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List of works by Keiji Numata

3,4-Dihydroxyphenylalanine (DOPA)-Containing Silk Fibroin: Its Enzymatic Synthesis and Adhesion Properties

scientific article published on 01 March 2019

A Screening Method for the Isolation of Polyhydroxyalkanoate-Producing Purple Non-sulfur Photosynthetic Bacteria from Natural Seawater

scientific article

A centrifugation-assisted peptide-mediated gene transfer method for high-throughput analyses

scientific article published on 01 January 2019

A marine photosynthetic microbial cell factory as a platform for spider silk production

scientific article published on 08 July 2020

AFM Study of Morphology and Mechanical Properties of a Chimeric Spider Silk and Bone Sialoprotein Protein for Bone Regeneration ⬇️

scientific article published on March 31, 2011

Acetate-Inducing Metabolic States Enhance Polyhydroxyalkanoate Production in Marine Purple Non-sulfur Bacteria Under Aerobic Conditions.

scientific article published on 28 May 2019

Active intermediates of polyhydroxyalkanoate synthase from Aeromonas caviae in polymerization reaction

scientific article

Adsorption and hydrolysis reactions of poly(hydroxybutyric acid) depolymerases secreted from Ralstonia pickettii T1 and Penicillium funiculosum onto poly[(R)-3-hydroxybutyric acid]

scientific article published on 05 June 2007

Adsorption of biopolyester depolymerase on silicon wafer and poly[(R)-3-hydroxybutyric acid] single crystal revealed by real-time AFM.

scientific article published in January 2006

An oomycete NLP cytolysin forms transient small pores in lipid membranes

scientific article published in 2022

Analysis of repetitive amino acid motifs reveals the essential features of spider dragline silk proteins.

scientific article

Atomic resolution structure of serine protease proteinase K at ambient temperature

scientific article published on 31 March 2017

Biocompatible and biodegradable dual-drug release system based on silk hydrogel containing silk nanoparticles

scientific article published on 13 April 2012

Biodegradability of Poly(hydroxyalkanoate) Materials.

scientific article published on 28 August 2009

Bioengineered silk protein-based gene delivery systems

scientific article published on 4 July 2009

Biomaterials Science and Engineering in Japan: Attractive, Multidisciplinary, and Essential Research Field in Japan

scientific article published on 01 November 2019

Biopolymer-based nanoparticles for drug/gene delivery and tissue engineering.

scientific article published on 14 January 2013

Biosynthesis of polyhydroxyalkanaotes by a novel facultatively anaerobic Vibrio sp. under marine conditions

scientific article published on 09 November 2011

Biosynthesis of polyhydroxyalkanoate (PHA) copolymer from fructose using wild-type and laboratory-evolved PHA synthases.

scientific article

Biosynthesis of polyhydroxyalkanoates containing hydroxyl group from glycolate in Escherichia coli

scientific article published on 14 April 2016

Block Copolymer/Plasmid DNA Micelles Postmodified with Functional Peptides via Thiol-Maleimide Conjugation for Efficient Gene Delivery into Plants

scientific article published on 09 October 2018

Branched poly(lactide) synthesized by enzymatic polymerization: effects of molecular branches and stereochemistry on enzymatic degradation and alkaline hydrolysis

scientific article published on 28 August 2007

Carotenoids in the eyespot apparatus are required for triggering phototaxis in Euglena gracilis

scientific article published on 03 December 2019

Cell-Penetrating Peptide-Mediated Transformation of Large Plasmid DNA into Escherichia coli

scientific article published on 22 April 2019

Characterization of site-specific mutations in a short-chain-length/medium-chain-length polyhydroxyalkanoate synthase: in vivo and in vitro studies of enzymatic activity and substrate specificity

scientific article published on 12 April 2013

Characterization of the depolymerizing activity of commercial lipases and detection of lipase-like activities in animal organ extracts using poly(3-hydroxybutyrate-co-4-hydroxybutyrate) thin film

scientific article published on 12 October 2016

Chemo-Enzymatic Synthesis of Linear and Branched Cationic Peptides: Evaluation as Gene Carriers.

scientific article published on 31 March 2015

Chemoenzymatic Synthesis of Oligo(L-cysteine) for Use as a Thermostable Bio-Based Material.

scientific article published on 21 September 2015

Chemoenzymatic Synthesis of Polypeptides for Use as Functional and Structural Materials

scientific article

Chemoenzymatic synthesis of poly(L-alanine) in aqueous environment

scientific article published on 06 March 2012

Chemoenzymatic synthesis of polypeptides consisting of periodic di- and tri-peptide motifs similar to elastin

Chemoenzymatic synthesis of polypeptides containing the unnatural amino acid 2-aminoisobutyric acid.

scientific article published on 9 May 2017

Class I Polyhydroxyalkanoate Synthase from the Purple Photosynthetic Bacterium Rhodovulum sulfidophilum Predominantly Exists as a Functional Dimer in the Absence of a Substrate

scientific article published on 29 August 2017

Co-expression of two polyhydroxyalkanoate synthase subunits from Synechocystis sp. PCC 6803 by cell-free synthesis and their specific activity for polymerization of 3-hydroxybutyryl-coenzyme A.

scientific article published on 6 February 2015

Combination of Amorphous Silk Fiber Spinning and Postspinning Crystallization for Tough Regenerated Silk Fibers.

scientific article

Conformation and dynamics of soluble repetitive domain elucidates the initial β-sheet formation of spider silk.

scientific article

Development of reactive oxygen species-triggered degradable nanoparticles using oligoproline-containing peptides

scientific article published on 28 July 2020

Differences in Cytotoxicity of β‐Sheet Peptides Originated from Silk and Amyloid β ⬇️

scientific article published on October 15, 2010

Direct introduction of neomycin phosphotransferase II protein into apple leaves to confer kanamycin resistance

scientific article published on 26 November 2016

Dual peptide-based gene delivery system for the efficient transfection of plant callus cells

scientific article published on 20 May 2020

Efficient 3-hydroxybutyrate production by quiescent Escherichia coli microbial cell factories is facilitated by indole-induced proteomic and metabolomic changes.

scientific article published on 21 December 2017

Efficient callus induction and a temperature condition for flowering and seed setting in kenaf Hibiscus cannabinus

scientific article published on 01 March 2020

Enzymatic degradation of monolayer for poly(lactide) revealed by real-time atomic force microscopy: effects of stereochemical structure, molecular weight, and molecular branches on hydrolysis rates

scientific article published on 18 July 2008

Enzymatic degradation processes of lamellar crystals in thin films for poly[(R)-3-hydroxybutyric acid] and its copolymers revealed by real-time atomic force microscopy

scientific article published in November 2004

Enzymatic degradation processes of poly[(R)-3-hydroxybutyric acid] and poly[(R)-3-hydroxybutyric acid-co-(R)-3-hydroxyvaleric acid] single crystals revealed by atomic force microscopy: effects of molecular weight and second-monomer composition on er

scientific article published in July 2005

Enzyme-Mimic Peptide Assembly To Achieve Amidolytic Activity

scientific article published on 19 September 2016

Expression, cross-linking, and characterization of recombinant chitin binding resilin

scientific article published on December 2009

Gene delivery mediated by recombinant silk proteins containing cationic and cell binding motifs

scientific article

Gene introduction into the mitochondria of Arabidopsis thaliana via peptide-based carriers

scientific article published on 13 January 2015

Hydroxyethyl cellulose matrix applied to serial crystallography

scientific article

In-situ atomic force microscopy observation of enzymatic degradation in poly(hydroxyalkanoic acid) thin films: normal and constrained conditions.

scientific article published in March 2004

Increased bioplastic production with an RNA polymerase sigma factor SigE during nitrogen starvation in Synechocystis sp. PCC 6803.

scientific article published on 15 July 2013

Influence of Water Content on the β-Sheet Formation, Thermal Stability, Water Removal, and Mechanical Properties of Silk Materials.

scientific article published on 2 February 2016

Insights into the Stereospecificity in Papain-Mediated Chemoenzymatic Polymerization from Quantum Mechanics/Molecular Mechanics Simulations

scientific article published on 14 May 2019

Integrating tough Antheraea pernyi silk and strong carbon fibres for impact-critical structural composites

scientific article published on 22 August 2019

Intracellular Delivery of Proteins via Fusion Peptides in Intact Plants

scientific article

Ion effects on the conformation and dynamics of repetitive domains of a spider silk protein: implications for solubility and β-sheet formation

scientific article published on 01 August 2019

Library screening of cell-penetrating peptide for BY-2 cells, leaves of Arabidopsis, tobacco, tomato, poplar, and rice callus

scientific article published on 20 July 2018

Liquid Crystalline Granules Align in a Hierarchical Structure To Produce Spider Dragline Microfibrils.

scientific article published on 2 March 2017

Local gene silencing in plants via synthetic dsRNA and carrier peptide.

scientific article published on 6 June 2014

Marine Purple Photosynthetic Bacteria as Sustainable Microbial Production Hosts

scientific article published on 11 October 2019

Mechanism of enzymatic degradation of beta-sheet crystals

scientific article published on 30 December 2009

Mechanisms of enzymatic degradation of amyloid Beta microfibrils generating nanofilaments and nanospheres related to cytotoxicity

scientific article

Mechanistic insights into silk fibroin's adhesive properties via chemical functionalization of serine side chains

scientific article published on 03 October 2019

Method for the facile transformation of marine purple photosynthetic bacteria using chemically competent cells

scientific article published on 22 October 2019

Modification of β-oxidation pathway in Ralstonia eutropha for production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) from soybean oil.

scientific article published on 30 August 2013

Molecular Interactions and Toughening Mechanisms in Silk Fibroin-Epoxy Resin Blend Films

scientific article published on 23 May 2019

Monitoring and kinetic analysis of the molecular interactions by which a repressor protein, PhaR, binds to target DNAs and poly[(R)-3-hydroxybutyrate]

scientific article

Morphological and mechanical properties of flexible resilin joints on damselfly wings (Rhinocypha spp.).

scientific article published on 7 March 2018

Native protein delivery into rice callus using ionic complexes of protein and cell-penetrating peptides

scientific article published on 30 July 2019

Nearly complete 1H, 13C and 15N chemical shift assignment of monomeric form of N-terminal domain of Nephila clavipes major ampullate spidroin 2

scientific article published on 06 August 2020

Nonspecific hydrophobic interactions of a repressor protein, PhaR, with poly[(R)-3-hydroxybutyrate] film studied with a quartz crystal microbalance

scientific article published on 01 August 2006

Oil-free hyaluronic acid matrix for serial femtosecond crystallography

scientific article published on 18 April 2016

Optimal iron concentrations for growth-associated polyhydroxyalkanoate biosynthesis in the marine photosynthetic purple bacterium Rhodovulum sulfidophilum under photoheterotrophic condition

scientific article published on 29 April 2019

Optimization of poly(N-isopropylacrylamide) as an artificial amidase

scientific article published on 19 December 2014

Organelle-targeted gene delivery in plants by nanomaterials

scientific article published in 2023

Papain-Catalyzed Chemoenzymatic Synthesis of Telechelic Polypeptides Using Bis(Leucine Ethyl Ester) Initiator.

scientific article published on 7 March 2016

Papain-Catalyzed Synthesis of Polyglutamate Containing a Nylon Monomer Unit

scientific article published on 13 May 2016

Pathway-level acceleration of glycogen catabolism by a response regulator in the cyanobacterium Synechocystis species PCC 6803.

scientific article published on 12 February 2014

Peptide-Based Polyion Complex Vesicles That Deliver Enzymes into Intact Plants To Provide Antibiotic Resistance without Genetic Modification

scientific article published on 14 December 2020

Peptide-Mediated Gene Transfer into Marine Purple Photosynthetic Bacteria

scientific article published on 16 November 2020

Peptide-derived Method to Transport Genes and Proteins Across Cellular and Organellar Barriers in Plants

scientific article

Phasin proteins activate Aeromonas caviae polyhydroxyalkanoate (PHA) synthase but not Ralstonia eutropha PHA synthase.

scientific article published on 28 February 2014

Polymer-coated carbon nanotube hybrids with functional peptides for gene delivery into plant mitochondria

scientific article published on 16 May 2022

Proteinase K-catalyzed synthesis of linear and star oligo(L-phenylalanine) conjugates

scientific article

Quantifying osteogenic cell degradation of silk biomaterials

scientific article published on 24 November 2010

Raman image-activated cell sorting

scientific article published on 10 July 2020

Rapid and efficient gene delivery into plant cells using designed peptide carriers

article by Manoj Lakshmanan et al published 14 December 2012 in Biomacromolecules

Recent advances in chemoenzymatic peptide syntheses.

scientific article published on 03 September 2014

Reinforcing silk scaffolds with silk particles.

scientific article

Relationships between physical properties and sequence in silkworm silks

scientific article published on 09 June 2016

Revealing the Architecture of the Cell Wall in Living Plant Cells by Bioimaging and Enzymatic Degradation

scientific article published on 20 September 2019

Role of Skin Layers on Mechanical Properties and Supercontraction of Spider Dragline Silk Fiber

scientific article published on 19 September 2018

Selective Gene Delivery for Integrating Exogenous DNA into Plastid and Mitochondrial Genomes Using Peptide-DNA Complexes

scientific article published on 05 April 2018

Self-Assembled Peptide-Based System for Mitochondrial-Targeted Gene Delivery: Functional and Structural Insights

scientific article published on 03 October 2016

Short one-pot chemo-enzymatic synthesis of L-lysine and L-alanine diblock co-oligopeptides

scientific article

Silk Composite with a Fluoropolymer as a Water-Resistant Protein-Based Material

scientific article published on 21 April 2018

Silk Resin with Hydrated Dual Chemical-Physical Cross-Links Achieves High Strength and Toughness

scientific article published on 27 April 2017

Silk-Based Gene Carriers with Cell Membrane Destabilizing Peptides ⬇️

scientific article published on October 13, 2010

Silk-based delivery systems of bioactive molecules.

scientific article published on 16 March 2010

Silk-based nanocomplexes with tumor-homing peptides for tumor-specific gene delivery

scientific article

Silk-pectin hydrogel with superior mechanical properties, biodegradability, and biocompatibility.

scientific article published on 7 March 2014

Silk/Natural Rubber (NR) and 3,4-Dihydroxyphenylalanine (DOPA)-Modified Silk/NR Composites: Synthesis, Secondary Structure, and Mechanical Properties

scientific article published on 06 January 2020

Simultaneous introduction of multiple biomacromolecules into plant cells using a cell-penetrating peptide nanocarrier

scientific article published on 08 September 2020

Spider dragline silk composite films doped with linear and telechelic polyalanine: Effect of polyalanine on the structure and mechanical properties.

scientific article published on 26 February 2018

Spider silk self-assembly via modular liquid-liquid phase separation and nanofibrillation

scientific article published on 04 November 2020

Spider silk-based gene carriers for tumor cell-specific delivery.

scientific article

State of water, molecular structure, and cytotoxicity of silk hydrogels.

scientific article published on 9 May 2011

Stimulus-Responsive Peptide for Effective Delivery and Release of DNA in Plants

scientific article published on 2 March 2018

Sucrose supplementation suppressed the growth inhibition in polyhydroxyalkanoate-producing plants

scientific article published on 14 February 2017

Surface Analysis of Native Spider Draglines by FE-SEM and XPS

scientific article published on 20 March 2020

Surface-patterned electrode bioreactor for electrical stimulation

scientific article

Synthesis of High-Molecular-Weight Polyhydroxyalkanoates by Marine Photosynthetic Purple Bacteria

scientific article

Synthesis of adhesive peptides similar to those found in blue mussel (Mytilus edulis) using papain and tyrosinase

scientific article published on 23 July 2014

Synthesis of homopolypeptides by aminolysis mediated by proteases encapsulated in silica nanospheres.

scientific article published on 25 August 2014

Targeted Gene Delivery into Various Plastids Mediated by Clustered Cell-Penetrating and Chloroplast-Targeting Peptides

scientific article published on 23 October 2019

Targeted Gene Delivery: Targeted Gene Delivery into Various Plastids Mediated by Clustered Cell‐Penetrating and Chloroplast‐Targeting Peptides (Adv. Sci. 23/2019)

Tensile Reinforcement of Silk Films by the Addition of Telechelic-Type Polyalanine

scientific article published on 24 January 2017

The Benzyl Ester Group of Amino Acid Monomers Enhances Substrate Affinity and Broadens the Substrate Specificity of the Enzyme Catalyst in Chemoenzymatic Copolymerization

scientific article published on 30 November 2015

The Biomedical Use of Silk: Past, Present, Future

The bagworm genome reveals a unique fibroin gene that provides high tensile strength

article

The interplay between silk fibroin's structure and its adhesive properties

scientific article published on 20 July 2018

Two Birds with One Stone: Spontaneous Size Separation and Growth Inhibition of Femtosecond Laser-Generated Surfactant-Free Metallic Nanoparticles via ex Situ SU-8 Functionalization

scientific article published on 11 September 2018

Use of extension-deformation-based crystallisation of silk fibres to differentiate their functions in nature

scientific article published on 01 August 2015

Vacuum/Compression Infiltration-mediated Permeation Pathway of a Peptide-pDNA Complex as a Non-Viral Carrier for Gene Delivery in Planta

scientific article published in Scientific Reports

Viscosity-adjustable grease matrices for serial nanocrystallography

scientific article published on 28 January 2020

Zwitterionic Polypeptides: Chemoenzymatic Synthesis and Loosening Function for Cellulose Crystals

scientific article published on 24 January 2020

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