List of works - James Murphy

1H, 13C and 15N resonance assignments of a highly-soluble murine interleukin-3 analogue with wild-type bioactivity

scientific article published on 20 February 2010

A Novel Functional Epitope Formed by Domains 1 and 4 of the Human Common β-Subunit Is Involved in Receptor Activation by Granulocyte Macrophage Colony-stimulating Factor and Interleukin 5

scientific article published on 12 January 2003

A RIPK2 inhibitor delays NOD signalling events yet prevents inflammatory cytokine production

scientific article

A bidentate Polycomb Repressive-Deubiquitinase complex is required for efficient activity on nucleosomes

scientific article published in Nature Communications

A convenient method for preparation of an engineered mouse interleukin-3 analog with high solubility and wild-type bioactivity

scientific article

A missense mutation in the MLKL brace region promotes lethal neonatal inflammation and hematopoietic dysfunction

scientific article published on 19 June 2020

A new isoform of interleukin-3 receptor {alpha} with novel differentiation activity and high affinity binding mode

scientific journal article

A robust methodology to subclassify pseudokinases based on their nucleotide-binding properties

scientific article

A tale of two domains - a structural perspective of the pseudokinase, MLKL.

scientific article published on 03 September 2015

Activated MLKL attenuates autophagy following its translocation to intracellular membranes

scientific article published on 28 February 2019

Activation of the pseudokinase MLKL unleashes the four-helix bundle domain to induce membrane localization and necroptotic cell death

scientific article

Active MLKL triggers the NLRP3 inflammasome in a cell-intrinsic manner.

scientific article published on 17 January 2017

An efficient high-throughput screening method for MYST family acetyltransferases, a new class of epigenetic drug targets

scientific article

An optimized SEC-SAXS system enabling high X-ray dose for rapid SAXS assessment with correlated UV measurements for biomolecular structure analysis

scholarly article

Analysis of the N-terminal region of human MLKL, as well as two distinct MLKL isoforms, reveals new insights into necroptotic cell death

scientific article

Ars Moriendi; the art of dying well - new insights into the molecular pathways of necroptotic cell death

scientific article published on 27 January 2014

BAK core dimers bind lipids and can be bridged by them

scientific article published on 14 September 2020

Bio-Zombie: the rise of pseudoenzymes in biology

scientific article

Characterization of Ligand Binding to Pseudokinases Using a Thermal Shift Assay

scientific article published in January 2017

Characterization of kinase target phosphorylation consensus motifs using peptide SPOT arrays

scientific article published on January 2009

Clarification of the role of N-glycans on the common beta-subunit of the human IL-3, IL-5 and GM-CSF receptors and the murine IL-3 beta-receptor in ligand-binding and receptor activation

scientific article

Conformational instability of the MARK3 UBA domain compromises ubiquitin recognition and promotes interaction with the adjacent kinase domain

scientific article

Conformational interconversion of MLKL and disengagement from RIPK3 precede cell death by necroptosis

scientific article published on 13 April 2021

Conformational switching of the pseudokinase domain promotes human MLKL tetramerization and cell death by necroptosis.

scientific article

Critical roles for c-Myb in lymphoid priming and early B-cell development

scientific article

Cryo-EM structure of an essential Plasmodium vivax invasion complex

scientific article published in Nature

Crystal structure of the hinge domain of Smchd1 reveals its dimerization mode and nucleic acid-binding residues

scientific article published on 16 June 2020

Crystal structure of the mouse interleukin-3 β-receptor: insights into interleukin-3 binding and receptor activation

scientific article

Dawn of the dead: protein pseudokinases signal new adventures in cell biology

scientific article published on August 2013

De novo mutations in SMCHD1 cause Bosma arhinia microphthalmia syndrome and abrogate nasal development

scientific article

Determination of the Plk4/Sak consensus phosphorylation motif using peptide spots arrays

scientific article

Development of NanoLuc-targeting protein degraders and a universal reporter system to benchmark tag-targeted degradation platforms

scientific article published on 19 April 2022

Distinct pseudokinase domain conformations underlie divergent activation mechanisms among vertebrate MLKL orthologues

scientific article published on 19 June 2020

Down the rabbit hole: Is necroptosis truly an innate response to infection?

scientific article published on 5 May 2017

Emerging concepts in pseudoenzyme classification, evolution, and signaling

scientific article published on 13 August 2019

Eph receptor signalling: from catalytic to non-catalytic functions

scientific article published on 12 August 2019

Epigenetic regulator Smchd1 functions as a tumor suppressor

scientific article published on 26 December 2012

Erratum: EspL is a bacterial cysteine protease effector that cleaves RHIM proteins to block necroptosis and inflammation

scientific article published on 13 February 2017

EspL is a bacterial cysteine protease effector that cleaves RHIM proteins to block necroptosis and inflammation

scientific article published on 13 January 2017

Evolution of Protein Quaternary Structure in Response to Selective Pressure for Increased Thermostability

scientific article

Evolutionary divergence of the necroptosis effector MLKL.

scientific article published on 12 February 2016

Exchange enhanced sensitivity gain for solvent-exchangeable protons in 2D 1H-15N heteronuclear correlation spectra acquired with band-selective pulses

scientific article

FSHD2- and BAMS-associated mutations confer opposing effects on SMCHD1 function

scientific article published on 10 May 2018

Flicking the molecular switch underlying MLKL-mediated necroptosis

scientific article published on 23 January 2015

Functional characterization of c-Mpl ectodomain mutations that underlie congenital amegakaryocytic thrombocytopenia

scientific article

Genome-wide binding and mechanistic analyses of Smchd1-mediated epigenetic regulation

scientific article published on 19 June 2015

Granulovirus PK-1 kinase activity relies on a side-to-side dimerization mode centered on the regulatory αC helix

scientific article published on 12 February 2021

HSP90 activity is required for MLKL oligomerisation and membrane translocation and the induction of necroptotic cell death.

scientific article published on 14 January 2016

High yield production of a soluble human interleukin-3 variant from E. coli with wild-type bioactivity and improved radiolabeling properties

scientific article

Human RIPK3 maintains MLKL in an inactive conformation prior to cell death by necroptosis

scientific article published on 22 November 2021

IL-3, IL-5, and GM-CSF signaling: crystal structure of the human beta-common receptor

scientific article published on January 2006

Identification of MLKL membrane translocation as a checkpoint in necroptotic cell death using Monobodies

scientific article published on 31 March 2020

Identification of a second binding site on the TRIM25 B30.2 domain.

scientific article published on 19 December 2017

In vitro JAK kinase activity and inhibition assays

scientific article

Insane in the membrane: a structural perspective of MLKL function in necroptosis

scientific article published on 21 December 2016

Insights into the evolution of divergent nucleotide-binding mechanisms among pseudokinases revealed by crystal structures of human and mouse MLKL

scientific article

Interleukin-3 binding to the murine betaIL-3 and human betac receptors involves functional epitopes formed by domains 1 and 4 of different protein chains

scientific article published in April 2004

Laser-mediated rupture of chlamydial inclusions triggers pathogen egress and host cell necrosis

scientific article

Live and let die: insights into pseudoenzyme mechanisms from structure

scientific article published on 5 August 2017

Long-range chromatin interactions on the inactive X and at Hox clusters are regulated by the non-canonical SMC protein Smchd1

Lymphotoxin α induces apoptosis, necroptosis and inflammatory signals with the same potency as tumour necrosis factor.

scientific article

MK2 Phosphorylates RIPK1 to Prevent TNF-Induced Cell Death

scientific article

MLKL trafficking and accumulation at the plasma membrane control the kinetics and threshold for necroptosis

scientific article published on 19 June 2020

Mechanism of NanR gene repression and allosteric induction of bacterial sialic acid metabolism

scientific article published on 31 March 2021

Mechanism of NanR gene repression and allosteric induction of bacterial sialic acid metabolism

Mechanistic insights into activation and SOCS3-mediated inhibition of myeloproliferative neoplasm-associated JAK2 mutants from biochemical and structural analyses

scientific article

Mitogen-activated Tasmanian devil blood mononuclear cells kill devil facial tumour disease cells

scientific article published on 19 April 2016

Molecular Mechanism of CCAAT-Enhancer Binding Protein Recruitment by the TRIB1 Pseudokinase

scientific article

Monosodium Urate Crystals Generate Nuclease-Resistant Neutrophil Extracellular Traps via a Distinct Molecular Pathway.

scientific article

More to life than death: molecular determinants of necroptotic and non-necroptotic RIP3 kinase signaling

scientific article

Murine interleukin-3: structure, dynamics, and conformational heterogeneity in solution

scientific article

Necroptosis and ferroptosis are alternative cell death pathways that operate in acute kidney failure

scientific article published on 27 May 2017

Necroptosis induced by RIPK3 requires MLKL but not Drp1.

scientific article published on 27 February 2014

Necroptosis is dispensable for motor neuron degeneration in a mouse model of ALS

scientific article published on 19 November 2019

Necroptosis is dispensable for the development of inflammation-associated or sporadic colon cancer in mice

scientific article published on 23 November 2020

Necroptosis signalling is tuned by phosphorylation of MLKL residues outside the pseudokinase domain activation loop

scientific article published on 17 August 2015

PD-L1 Is Not Constitutively Expressed on Tasmanian Devil Facial Tumor Cells but Is Strongly Upregulated in Response to IFN-γ and Can Be Expressed in the Tumor Microenvironment

scientific article published on 09 December 2016

Phosphorylation by Aurora B kinase regulates caspase-2 activity and function

scientific article published on 18 August 2020

Point mutation in the gene encoding p300 suppresses thrombocytopenia in Mpl-/- mice

scientific journal article

Post-translational control of RIPK3 and MLKL mediated necroptotic cell death

scientific article published on 19 November 2015

Potent inhibition of Necroptosis by simultaneously targeting multiple effectors of the pathway

scientific article published on 09 September 2020

RIPK1 regulates RIPK3-MLKL-driven systemic inflammation and emergency hematopoiesis

scientific article published on 8 May 2014

RIPK1- and RIPK3-induced cell death mode is determined by target availability

scientific article

Rapid identification of linear protein domain binding motifs using peptide SPOT arrays

scientific article published on January 2009

Regression of devil facial tumour disease following immunotherapy in immunised Tasmanian devils

scientific article published on 9 March 2017

Regulation of Janus kinases by SOCS proteins

scientific article published on August 2013

Relating SMCHD1 structure to its function in epigenetic silencing

scientific article published on 11 August 2020

SMCHD1 is involved in de novo methylation of the DUX4-encoding D4Z4 macrosatellite

scientific article published on 01 April 2019

SMCHD1's ubiquitin-like domain is required for N-terminal dimerization and chromatin localization

scientific article published on 01 July 2021

SOCS3 binds specific receptor–JAK complexes to control cytokine signaling by direct kinase inhibition

scientific article

Screening for PTB Domain Binding Partners and Ligand Specificity Using Proteome-Derived NPXY Peptide Arrays

Screening for PTB domain binding partners and ligand specificity using proteome-derived NPXY peptide arrays

scientific article published on 18 September 2006

Smchd1 Targeting to the Inactive X Is Dependent on the Xist-HnrnpK-PRC1 Pathway

scientific article published on 01 November 2018

Smchd1 regulates long-range chromatin interactions on the inactive X chromosome and at Hox clusters

scientific article published on 20 August 2018

Structural basis of autoregulatory scaffolding by apoptosis signal-regulating kinase 1.

scientific article published on 27 February 2017

Structural studies of FF domains of the transcription factor CA150 provide insights into the organization of FF domain tandem arrays

scientific article

Structurally conserved erythrocyte-binding domain in Plasmodium provides a versatile scaffold for alternate receptor engagement

scientific article

Structure and Functional Characterization of the Conserved JAK Interaction Region in the Intrinsically Disordered N-Terminus of SOCS5

scientific article

Structure of SgK223 pseudokinase reveals novel mechanisms of homotypic and heterotypic association

scientific article published on 27 October 2017

Structure of the complete extracellular domain of the common beta subunit of the human GM-CSF, IL-3, and IL-5 receptors reveals a novel dimer configuration

scientific article

Structure-based mechanism of preferential complex formation by apoptosis signal-regulating kinases

scientific article published on 10 March 2020

Suppression of cytokine signaling by SOCS3: characterization of the mode of inhibition and the basis of its specificity

scientific article published in February 2012

Suppressor of Cytokine Signaling (SOCS) 5 utilises distinct domains for regulation of JAK1 and interaction with the adaptor protein Shc-1.

scientific article

Synthesis of Functionalized Piperidinones

scientific article published on 01 March 2003

TNF can activate RIPK3 and cause programmed necrosis in the absence of RIPK1.

scientific article published on 17 January 2013

TNFR1-dependent cell death drives inflammation in Sharpin-deficient mice.

scientific article

Techniques to examine nucleotide binding by pseudokinases

scientific article published on August 2013

The Epigenetic Regulator SMCHD1 in Development and Disease

scientific article published on 17 February 2017

The Highway to Hell: A RIP Kinase-Directed Shortcut to Inflammatory Cytokine Production

scientific article published on July 2016

The Ig-like domain of human GM-CSF receptor alpha plays a critical role in cytokine binding and receptor activation

scientific article

The Negative Regulation of JAK/STAT Signaling

The PEAK family of pseudokinases, their role in cell signalling and cancer

scientific article published on 09 October 2019

The Pyroptotic Cell Death Effector Gasdermin D Is Activated by Gout-Associated Uric Acid Crystals but Is Dispensable for Cell Death and IL-1β Release

scientific article published on 17 June 2019

The Structural Basis of Necroptotic Cell Death Signaling

scientific article published on 30 November 2018

The anticonvulsive Phenhydan® suppresses extrinsic cell death

scientific article published on 15 November 2018

The brace helices of MLKL mediate interdomain communication and oligomerisation to regulate cell death by necroptosis

scientific article published on 14 February 2018

The epigenetic regulator Smchd1 contains a functional GHKL-type ATPase domain.

scientific article published on 8 April 2016

The evolving world of pseudoenzymes: proteins, prejudice and zombies

scientific article

The hinge domain of the epigenetic repressor Smchd1 adopts an unconventional homodimeric configuration

scientific article published on 05 January 2016

The intracellular domains of the EphB6 and EphA10 receptor tyrosine pseudokinases function as dynamic signalling hubs

scientific article published on 01 September 2021

The long-awaited structure of HIPK2

scientific article published on 01 September 2019

The molecular basis of JAK/STAT inhibition by SOCS1.

scientific article published on 19 April 2018

The molecular regulation of Janus kinase (JAK) activation

scientific article

The pseudokinase MLKL mediates necroptosis via a molecular switch mechanism

scientific article

The pseudokinase MLKL mediates programmed hepatocellular necrosis independently of RIPK3 during hepatitis

scientific article published on 18 October 2016

The role of interchain heterodisulfide formation in activation of the human common beta and mouse betaIL-3 receptors

scientific article

The secret life of kinases: insights into non-catalytic signalling functions from pseudokinases.

scientific article

The ubiquitylation of IL-1β limits its cleavage by caspase-1 and targets it for proteasomal degradation

scientific article published on 11 May 2021

Transferrin receptor 1 is a reticulocyte-specific receptor for Plasmodium vivax.

scientific article

Two modes of beta-receptor recognition are mediated by distinct epitopes on mouse and human interleukin-3.

scientific article

Ubiquitylation of MLKL at lysine 219 positively regulates necroptosis-induced tissue injury and pathogen clearance

scientific article published on 07 June 2021

Viral MLKL Homologs Subvert Necroptotic Cell Death by Sequestering Cellular RIPK3

scientific article published on 01 September 2019

cIAPs and XIAP regulate myelopoiesis through cytokine production in an RIPK1- and RIPK3-dependent manner

scientific article published on 04 February 2014

List of works by James Murphy

1H, 13C and 15N resonance assignments of a highly-soluble murine interleukin-3 analogue with wild-type bioactivity

A Novel Functional Epitope Formed by Domains 1 and 4 of the Human Common β-Subunit Is Involved in Receptor Activation by Granulocyte Macrophage Colony-stimulating Factor and Interleukin 5

A RIPK2 inhibitor delays NOD signalling events yet prevents inflammatory cytokine production

A bidentate Polycomb Repressive-Deubiquitinase complex is required for efficient activity on nucleosomes

A convenient method for preparation of an engineered mouse interleukin-3 analog with high solubility and wild-type bioactivity

A missense mutation in the MLKL brace region promotes lethal neonatal inflammation and hematopoietic dysfunction

A new isoform of interleukin-3 receptor {alpha} with novel differentiation activity and high affinity binding mode

A robust methodology to subclassify pseudokinases based on their nucleotide-binding properties

A tale of two domains - a structural perspective of the pseudokinase, MLKL.

Activated MLKL attenuates autophagy following its translocation to intracellular membranes

Activation of the pseudokinase MLKL unleashes the four-helix bundle domain to induce membrane localization and necroptotic cell death

Active MLKL triggers the NLRP3 inflammasome in a cell-intrinsic manner.

An efficient high-throughput screening method for MYST family acetyltransferases, a new class of epigenetic drug targets

An optimized SEC-SAXS system enabling high X-ray dose for rapid SAXS assessment with correlated UV measurements for biomolecular structure analysis

Analysis of the N-terminal region of human MLKL, as well as two distinct MLKL isoforms, reveals new insights into necroptotic cell death

Ars Moriendi; the art of dying well - new insights into the molecular pathways of necroptotic cell death

BAK core dimers bind lipids and can be bridged by them

Bio-Zombie: the rise of pseudoenzymes in biology

Characterization of Ligand Binding to Pseudokinases Using a Thermal Shift Assay

Characterization of kinase target phosphorylation consensus motifs using peptide SPOT arrays

Clarification of the role of N-glycans on the common beta-subunit of the human IL-3, IL-5 and GM-CSF receptors and the murine IL-3 beta-receptor in ligand-binding and receptor activation

Conformational instability of the MARK3 UBA domain compromises ubiquitin recognition and promotes interaction with the adjacent kinase domain

Conformational interconversion of MLKL and disengagement from RIPK3 precede cell death by necroptosis

Conformational switching of the pseudokinase domain promotes human MLKL tetramerization and cell death by necroptosis.

Critical roles for c-Myb in lymphoid priming and early B-cell development

Cryo-EM structure of an essential Plasmodium vivax invasion complex

Crystal structure of the hinge domain of Smchd1 reveals its dimerization mode and nucleic acid-binding residues

Crystal structure of the mouse interleukin-3 β-receptor: insights into interleukin-3 binding and receptor activation

Dawn of the dead: protein pseudokinases signal new adventures in cell biology

De novo mutations in SMCHD1 cause Bosma arhinia microphthalmia syndrome and abrogate nasal development

Determination of the Plk4/Sak consensus phosphorylation motif using peptide spots arrays

Development of NanoLuc-targeting protein degraders and a universal reporter system to benchmark tag-targeted degradation platforms

Distinct pseudokinase domain conformations underlie divergent activation mechanisms among vertebrate MLKL orthologues

Down the rabbit hole: Is necroptosis truly an innate response to infection?

Emerging concepts in pseudoenzyme classification, evolution, and signaling

Eph receptor signalling: from catalytic to non-catalytic functions

Epigenetic regulator Smchd1 functions as a tumor suppressor

Erratum: EspL is a bacterial cysteine protease effector that cleaves RHIM proteins to block necroptosis and inflammation

EspL is a bacterial cysteine protease effector that cleaves RHIM proteins to block necroptosis and inflammation

Evolution of Protein Quaternary Structure in Response to Selective Pressure for Increased Thermostability

Evolutionary divergence of the necroptosis effector MLKL.

Exchange enhanced sensitivity gain for solvent-exchangeable protons in 2D 1H-15N heteronuclear correlation spectra acquired with band-selective pulses

FSHD2- and BAMS-associated mutations confer opposing effects on SMCHD1 function

Flicking the molecular switch underlying MLKL-mediated necroptosis

Functional characterization of c-Mpl ectodomain mutations that underlie congenital amegakaryocytic thrombocytopenia

Genome-wide binding and mechanistic analyses of Smchd1-mediated epigenetic regulation

Granulovirus PK-1 kinase activity relies on a side-to-side dimerization mode centered on the regulatory αC helix

HSP90 activity is required for MLKL oligomerisation and membrane translocation and the induction of necroptotic cell death.

High yield production of a soluble human interleukin-3 variant from E. coli with wild-type bioactivity and improved radiolabeling properties

Human RIPK3 maintains MLKL in an inactive conformation prior to cell death by necroptosis

IL-3, IL-5, and GM-CSF signaling: crystal structure of the human beta-common receptor

Identification of MLKL membrane translocation as a checkpoint in necroptotic cell death using Monobodies

Identification of a second binding site on the TRIM25 B30.2 domain.

In vitro JAK kinase activity and inhibition assays

Insane in the membrane: a structural perspective of MLKL function in necroptosis

Insights into the evolution of divergent nucleotide-binding mechanisms among pseudokinases revealed by crystal structures of human and mouse MLKL

Interleukin-3 binding to the murine betaIL-3 and human betac receptors involves functional epitopes formed by domains 1 and 4 of different protein chains

Laser-mediated rupture of chlamydial inclusions triggers pathogen egress and host cell necrosis

Live and let die: insights into pseudoenzyme mechanisms from structure

Long-range chromatin interactions on the inactive X and at Hox clusters are regulated by the non-canonical SMC protein Smchd1

Lymphotoxin α induces apoptosis, necroptosis and inflammatory signals with the same potency as tumour necrosis factor.

MK2 Phosphorylates RIPK1 to Prevent TNF-Induced Cell Death

MLKL trafficking and accumulation at the plasma membrane control the kinetics and threshold for necroptosis

Mechanism of NanR gene repression and allosteric induction of bacterial sialic acid metabolism

Mechanism of NanR gene repression and allosteric induction of bacterial sialic acid metabolism

Mechanistic insights into activation and SOCS3-mediated inhibition of myeloproliferative neoplasm-associated JAK2 mutants from biochemical and structural analyses

Mitogen-activated Tasmanian devil blood mononuclear cells kill devil facial tumour disease cells

Molecular Mechanism of CCAAT-Enhancer Binding Protein Recruitment by the TRIB1 Pseudokinase

Monosodium Urate Crystals Generate Nuclease-Resistant Neutrophil Extracellular Traps via a Distinct Molecular Pathway.

More to life than death: molecular determinants of necroptotic and non-necroptotic RIP3 kinase signaling

Murine interleukin-3: structure, dynamics, and conformational heterogeneity in solution

Necroptosis and ferroptosis are alternative cell death pathways that operate in acute kidney failure

Necroptosis induced by RIPK3 requires MLKL but not Drp1.

Necroptosis is dispensable for motor neuron degeneration in a mouse model of ALS

Necroptosis is dispensable for the development of inflammation-associated or sporadic colon cancer in mice

Necroptosis signalling is tuned by phosphorylation of MLKL residues outside the pseudokinase domain activation loop

PD-L1 Is Not Constitutively Expressed on Tasmanian Devil Facial Tumor Cells but Is Strongly Upregulated in Response to IFN-γ and Can Be Expressed in the Tumor Microenvironment

Phosphorylation by Aurora B kinase regulates caspase-2 activity and function

Point mutation in the gene encoding p300 suppresses thrombocytopenia in Mpl-/- mice