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List of works by Luis M. Rubio

A cyanobacterial narB gene encodes a ferredoxin-dependent nitrate reductase

article

A sterile alpha-motif domain in NafY targets apo-NifDK for iron-molybdenum cofactor delivery via a tethered domain

scientific article

Adaptation of the GoldenBraid modular cloning system and creation of a toolkit for the expression of heterologous proteins in yeast mitochondria.

scientific article published on 13 November 2017

Biosynthesis of Nitrogenase Cofactors

scientific article published on 24 January 2020

Biosynthesis of the nitrogenase active-site cofactor precursor NifB-co in Saccharomyces cerevisiae

scientific article published on 25 November 2019

Challenges to develop nitrogen-fixing cereals by direct nif-gene transfer.

scientific article published on 11 June 2014

Cloning and mutational analysis of the gamma gene from Azotobacter vinelandii defines a new family of proteins capable of metallocluster binding and protein stabilization.

scientific article

Corrigendum to "Challenges to develop nitrogen-fixing cereals by direct nif-gene transfer" [Plant Sci. 225 (August) (2014) 130-137]

scientific article published on 25 April 2017

Diversity and Functional Analysis of the FeMo-Cofactor Maturase NifB.

scientific article published on 14 November 2017

EXAFS reveals two Mo environments in the nitrogenase iron-molybdenum cofactor biosynthetic protein NifQ.

scientific article published on September 2016

Electron Paramagnetic Resonance Characterization of Three Iron-Sulfur Clusters Present in the Nitrogenase Cofactor Maturase NifB from Methanocaldococcus infernus

scientific article published on 6 June 2016

Enzyme-catalysed nitrate reduction-themes and variations as revealed by protein film voltammetry

scientific article published in May 2002

Evidence for nifU and nifS participation in the biosynthesis of the iron-molybdenum cofactor of nitrogenase.

scientific article published on 24 October 2007

Expression and purification of NifB proteins from aerobic and anaerobic sources.

scientific article published in January 2014

Expression of a functional oxygen-labile nitrogenase component in the mitochondrial matrix of aerobically grown yeast.

scientific article published on 29 April 2016

Extended X-ray absorption fine structure and nuclear resonance vibrational spectroscopy reveal that NifB-co, a FeMo-co precursor, comprises a 6Fe core with an interstitial light atom.

scientific article published on 02 April 2008

Formation of Nitrogenase NifDK Tetramers in the Mitochondria of Saccharomyces cerevisiae.

scientific article

Genes required for rapid expression of nitrogenase activity in Azotobacter vinelandii

scientific article

Genome sequence of Azotobacter vinelandii, an obligate aerobe specialized to support diverse anaerobic metabolic processes

scientific article published on 08 May 2009

Hydrogen overproducing nitrogenases obtained by random mutagenesis and high-throughput screening.

scientific article

Identification of a Mo-Fe-S cluster on NifEN by Mo K-edge extended X-ray absorption fine structure.

scientific article published on 22 February 2007

In vitro synthesis of the iron-molybdenum cofactor of nitrogenase from iron, sulfur, molybdenum, and homocitrate using purified proteins

scholarly article

Kinetics of Nif gene expression in a nitrogen-fixing bacterium

scientific article published on 15 November 2013

Maturation of nitrogenase: a biochemical puzzle

scientific article

Molybdenum trafficking for nitrogen fixation

scientific article

Molybdopterin guanine dinucleotide cofactor in Synechococcus sp. nitrate reductase: identification of mobA and isolation of a putative moeB gene

scientific article published in December 1999

NAD-, NMN-, and NADP-dependent modification of dinitrogenase reductases from Rhodospirillum rubrum and Azotobacter vinelandii

scientific article

New insights into the mechanism of nickel insertion into carbon monoxide dehydrogenase: analysis of Rhodospirillum rubrum carbon monoxide dehydrogenase variants with substituted ligands to the [Fe3S4] portion of the active-site C-cluster

scientific article

NifB and NifEN protein levels are regulated by ClpX2 under nitrogen fixation conditions in Azotobacter vinelandii.

scientific article

NifX and NifEN exchange NifB cofactor and the VK-cluster, a newly isolated intermediate of the iron-molybdenum cofactor biosynthetic pathway

scientific article

Purification and In Vitro Activity of Mitochondria Targeted Nitrogenase Cofactor Maturase NifB

scientific article published on 12 September 2017

Purification of O2-sensitive metalloproteins

scientific article published on 01 January 2014

Purification of a NifEN protein complex that contains bound molybdenum and a FeMo-Co precursor from an Azotobacter vinelandii DeltanifHDK strain

scientific article published in October 2006

Purification, cofactor analysis, and site-directed mutagenesis of Synechococcus ferredoxin-nitrate reductase.

scientific article

Role of Azotobacter vinelandii FdxN in FeMo-co biosynthesis.

scientific article

Roles of Four Conserved Basic Amino Acids in a Ferredoxin-Dependent Cyanobacterial Nitrate Reductase ⬇️

scientific article published on June 13, 2013

State of the Art in Eukaryotic Nitrogenase Engineering.

scientific article

Substrate specificity and evolutionary implications of a NifDK enzyme carrying NifB-co at its active site

scientific article published on 26 February 2010

The Nitrogenase FeMo-Cofactor Precursor Formed by NifB Protein: A Diamagnetic Cluster Containing Eight Iron Atoms

scientific article published on 9 September 2016

The Nitrogenase FeMo-Cofactor Precursor Formed by NifB Protein: A Diamagnetic Cluster Containing Eight Iron Atoms

The narA Locus of Synechococcus sp. Strain PCC 7942 Consists of a Cluster of Molybdopterin Biosynthesis Genes ⬇️

scientific article published on March 1, 1998

Tuning a nitrate reductase for function. The first spectropotentiometric characterization of a bacterial assimilatory nitrate reductase reveals novel redox properties.

scientific article published on 27 May 2004

Use of synthetic biology tools to optimize the production of active nitrogenase Fe protein in chloroplasts of tobacco leaf cells

scientific article published on 27 January 2020

VnfY is required for full activity of the vanadium-containing dinitrogenase in Azotobacter vinelandii

scientific article

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