List of works - Lance C Seefeldt

57Fe ENDOR spectroscopy and 'electron inventory' analysis of the nitrogenase E4 intermediate suggest the metal-ion core of FeMo-cofactor cycles through only one redox couple

scientific article

A Continuous, Spectrophotometric Activity Assay for Nitrogenase Using the Reductant Titanium(III) Citrate

scientific article published on 01 September 1994

A Mediated Thin-Layer Voltammetry Method for the Study of Redox Protein Electrochemistry

scientific article published on 01 April 1997

A confirmation of the quench-cryoannealing relaxation protocol for identifying reduction states of freeze-trapped nitrogenase intermediates

scientific article

A conformational mimic of the MgATP-bound "on state" of the nitrogenase iron protein

scientific article

A substrate channel in the nitrogenase MoFe protein

scientific article

Aerobic, inactive forms of Azotobacter vinelandii hydrogenase: activation kinetics and insensitivity to C2H2 inhibition

scientific article published in November 1988

Alkyne substrate interaction within the nitrogenase MoFe protein

scientific article published on 29 May 2007

An Efficient Viologen-Based Electron Donor to Nitrogenase

scientific article published on 08 November 2019

Analysis of a gene region required for dihydrogen oxidation in Azotobacter vinelandii

article

Beyond fossil fuel-driven nitrogen transformations

scientific article published in Science

Biodiesel production by simultaneous extraction and conversion of total lipids from microalgae, cyanobacteria, and wild mixed-cultures

article

Breaking the N2 triple bond: insights into the nitrogenase mechanism

scientific article

CO2 Reduction Catalyzed by Nitrogenase: Pathways to Formate, Carbon Monoxide, and Methane

scientific article published on 8 August 2016

Catalytic and biophysical properties of a nitrogenase Apo-MoFe protein produced by a nifB-deletion mutant of Azotobacter vinelandii

scientific article published in September 1998

Changes in the Midpoint Potentials of the Nitrogenase Metal Centers as a Result of Iron Protein−Molybdenum-Iron Protein Complex Formation†

article

Characterization of a Fatty Acyl-CoA Reductase fromMarinobacter aquaeoleiVT8: A Bacterial Enzyme Catalyzing the Reduction of Fatty Acyl-CoA to Fatty Alcohol

article

Cluster Dependent Charge-Transfer Dynamics in Iron-Sulfur Proteins.

scientific article published on 5 January 2018

Competitive substrate and inhibitor interactions at the physiologically relevant active site of nitrogenase

scientific article published in November 2000

Conformational gating of electron transfer from the nitrogenase Fe protein to MoFe protein

scientific article published on May 2010

Connecting nitrogenase intermediates with the kinetic scheme for N2 reduction by a relaxation protocol and identification of the N2 binding state.

scholarly article

Construction and characterization of a heterodimeric iron protein: defining roles for adenosine triphosphate in nitrogenase catalysis.

scientific article published in June 2000

Control of electron transfer in nitrogenase

scientific article published on 08 September 2018

Correction to "Establishing a Thermodynamic Landscape for the Active Site of Mo-Dependent Nitrogenase"

scientific article published on 26 March 2020

Correction to "High-Resolution ENDOR Spectroscopy Combined with Quantum Chemical Calculations Reveals the Structure of Nitrogenase Janus Intermediate E4(4H)"

scientific article published on 10 December 2019

Crystal structure of the L protein of Rhodobacter sphaeroides light-independent protochlorophyllide reductase with MgADP bound: a homologue of the nitrogenase Fe protein

scientific article

Defining Intermediates of Nitrogenase MoFe Protein during N2 Reduction under Photochemical Electron Delivery from CdS Quantum Dots

scientific article published on 05 August 2020

Determination of rate and equilibrium constants for the reactions between electron transfer mediators and proteins by linear sweep voltammetry.

scientific article published in July 1997

Diazene (HN=NH) is a substrate for nitrogenase: insights into the pathway of N2 reduction.

scientific article published on 18 May 2007

ENDOR/HYSCORE studies of the common intermediate trapped during nitrogenase reduction of N2H2, CH3N2H, and N2H4 support an alternating reaction pathway for N2 reduction

scientific article published on 11 July 2011

EXAFS and NRVS reveal a conformational distortion of the FeMo-cofactor in the MoFe nitrogenase propargyl alcohol complex

scientific article

Electrocatalytic CO2 reduction catalyzed by nitrogenase MoFe and FeFe proteins.

scientific article published on 5 December 2017

Electron Paramagnetic Resonance Spectroscopy

article

Electron Redistribution within the Nitrogenase Active Site FeMo-Cofactor During Reductive Elimination of H<sub>2</sub> to Achieve N≡N Triple-Bond Activation

scientific article published on 16 December 2020

Electron Transfer from the Nitrogenase Iron Protein to the [8Fe-(7/8)S] Clusters of the Molybdenum−Iron Protein†

article

Electron Transfer in Nitrogenase Analyzed by Marcus Theory: Evidence for Gating by MgATP†

article

Electron transfer in nitrogenase catalysis.

scientific article published on 5 March 2012

Electron transfer precedes ATP hydrolysis during nitrogenase catalysis.

scientific article published on 23 September 2013

Electron transfer within nitrogenase: evidence for a deficit-spending mechanism

scientific article published on 11 October 2011

Elucidating the Mechanism of Nucleotide-Dependent Changes in the Redox Potential of the [4Fe-4S] Cluster in Nitrogenase Iron Protein: The Role of Phenylalanine 135†

article

Energy Transduction in Nitrogenase

scientific article published on 10 August 2018

Entropies of Redox Reactions between Proteins and Mediators: The Temperature Dependence of Reversible Electrode Potentials in Aqueous Buffers ⬇️

scientific article published on August 1, 1997

Establishing a Thermodynamic Landscape for the Active Site of Mo-Dependent Nitrogenase

scientific article published on 17 October 2019

Evidence That MgATP Accelerates Primary Electron Transfer in aClostridium pasteurianumFe Protein-Azotobacter vinelandiiMoFe Protein Nitrogenase Tight Complex

scientific article published in Journal of Biological Chemistry

Evidence That the Pi Release Event Is the Rate-Limiting Step in the Nitrogenase Catalytic Cycle

scientific article published on 13 June 2016

Evidence for Electron Transfer from the Nitrogenase Iron Protein to the Molybdenum−Iron Protein without MgATP Hydrolysis: Characterization of a Tight Protein−Protein Complex†

article

Evidence for Electron Transfer-dependent Formation of a Nitrogenase Iron Protein-Molybdenum-Iron Protein Tight Complex

scientific article published in Journal of Biological Chemistry

Evidence for coupled electron and proton transfer in the [8Fe-7S] cluster of nitrogenase

scientific article published in August 1998

Exploring Electron/Proton Transfer and Conformational Changes in the Nitrogenase MoFe Protein and FeMo-cofactor Through Cryoreduction/EPR Measurements.

scientific article published on 29 July 2016

Exploring the alternatives of biological nitrogen fixation.

scientific article published on 9 April 2018

Fe protein-independent substrate reduction by nitrogenase MoFe protein variants

scientific article

Frontiers, opportunities, and challenges in biochemical and chemical catalysis of CO2 fixation

scientific article (publication date: 14 August 2013)

High-Resolution ENDOR Spectroscopy Combined with Quantum Chemical Calculations Reveals the Structure of Nitrogenase Janus Intermediate E4(4H)

scientific article published on 16 July 2019

Hydride Conformers of the Nitrogenase FeMo-cofactor Two-Electron Reduced State E2(2H), Assigned Using Cryogenic Intra Electron Paramagnetic Resonance Cavity Photolysis.

scientific article published on 24 March 2018

Hydrolysis of Nucleoside Triphosphates Other than ATP by Nitrogenase

scientific article published in Journal of Biological Chemistry

Identification of a key catalytic intermediate demonstrates that nitrogenase is activated by the reversible exchange of N₂ for H₂.

scientific article published on 5 March 2015

Improving energetics of triacylglyceride extraction from wet oleaginous microbes.

scientific article published on 14 June 2014

Infrared spectroscopy of the nitrogenase MoFe protein under electrochemical control: potential-triggered CO binding.

scientific article published on 27 October 2016

Insights into nucleotide signal transduction in nitrogenase: structure of an iron protein with MgADP bound

scientific article

Insights into substrate binding at FeMo-cofactor in nitrogenase from the structure of an alpha-70(Ile) MoFe protein variant

scientific article

Insights into the role of nucleotide-dependent conformational change in nitrogenase catalysis: Structural characterization of the nitrogenase Fe protein Leu127 deletion variant with bound MgATP

article

Interaction of acetylene and cyanide with the resting state of nitrogenase alpha-96-substituted MoFe proteins

scientific article published in November 2001

Intermediates Trapped during Nitrogenase Reduction of N⋮N, CH3−NNH, and H2N−NH2

article

Isolation and characterization of an acetylene-resistant nitrogenase

scientific article published in April 2000

Light-driven dinitrogen reduction catalyzed by a CdS:nitrogenase MoFe protein biohybrid

scientific article

Localization of a catalytic intermediate bound to the FeMo-cofactor of nitrogenase

scientific article

Localization of a substrate binding site on the FeMo-cofactor in nitrogenase: trapping propargyl alcohol with an alpha-70-substituted MoFe protein

scientific article published in August 2003

Mechanism of Mo-Dependent Nitrogenase

article

Mechanism of Nitrogenase H2 Formation by Metal-Hydride Protonation Probed by Mediated Electrocatalysis and H/D Isotope Effects.

scientific article published on 29 August 2017

Mechanism of nitrogen fixation by nitrogenase: the next stage

scientific article

Mo-, V-, and Fe-Nitrogenases Use a Universal Eight-Electron Reductive-Elimination Mechanism To Achieve N2 Reduction

scientific article published on 19 July 2019

Molybdenum nitrogenase catalyzes the reduction and coupling of CO to form hydrocarbons

scientific article

Negative cooperativity in the nitrogenase Fe protein electron delivery cycle.

scientific article published on October 2016

Nitrite and hydroxylamine as nitrogenase substrates: mechanistic implications for the pathway of N₂ reduction

scientific article published on 28 August 2014

Nitrogen fixation: the mechanism of the Mo-dependent nitrogenase.

scientific article

Nitrogenase Reduction of Carbon Disulfide: Freeze-Quench EPR and ENDOR Evidence for Three Sequential Intermediates with Cluster-Bound Carbon Moieties†

article

Nitrogenase: a draft mechanism

scientific article

Oleaginous yeast platform for producing biofuels via co-solvent hydrothermal liquefaction

scientific article

On reversible H2 loss upon N2 binding to FeMo-cofactor of nitrogenase.

scientific article

Photoinduced Reductive Elimination of H2 from the Nitrogenase Dihydride (Janus) State Involves a FeMo-cofactor-H2 Intermediate

scientific article published on 8 February 2017

Probing the MgATP-bound conformation of the nitrogenase Fe protein by solution small-angle X-ray scattering

scientific article published on 15 November 2007

Purification, characterization, and potential bacterial wax production role of an NADPH-dependent fatty aldehyde reductase from Marinobacter aquaeolei VT8.

scientific article published on 6 March 2009

Reduction of Substrates by Nitrogenases

scientific article published on 16 March 2020

Reduction of Thiocyanate, Cyanate, and Carbon Disulfide by Nitrogenase: Kinetic Characterization and EPR Spectroscopic Analysis ⬇️

scientific article published on July 15, 1997

Reductive Elimination of H2 Activates Nitrogenase to Reduce the N≡N Triple Bond: Characterization of the E4(4H) Janus Intermediate in Wild-Type Enzyme

scientific article published on 16 August 2016

Reversible Photoinduced Reductive Elimination of H2 from the Nitrogenase Dihydride State, the E(4)(4H) Janus Intermediate.

scientific article

Spectroscopic Description of the E1 State of Mo Nitrogenase Based on Mo and Fe X-ray Absorption and Mössbauer Studies

scientific article published on 23 August 2019

Stereospecificity of acetylene reduction catalyzed by nitrogenase

scientific article published in March 2001

Steric control of the Hi-CO MoFe nitrogenase complex revealed by stopped-flow infrared spectroscopy

scientific article

Structural and biochemical implications of single amino acid substitutions in the nucleotide-dependent switch regions of the nitrogenase Fe protein from Azotobacter vinelandii

scientific article published on 10 November 2004

Substrate Channel in Nitrogenase Revealed by a Molecular Dynamics Approach

article

Substrate interaction at an iron-sulfur face of the FeMo-cofactor during nitrogenase catalysis

scientific article published in October 2004

Substrate interactions with nitrogenase: Fe versus Mo.

scientific article published in February 2004

Substrate interactions with the nitrogenase active site

scientific article

Techno-economic feasibility and life cycle assessment of dairy effluent to renewable diesel via hydrothermal liquefaction.

scientific article

Temperature invariance of the nitrogenase electron transfer mechanism

scientific article published on 10 October 2012

Testing if the interstitial atom, X, of the nitrogenase molybdenum-iron cofactor is N or C: ENDOR, ESEEM, and DFT studies of the S = 3/2 resting state in multiple environments

scientific article published on 21 November 2007

The Electron Bifurcating FixABCX Protein Complex from Azotobacter vinelandii: Generation of Low-Potential Reducing Equivalents for Nitrogenase Catalysis.

scientific article published on 13 July 2017

The Interstitial Atom of the Nitrogenase FeMo-Cofactor: ENDOR and ESEEM Evidence That it is Not a Nitrogen

article

The Interstitial Atom of the Nitrogenase FeMo-Cofactor: ENDOR and ESEEM Show It Is Not an Exchangeable Nitrogen

article

The Mechanism of N2 Reduction Catalyzed by Fe-Nitrogenase Involves Reductive Elimination of H2.

scientific article

The NifZ accessory protein has an equivalent function in maturation of both nitrogenase MoFe protein P-clusters

scientific article published on 07 March 2019

The [4Fe-4S] Cluster Domain of the Nitrogenase Iron Protein Facilitates Conformational Changes Required for the Cooperative Binding of Two Nucleotides†

scientific article published on 01 December 1996

The flexible N-terminus of BchL autoinhibits activity through interaction with its [4Fe-4S] cluster and relieved upon ATP binding

scientific article published on 20 November 2020

The identification, characterization, sequencing and mutagenesis of the genes (hupSL) encoding the small and large subunits of the H2-uptake hydrogenase of Azotobacter chroococcum

scientific article published in June 1990

The role of the MoFe protein alpha-125Phe and beta-125Phe residues in Azotobacter vinelandii MoFe protein-Fe protein interaction

scientific article published in July 2000

Thermodynamics of nucleotide interactions with the Azotobacter vinelandii nitrogenase iron protein

article

Time-Resolved EPR Study of H2 Reductive Elimination from the Photoexcited Nitrogenase Janus E4(4H) Intermediate

scientific article published on 08 October 2019

Trapping a hydrazine reduction intermediate on the nitrogenase active site

scientific article published in June 2005

Trapping an intermediate of dinitrogen (N2) reduction on nitrogenase

scientific article

Uncoupling nitrogenase: catalytic reduction of hydrazine to ammonia by a MoFe protein in the absence of Fe protein-ATP

scientific article

Understanding precision nitrogen stress to optimize the growth and lipid content tradeoff in oleaginous green microalgae.

scientific article

Unification of reaction pathway and kinetic scheme for N2 reduction catalyzed by nitrogenase

scientific article published on 29 March 2012

List of works by Lance C Seefeldt

57Fe ENDOR spectroscopy and 'electron inventory' analysis of the nitrogenase E4 intermediate suggest the metal-ion core of FeMo-cofactor cycles through only one redox couple

A Continuous, Spectrophotometric Activity Assay for Nitrogenase Using the Reductant Titanium(III) Citrate

A Mediated Thin-Layer Voltammetry Method for the Study of Redox Protein Electrochemistry

A confirmation of the quench-cryoannealing relaxation protocol for identifying reduction states of freeze-trapped nitrogenase intermediates

A conformational mimic of the MgATP-bound "on state" of the nitrogenase iron protein

A substrate channel in the nitrogenase MoFe protein

Aerobic, inactive forms of Azotobacter vinelandii hydrogenase: activation kinetics and insensitivity to C2H2 inhibition

Alkyne substrate interaction within the nitrogenase MoFe protein

An Efficient Viologen-Based Electron Donor to Nitrogenase

Analysis of a gene region required for dihydrogen oxidation in Azotobacter vinelandii

Beyond fossil fuel-driven nitrogen transformations

Biodiesel production by simultaneous extraction and conversion of total lipids from microalgae, cyanobacteria, and wild mixed-cultures

Breaking the N2 triple bond: insights into the nitrogenase mechanism

CO2 Reduction Catalyzed by Nitrogenase: Pathways to Formate, Carbon Monoxide, and Methane

Catalytic and biophysical properties of a nitrogenase Apo-MoFe protein produced by a nifB-deletion mutant of Azotobacter vinelandii

Changes in the Midpoint Potentials of the Nitrogenase Metal Centers as a Result of Iron Protein−Molybdenum-Iron Protein Complex Formation†

Characterization of a Fatty Acyl-CoA Reductase fromMarinobacter aquaeoleiVT8: A Bacterial Enzyme Catalyzing the Reduction of Fatty Acyl-CoA to Fatty Alcohol

Cluster Dependent Charge-Transfer Dynamics in Iron-Sulfur Proteins.

Competitive substrate and inhibitor interactions at the physiologically relevant active site of nitrogenase

Conformational gating of electron transfer from the nitrogenase Fe protein to MoFe protein

Connecting nitrogenase intermediates with the kinetic scheme for N2 reduction by a relaxation protocol and identification of the N2 binding state.

Construction and characterization of a heterodimeric iron protein: defining roles for adenosine triphosphate in nitrogenase catalysis.

Control of electron transfer in nitrogenase

Correction to "Establishing a Thermodynamic Landscape for the Active Site of Mo-Dependent Nitrogenase"

Correction to "High-Resolution ENDOR Spectroscopy Combined with Quantum Chemical Calculations Reveals the Structure of Nitrogenase Janus Intermediate E4(4H)"

Crystal structure of the L protein of Rhodobacter sphaeroides light-independent protochlorophyllide reductase with MgADP bound: a homologue of the nitrogenase Fe protein

Defining Intermediates of Nitrogenase MoFe Protein during N2 Reduction under Photochemical Electron Delivery from CdS Quantum Dots

Determination of rate and equilibrium constants for the reactions between electron transfer mediators and proteins by linear sweep voltammetry.

Diazene (HN=NH) is a substrate for nitrogenase: insights into the pathway of N2 reduction.

ENDOR/HYSCORE studies of the common intermediate trapped during nitrogenase reduction of N2H2, CH3N2H, and N2H4 support an alternating reaction pathway for N2 reduction

EXAFS and NRVS reveal a conformational distortion of the FeMo-cofactor in the MoFe nitrogenase propargyl alcohol complex

Electrocatalytic CO2 reduction catalyzed by nitrogenase MoFe and FeFe proteins.

Electron Paramagnetic Resonance Spectroscopy

Electron Redistribution within the Nitrogenase Active Site FeMo-Cofactor During Reductive Elimination of H<sub>2</sub> to Achieve N≡N Triple-Bond Activation

Electron Transfer from the Nitrogenase Iron Protein to the [8Fe-(7/8)S] Clusters of the Molybdenum−Iron Protein†

Electron Transfer in Nitrogenase Analyzed by Marcus Theory: Evidence for Gating by MgATP†

Electron transfer in nitrogenase catalysis.

Electron transfer precedes ATP hydrolysis during nitrogenase catalysis.

Electron transfer within nitrogenase: evidence for a deficit-spending mechanism

Elucidating the Mechanism of Nucleotide-Dependent Changes in the Redox Potential of the [4Fe-4S] Cluster in Nitrogenase Iron Protein: The Role of Phenylalanine 135†

Energy Transduction in Nitrogenase

Entropies of Redox Reactions between Proteins and Mediators: The Temperature Dependence of Reversible Electrode Potentials in Aqueous Buffers ⬇️

Establishing a Thermodynamic Landscape for the Active Site of Mo-Dependent Nitrogenase

Evidence That MgATP Accelerates Primary Electron Transfer in aClostridium pasteurianumFe Protein-Azotobacter vinelandiiMoFe Protein Nitrogenase Tight Complex

Evidence That the Pi Release Event Is the Rate-Limiting Step in the Nitrogenase Catalytic Cycle

Evidence for Electron Transfer from the Nitrogenase Iron Protein to the Molybdenum−Iron Protein without MgATP Hydrolysis: Characterization of a Tight Protein−Protein Complex†

Evidence for Electron Transfer-dependent Formation of a Nitrogenase Iron Protein-Molybdenum-Iron Protein Tight Complex

Evidence for coupled electron and proton transfer in the [8Fe-7S] cluster of nitrogenase

Exploring Electron/Proton Transfer and Conformational Changes in the Nitrogenase MoFe Protein and FeMo-cofactor Through Cryoreduction/EPR Measurements.

Exploring the alternatives of biological nitrogen fixation.

Fe protein-independent substrate reduction by nitrogenase MoFe protein variants

Frontiers, opportunities, and challenges in biochemical and chemical catalysis of CO2 fixation

High-Resolution ENDOR Spectroscopy Combined with Quantum Chemical Calculations Reveals the Structure of Nitrogenase Janus Intermediate E4(4H)

Hydride Conformers of the Nitrogenase FeMo-cofactor Two-Electron Reduced State E2(2H), Assigned Using Cryogenic Intra Electron Paramagnetic Resonance Cavity Photolysis.

Hydrolysis of Nucleoside Triphosphates Other than ATP by Nitrogenase

Identification of a key catalytic intermediate demonstrates that nitrogenase is activated by the reversible exchange of N₂ for H₂.

Improving energetics of triacylglyceride extraction from wet oleaginous microbes.

Infrared spectroscopy of the nitrogenase MoFe protein under electrochemical control: potential-triggered CO binding.

Insights into nucleotide signal transduction in nitrogenase: structure of an iron protein with MgADP bound

Insights into substrate binding at FeMo-cofactor in nitrogenase from the structure of an alpha-70(Ile) MoFe protein variant

Insights into the role of nucleotide-dependent conformational change in nitrogenase catalysis: Structural characterization of the nitrogenase Fe protein Leu127 deletion variant with bound MgATP

Interaction of acetylene and cyanide with the resting state of nitrogenase alpha-96-substituted MoFe proteins

Intermediates Trapped during Nitrogenase Reduction of N⋮N, CH3−NNH, and H2N−NH2

Isolation and characterization of an acetylene-resistant nitrogenase

Light-driven dinitrogen reduction catalyzed by a CdS:nitrogenase MoFe protein biohybrid

Localization of a catalytic intermediate bound to the FeMo-cofactor of nitrogenase

Localization of a substrate binding site on the FeMo-cofactor in nitrogenase: trapping propargyl alcohol with an alpha-70-substituted MoFe protein

Mechanism of Mo-Dependent Nitrogenase

Mechanism of Nitrogenase H2 Formation by Metal-Hydride Protonation Probed by Mediated Electrocatalysis and H/D Isotope Effects.

Mechanism of nitrogen fixation by nitrogenase: the next stage

Mo-, V-, and Fe-Nitrogenases Use a Universal Eight-Electron Reductive-Elimination Mechanism To Achieve N2 Reduction

Molybdenum nitrogenase catalyzes the reduction and coupling of CO to form hydrocarbons

Negative cooperativity in the nitrogenase Fe protein electron delivery cycle.

Nitrite and hydroxylamine as nitrogenase substrates: mechanistic implications for the pathway of N₂ reduction

Nitrogen fixation: the mechanism of the Mo-dependent nitrogenase.

Nitrogenase Reduction of Carbon Disulfide: Freeze-Quench EPR and ENDOR Evidence for Three Sequential Intermediates with Cluster-Bound Carbon Moieties†

Nitrogenase: a draft mechanism

Oleaginous yeast platform for producing biofuels via co-solvent hydrothermal liquefaction

On reversible H2 loss upon N2 binding to FeMo-cofactor of nitrogenase.