List of works - José A. Gavira

A multiple path photonic lab on a chip for parallel protein concentration measurements

scientific article published in February 2015

A novel cysteine carbamoyl-switch is responsible for the inhibition of formamidase, a nitrilase superfamily member

scientific article published on 05 December 2018

A simple and versatile microfluidic device for efficient biomacromolecule crystallization and structural analysis by serial crystallography

scientific article published on 19 April 2019

Ab initiocrystallographic structure determination of insulin from protein to electron density without crystal handling

scientific article published on 20 June 2002

Agarose as crystallisation media for proteins II: Trapping of gel fibres into the crystals

scientific article published on 26 September 2002

Attaining atomic resolution from in situ data collection at room temperature using counter-diffusion-based low-cost microchips

scientific article published on 27 July 2020

Binding curves by continuous gradient flow-mix calorimetry

Biophysical and atomic force microscopy characterization of the RNA from satellite tobacco mosaic virus ⬇️

scientific article published on August 6, 2010

Capillary crystallization and molecular-replacement solution of haemoglobin II from the clam Lucina pectinata

scientific article published on 10 February 2006

Chemotaxis of the Human Pathogen Pseudomonas aeruginosa to the Neurotransmitter Acetylcholine

scientific article published on 07 March 2022

Cloning, expression, purification, crystallization and preliminary X-ray characterization of allantoinase from Bacillus licheniformis ATCC 14580.

scientific article published on 25 October 2014

Continuous Sensing Photonic Lab-on-a-Chip Platform Based on Cross-Linked Enzyme Crystals.

scientific article published on 26 October 2016

Crystallization and crystallographic analysis of the ligand-binding domain of the Pseudomonas putida chemoreceptor McpS in complex with malate and succinate

scientific article

Crystallization and diffraction patterns of the oxy and cyano forms of the Lucina pectinata haemoglobins complex.

scientific article published on 25 December 2008

Crystallization and preliminary crystallographic studies of an active-site mutant hydantoin racemase from Sinorhizobium meliloti CECT4114.

scientific article

Crystallization and preliminary crystallographic studies of the recombinant L-N-carbamoylase from Geobacillus stearothermophilus CECT43

scientific article published on 28 November 2008

Crystallization and preliminary crystallographic studies of the recombinant dihydropyrimidinase from Sinorhizobium meliloti CECT4114.

scientific article published on 30 November 2006

Crystallization by capillary counter-diffusion and structure determination of the N114A mutant of the SH3 domain of Abl tyrosine kinase complexed with a high-affinity peptide ligand

scientific article

Current trends in protein crystallization.

scientific article

De novo active sites for resurrected Precambrian enzymes

scientific article

Efficacy of aldose reductase inhibitors is affected by oxidative stress induced under X-ray irradiation ⬇️

scientific article published on 28 February 2019

Enhanced vulnerability of human proteins towards disease-associated inactivation through divergent evolution

scientific article

Enhancing a de novo enzyme activity by computationally-focused ultra-low-throughput screening

scientific article published on 19 May 2020

Evidence for Pentapeptide-Dependent and Independent CheB Methylesterases

scientific article published on 11 November 2020

Evidence for chemoreceptors with bimodular ligand-binding regions harboring two signal-binding sites

scientific article

Evolution of conformational dynamics determines the conversion of a promiscuous generalist into a specialist enzyme.

scientific article

Force-clamp spectroscopy detects residue co-evolution in enzyme catalysis

scientific article published on 7 August 2008

Functional Annotation of Bacterial Signal Transduction Systems: Progress and Challenges

scientific article published on 26 November 2018

Granada Crystallisation Box: a new device for protein crystallisation by counter-diffusion techniques

scientific article published on 26 September 2002

Heme-binding enables allosteric modulation in an ancient TIM-barrel glycosidase

scientific article published on 15 January 2021

High-Affinity Chemotaxis to Histamine Mediated by the TlpQ Chemoreceptor of the Human Pathogen Pseudomonas aeruginosa

scientific article published on 13 November 2018

How Bacterial Chemoreceptors Evolve Novel Ligand Specificities

scientific article published on 21 January 2020

Hyperstability and substrate promiscuity in laboratory resurrections of Precambrian β-lactamases

scientific article

Identification and characterization of a bacterial hyaluronidase and its production in recombinant form

scientific article

In situ X-ray data collection from highly sensitive crystals of Pseudomonas putida PtxS in complex with DNA.

scientific article

In-situmeasurement of rocking curves during lysozyme crystal growth

scientific article published on 01 March 1999

Influence of the chirality of short peptide supramolecular hydrogels in protein crystallogenesis

scientific article

Introduction to protein crystallization

scientific article published on 24 December 2013

Macromolecular crystallography: An old science with new perspectives

scientific article

McCLEC, a robust and stable enzymatic based microreactor platform

scientific article published on 3 September 2015

Modulation of buried ionizable groups in proteins with engineered surface charge.

scientific article

Monitoring and scoring counter-diffusion protein crystallization experiments in capillaries by in situ dynamic light scattering

scientific article

Mutational and Structural Analysis of L-N-Carbamoylase Reveals New Insights into a Peptidase M20/M25/M40 Family Member

scientific article

Mutational studies on resurrected ancestral proteins reveal conservation of site-specific amino acid preferences throughout evolutionary history

scientific article

New techniques for membrane protein crystallization tested on photosystem II core complex of Pisum sativum

scientific article published on 01 December 2006

Novel conformational aspects of the third PDZ domain of the neuronal post-synaptic density-95 protein revealed from two 1.4A X-ray structures

scientific article

Phenotypic comparisons of consensus variants versus laboratory resurrections of Precambrian proteins

scientific article

Production of Cross-Linked Lipase Crystals at a Preparative Scale

scientific article published on 17 February 2021

Protein crystallization by capillary counterdiffusion for applied crystallographic structure determination.

scientific article

Protein separation under a microfluidic regime.

scientific article published on 7 December 2017

Purification, crystallization and preliminary X-ray analysis of mexicain.

scientific article

Purification, crystallization and preliminary crystallographic analysis of the ligand-binding regions of the PctA and PctB chemoreceptors from Pseudomonas aeruginosa in complex with amino acids.

scientific article published on 29 November 2013

Seeding from silica-reinforced lysozyme crystals for neutron crystallography

scientific article published on 30 November 2018

Structural Basis for Polyamine Binding at the dCACHE Domain of the McpU Chemoreceptor from Pseudomonas putida

scientific article published on 11 May 2018

Structural insights into choline-O-sulfatase reveal the molecular determinants for ligand binding

scientific article published on 26 April 2022

Structure and Ligand Selection of Hemoglobin II from Lucina pectinata

scientific article

Structure of dihydropyrimidinase from Sinorhizobium meliloti CECT4114: new features in an amidohydrolase family member

scientific article

Structure of the mexicain-E-64 complex and comparison with other cysteine proteases of the papain family.

scientific article

The Molecular Mechanism of Nitrate Chemotaxis via Direct Ligand Binding to the PilJ Domain of McpN

scientific article published on 19 February 2019

Thermostable and promiscuous Precambrian proteins

scientific article published on 01 June 2014

Two-step counterdiffusion protocol for the crystallization of haemoglobin II from Lucina pectinata in the pH range 4-9.

scientific article

Understanding the polymorphic behaviour of a mutant of the α-spectrin SH3 domain by means of two 1.1 Å resolution structures

scientific article

Use of Cross-Linked Poly(ethylene glycol)-Based Hydrogels for Protein Crystallization

scientific article

List of works by José A. Gavira

A multiple path photonic lab on a chip for parallel protein concentration measurements

A novel cysteine carbamoyl-switch is responsible for the inhibition of formamidase, a nitrilase superfamily member

A simple and versatile microfluidic device for efficient biomacromolecule crystallization and structural analysis by serial crystallography

Ab initiocrystallographic structure determination of insulin from protein to electron density without crystal handling

Agarose as crystallisation media for proteins II: Trapping of gel fibres into the crystals

Attaining atomic resolution from in situ data collection at room temperature using counter-diffusion-based low-cost microchips

Binding curves by continuous gradient flow-mix calorimetry

Biophysical and atomic force microscopy characterization of the RNA from satellite tobacco mosaic virus ⬇️

Capillary crystallization and molecular-replacement solution of haemoglobin II from the clam Lucina pectinata

Chemotaxis of the Human Pathogen Pseudomonas aeruginosa to the Neurotransmitter Acetylcholine

Cloning, expression, purification, crystallization and preliminary X-ray characterization of allantoinase from Bacillus licheniformis ATCC 14580.

Continuous Sensing Photonic Lab-on-a-Chip Platform Based on Cross-Linked Enzyme Crystals.

Crystallization and crystallographic analysis of the ligand-binding domain of the Pseudomonas putida chemoreceptor McpS in complex with malate and succinate

Crystallization and diffraction patterns of the oxy and cyano forms of the Lucina pectinata haemoglobins complex.

Crystallization and preliminary crystallographic studies of an active-site mutant hydantoin racemase from Sinorhizobium meliloti CECT4114.

Crystallization and preliminary crystallographic studies of the recombinant L-N-carbamoylase from Geobacillus stearothermophilus CECT43

Crystallization and preliminary crystallographic studies of the recombinant dihydropyrimidinase from Sinorhizobium meliloti CECT4114.

Crystallization by capillary counter-diffusion and structure determination of the N114A mutant of the SH3 domain of Abl tyrosine kinase complexed with a high-affinity peptide ligand

Current trends in protein crystallization.

De novo active sites for resurrected Precambrian enzymes

Efficacy of aldose reductase inhibitors is affected by oxidative stress induced under X-ray irradiation ⬇️

Enhanced vulnerability of human proteins towards disease-associated inactivation through divergent evolution

Enhancing a de novo enzyme activity by computationally-focused ultra-low-throughput screening

Evidence for Pentapeptide-Dependent and Independent CheB Methylesterases

Evidence for chemoreceptors with bimodular ligand-binding regions harboring two signal-binding sites

Evolution of conformational dynamics determines the conversion of a promiscuous generalist into a specialist enzyme.

Force-clamp spectroscopy detects residue co-evolution in enzyme catalysis

Functional Annotation of Bacterial Signal Transduction Systems: Progress and Challenges

Granada Crystallisation Box: a new device for protein crystallisation by counter-diffusion techniques

Heme-binding enables allosteric modulation in an ancient TIM-barrel glycosidase

High-Affinity Chemotaxis to Histamine Mediated by the TlpQ Chemoreceptor of the Human Pathogen Pseudomonas aeruginosa

How Bacterial Chemoreceptors Evolve Novel Ligand Specificities

Hyperstability and substrate promiscuity in laboratory resurrections of Precambrian β-lactamases

Identification and characterization of a bacterial hyaluronidase and its production in recombinant form

In situ X-ray data collection from highly sensitive crystals of Pseudomonas putida PtxS in complex with DNA.

In-situmeasurement of rocking curves during lysozyme crystal growth

Influence of the chirality of short peptide supramolecular hydrogels in protein crystallogenesis

Introduction to protein crystallization

Macromolecular crystallography: An old science with new perspectives

McCLEC, a robust and stable enzymatic based microreactor platform

Modulation of buried ionizable groups in proteins with engineered surface charge.

Monitoring and scoring counter-diffusion protein crystallization experiments in capillaries by in situ dynamic light scattering

Mutational and Structural Analysis of L-N-Carbamoylase Reveals New Insights into a Peptidase M20/M25/M40 Family Member

Mutational studies on resurrected ancestral proteins reveal conservation of site-specific amino acid preferences throughout evolutionary history

New techniques for membrane protein crystallization tested on photosystem II core complex of Pisum sativum

Novel conformational aspects of the third PDZ domain of the neuronal post-synaptic density-95 protein revealed from two 1.4A X-ray structures

Phenotypic comparisons of consensus variants versus laboratory resurrections of Precambrian proteins

Production of Cross-Linked Lipase Crystals at a Preparative Scale

Protein crystallization by capillary counterdiffusion for applied crystallographic structure determination.

Protein separation under a microfluidic regime.

Purification, crystallization and preliminary X-ray analysis of mexicain.

Purification, crystallization and preliminary crystallographic analysis of the ligand-binding regions of the PctA and PctB chemoreceptors from Pseudomonas aeruginosa in complex with amino acids.

Seeding from silica-reinforced lysozyme crystals for neutron crystallography

Structural Basis for Polyamine Binding at the dCACHE Domain of the McpU Chemoreceptor from Pseudomonas putida

Structural insights into choline-O-sulfatase reveal the molecular determinants for ligand binding

Structure and Ligand Selection of Hemoglobin II from Lucina pectinata

Structure of dihydropyrimidinase from Sinorhizobium meliloti CECT4114: new features in an amidohydrolase family member

Structure of the mexicain-E-64 complex and comparison with other cysteine proteases of the papain family.

The Molecular Mechanism of Nitrate Chemotaxis via Direct Ligand Binding to the PilJ Domain of McpN

Thermostable and promiscuous Precambrian proteins

Two-step counterdiffusion protocol for the crystallization of haemoglobin II from Lucina pectinata in the pH range 4-9.

Understanding the polymorphic behaviour of a mutant of the α-spectrin SH3 domain by means of two 1.1 Å resolution structures

Use of Cross-Linked Poly(ethylene glycol)-Based Hydrogels for Protein Crystallization