List of works - Gabriel A. Monteiro

A Quantitative ELISA for Monitoring the Secretion of ZZ-Fusion Proteins Using SpA Domain as Immunodetection Reporter System

scientific article published on 01 November 2001

A quantitative method to evaluate mesenchymal stem cell lipofection using real-time PCR.

scientific article

Analysis and use of endogenous nuclease activities in Escherichia coli lysates during the primary isolation of plasmids for gene therapy

scientific article published in January 1999

Analysis of DNA repeats in bacterial plasmids reveals the potential for recurrent instability events

scientific article published on 23 May 2010

Application of central composite design for DNA hybridization onto magnetic microparticles

scientific article published on 10 May 2009

Characterization of the Topography and Wettability of English Weed Leaves and Biomimetic Replicas

Chemiluminescent bead-based hybridization assay for the detection of genomic DNA from E. coli in purified plasmid samples.

scientific article published on 15 May 2008

Comparative analysis of antigen-targeting sequences used in DNA vaccines.

scientific article published in March 2010

Comparison of real-time polymerase chain reaction and hybridization assays for the detection of Escherichia coli genomic DNA in process samples and pharmaceutical-grade plasmid DNA products.

scientific article

Conjugal transfer of recombinant plasmids into gellan gum-producing and non-producing variants of Pseudomonas elodea ATCC 31461.

scientific article

DNA Vaccines Against Maedi-Visna Virus.

scientific article published on January 2016

DNA vaccines: a rational design against parasitic diseases.

scientific article published on February 2010

De novo creation of MG1655-derived E. coli strains specifically designed for plasmid DNA production.

scientific article published on 12 August 2012

Deletion formation mutations in plasmid expression vectors are unfavored by runaway amplification conditions and differentially selected under kanamycin stress

scientific article published on 7 August 2009

Design and characterization of plasmids encoding antigenic peptides of Aha1 from Aeromonas hydrophila as prospective fish vaccines.

scientific article

Development of a candidate DNA vaccine against Maedi-Visna virus.

scientific article

Development of a nicking endonuclease-assisted method for the purification of minicircles.

scientific article

Development of a recombinant fusion protein based on the dynein light chain LC8 for non-viral gene delivery

scientific article published on 20 January 2012

Draft Genome Sequence of the Plasmid-Free Lactococcus lactis subsp. lactis Strain LMG 19460

scientific article published on 20 April 2017

Effect of cationic liposomes/DNA charge ratio on gene expression and antibody response of a candidate DNA vaccine against Maedi Visna virus.

scientific article published on 14 May 2009

Engineering of Escherichia coli strains for plasmid biopharmaceutical production: scale-up challenges

scientific article published on 2 March 2014

Engineering of Human Mesenchymal Stem/Stromal Cells with Vascular Endothelial Growth Factor-Encoding Minicircles for Angiogenic Ex Vivo Gene Therapy

scientific article published on 13 November 2018

Enhancement of DNA vaccine efficacy by intracellular targeting strategies

scientific article published on January 2014

Evaluation of bottlenecks in proinsulin secretion by Escherichia coli.

scientific article published in April 2004

Evaluation of inducible promoters on the secretion of a ZZ-proinsulin fusion protein in Escherichia coli.

scientific article

Evaluation of the effect of non-B DNA structures on plasmid integrity via accelerated stability studies.

scientific article published on April 2009

Evidence for the in vivo expression of a distant downstream gene under the control of ColE1 replication origin.

scientific article

Evidence that the insertion events of IS2 transposition are biased towards abrupt compositional shifts in target DNA and modulated by a diverse set of culture parameters

scientific article published on 27 April 2014

Fluorometric determination of ethidium bromide efflux kinetics in Escherichia coli

scientific article

High frequency plasmid recombination mediated by 28 bp direct repeats

scientific article published on 8 July 2008

Impact of plasmid quality on lipoplex-mediated transfection

scientific article

Improvement of DNA minicircle production by optimization of the secondary structure of the 5'-UTR of ParA resolvase

scientific article

In vivo activation of yeast plasma membrane H+-ATPase by ethanol: effect on the kinetic parameters and involvement of the carboxyl-terminus regulatory domain

scientific article published on March 1998

Isolation of plasmid DNA from cell lysates by aqueous two-phase systems

scientific article

Medium and copy number effects on the secretion of human proinsulin in Escherichia coli using the universal stress promoters uspA and uspB.

scientific article

Monitoring intracellular calcium in response to GPCR activation using thin-film silicon photodiodes with integrated fluorescence filters

scientific article published on 5 September 2013

Mutation detection in plasmid-based biopharmaceuticals.

scientific article published on 16 February 2011

ORMOPLEXEs for gene therapy: In vitro and in vivo assays.

scientific article published on 5 March 2016

On the dual effect of glucose during production of pBAD/AraC-based minicircles.

scientific article

On the stability of plasmid DNA vectors during cell culture and purification.

scientific article published in June 2007

Optimization of the primary recovery of human interferon alpha2b from Escherichia coli inclusion bodies.

scientific article

Plasmid Biopharmaceuticals

scientific article published on December 2014

Plasmid Copy Number of pTRKH3 in Lactococcus lactis is Increased by Modification of the repDE Ribosome-Binding Site

scientific article published on 20 May 2019

Plasmid DNA production with Escherichia coli GALG20, a pgi-gene knockout strain: fermentation strategies and impact on downstream processing

scientific article published in July 2014

Plasmid DNA size does affect nonviral gene delivery efficiency in stem cells

scientific article published on 17 February 2012

Production and Purification of Supercoiled Minicircles by a Combination of In Vitro Endonuclease Nicking and Hydrophobic Interaction Chromatography

scientific article published on 10 August 2018

Production, purification and analysis of an experimental DNA vaccine against rabies.

scientific article published in November 2001

Protein-DNA interactions define the mechanistic aspects of circle formation and insertion reactions in IS2 transposition.

scientific article published on 26 January 2012

Purification of a cystic fibrosis plasmid vector for gene therapy using hydrophobic interaction chromatography

scientific article published in June 2000

Purification of plasmids for gene therapy and DNA vaccination.

scientific article

Quantitation of non-amplified genomic DNA by bead-based hybridization and template mediated extension coupled to alkaline phosphatase signal amplification

scientific article published on 17 October 2009

Quantitative evaluation of DNA dissociation from liposome carriers and DNA escape from endosomes during lipid-mediated gene delivery.

scientific article published on October 2014

Rational engineering of Escherichia coli strains for plasmid biopharmaceutical manufacturing

scientific article published on 13 September 2011

Re-engineering of an Escherichia coli K-12 strain for the efficient production of recombinant human Interferon Gamma

scientific article published on 07 June 2018

Recombination frequency in plasmid DNA containing direct repeats--predictive correlation with repeat and intervening sequence length

scientific article

Separation and Analysis of Plasmid Denatured Forms Using Hydrophobic Interaction Chromatography

scientific article published on 01 November 1999

Stabilization of naked and condensed plasmid DNA against degradation induced by ultrasounds and high-shear vortices

scientific article published on 22 June 2009

Structural instability of plasmid biopharmaceuticals: challenges and implications.

scientific article published on 03 August 2009

The Influence of Nutrient Medium Composition on Escherichia coli Biofilm Development and Heterologous Protein Expression

scientific article published in 2021

The impact of polyadenylation signals on plasmid nuclease-resistance and transgene expression

scientific article published on May 2007

The role of polyadenylation signal secondary structures on the resistance of plasmid vectors to nucleases.

scientific article published in May 2004

The role of probe–probe interactions on the hybridization of double-stranded DNA targets onto DNA-modified magnetic microparticles

scientific article published on 22 May 2009

Time-course determination of plasmid content in eukaryotic and prokaryotic cells using real-time PCR.

scientific article published in October 2007

Towards effective non-viral gene delivery vector.

scientific article published on April 2015

Towards the miniaturization of GPCR-based live-cell screening assays.

scientific article published on 23 August 2012

Trans-sialidase from Trypanosoma brucei as a potential target for DNA vaccine development against African trypanosomiasis

scientific article published on 7 July 2009

Translational features of human alpha 2b interferon production in Escherichia coli.

scientific article

Troubleshooting in Gene Splicing by Overlap Extension: A Step-Wise Method

article

Trypanosoma brucei: immunisation with plasmid DNA encoding invariant surface glycoprotein gene is able to induce partial protection in experimental African trypanosomiasis.

scientific article published on 18 June 2010

List of works by Gabriel A. Monteiro

A Quantitative ELISA for Monitoring the Secretion of ZZ-Fusion Proteins Using SpA Domain as Immunodetection Reporter System

A quantitative method to evaluate mesenchymal stem cell lipofection using real-time PCR.

Analysis and use of endogenous nuclease activities in Escherichia coli lysates during the primary isolation of plasmids for gene therapy

Analysis of DNA repeats in bacterial plasmids reveals the potential for recurrent instability events

Application of central composite design for DNA hybridization onto magnetic microparticles

Characterization of the Topography and Wettability of English Weed Leaves and Biomimetic Replicas

Chemiluminescent bead-based hybridization assay for the detection of genomic DNA from E. coli in purified plasmid samples.

Comparative analysis of antigen-targeting sequences used in DNA vaccines.

Comparison of real-time polymerase chain reaction and hybridization assays for the detection of Escherichia coli genomic DNA in process samples and pharmaceutical-grade plasmid DNA products.

Conjugal transfer of recombinant plasmids into gellan gum-producing and non-producing variants of Pseudomonas elodea ATCC 31461.

DNA Vaccines Against Maedi-Visna Virus.

DNA vaccines: a rational design against parasitic diseases.

De novo creation of MG1655-derived E. coli strains specifically designed for plasmid DNA production.

Deletion formation mutations in plasmid expression vectors are unfavored by runaway amplification conditions and differentially selected under kanamycin stress

Design and characterization of plasmids encoding antigenic peptides of Aha1 from Aeromonas hydrophila as prospective fish vaccines.

Development of a candidate DNA vaccine against Maedi-Visna virus.

Development of a nicking endonuclease-assisted method for the purification of minicircles.

Development of a recombinant fusion protein based on the dynein light chain LC8 for non-viral gene delivery

Draft Genome Sequence of the Plasmid-Free Lactococcus lactis subsp. lactis Strain LMG 19460

Effect of cationic liposomes/DNA charge ratio on gene expression and antibody response of a candidate DNA vaccine against Maedi Visna virus.

Engineering of Escherichia coli strains for plasmid biopharmaceutical production: scale-up challenges

Engineering of Human Mesenchymal Stem/Stromal Cells with Vascular Endothelial Growth Factor-Encoding Minicircles for Angiogenic Ex Vivo Gene Therapy

Enhancement of DNA vaccine efficacy by intracellular targeting strategies

Evaluation of bottlenecks in proinsulin secretion by Escherichia coli.

Evaluation of inducible promoters on the secretion of a ZZ-proinsulin fusion protein in Escherichia coli.

Evaluation of the effect of non-B DNA structures on plasmid integrity via accelerated stability studies.

Evidence for the in vivo expression of a distant downstream gene under the control of ColE1 replication origin.

Evidence that the insertion events of IS2 transposition are biased towards abrupt compositional shifts in target DNA and modulated by a diverse set of culture parameters

Fluorometric determination of ethidium bromide efflux kinetics in Escherichia coli

High frequency plasmid recombination mediated by 28 bp direct repeats

Impact of plasmid quality on lipoplex-mediated transfection

Improvement of DNA minicircle production by optimization of the secondary structure of the 5'-UTR of ParA resolvase

In vivo activation of yeast plasma membrane H+-ATPase by ethanol: effect on the kinetic parameters and involvement of the carboxyl-terminus regulatory domain

Isolation of plasmid DNA from cell lysates by aqueous two-phase systems

Medium and copy number effects on the secretion of human proinsulin in Escherichia coli using the universal stress promoters uspA and uspB.

Monitoring intracellular calcium in response to GPCR activation using thin-film silicon photodiodes with integrated fluorescence filters

Mutation detection in plasmid-based biopharmaceuticals.

ORMOPLEXEs for gene therapy: In vitro and in vivo assays.

On the dual effect of glucose during production of pBAD/AraC-based minicircles.

On the stability of plasmid DNA vectors during cell culture and purification.

Optimization of the primary recovery of human interferon alpha2b from Escherichia coli inclusion bodies.

Plasmid Biopharmaceuticals

Plasmid Copy Number of pTRKH3 in Lactococcus lactis is Increased by Modification of the repDE Ribosome-Binding Site

Plasmid DNA production with Escherichia coli GALG20, a pgi-gene knockout strain: fermentation strategies and impact on downstream processing

Plasmid DNA size does affect nonviral gene delivery efficiency in stem cells

Production and Purification of Supercoiled Minicircles by a Combination of In Vitro Endonuclease Nicking and Hydrophobic Interaction Chromatography

Production, purification and analysis of an experimental DNA vaccine against rabies.

Protein-DNA interactions define the mechanistic aspects of circle formation and insertion reactions in IS2 transposition.

Purification of a cystic fibrosis plasmid vector for gene therapy using hydrophobic interaction chromatography

Purification of plasmids for gene therapy and DNA vaccination.

Quantitation of non-amplified genomic DNA by bead-based hybridization and template mediated extension coupled to alkaline phosphatase signal amplification

Quantitative evaluation of DNA dissociation from liposome carriers and DNA escape from endosomes during lipid-mediated gene delivery.

Rational engineering of Escherichia coli strains for plasmid biopharmaceutical manufacturing

Re-engineering of an Escherichia coli K-12 strain for the efficient production of recombinant human Interferon Gamma

Recombination frequency in plasmid DNA containing direct repeats--predictive correlation with repeat and intervening sequence length

Separation and Analysis of Plasmid Denatured Forms Using Hydrophobic Interaction Chromatography

Stabilization of naked and condensed plasmid DNA against degradation induced by ultrasounds and high-shear vortices

Structural instability of plasmid biopharmaceuticals: challenges and implications.

The Influence of Nutrient Medium Composition on Escherichia coli Biofilm Development and Heterologous Protein Expression

The impact of polyadenylation signals on plasmid nuclease-resistance and transgene expression

The role of polyadenylation signal secondary structures on the resistance of plasmid vectors to nucleases.

The role of probe–probe interactions on the hybridization of double-stranded DNA targets onto DNA-modified magnetic microparticles

Time-course determination of plasmid content in eukaryotic and prokaryotic cells using real-time PCR.

Towards effective non-viral gene delivery vector.

Towards the miniaturization of GPCR-based live-cell screening assays.

Trans-sialidase from Trypanosoma brucei as a potential target for DNA vaccine development against African trypanosomiasis

Translational features of human alpha 2b interferon production in Escherichia coli.

Troubleshooting in Gene Splicing by Overlap Extension: A Step-Wise Method

Trypanosoma brucei: immunisation with plasmid DNA encoding invariant surface glycoprotein gene is able to induce partial protection in experimental African trypanosomiasis.