyl peptidase IV by combinations of peptides

Systematic analysis of a dipeptide library for inhibitor development using human dipeptidyl peptidase IV produced by a Saccharomyces cerevisiae expression system ⬇️

Trp-Arg-Xaa tripeptides act as uncompetitive-type inhibitors of human dipeptidyl peptidase IV.