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List of works by José G Gavilanes

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Crystal and electron microscopy structures of sticholysin II actinoporin reveal insights into the mechanism of membrane pore formation

scientific article
Cytotoxic mechanism of the ribotoxin alpha-sarcin. Induction of cell death via apoptosis

scientific article
NMR solution structure of the antifungal protein from Aspergillus giganteus: evidence for cysteine pairing isomerism

scientific article
Characterization of the antifungal protein secreted by the mould Aspergillus giganteus

scientific article
The behavior of sea anemone actinoporins at the water-membrane interface

scientific article published on 20 May 2011
Characterization of pKa values and titration shifts in the cytotoxic ribonuclease alpha-sarcin by NMR. Relationship between electrostatic interactions, structure, and catalytic function.

scientific article published in November 1998
Mechanism of the leakage induced on lipid model membranes by the hemolytic protein sticholysin II from the sea anemone Stichodactyla helianthus

scientific article published on 01 March 1998
Overproduction and purification of biologically active native fungal alpha-sarcin in Escherichia coli.

scientific article
Anomalous electrophoretic behavior of a very acidic protein: ribonuclease U2.

scientific article published in September 2005
Kinetic study of the cytotoxic effect of alpha-sarcin, a ribosome inactivating protein from Aspergillus giganteus, on tumour cell lines: protein biosynthesis inhibition and cell binding

scientific article published on 01 May 1993
A permanent Zn2+ reverse staining method for the detection and quantification of proteins in polyacrylamide gels.

scientific article published in September 1993
Deletion of the NH2-terminal beta-hairpin of the ribotoxin alpha-sarcin produces a nontoxic but active ribonuclease

scientific article
Cleavage of the sarcin-ricin loop of 23S rRNA differentially affects EF-G and EF-Tu binding

scientific article
Sticholysin II, a cytolysin from the sea anemone Stichodactyla helianthus , is a monomer-tetramer associating protein

article
Release of Lipid Vesicle Contents by an Antibacterial Cecropin A−Melittin Hybrid Peptide†

scientific article published on 01 July 1996
Role of histidine-50, glutamic acid-96, and histidine-137 in the ribonucleolytic mechanism of the ribotoxin alpha-sarcin

scientific article published on 01 November 1999
The cytotoxin α-sarcin behaves as a cyclizing ribonuclease

scientific article published on 01 March 1998
Three-dimensional structure of the actinoporin sticholysin I. Influence of long-distance effects on protein function.

scientific article
Involvement of the amino-terminal beta-hairpin of the Aspergillus ribotoxins on the interaction with membranes and nonspecific ribonuclease activity.

scientific article published on August 2001
Predictive study of the conformation of the cytotoxic protein α-sarcin: a structural model to explain α-sarcin-membrane interaction

scientific article published on 01 February 1995
Calorimetric scrutiny of lipid binding by sticholysin II toxin mutants

scientific article published on 26 July 2008
Arginine 121 is a crucial residue for the specific cytotoxic activity of the ribotoxin alpha-sarcin

scientific article published in December 2001
The role of electrostatic interactions in the antitumor activity of dimeric RNases

scientific article published on August 2006
Production and characterization of a noncytotoxic deletion variant of the Aspergillus fumigatus allergen Aspf1 displaying reduced IgE binding.

scientific article
Production and characterization of scFvA33T1, an immunoRNase targeting colon cancer cells

scientific article published on 25 July 2012
Acid phospholipid vesicles produce conformational changes on the antitumour protein alpha-sarcin

scientific article published in October 1991
Effect of the antitumour protein α-sarcin on the thermotropic behaviour of acid phospholipid vesicles

scientific article published on 01 September 1991
Establishment and characterization of a new human colon adenocarcinoma cell line: BCS-TC2.

scientific article published on January 1990
Structural basis for the catalytic mechanism and substrate specificity of the ribonuclease α-sarcin

scientific article published on 01 December 1996
The insecticidal protein hirsutellin A from the mite fungal pathogen Hirsutella thompsonii is a ribotoxin

scientific article published in July 2008
Fluorometric detection of peptides after column chromatography or on paper: o-phthalaldehyde and fluorescamine

scientific article published on 01 May 1976
Detergent-resistant membranes are platforms for actinoporin pore-forming activity on intact cells.

scientific article published in February 2006
Sequence determination and molecular characterization of gigantin, a cytotoxic protein produced by the mould Aspergillus giganteus IFO 5818.

scientific article published in July 1997
Secretion of recombinant pro- and mature fungal alpha-sarcin ribotoxin by the methylotrophic yeast Pichia pastoris: the Lys-Arg motif is required for maturation.

scientific article
2NH and 3OH are crucial structural requirements in sphingomyelin for sticholysin II binding and pore formation in bilayer membranes.

scientific article published on 30 January 2013
Oligomerization of the cytotoxin α-sarcin associated with phospholipid membranes

scientific article published on 01 July 1998
Dissecting structural and electrostatic interactions of charged groups in alpha-sarcin. An NMR study of some mutants involving the catalytic residues.

scientific article published in November 2003
Efficient in vivo antitumor effect of an immunotoxin based on ribotoxin α-sarcin in nude mice bearing human colorectal cancer xenografts.

scientific article published on 8 April 2015
Fungal ribotoxins: Natural protein-based weapons against insects

scientific article published on 14 March 2014
Cholesterol stimulates and ceramide inhibits Sticholysin II-induced pore formation in complex bilayer membranes

scientific article
A complementary microscopy analysis of Sticholysin II crystals on lipid films: Atomic force and transmission electron characterizations

scientific article published on 12 October 2005
Intrinsic local disorder and a network of charge-charge interactions are key to actinoporin membrane disruption and cytotoxicity

scientific article
Leucine 145 of the ribotoxin alpha-sarcin plays a key role for determining the specificity of the ribosome-inactivating activity of the protein.

scientific article published on January 2003
Assignment of the contribution of the tryptophan residues to the spectroscopic and functional properties of the ribotoxin alpha-sarcin

scientific article published on 01 November 2000
Lysozyme from the Insect Ceratitis capitata Eggs

scientific article published on January 3, 1977
The sea anemone actinoporin (Arg-Gly-Asp) conserved motif is involved in maintaining the competent oligomerization state of these pore-forming toxins

scientific article published on 06 February 2014
Backbone dynamics of the cytotoxic ribonuclease alpha-sarcin by 15N NMR relaxation methods.

scientific article
Fluorescence studies on the lipoprotein complex of the fatty acid synthetase from the insect Ceratitis capitata

scientific article published in September 1981
Overproduction in Escherichia coli and Purification of the Hemolytic Protein Sticholysin II from the Sea Anemone Stichodactyla helianthus

scientific article published on 01 February 2000
Hirsutellin A Displays Significant Homology to Microbial Extracellular Ribonucleases

scientific article published on 01 July 1999

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