List of works - Javier Lacadena

1H and 15N nuclear magnetic resonance assignment and secondary structure of the cytotoxic ribonuclease alpha-Sarcin

scientific article published on May 1996

A non-cytotoxic but ribonucleolytically specific ribotoxin variant: implication of tryptophan residues in the cytotoxicity of hirsutellin A.

scientific article published on May 2012

Anomalous electrophoretic behavior of a very acidic protein: ribonuclease U2.

scientific article published in September 2005

Arginine 121 is a crucial residue for the specific cytotoxic activity of the ribotoxin alpha-sarcin

scientific article published in December 2001

Assignment of the contribution of the tryptophan residues to the spectroscopic and functional properties of the ribotoxin alpha-sarcin

scientific article published on 01 November 2000

Characterization of a natural larger form of the antifungal protein (AFP) from Aspergillus giganteus

scientific article published on 01 June 1997

Characterization of pKa values and titration shifts in the cytotoxic ribonuclease alpha-sarcin by NMR. Relationship between electrostatic interactions, structure, and catalytic function.

scientific article published in November 1998

Characterization of the antifungal protein secreted by the mould Aspergillus giganteus.

scientific article

Dissecting structural and electrostatic interactions of charged groups in alpha-sarcin. An NMR study of some mutants involving the catalytic residues.

scientific article published in November 2003

Efficient in vivo antitumor effect of an immunotoxin based on ribotoxin α-sarcin in nude mice bearing human colorectal cancer xenografts.

scientific article published on 8 April 2015

Efficient production of single-chain fragment variable-based N-terminal trimerbodies in Pichia pastoris

scientific article published on 12 August 2014

Escherichia coli JA221 can suppress the UAG stop signal.

scientific article

Expression of homeotic genes Hoxa3, Hoxb3, Hoxd3 and Hoxc4 is decreased in the lungs but not in the hearts of adriamycin-exposed mice.

scientific article published in May 2007

Hirsutellin A Displays Significant Homology to Microbial Extracellular Ribonucleases

scientific article published on 01 July 1999

Hirsutellin A: A Paradigmatic Example of the Insecticidal Function of Fungal Ribotoxins.

scientific article published on 09 July 2013

Implication of an Asp residue in the ribonucleolytic activity of hirsutellin A reveals new electrostatic interactions at the active site of ribotoxins

scientific article published on 24 August 2011

Involvement of loops 2 and 3 of α-sarcin on its ribotoxic activity.

scientific article published on 15 January 2015

Involvement of the amino-terminal beta-hairpin of the Aspergillus ribotoxins on the interaction with membranes and nonspecific ribonuclease activity.

scientific article published on August 2001

Kinetic study of the aggregation and lipid mixing produced by alpha-sarcin on phosphatidylglycerol and phosphatidylserine vesicles: stopped-flow light scattering and fluorescence energy transfer measurements

scientific article published on September 1994

Membrane interaction of a beta-structure-forming synthetic peptide comprising the 116-139th sequence region of the cytotoxic protein alpha-sarcin

scientific article published on June 1995

NMR solution structure of the antifungal protein from Aspergillus giganteus: evidence for cysteine pairing isomerism

scientific article

Oligomerization of the cytotoxin α-sarcin associated with phospholipid membranes

scientific article published on 01 July 1998

Overproduction and purification of biologically active native fungal alpha-sarcin in Escherichia coli.

scientific article

Overproduction in Escherichia coli and Purification of the Hemolytic Protein Sticholysin II from the Sea Anemone Stichodactyla helianthus

scientific article published on 01 February 2000

Predictive study of the conformation of the cytotoxic protein α-sarcin: a structural model to explain α-sarcin-membrane interaction

scientific article published on 01 February 1995

Preparation of an engineered safer immunotoxin against colon carcinoma based on the ribotoxin hirsutellin A.

scientific article published on 27 March 2015

Production and characterization of a noncytotoxic deletion variant of the Aspergillus fumigatus allergen Aspf1 displaying reduced IgE binding.

scientific article

Production and characterization of scFvA33T1, an immunoRNase targeting colon cancer cells

scientific article published on 25 July 2012

RNase U2 and alpha-sarcin: a study of relationships

scientific article published on 01 January 2001

Role of histidine-50, glutamic acid-96, and histidine-137 in the ribonucleolytic mechanism of the ribotoxin alpha-sarcin

scientific article published on 01 November 1999

Secretion of recombinant pro- and mature fungal alpha-sarcin ribotoxin by the methylotrophic yeast Pichia pastoris: the Lys-Arg motif is required for maturation.

scientific article

Sequence determination and molecular characterization of gigantin, a cytotoxic protein produced by the mould Aspergillus giganteus IFO 5818.

scientific article published in July 1997

Spectroscopic characterization of the alkylated alpha-sarcin cytotoxin: analysis of the structural requirements for the protein-lipid bilayer hydrophobic interaction

scientific article published in September 1995

Structural basis for the catalytic mechanism and substrate specificity of the ribonuclease α-sarcin

scientific article published on 01 December 1996

Substitution of histidine-137 by glutamine abolishes the catalytic activity of the ribosome-inactivating protein alpha-sarcin

scientific article published on July 1995

The highly refined solution structure of the cytotoxic ribonuclease alpha-sarcin reveals the structural requirements for substrate recognition and ribonucleolytic activity

scientific article

The insecticidal protein hirsutellin A from the mite fungal pathogen Hirsutella thompsonii is a ribotoxin.

scientific article published in July 2008

Thermal unfolding of the cytotoxin alpha-sarcin: phospholipid binding induces destabilization of the protein structure.

scientific article

Translocation of alpha-sarcin across the lipid bilayer of asolectin vesicles.

scientific article published on October 1993

Trispecific T-cell engagers for dual tumor-targeting of colorectal cancer

scientific article published on 07 February 2022

α-sarcin and RNase T1 based immunoconjugates: the role of intracellular trafficking in cytotoxic efficiency.

scientific article

List of works by Javier Lacadena

1H and 15N nuclear magnetic resonance assignment and secondary structure of the cytotoxic ribonuclease alpha-Sarcin

A non-cytotoxic but ribonucleolytically specific ribotoxin variant: implication of tryptophan residues in the cytotoxicity of hirsutellin A.

Anomalous electrophoretic behavior of a very acidic protein: ribonuclease U2.

Arginine 121 is a crucial residue for the specific cytotoxic activity of the ribotoxin alpha-sarcin

Assignment of the contribution of the tryptophan residues to the spectroscopic and functional properties of the ribotoxin alpha-sarcin

Characterization of a natural larger form of the antifungal protein (AFP) from Aspergillus giganteus

Characterization of pKa values and titration shifts in the cytotoxic ribonuclease alpha-sarcin by NMR. Relationship between electrostatic interactions, structure, and catalytic function.

Characterization of the antifungal protein secreted by the mould Aspergillus giganteus.

Dissecting structural and electrostatic interactions of charged groups in alpha-sarcin. An NMR study of some mutants involving the catalytic residues.

Efficient in vivo antitumor effect of an immunotoxin based on ribotoxin α-sarcin in nude mice bearing human colorectal cancer xenografts.

Efficient production of single-chain fragment variable-based N-terminal trimerbodies in Pichia pastoris

Escherichia coli JA221 can suppress the UAG stop signal.

Expression of homeotic genes Hoxa3, Hoxb3, Hoxd3 and Hoxc4 is decreased in the lungs but not in the hearts of adriamycin-exposed mice.

Hirsutellin A Displays Significant Homology to Microbial Extracellular Ribonucleases

Hirsutellin A: A Paradigmatic Example of the Insecticidal Function of Fungal Ribotoxins.

Implication of an Asp residue in the ribonucleolytic activity of hirsutellin A reveals new electrostatic interactions at the active site of ribotoxins

Involvement of loops 2 and 3 of α-sarcin on its ribotoxic activity.

Involvement of the amino-terminal beta-hairpin of the Aspergillus ribotoxins on the interaction with membranes and nonspecific ribonuclease activity.

Kinetic study of the aggregation and lipid mixing produced by alpha-sarcin on phosphatidylglycerol and phosphatidylserine vesicles: stopped-flow light scattering and fluorescence energy transfer measurements

Membrane interaction of a beta-structure-forming synthetic peptide comprising the 116-139th sequence region of the cytotoxic protein alpha-sarcin

NMR solution structure of the antifungal protein from Aspergillus giganteus: evidence for cysteine pairing isomerism

Oligomerization of the cytotoxin α-sarcin associated with phospholipid membranes

Overproduction and purification of biologically active native fungal alpha-sarcin in Escherichia coli.

Overproduction in Escherichia coli and Purification of the Hemolytic Protein Sticholysin II from the Sea Anemone Stichodactyla helianthus

Predictive study of the conformation of the cytotoxic protein α-sarcin: a structural model to explain α-sarcin-membrane interaction

Preparation of an engineered safer immunotoxin against colon carcinoma based on the ribotoxin hirsutellin A.

Production and characterization of a noncytotoxic deletion variant of the Aspergillus fumigatus allergen Aspf1 displaying reduced IgE binding.

Production and characterization of scFvA33T1, an immunoRNase targeting colon cancer cells

RNase U2 and alpha-sarcin: a study of relationships

Role of histidine-50, glutamic acid-96, and histidine-137 in the ribonucleolytic mechanism of the ribotoxin alpha-sarcin

Secretion of recombinant pro- and mature fungal alpha-sarcin ribotoxin by the methylotrophic yeast Pichia pastoris: the Lys-Arg motif is required for maturation.

Sequence determination and molecular characterization of gigantin, a cytotoxic protein produced by the mould Aspergillus giganteus IFO 5818.

Spectroscopic characterization of the alkylated alpha-sarcin cytotoxin: analysis of the structural requirements for the protein-lipid bilayer hydrophobic interaction

Structural basis for the catalytic mechanism and substrate specificity of the ribonuclease α-sarcin

Substitution of histidine-137 by glutamine abolishes the catalytic activity of the ribosome-inactivating protein alpha-sarcin

The highly refined solution structure of the cytotoxic ribonuclease alpha-sarcin reveals the structural requirements for substrate recognition and ribonucleolytic activity

The insecticidal protein hirsutellin A from the mite fungal pathogen Hirsutella thompsonii is a ribotoxin.

Thermal unfolding of the cytotoxin alpha-sarcin: phospholipid binding induces destabilization of the protein structure.

Translocation of alpha-sarcin across the lipid bilayer of asolectin vesicles.

Trispecific T-cell engagers for dual tumor-targeting of colorectal cancer

α-sarcin and RNase T1 based immunoconjugates: the role of intracellular trafficking in cytotoxic efficiency.