Search filters

List of works by Manuel J T Carrondo

293 cell cycle synchronisation adenovirus vector production

scientific article

A cell sorting protocol for selecting high-producing sub-populations of Sf9 and High Five™ cells

scientific article published on 24 October 2013

A detection and quantification label-free tool to speed up downstream processing of model mucins

scientific article published on 9 January 2018

A new generation of retroviral producer cells: predictable and stable virus production by Flp-mediated site-specific integration of retroviral vectors.

scientific article published on 11 May 2006

A novel recombinant virus-like particle vaccine for prevention of porcine parvovirus-induced reproductive failure.

scientific article published on 18 April 2006

A procedure for high-yield spore production by Bacillus subtilis.

scientific article published in July 2005

Adaptation of BHK cells producing a recombinant protein to serum-free media and protein-free medium

scientific article published on January 1998

Adaptive dissolved oxygen control through the glycerol feeding in a recombinant Pichia pastoris cultivation in conditions of oxygen transfer limitation.

scientific article published on 10 December 2004

Adenovirus purification by two-column, size-exclusion, simulated countercurrent chromatography

scientific article published on 2 May 2014

Advances in heterologous protein production

scientific article published on 14 July 2005

Advances in on-line monitoring and control of mammalian cell cultures: Supporting the PAT initiative.

scientific article published on 18 May 2009

An integrated strategy for the process development of a recombinant antibody-cytokine fusion protein expressed in BHK cells.

scientific article

Analysis of adsorption of a baculovirus bioreaction bulk on an ion-exchange surface by surface plasmon resonance.

scientific article published on 26 May 2010

Anion-exchange membrane chromatography for purification of rotavirus-like particles

Baculovirus production for gene therapy: the role of cell density, multiplicity of infection and medium exchange

scientific article published on 16 October 2008

Biochemical engineering

scientific article published in October 2004

Bioorthogonal Strategy for Bioprocessing of Specific-Site-Functionalized Enveloped Influenza-Virus-Like Particles

scientific article published on 21 September 2016

Bioprocess integration for human mesenchymal stem cells: From up to downstream processing scale-up to cell proteome characterization

scientific article published on 4 February 2017

Bioprocess iterative batch-to-batch optimization based on hybrid parametric/nonparametric models

scientific article published in January 2006

Catalase effect on cell death for the improvement of recombinant protein production in baculovirus-insect cell system.

scientific article published on 3 November 2006

Cell density effect in the baculovirus-insect cells system: a quantitative analysis of energetic metabolism

scientific article

Cell functional enviromics: unravelling the function of environmental factors.

scientific article

Cell growth arrest by nucleotides, nucleosides and bases as a tool for improved production of recombinant proteins

scientific article published on January 2003

Cell-dislodging methods under serum-free conditions

scientific article published on May 1997

Cellular targets for improved manufacturing of virus-based biopharmaceuticals in animal cells

scientific article

Challenges in vector and trial design using retroviral vectors for long-term gene correction in hematopoietic stem cell gene therapy

scientific article published in June 2012

Changes in animal cell natural aggregates in suspended batch cultures

article

Characterization and downstream processing of HIV-1 core and virus-like-particles produced in serum free medium

article

Characterization of Ehrlichia ruminantium replication and release kinetics in endothelial cell cultures.

scientific article

Characterization of a highly thermostable extracellular lipase from Lactobacillus plantarum

scientific article published in June 2002

Clinical-Grade Oncolytic Adenovirus Purification Using Polysorbate 20 as an Alternative for Cell Lysis

scientific article

Combining hypoxia and bioreactor hydrodynamics boosts induced pluripotent stem cell differentiation towards cardiomyocytes

scientific article

Combining stable insect cell lines with baculovirus-mediated expression for multi-HA influenza VLP production.

scientific article published on 10 March 2017

Comparison of different bioreactor systems for the production of high titer retroviral vectors

scientific article published on March 2001

Cryopreservation in micro-volumes: impact upon Caco-2 colon adenocarcinoma cell proliferation and differentiation

scientific article

Cultures of rat astrocytes challenged with a steady supply of glutamate: new model to study flux distribution in the glutamate-glutamine cycle

scientific article published in September 2005

Culturing primary brain astrocytes under a fully controlled environment in a novel bioreactor

scientific article published in January 2005

Disclosing the Parameters Leading to High Productivity of Retroviral Producer Cells Lines: Evaluating Random Versus Targeted Integration.

scientific article published on 16 March 2017

Down-regulation of CD81 tetraspanin in human cells producing retroviral-based particles: tailoring vector composition.

scientific article published on 21 June 2011

Downstream Processing of Lentiviral Vectors: Releasing Bottlenecks ⬇️

scientific article published on August 30, 2012

Downstream processing of triple layered rotavirus like particles

scientific article published on 5 August 2006

Editorial [Hot topic: GMP Production of Gene and Cell Therapy Therapeutics (Guest Editors: Manuel Carrondo and Pedro Cruz)] ⬇️

scientific article published on December 1, 2010

Effect of ammonia production on intracellular pH: Consequent effect on adenovirus vector production

scientific article published on 23 January 2007

Effect of ethanol on the metabolism of primary astrocytes studied by 13C‐ and 31P‐NMR spectroscopy ⬇️

scientific article published on December 1, 2001

Effect of medium sugar source on the production of retroviral vectors for gene therapy.

scientific article published on May 2006

Effect of osmotic pressure on the production of retroviral vectors: Enhancement in vector stability.

scientific article published on June 2006

Effect of re-feed strategies and non-ammoniagenic medium on adenovirus production at high cell densities

scientific article

Effect of the purification process and the storage conditions on the efficacy of an inactivated vaccine against heartwater

scientific article published on 7 May 2007

Effect of viscosity upon hydrodynamically controlled natural aggregates of animal cells grown in stirred vessels

scientific article published on 01 September 1995

Effects of ammonia and lactate on growth, metabolism, and productivity of BHK cells

scientific article published on 01 July 2000

Enhancement of Sf-9 cell growth and longevity through supplementation of culture medium with hemolymph

scientific article published on January 2003

Error assessment in recombinant baculovirus titration: evaluation of different methods.

scientific article

Evaluation of novel large cut-off ultrafiltration membranes for adenovirus serotype 5 (Ad5) concentration.

scientific article published on 29 December 2014

Exploring analytical proteomics platforms toward the definition of human cardiac stem cells receptome

scientific article published on 05 February 2015

Exploring continuous and integrated strategies for the up- and downstream processing of human mesenchymal stem cells

scientific article published on 4 March 2015

Extending hepatocyte functionality for drug-testing applications using high-viscosity alginate-encapsulated three-dimensional cultures in bioreactors

scientific article published on 6 April 2010

Extracellular purine and pyrimidine catabolism in cell culture

scientific article published on 21 June 2011

Filtration methodologies for the clarification and concentration of human mesenchymal stem cells

Finding the design space of a filtration-based operation for the concentration of human pluripotent stem cells

Formation and disruption of animal cell aggregates in stirred vessels: Mechanisms and kinetic studies

From retroviral vector production to gene transfer: spontaneous inactivation is caused by loss of reverse transcription capacity.

scientific article published on April 2008

High-throughput analysis of animal cell cultures using two-dimensional fluorometry

scientific article published on 27 November 2010

How can measurement, monitoring, modeling and control advance cell culture in industrial biotechnology?

article

Human amniocyte-derived cells are a promising cell host for adenoviral vector production under serum-free conditions

scientific article published on May 2015

Human liver cell spheroids in extended perfusion bioreactor culture for repeated-dose drug testing

scientific article

Hybrid elementary flux analysis/nonparametric modeling: application for bioprocess control

scientific article published on 29 January 2007

Hybrid metabolic flux analysis: combining stoichiometric and statistical constraints to model the formation of complex recombinant products

scientific article

Hybrid semi-parametric mathematical systems: bridging the gap between systems biology and process engineering.

scientific article published on 15 August 2007

Immobilization of Primary Astrocytes and Neurons for On-Line Monitoring of Biochemical Processes by NMR

Impact of grafting on the design of new membrane adsorbers for adenovirus purification.

scientific article published on 18 April 2014

Impact of ligand density on the optimization of ion-exchange membrane chromatography for viral vector purification.

scientific article published on 3 February 2011

Impact of physicochemical parameters on in vitro assembly and disassembly kinetics of recombinant triple-layered rotavirus-like particles.

scientific article published in November 2009

Impact of retroviral vector components stoichiometry on packaging cell lines: effects on productivity and vector quality

scientific article published on February 2008

Improved virus purification processes for vaccines and gene therapy

scientific article

Improving baculovirus production at high cell density through manipulation of energy metabolism

scientific article

Improving expansion of pluripotent human embryonic stem cells in perfused bioreactors through oxygen control

scientific article published on 17 June 2010

Improving washing strategies of human mesenchymal stem cells using negative mode expanded bed chromatography.

scientific article published on 21 December 2015

In situ 2D fluorometry and chemometric monitoring of mammalian cell cultures.

scientific article published on March 2009

In vitro expansion of human cardiac progenitor cells: exploring 'omics tools for characterization of cell-based allogeneic products

scientific article published on 10 February 2016

In vitro response of the brown bullhead catfish (BB) and rainbow trout (RTG-2) cell lines to benzo[a]pyrene

scientific article

Industrial Large Scale of Suspension Culture of Insect Cells

article

Influence of environmental factors on lipase production by Lactobacillus plantarum.

scientific article published in February 1999

Insect cell culture medium supplementation with fetal bovine serum and bovine serum albumin: effects on baculovirus adsorption and infection kinetics

scientific article published on July 2002

Insect cells as a production platform of complex virus-like particles

scientific article published on February 2013

Integrated process optimization: lessons from retrovirus and virus-like particle production.

scientific article published in November 2002

Integrated strategy for the production of therapeutic retroviral vectors

scientific article published on 2 March 2011

Intracellular dynamics in rotavirus-like particles production: Evaluation of multigene and monocistronic infection strategies

article

Large scale production and downstream processing of a recombinant porcine parvovirus vaccine

scientific article published on 16 April 2002

Large-scale production and purification of VLP-based vaccines

scientific article published on July 2011

LentiPro26: novel stable cell lines for constitutive lentiviral vector production

scientific article

Manipulation of culture conditions for BHK cell growth inhibition by IRF-1 activation

scientific article published on February 2000

Measurement and prediction of distribution coefficients for wastewater aromatic solutes

scientific article published on 01 October 1983

Membrane-Based Approach for the Downstream Processing of Influenza Virus-Like Particles

scientific article published on 03 July 2019

Merging bioreactor technology with 3D hepatocyte-fibroblast culturing approaches: Improved in vitro models for toxicological applications.

scientific article published on 16 February 2011

Metabolic changes during cell growth inhibition by p27 overexpression

scientific article

Metabolic changes during cell growth inhibition by the IRF-1 system

Metabolic flux profiling of MDCK cells during growth and canine adenovirus vector production

scientific article published on 23 March 2016

Metabolic profiling of insect cell lines: Unveiling cell line determinants behind system's productivity

scientific article published on 20 November 2013

Metabolic responses of CHO cells to limitation of key amino acids

scientific article published on 28 July 2014

Metabolic responses to different glucose and glutamine levels in baby hamster kidney cell culture

scientific article published on May 1999

Metabolic shifts by nutrient manipulation in continuous cultures of BHK cells

scientific article

Metabolic signatures of GS-CHO cell clones associated with butyrate treatment and culture phase transition

scientific article published on 7 July 2013

Metabolically optimised BHK cell fed-batch cultures

scientific article published on June 2000

Metabolism of 3-(13)C-malate in primary cultures of mouse astrocytes

scientific article published on 01 September 2000

Methanol induction optimization for scFv antibody fragment production in Pichia pastoris.

scientific article

Microdosing of a Carbon-14 Labeled Protein in Healthy Volunteers Accurately Predicts Its Pharmacokinetics at Therapeutic Dosages

scientific article

Microencapsulation technology: a powerful tool for integrating expansion and cryopreservation of human embryonic stem cells

scientific article (publication date: 2011)

Model for carbon metabolism in biological phosphorus removal processes based on in vivo13C-NMR labelling experiments

article

Modeling electrostatic interactions of baculovirus vectors for ion-exchange process development

scientific article

Modeling protein binding and elution over a chromatographic surface probed by surface plasmon resonance

scientific article published in February 2010

Modeling retrovirus production for gene therapy. 1. Determination Of optimal bioreaction mode and harvest strategy

scientific article published in March 2000

Modeling retrovirus production for gene therapy. 2. Integrated optimization of bioreaction and downstream processing

scientific article

Modeling rotavirus-like particles production in a baculovirus expression vector system: Infection kinetics, baculovirus DNA replication, mRNA synthesis and protein production.

scientific article published on 10 January 2007

Modelling and optimization of a recombinant BHK-21 cultivation process using hybrid grey-box systems.

scientific article published on August 2005

NMR studies on energy metabolism of immobilized primary neurons and astrocytes during hypoxia, ischemia and hypoglycemia

scientific article published on 01 December 2000

Novel culture strategy for human stem cell proliferation and neuronal differentiation

scientific article published on December 2007

On the effect of thermodynamic equilibrium on the assembly efficiency of complex multi-layered virus-like particles (VLP): the case of rotavirus VLP.

scientific article

On-line detection of microbial contaminations in animal cell reactor cultures using an electronic nose device

scientific article

Operational patterns affecting lactic acid production in ultrafiltration cell recycle bioreactor

article

Optimization of the production of virus-like particles in insect cells

scientific article published on November 1998

Perfusion of 3D encapsulated hepatocytes-A synergistic effect enhancing long-term functionality in bioreactors

scientific article published on 01 January 2011

Predicting sporulation events in a bioreactor using an electronic nose

scientific article

Process development for the mass production of Ehrlichia ruminantium

scientific article published on 14 October 2005

Process development of a recombinant antibody/interleukin-2 fusion protein expressed in protein-free medium by BHK cells.

scientific article

Production and purification of lentiviral vectors generated in 293T suspension cells with baculoviral vectors

scientific article published on 20 January 2011

Production of oncolytic adenovirus and human mesenchymal stem cells in a single-use, Vertical-Wheel bioreactor system: Impact of bioreactor design on performance of microcarrier-based cell culture processes

scientific article published on 20 August 2015

Proteolytic activity in infected and noninfected insect cells: degradation of HIV-1 Pr55gag particles

scientific article published on October 1999

Proteomic analyses of Ehrlichia ruminantium highlight differential expression of MAP1-family proteins

scientific article

Pseudotyping retrovirus like particles vaccine candidates with Hepatitis C virus envelope protein E2 requires the cellular expression of CD81

scientific article published on 07 February 2019

Purification and characterization of an anti-apoptotic protein isolated from Lonomia obliqua hemolymph

scientific article published on January 2005

Purification of adenoviral vectors using expanded bed chromatography.

scientific article published on 2 November 2005

Purification of recombinant baculoviruses for gene therapy using membrane processes.

scientific article published on 2 April 2009

Purification of recombinant rotavirus VP7 glycoprotein for the study of in vitro rotavirus-like particles assembly

scientific article published on 7 September 2008

Purification of retroviral vectors for clinical application: biological implications and technological challenges

scientific article published on 31 July 2006

Quality control and analytical methods for baculovirus-based products

scientific article published on July 2011

Quantification of Ehrlichia ruminantium by real time PCR.

scientific article published on 24 February 2005

Quantitation of MLV-based retroviral vectors using real-time RT-PCR.

scientific article

Quantitative proteomics of Spodoptera frugiperda cells during growth and baculovirus infection.

scientific article

RMCE-based insect cell platform to produce membrane proteins captured on HIV-1 Gag virus-like particles

scientific article published on 16 November 2017

Rational design and optimization of downstream processes of virus particles for biopharmaceutical applications: current advances

scientific article published on 20 July 2011

Rational development of two flowthrough purification strategies for adenovirus type 5 and retro virus-like particles.

scientific article

Relationship between retroviral vector membrane and vector stability

scientific article published on May 2006

Relevance of an academic GMP Pan-European vector infra-structure (PEVI).

scientific article published on December 2010

Removal of envelope protein-free retroviral vectors by anion-exchange chromatography to improve product quality

scientific article

Repeated-batch cultures of baby hamster kidney cell aggregates in stirred vessels.

scientific article published on January 1994

Retrovirus producer cell line metabolism: implications on viral productivity.

scientific article published on 6 April 2006

Robust design of adenovirus purification by two-column, simulated moving-bed, size-exclusion chromatography.

scientific article

Rotavirus-like particle production: Simulation of protein production and particle assembly

article

Scalable culture systems using different cell lines for the production of Peste des Petits ruminants vaccine

scientific article

Scale-up of virus-like particles production: effects of sparging, agitation and bioreactor scale on cell growth, infection kinetics and productivity.

scientific article published on January 2004

Scaleable purification process for gene therapy retroviral vectors

scientific article published on April 2007

Screening anion-exchange chromatographic matrices for isolation of onco-retroviral vectors

scientific article published on 11 May 2006

Screening of novel excipients for improving the stability of retroviral and adenoviral vectors.

scientific article published on March 2006

Stabilization of gammaretroviral and lentiviral vectors: from production to gene transfer

scientific article

Stirred bioreactors for the expansion of adult pancreatic stem cells

scientific article published in November 2008

Stirred vessel cultures of rat brain cells aggregates: characterization of major metabolic pathways and cell population dynamics

scientific article

Stochastic simulation of protein expression in the baculovirus/insect cells system

article by António Roldão et al published January 2008 in Computers & Chemical Engineering

Strategies for improved stability of Peste des Petits Ruminants Vaccine

scientific article published in 2011

Strategies to modulate BHK cell proliferation by the regulation of IRF-1 expression

scientific article

Synchronous fluorescence spectroscopy as a novel tool to enable PAT applications in bioprocesses

scientific article published on 24 March 2011

Synthetic biology for bioengineering virus-like particle vaccines

scientific article published on 31 December 2018

Systems biotechnology of animal cells: the road to prediction ⬇️

scientific article published on April 26, 2012

Testing a new formulation for Peste des Petits Ruminants vaccine in Ethiopia

scientific article published on 11 March 2014

Tetraspanins displayed in retrovirus-derived virus-like particles and their immunogenicity.

scientific article published on 12 January 2016

The importance of 293 cell cycle phase on adenovirus vector production

article

The role of glia in neuronal recovery following anoxia: In vitro evidence of neuronal adaptation

scientific article

The use of recombinase mediated cassette exchange in retroviral vector producer cell lines: predictability and efficiency by transgene exchange

scientific article published on 9 March 2006

Toward system-level understanding of baculovirus-host cell interactions: from molecular fundamental studies to large-scale proteomics approaches

scientific article

Towards an extended functional hepatocyte in vitro culture

scientific article

Towards purification of adenoviral vectors based on membrane technology

scientific article published in November 2008

Towards real-time monitoring of therapeutic protein quality in mammalian cell processes.

scientific article published on 16 July 2014

Triple layered rotavirus VLP production: kinetics of vector replication, mRNA stability and recombinant protein production.

scientific article published on 14 July 2005

Two different serum-free media and osmolality effect upon human 293 cell growth and adenovirus production

scientific article published on November 2005

Two-dimensional versus three-dimensional culture systems: Effects on growth and productivity of BHK cells

article

Universal label-free in-process quantification of influenza virus-like particles

scientific article published on 17 May 2017

Virus production for clinical gene therapy

scientific article

Virus-like particle production at low multiplicities of infection with the baculovirus insect cell system.

scientific article

Virus-like particles in vaccine development.

scientific article published on October 2010

Paulina is supported by:

About Paulina

Help