List of works - Takashi Umehara

A series of bacterial co-expression vectors with rare-cutter recognition sequences.

scientific article

A tandem insertion vector for large-scale preparation of nucleosomal DNA

scientific article published in 2012

Acetylated histone H4 tail enhances histone H3 tail acetylation by altering their mutual dynamics in the nucleosome

scientific article published on 03 August 2020

Activation of lysine-specific demethylase 1 inhibitor peptide by redox-controlled cleavage of a traceless linker.

scientific article published on 23 December 2016

Cell death with predominant apoptotic features in Saccharomyces cerevisiae mediated by deletion of the histone chaperone ASF1/CIA1.

scientific article published in December 2001

Cell-permeable Carboxyl-terminal p27Kip1 Peptide Exhibits Anti-tumor Activity by Inhibiting Pim-1 Kinase

scientific article

Characteristic H3 N-tail dynamics in the nucleosome core particle, nucleosome, and chromatosome

scientific article published on 17 February 2022

Characterization of lysine acetylation of a phosphoenolpyruvate carboxylase involved in glutamate overproduction in Corynebacterium glutamicum.

scientific article

Chromosomal gradient of histone acetylation established by Sas2p and Sir2p functions as a shield against gene silencing

scientific article published on 15 October 2002

Crystal Structure of LSD1 in Complex with 4-[5-(Piperidin-4-ylmethoxy)-2-(p-tolyl)pyridin-3-yl]benzonitrile.

scientific article published on 26 June 2018

Crystal structure analysis of the PHD domain of the transcription co-activator Pygopus.

scientific article published on 20 April 2007

Crystal structure of RNA polymerase II from Komagataella pastoris

scientific article published on 12 April 2017

Crystal structure of eukaryotic translation initiation factor 2B

scientific article

Crystal structure of histone demethylase LSD1 and tranylcypromine at 2.25 A

scientific article

Crystal structure of human nucleosome core particle containing enzymatically introduced CpG methylation

scientific article published on 3 May 2016

Crystal structure of the human BRD2 bromodomain: insights into dimerization and recognition of acetylated histone H4.

scientific article published on 5 December 2006

Crystal structures of fission yeast histone chaperone Asf1 complexed with the Hip1 B-domain or the Cac2 C terminus

scientific article

Crystallization of the archaeal transcription termination factor NusA: a significant decrease in twinning under microgravity conditions

scientific article

Crystallographic study of a site-specifically cross-linked protein complex with a genetically incorporated photoreactive amino acid

scientific article

Development and Structural Evaluation of N-Alkylated trans-2-Phenylcyclopropylamine-Based LSD1 Inhibitors

scientific article published on 12 March 2020

Development and crystallographic evaluation of histone H3 peptide with N-terminal serine substitution as a potent inhibitor of lysine-specific demethylase 1.

scientific article

Development of Novel Inhibitors for Histone Methyltransferase SET7/9 based on Cyproheptadine

scientific article published on 06 July 2018

Development of a hexahistidine-3× FLAG-tandem affinity purification method for endogenous protein complexes in Pichia pastoris

scientific article published on 15 November 2014

Discovery of Novel Spiroindoline Derivatives as Selective Tankyrase Inhibitors

scientific article published on 01 April 2019

Distribution of histone H4 modifications as revealed by a panel of specific monoclonal antibodies

scientific article

Eradication of Central Nervous System Leukemia of T-Cell Origin with a Brain-Permeable LSD1 Inhibitor

scientific article published on 05 December 2018

Expanded genetic code technologies for incorporating modified lysine at multiple sites

scientific article published on September 2014

FAD-dependent lysine-specific demethylase-1 regulates cellular energy expenditure

scientific article

Genetic-code evolution for protein synthesis with non-natural amino acids.

scientific article

Global analysis of functional surfaces of core histones with comprehensive point mutants

scientific article published in January 2007

Histone H4 lysine 20 acetylation is associated with gene repression in human cells

scientific article

IRS-1 acts as an endocytic regulator of IGF-I receptor to facilitate sustained IGF signaling

scientific article published on 11 April 2018

Identification of Cyproheptadine as an Inhibitor of SET Domain Containing Lysine Methyltransferase 7/9 (Set7/9) That Regulates Estrogen-Dependent Transcription

scientific article

Intra- and inter-nucleosomal interactions of the histone H4 tail revealed with a human nucleosome core particle with genetically-incorporated H4 tetra-acetylation

scientific article

Isolation and characterization of a cDNA encoding a new type of human transcription elongation factor S-II

scientific article

Isolation of a cDNA encoding a mouse TFIID subunit containing histone H4 homology

scientific article

JQ1 affects BRD2-dependent and independent transcription regulation without disrupting H4-hyperacetylated chromatin states

scientific article published on 06 August 2018

Lysine-specific demethylase 1 inhibitors prevent teratoma development from human induced pluripotent stem cells.

scientific article published on 8 January 2018

Lysine-specific demethylase 2 suppresses lipid influx and metabolism in hepatic cells

scientific article published on 26 January 2015

Mapping of the basic amino-acid residues responsible for tubulation and cellular protrusion by the EFC/F-BAR domain of pacsin2/Syndapin II

scientific article

Multiple Site-Specific Installations ofNε-Monomethyl-L-Lysine into Histone Proteins by Cell-Based and Cell-Free Protein Synthesis

scientific article published in 2014

Polyanionic stretch-deleted histone chaperone cia1/Asf1p is functional both in vivo and in vitro

scientific article published in January 2002

Purification, crystallization and preliminary X-ray diffraction analysis of the histone chaperone cia1 from fission yeast

scientific article

Purification, crystallization and preliminary X-ray diffraction analysis of the non-ATPase subunit Nas6 in complex with the ATPase subunit Rpt3 of the 26S proteasome from Saccharomyces cerevisiae

scientific article published on 10 February 2007

Purification, crystallization and preliminary X-ray diffraction of the C-terminal bromodomain from human BRD2.

scientific article published on 22 June 2007

Quantification of the effect of site-specific histone acetylation on chromatin transcription rate

scientific article published on 26 November 2020

Rational design and implementation of a chemically inducible heterotrimerization system

scientific article published on 03 August 2020

Real-time imaging of histone H4K12-specific acetylation determines the modes of action of histone deacetylase and bromodomain inhibitors

scientific article

Restricted expression of a member of the transcription elongation factor S-II family in testicular germ cells during and after meiosis

scientific article published in March 1997

ST1710-DNA complex crystal structure reveals the DNA binding mechanism of the MarR family of regulators

scientific article

Solution structure of histone chaperone ANP32B: interaction with core histones H3-H4 through its acidic concave domain

scientific article

Solution structure of the SWIRM domain of human histone demethylase LSD1

scientific article

Solution structure of the extraterminal domain of the bromodomain-containing protein BRD4.

scientific article published on 24 September 2008

Solution structure of the zinc finger HIT domain in protein FON

scientific article

Solution structures of the DNA-binding domains of immune-related zinc-finger protein ZFAT.

scientific article published on 24 March 2015

Structural Basis for Acetylated Histone H4 Recognition by the Human BRD2 Bromodomain

scientific article

Structural and functional differences of SWIRM domain subtypes

scientific article

Structural basis for the recognition between the regulatory particles Nas6 and Rpt3 of the yeast 26S proteasome

scientific article

Structural implications for K5/K12-di-acetylated histone H4 recognition by the second bromodomain of BRD2

scientific article

Structural insight into inhibitors of flavin adenine dinucleotide-dependent lysine demethylases

scientific article published on 10 February 2017

Structural insight into the zinc finger CW domain as a histone modification reader

scientific article

Structural similarity between histone chaperone Cia1p/Asf1p and DNA-binding protein NF-kappaB.

scientific article

Structurally designed trans-2-phenylcyclopropylamine derivatives potently inhibit histone demethylase LSD1/KDM1

scientific article

Structure of the oncoprotein gankyrin in complex with S6 ATPase of the 26S proteasome

scientific article

Structures of histone methyltransferase SET7/9 in complexes with adenosylmethionine derivatives

scientific article

Temperature-Sensitive Substrate and Product Binding Underlie Temperature-Compensated Phosphorylation in the Clock

scientific article published in September 2017

The methyltransferase METTL9 mediates pervasive 1-methylhistidine modification in mammalian proteomes

scientific article published on 09 February 2021

Three distinct regions in a rat TFIID subunit containing histone H4 homology

scientific article

Transcription initiation factor IID-interactive histone chaperone CIA-II implicated in mammalian spermatogenesis

scientific article (publication date: 12 September 2003)

Tri-methylation of ATF7IP by G9a/GLP recruits the chromodomain protein MPP8

scientific article published on 04 October 2018

[New frontiers of epigenetics researches in pharmaceutical sciences]

scientific article published on 01 January 2015

[New theory on gene regulation: mechanism of formation of chromosome functional and boundary regions]

scientific article published on 01 March 2003

List of works by Takashi Umehara

A series of bacterial co-expression vectors with rare-cutter recognition sequences.

A tandem insertion vector for large-scale preparation of nucleosomal DNA

Acetylated histone H4 tail enhances histone H3 tail acetylation by altering their mutual dynamics in the nucleosome

Activation of lysine-specific demethylase 1 inhibitor peptide by redox-controlled cleavage of a traceless linker.

Cell death with predominant apoptotic features in Saccharomyces cerevisiae mediated by deletion of the histone chaperone ASF1/CIA1.

Cell-permeable Carboxyl-terminal p27Kip1 Peptide Exhibits Anti-tumor Activity by Inhibiting Pim-1 Kinase

Characteristic H3 N-tail dynamics in the nucleosome core particle, nucleosome, and chromatosome

Characterization of lysine acetylation of a phosphoenolpyruvate carboxylase involved in glutamate overproduction in Corynebacterium glutamicum.

Chromosomal gradient of histone acetylation established by Sas2p and Sir2p functions as a shield against gene silencing

Crystal Structure of LSD1 in Complex with 4-[5-(Piperidin-4-ylmethoxy)-2-(p-tolyl)pyridin-3-yl]benzonitrile.

Crystal structure analysis of the PHD domain of the transcription co-activator Pygopus.

Crystal structure of RNA polymerase II from Komagataella pastoris

Crystal structure of eukaryotic translation initiation factor 2B

Crystal structure of histone demethylase LSD1 and tranylcypromine at 2.25 A

Crystal structure of human nucleosome core particle containing enzymatically introduced CpG methylation

Crystal structure of the human BRD2 bromodomain: insights into dimerization and recognition of acetylated histone H4.

Crystal structures of fission yeast histone chaperone Asf1 complexed with the Hip1 B-domain or the Cac2 C terminus

Crystallization of the archaeal transcription termination factor NusA: a significant decrease in twinning under microgravity conditions

Crystallographic study of a site-specifically cross-linked protein complex with a genetically incorporated photoreactive amino acid

Development and Structural Evaluation of N-Alkylated trans-2-Phenylcyclopropylamine-Based LSD1 Inhibitors

Development and crystallographic evaluation of histone H3 peptide with N-terminal serine substitution as a potent inhibitor of lysine-specific demethylase 1.

Development of Novel Inhibitors for Histone Methyltransferase SET7/9 based on Cyproheptadine

Development of a hexahistidine-3× FLAG-tandem affinity purification method for endogenous protein complexes in Pichia pastoris

Discovery of Novel Spiroindoline Derivatives as Selective Tankyrase Inhibitors

Distribution of histone H4 modifications as revealed by a panel of specific monoclonal antibodies

Eradication of Central Nervous System Leukemia of T-Cell Origin with a Brain-Permeable LSD1 Inhibitor

Expanded genetic code technologies for incorporating modified lysine at multiple sites

FAD-dependent lysine-specific demethylase-1 regulates cellular energy expenditure

Genetic-code evolution for protein synthesis with non-natural amino acids.

Global analysis of functional surfaces of core histones with comprehensive point mutants

Histone H4 lysine 20 acetylation is associated with gene repression in human cells

IRS-1 acts as an endocytic regulator of IGF-I receptor to facilitate sustained IGF signaling

Identification of Cyproheptadine as an Inhibitor of SET Domain Containing Lysine Methyltransferase 7/9 (Set7/9) That Regulates Estrogen-Dependent Transcription

Intra- and inter-nucleosomal interactions of the histone H4 tail revealed with a human nucleosome core particle with genetically-incorporated H4 tetra-acetylation

Isolation and characterization of a cDNA encoding a new type of human transcription elongation factor S-II

Isolation of a cDNA encoding a mouse TFIID subunit containing histone H4 homology

JQ1 affects BRD2-dependent and independent transcription regulation without disrupting H4-hyperacetylated chromatin states

Lysine-specific demethylase 1 inhibitors prevent teratoma development from human induced pluripotent stem cells.

Lysine-specific demethylase 2 suppresses lipid influx and metabolism in hepatic cells

Mapping of the basic amino-acid residues responsible for tubulation and cellular protrusion by the EFC/F-BAR domain of pacsin2/Syndapin II

Multiple Site-Specific Installations ofNε-Monomethyl-L-Lysine into Histone Proteins by Cell-Based and Cell-Free Protein Synthesis

Polyanionic stretch-deleted histone chaperone cia1/Asf1p is functional both in vivo and in vitro

Purification, crystallization and preliminary X-ray diffraction analysis of the histone chaperone cia1 from fission yeast

Purification, crystallization and preliminary X-ray diffraction analysis of the non-ATPase subunit Nas6 in complex with the ATPase subunit Rpt3 of the 26S proteasome from Saccharomyces cerevisiae

Purification, crystallization and preliminary X-ray diffraction of the C-terminal bromodomain from human BRD2.

Quantification of the effect of site-specific histone acetylation on chromatin transcription rate

Rational design and implementation of a chemically inducible heterotrimerization system

Real-time imaging of histone H4K12-specific acetylation determines the modes of action of histone deacetylase and bromodomain inhibitors

Restricted expression of a member of the transcription elongation factor S-II family in testicular germ cells during and after meiosis

ST1710-DNA complex crystal structure reveals the DNA binding mechanism of the MarR family of regulators

Solution structure of histone chaperone ANP32B: interaction with core histones H3-H4 through its acidic concave domain

Solution structure of the SWIRM domain of human histone demethylase LSD1

Solution structure of the extraterminal domain of the bromodomain-containing protein BRD4.

Solution structure of the zinc finger HIT domain in protein FON

Solution structures of the DNA-binding domains of immune-related zinc-finger protein ZFAT.

Structural Basis for Acetylated Histone H4 Recognition by the Human BRD2 Bromodomain

Structural and functional differences of SWIRM domain subtypes

Structural basis for the recognition between the regulatory particles Nas6 and Rpt3 of the yeast 26S proteasome

Structural implications for K5/K12-di-acetylated histone H4 recognition by the second bromodomain of BRD2

Structural insight into inhibitors of flavin adenine dinucleotide-dependent lysine demethylases

Structural insight into the zinc finger CW domain as a histone modification reader

Structural similarity between histone chaperone Cia1p/Asf1p and DNA-binding protein NF-kappaB.

Structurally designed trans-2-phenylcyclopropylamine derivatives potently inhibit histone demethylase LSD1/KDM1

Structure of the oncoprotein gankyrin in complex with S6 ATPase of the 26S proteasome

Structures of histone methyltransferase SET7/9 in complexes with adenosylmethionine derivatives

Temperature-Sensitive Substrate and Product Binding Underlie Temperature-Compensated Phosphorylation in the Clock

The methyltransferase METTL9 mediates pervasive 1-methylhistidine modification in mammalian proteomes

Three distinct regions in a rat TFIID subunit containing histone H4 homology

Transcription initiation factor IID-interactive histone chaperone CIA-II implicated in mammalian spermatogenesis

Tri-methylation of ATF7IP by G9a/GLP recruits the chromodomain protein MPP8

[New frontiers of epigenetics researches in pharmaceutical sciences]

[New theory on gene regulation: mechanism of formation of chromosome functional and boundary regions]