List of works by Guido Grandi

125Single Nucleotide Polymorphisms Drive Phenotypic Diversity Among Sequence Type 1 Group B Streptococcus, An Emerging Cause of Invasive Disease in Adult Humans

scientific article published in December 2014

A Bacillus subtilis large ORF coding for a polypeptide highly similar to polyketide synthases

scientific article published on August 1993

A chemokine-degrading extracellular protease made by group A Streptococcus alters pathogenesis by enhancing evasion of the innate immune response

scientific article

A new flow-cytometry-based opsonophagocytosis assay for the rapid measurement of functional antibody levels against Group B Streptococcus.

scientific article published on 30 January 2012

A new human growth hormone production process using a recombinant Bacillus subtilis strain

article

A putative new peptide synthase operon in Bacillus subtilis: partial characterization.

scientific article

A rapid and versatile site-directed method of mutagenesis for double-stranded plasmid DNA.

scientific article published in September 1988

An operon encoding a novel ABC-type transport system in Bacillus subtilis

scientific article

Analysis of two-component systems in group B Streptococcus shows that RgfAC and the novel FspSR modulate virulence and bacterial fitness.

scientific article

Antibody-mediated immunity induced by engineered Escherichia coli OMVs carrying heterologous antigens in their lumen

scientific article

Approach to discover T- and B-cell antigens of intracellular pathogens applied to the design of Chlamydia trachomatis vaccines

scientific article

Auto-Assembling Detoxified Staphylococcus aureus Alpha-Hemolysin Mimicking the Wild-Type Cytolytic Toxin

scientific article published on 30 March 2016

Bacterial surface proteins and vaccines ⬇️

scientific article published on May 11, 2010

Bacterial vaccine discovery: from "brute force" to high selectivity

scientific article published in November 2010

BibA Induces Opsonizing Antibodies Conferring In Vivo Protection against Group BStreptococcus

article

BibA: a novel immunogenic bacterial adhesin contributing to group B Streptococcus survival in human blood

article

CD81 extracellular domain 3D structure: insight into the tetraspanin superfamily structural motifs.

scientific article

CT043, a protective antigen that induces a CD4+ Th1 response during Chlamydia trachomatis infection in mice and humans.

scientific article published on 13 July 2009

Capturing host-pathogen interactions by protein microarrays: identification of novel streptococcal proteins binding to human fibronectin, fibrinogen, and C4BP.

scientific article published on 5 May 2009

Characterization and identification of vaccine candidate proteins through analysis of the group A Streptococcus surface proteome.

scientific article published on 15 January 2006

Characterization of a novel Neisseria meningitidis Fur and iron-regulated operon required for protection from oxidative stress: utility of DNA microarray in the assignment of the biological role of hypothetical genes.

scientific article

Characterization of the locus encoding the [Ni-Fe] sulfhydrogenase from the archaeon Pyrococcus furiosus: evidence for a relationship to bacterial sulfite reductases

scientific article published in February 1995

Characterization of the srfA locus of Bacillus subtilis: only the valine-activating domain of srfA is involved in the establishment of genetic competence

scientific article published on May 1993

Characterization of the surfactin synthetase multi-enzyme complex

scientific article published on 01 March 1994

Chlamydia pneumoniae genome sequence analysis and identification of HLA-A2-restricted CD8+ T cell epitopes recognized by infection-primed T cells.

scientific article published on October 2005

Combined conjugate vaccines: enhanced immunogenicity with the N19 polyepitope as a carrier protein

scientific article

Comparative genomics of Neisseria meningitidis: core genome, islands of horizontal transfer and pathogen-specific genes

article

Complete Genome Sequence of Neisseria meningitidis Serogroup B Strain MC58

scientific article

Complete genome sequence and comparative genomic analysis of an emerging human pathogen, serotype V Streptococcus agalactiae

scientific article

Coordinate transcription and physical linkage of domains in surfactin synthetase are not essential for proper assembly and activity of the multienzyme complex

scientific article published in June 1998

Crystal structures of the components of the Staphylococcus aureus leukotoxin ED.

scientific article published on January 2016

Crystallization and preliminary crystallographic studies on the large extracellular domain of human CD81, a tetraspanin receptor for hepatitis C virus.

scientific article published in January 2001

CsrRS regulates group B Streptococcus virulence gene expression in response to environmental pH: a new perspective on vaccine development

scientific article published on 19 June 2009

Cumulative stabilizing effects of glycine to alanine substitutions in Bacillus subtilis neutral protease

scientific article published on September 1992

Cumulative stabilizing effects of hydrophobic interactions on the surface of the neutral protease from Bacillus subtilis

scientific article published in May 1996

Different profile of CD8+ effector T cells induced in Der p 1-allergic and naïve mice by DNA vaccination

scientific article

Different specific activities of the monomeric and oligomeric forms of plasmid DNA in transformation of B. subtilis and E. coli ⬇️

scientific article published on July 24, 1979

Discovery of a vaccine antigen that protects mice from Chlamydia pneumoniae infection.

scientific article published on 13 December 2006

Effect of Glu-143 and His-231 substitutions on the catalytic activity and secretion of Bacillus subtilis neutral protease

scientific article published on 01 January 1989

Efficient conversion of 5-substituted hydantoins to D-alpha-amino acids using recombinant Escherichia coli strains

scientific article published in April 1998

Environmental acidification drives S. pyogenes pilus expression and microcolony formation on epithelial cells in a FCT-dependent manner

scientific article (publication date: 5 November 2010)

Evaluation of a Group A Streptococcus synthetic oligosaccharide as vaccine candidate

scientific article published on 24 September 2010

Experimental infection by Chlamydia pneumoniae in the hamster

scientific article published on March 2004

Exploiting Antigenic Diversity for Vaccine Design: THE CHLAMYDIA ArtJ PARADIGM

scientific article

Expression of Bordetella pertussis fimbrial (fim) genes in Bordetella bronchiseptica: fimX is expressed at a low level and vir-regulated

scientific article published in May 1991

Four-component Staphylococcus aureus vaccine 4C-staph enhances Fcγ receptor expression in neutrophils and monocytes and mitigates S. aureus infection in neutropenic mice

scientific article

Functional activity of maternal and cord antibodies elicited by an investigational group B streptococcus trivalent glycoconjugate vaccine in pregnant women

scientific article published on 25 January 2018

Functional characterization of a newly identified group B Streptococcus pullulanase eliciting antibodies able to prevent alpha-glucans degradation

scientific article

GNA33 from Neisseria meningitidis serogroup B encodes a membrane-bound lytic transglycosylase (MltA).

scientific article published in August 2002

GNA33 of Neisseria meningitidis is a lipoprotein required for cell separation, membrane architecture, and virulence.

scientific article

Gene expression profile in Neisseria meningitidis and Neisseria lactamica upon host-cell contact: from basic research to vaccine development.

scientific article published on December 2002

Genome Analysis Reveals Pili in Group B Streptococcus

scientific article published in Science

Genome analysis of multiple pathogenic isolates of Streptococcus agalactiae: implications for the microbial "pan-genome"

scientific article

Genomic analysis reveals the molecular basis for capsule loss in the group B Streptococcus population

scientific article

Genomic approach for analysis of surface proteins in Chlamydia pneumoniae

scientific article

Group A Streptococcus produce pilus-like structures containing protective antigens and Lancefield T antigens

scientific article

Group B Streptococcus Pullulanase Crystal Structures in the Context of a Novel Strategy for Vaccine Development

scientific article

Group B Streptococcus crosses human epithelial cells by a paracellular route

scientific article published on 9 December 2005

Group B Streptococcus: global incidence and vaccine development

scientific article published on 6 November 2006

Helicobacter pyloritoxin VacA induces vacuole formation by acting in the cell cytosol

scientific article published on 01 November 1997

Identification of 2 hypothetical genes involved in Neisseria meningitidis cathelicidin resistance.

scientific article

Identification of a universal Group B streptococcus vaccine by multiple genome screen

scientific article

Identification of iron-activated and -repressed Fur-dependent genes by transcriptome analysis of Neisseria meningitidis group B.

scientific article

Identification of new potential vaccine candidates against Chlamydia pneumoniae by multiple screenings.

scientific article published on January 2005

Identification of novel genomic islands coding for antigenic pilus-like structures in Streptococcus agalactiae.

scientific article

Identification of vaccine candidates against serogroup B meningococcus by whole-genome sequencing.

scientific article

Immobilized metal-ion affinity chromatography of human growth hormone

scientific article published on 01 June 1989

In vitro transcription and translation coupled to two-dimensional electrophoresis for bacterial proteome analysis.

scientific article published on January 2007

Inferring reasons for the failure of Staphylococcus aureus vaccines in clinical trials

scientific article published on 22 February 2012

Inhibitory activity of IL-1 receptor antagonist depends on the balance between binding capacity for IL-1 receptor type 1 and IL-1 receptor type II.

scientific article published on April 1997

Introduction of unmarked mutations in the Helicobacter pylori vacA gene with a sucrose sensitivity marker

scientific article

MF59- and Al(OH)3-Adjuvanted Staphylococcus aureus (4C-Staph) Vaccines Induce Sustained Protective Humoral and Cellular Immune Responses, with a Critical Role for Effector CD4 T Cells at Low Antibody Titers

scientific article

Mapping of receptor binding sites on IL-1 beta by reconstruction of IL-1ra-like domains

scientific article published on 01 November 1995

Mg2+ signalling defines the group A streptococcal CsrRS (CovRS) regulon

article

Mining the bacterial unknown proteome: identification and characterization of a novel family of highly conserved protective antigens in Staphylococcus aureus

scientific article

Model building of a thermolysin-like protease by mutagenesis

scientific article published on 01 March 1997

Multi high-throughput approach for highly selective identification of vaccine candidates: the Group A Streptococcus case.

scientific article

Multi-plasmid DNA vaccination avoids antigenic competition and enhances immunogenicity of a poorly immunogenic plasmid

article

N19 polyepitope as a carrier for enhanced immunogenicity and protective efficacy of meningococcal conjugate vaccines

scientific article

New insights into the role of the glutamic acid of the E-box motif in group B Streptococcus pilus 2a assembly

article

New plasmid expression vectors for Bacillus subtilis

scientific article published on 01 July 1986

One Dose of Staphylococcus aureus 4C-Staph Vaccine Formulated with a Novel TLR7-Dependent Adjuvant Rapidly Protects Mice through Antibodies, Effector CD4+ T Cells, and IL-17A.

scientific article published on 26 January 2016

Outer membrane vesicles from group B Neisseria meningitidis delta gna33 mutant: proteomic and immunological comparison with detergent-derived outer membrane vesicles.

scientific article

Phagocyte subsets and lymphocyte clonal deletion behind ineffective immune response to Staphylococcus aureus

scientific article published on 19 May 2015

Pili in gram-positive pathogens

scientific article (publication date: July 2006)

Pilus Backbone Contributes to Group BStreptococcusParacellular Translocation through Epithelial Cells

article

Posttranscriptional regulation of an erythromycin resistance protein specified by plasmic pE194 ⬇️

scientific article published on July 1, 1980

Preventing bacterial infections with pilus-based vaccines: the group B streptococcus paradigm

scientific article published in January 2009

Previously unrecognized vaccine candidates against group B meningococcus identified by DNA microarrays

scientific article published on 12 August 2002

Prochymosin expression in Bacillus subtilis

scientific article published on 01 January 1991

Production processes of recombinant IL-1 beta from Bacillus subtilis: comparison between intracellular and exocellular expression.

scientific article published in May 1991

Protective effect of Group B Streptococcus type-III polysaccharide conjugates against maternal colonization, ascending infection and neonatal transmission in rodent models

scientific article published on 7 February 2018

Protectome analysis: a new selective bioinformatics tool for bacterial vaccine candidate discovery

scientific article

Protein array profiling of tic patient sera reveals a broad range and enhanced immune response against Group A Streptococcus antigens

scientific article

Proteome analysis of Neisseria meningitidis serogroup A.

scientific article

Proteomics characterization of outer membrane vesicles from the extraintestinal pathogenic Escherichia coli DeltatolR IHE3034 mutant.

scientific article published on 2 November 2007

Purification of recombinant human growth hormone by isoelectric focusing in a multicompartment electrolyzer with Immobiline membranes.

scientific article published in September 1992

R-factor-mediated suppression of the galactose-sensitive phenotype of Escherichia coli K-12 galE mutants

scientific article published on 01 July 1981

Rationally designed strings of promiscuous CD4+ T cell epitopes provide help toHaemophilus influenzae type b oligosaccharide: a model for new conjugate vaccines

article

Recombinant and Truncated Tetanus Neurotoxin Light Chain: Cloning, Expression, Purification, and Proteolytic Activity

Recombinant outer membrane vesicles carrying Chlamydia muridarum HtrA induce antibodies that neutralize chlamydial infection in vitro

scientific article published on 06 May 2013

Role of FNR and FNR-regulated, sugar fermentation genes in Neisseria meningitidis infection

scientific article

Scavenger receptor gp340 aggregates group A streptococci by binding pili.

scientific article

Sequence Variation in Group AStreptococcusPili and Association of Pilus Backbone Types with Lancefield T Serotypes

article

Sequence and analysis of the genetic locus responsible for surfactin synthesis in Bacillus subtilis

scientific article published in May 1993

Sequence type 1 group B Streptococcus, an emerging cause of invasive disease in adults, evolves by small genetic changes

scientific article published on 4 May 2015

Simple sequence repeats and genome plasticity in Streptococcus agalactiae

scientific article published on 21 May 2010

Sortase A substrate specificity in GBS pilus 2a cell wall anchoring

scientific article (publication date: 2011)

Sortase A utilizes an ancillary protein anchor for efficient cell wall anchoring of pili in Streptococcus agalactiae

scientific article

Specific involvement of pilus type 2a in biofilm formation in group B Streptococcus

scientific article

Specific pattern of instability of Escherichia coli HisG gene cloned in Bacillus subtilis via the Staphylococcus aureus plasmid pCS194

article

SpyAD, a moonlighting protein of group A Streptococcus contributing to bacterial division and host cell adhesion.

scientific article

Staphylococcus aureus FhuD2 is involved in the early phase of staphylococcal dissemination and generates protective immunity in mice.

scientific article published on 24 July 2012

Streptococcus agalactiae capsule polymer length and attachment is determined by the proteins CpsABCD.

scientific article

Streptococcus pyogenes SpyCEP influences host-pathogen interactions during infection in a murine air pouch model

scientific article (publication date: 2012)

Streptococcus pyogenes SpyCEP: a chemokine-inactivating protease with unique structural and biochemical features.

scientific article published on 25 March 2010

Streptococcus pyogenes pili promote pharyngeal cell adhesion and biofilm formation

scientific article

Structural Basis for Group B Streptococcus Pilus 1 Sortases C Regulation and Specificity

scientific article

Structural and functional characterization of the Staphylococcus aureus virulence factor and vaccine candidate FhuD2

scientific article

Structural features of neutral protease from Bacillus subtilis deduced from model-building and limited proteolysis experiments.

scientific article published in April 1990

Structural vaccinology starts to deliver

scientific article published in 2012

Structure analysis and site-directed mutagenesis of defined key residues and motives for pilus-related sortase C1 in group B Streptococcus

scientific article

Structure and protective efficacy of the Staphylococcus aureus autocleaving protease EpiP.

scientific article published on 13 January 2014

Structure of the type IX group B Streptococcus capsular polysaccharide and its evolutionary relationship with types V and VII

scientific article

Structure, dynamics and immunogenicity of a catalytically inactive CXC chemokine-degrading protease SpyCEP from Streptococcus pyogenes

scientific article published on 13 March 2020

Structure-based approach to rationally design a chimeric protein for an effective vaccine against Group B Streptococcus infections

scientific article

Subunit association and conformational flexibility in the head subdomain of human CD81 large extracellular loop

scientific article

Surface interactome in Streptococcus pyogenes

scientific article published on 22 December 2011

Surfome analysis as a fast track to vaccine discovery: identification of a novel protective antigen for Group B Streptococcus hypervirulent strain COH1.

scientific article published on 28 April 2009

Synergistic Protective Activity of Tumor-Specific Epitopes Engineered in Bacterial Outer Membrane Vesicles

scientific article published on 7 November 2017

Targeted amino acid substitutions impair streptolysin O toxicity and group A Streptococcus virulence

scientific article published on 8 January 2013

The Helicobacter pylori neutrophil-activating protein is an iron-binding protein with dodecameric structure.

scientific article published in October 1999

The Human Pathogen Streptococcus pyogenes Releases Lipoproteins as Lipoprotein-rich Membrane Vesicles

scientific article published on 27 May 2015

The Protective Value of Maternal Group B Streptococcus Antibodies: Quantitative and Functional Analysis of Naturally Acquired Responses to Capsular Polysaccharides and Pilus Proteins in European Maternal Sera

scientific article published on 11 July 2016

The [Ni-Fe] hydrogenase from the thermophilic bacterium Acetomicrobium flavidum

scientific article published in April 1996

The ancillary protein 1 of Streptococcus pyogenes FCT-1 pili mediates cell adhesion and biofilm formation through heterophilic as well as homophilic interactions

scientific article published on 10 February 2012

The complete genome sequence of the gram-positive bacterium Bacillus subtilis

scientific article

The effect of engineering surface loops on the thermal stability of Bacillus subtilis neutral protease

scientific article published on 01 March 1994

The impact of genomics in vaccine discovery: achievements and lessons

article

Three-Dimensional Human Skin Models to Understand Staphylococcus aureus Skin Colonization and Infection

scientific article published on 06 February 2014

Topological Mapping of the Cysteine Residues ofN-Carbamyl-D-amino-acid Amidohydrolase and Their Role in Enzymatic Activity

scientific article published in Journal of Biological Chemistry

Transcriptional and proteomic profiles of group B Streptococcus type V reveal potential adherence proteins associated with high-level invasion

scientific article published on 8 January 2007

Transfection of E. coli with lambda DNA by electroporation

scientific article published in October 1992

Translational attenuation of ermC: a deletion analysis

scientific article

Typing of the pilus-protein-encoding FCT region and biofilm formation as novel parameters in epidemiological investigations of Streptococcus pyogenes isolates from various infection sites

scientific article published on 10 December 2009

Understanding the molecular determinants driving the immunological specificity of the protective pilus 2a backbone protein of group B streptococcus

scientific article

Use ofLactococcus lactisExpressing Pili from Group BStreptococcusas a Broad‐Coverage Vaccine against Streptococcal Disease

article

Vaccine composition formulated with a novel TLR7-dependent adjuvant induces high and broad protection against Staphylococcus aureus

scientific article

Why is a protective antigen protective?

scientific article published on December 2009

nprR1 and nprR2 regulatory regions for neutral protease expression in Bacillus subtilis.

scientific article

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