List of works by Florian Hollfelder

A Shorter Route to Antibody Binders via Quantitative in vitro Bead-Display Screening and Consensus Analysis

scientific article

A Titratable Cell Lysis-on-Demand System for Droplet-Compartmentalized Ultrahigh-Throughput Screening in Functional Metagenomics and Directed Evolution

scientific article published on 14 July 2021

A Trojan horse transition state analogue generated by MgF3- formation in an enzyme active site.

scholarly article

A covalent chemical genotype-phenotype linkage for in vitro protein evolution

scientific article

A double droplet trap system for studying mass transport across a droplet-droplet interface

scientific article published on 16 February 2010

A fully unsupervised compartment-on-demand platform for precise nanoliter assays of time-dependent steady-state enzyme kinetics and inhibition.

scientific article

A method to quantify FRET stoichiometry with phasor plot analysis and acceptor lifetime ingrowth

scientific article

A new member of the alkaline phosphatase superfamily with a formylglycine nucleophile: structural and kinetic characterisation of a phosphonate monoester hydrolase/phosphodiesterase from Rhizobium leguminosarum

scientific article

A simple method to evaluate the biochemical compatibility of oil/surfactant mixtures for experiments in microdroplets

scientific article published in October 2012

A single mutation in the core domain of the lac repressor reduces leakiness

scientific article published on 8 July 2013

Accessing unexplored regions of sequence space in directed enzyme evolution via insertion/deletion mutagenesis

scientific article published on 10 July 2020

An efficient method to assemble linear DNA templates for in vitro screening and selection systems

scientific article published on 17 July 2009

An efficient, multiply promiscuous hydrolase in the alkaline phosphatase superfamily

scientific article

An experimental framework for improved selection of binding proteins using SNAP display

scientific article published on 20 January 2014

An integrated cell culture lab on a chip: modular microdevices for cultivation of mammalian cells and delivery into microfluidic microdroplets

scientific article

An integrated device for monitoring time-dependent in vitro expression from single genes in picolitre droplets.

scientific article published in February 2008

An optimized ATP/PP(i)-exchange assay in 96-well format for screening of adenylation domains for applications in combinatorial biosynthesis

scientific article published on 01 February 2007

Anionic charge is prioritized over geometry in aluminum and magnesium fluoride transition state analogs of phosphoryl transfer enzymes

scientific article published on 5 March 2008

Assembling linear DNA templates for in vitro transcription and translation.

scientific article

Balancing Specificity and Promiscuity in Enzyme Evolution: Multidimensional Activity Transitions in the Alkaline Phosphatase Superfamily

scientific article published on 20 December 2018

Bioinspired genotype-phenotype linkages: mimicking cellular compartmentalization for the engineering of functional proteins.

scientific article published on August 2015

Catalysis of the Kemp elimination by antibodies elicited against a cationic hapten

scientific article published in 1997

Combinatorial Screening Identifies Novel Promiscuous Matrix Metalloproteinase Activities that Lead to Inhibition of the Therapeutic Target IL-13.

scientific article published on 3 November 2015

Combinatorial Synthesis of Structurally Diverse Triazole-Bridged Flavonoid Dimers and Trimers.

scientific article

Combining medium effects and cofactor catalysis: metal-coordinated synzymes accelerate phosphate transfer by 10(8).

scientific article published in November 2009

Continuous-flow polymerase chain reaction of single-copy DNA in microfluidic microdroplets

scientific article published in January 2009

Controlled Oil/Water Partitioning of Hydrophobic Substrates Extending the Bioanalytical Applications of Droplet-Based Microfluidics

scientific article published on 10 July 2019

Controlling the contents of microdroplets by exploiting the permeability of PDMS.

scientific article published on 7 February 2011

Controlling the retention of small molecules in emulsion microdroplets for use in cell-based assays

scientific article published in April 2009

Cross-talk between histone modifications in response to histone deacetylase inhibitors: MLL4 links histone H3 acetylation and histone H3K4 methylation

scientific article

Deep learning guided image-based droplet sorting for on-demand selection and analysis of single cells and 3D cell cultures

scientific article published on 01 March 2020

Development of quantitative cell-based enzyme assays in microdroplets

scientific article published on 10 April 2008

Directed evolution of a gatekeeper domain in nonribosomal peptide synthesis

scientific article published on 01 October 2011

Directed evolution of a histone acetyltransferase--enhancing thermostability, whilst maintaining catalytic activity and substrate specificity.

scientific article

Directed evolution of anti-HER2 DARPins by SNAP display reveals stability/function trade-offs in the selection process

scientific article

Divergent synthesis of biflavonoids yields novel inhibitors of the aggregation of amyloid β (1-42).

scientific article

Droplet-based screening of phosphate transfer catalysis reveals how epistasis shapes MAP kinase interactions with substrates

scientific article published on 11 February 2022

Droplets as reaction compartments for protein nanotechnology.

scientific article published on January 2013

Efficient Transfection of siRNA by Peptide Dendrimer-Lipid Conjugates.

scientific article published on 9 November 2016

Efficient catalytic promiscuity for chemically distinct reactions

scientific article published on 01 January 2009

Efficient catalytic promiscuity in an enzyme superfamily: an arylsulfatase shows a rate acceleration of 10(13) for phosphate monoester hydrolysis

scientific journal article

Efficient, crosswise catalytic promiscuity among enzymes that catalyze phosphoryl transfer

scientific article

Embryo model completes gastrulation to neurulation and organogenesis

scientific article published in 2022

Engineering the protein dynamics of an ancestral luciferase

scientific article published on 14 June 2021

Enzyme engineering in biomimetic compartments

scientific article published on 23 August 2015

Enzymes under the nanoscope

scientific article published in Nature

European Symposium of Bio-Organic Chemistry 2003 (ESBOC): the evolution of catalysis

scientific article published on 01 February 2004

Evolution of enzyme catalysts caged in biomimetic gel-shell beads

scientific article

Evolutionary repurposing of a sulfatase: A new Michaelis complex leads to efficient transition state charge offset

article published in the Proceedings of the National Academy of Sciences of the United States of America

Exploring sequence space in search of functional enzymes using microfluidic droplets

scientific article

Flexibility and reactivity in promiscuous enzymes

scientific article published on 29 January 2013

Fluid phase endocytosis contributes to transfection of DNA by PEI-25.

scientific article published on 16 June 2009

From fascination to function

scientific article published on 20 July 2011

Functional Trade-Offs in Promiscuous Enzymes Cannot Be Explained by Intrinsic Mutational Robustness of the Native Activity

scientific article

Global fitness landscapes of the Shine-Dalgarno sequence

scientific article published on 18 May 2020

Handicap-Recover Evolution Leads to a Chemically Versatile, Nucleophile-Permissive Protease

scientific article

High-Throughput, Lysis-Free Screening for Sulfatase Activity Using Escherichia coli Autodisplay in Microdroplets

scientific article published on 04 December 2019

Hitting a moving target?--Understanding how conformational diversity impacts enzymatic catalysis

scientific article published on 01 October 2010

Impaired transition state complementarity in the hydrolysis of O-arylphosphorothioates by protein-tyrosine phosphatases.

scientific article

Improved RAD51 binders through motif shuffling based on the modularity of BRC repeats

scientific article published on 01 November 2021

In vitro affinity screening of protein and peptide binders by megavalent bead surface display

scientific article

In vitro characterization of DNA gyrase inhibition by microcin B17 analogs with altered bisheterocyclic sites

scientific article published on 26 June 2001

In vitro evolution of antibody affinity via insertional scanning mutagenesis of an entire antibody variable region

scientific article published on 16 October 2020

Interfacing microwells with nanoliter compartments: a sampler generating high-resolution concentration gradients for quantitative biochemical analyses in droplets.

scientific article published on 12 December 2014

Investigating host-microbiome interactions by droplet based microfluidics

scientific article published on 01 October 2020

Isothermal DNA amplification using the T4 replisome: circular nicking endonuclease-dependent amplification and primase-based whole-genome amplification

scientific article

Just (protein) engineering?

scientific article published on 22 July 2013

Kinetic analysis of beta-phosphoglucomutase and its inhibition by magnesium fluoride

scientific article published in February 2009

Kinetic and computational evidence for an intermediate in the hydrolysis of sulfonate esters

scientific article published in October 2012

Long-Term Perfusion Culture of Monoclonal Embryonic Stem Cells in 3D Hydrogel Beads for Continuous Optical Analysis of Differentiation

scientific article published on 20 December 2018

Mapping catalytic promiscuity in the alkaline phosphatase superfamily

Mapping the limits of substrate specificity of the adenylation domain of TycA.

scientific article

Measuring Fast and Slow Enzyme Kinetics in Stationary Droplets

scientific article published on 2 November 2015

Mechanism and Catalytic Promiscuity: Emerging Mechanistic Principles for Identification and Manipulation of Catalytically Promiscuous Enzymes

Microdroplets in microfluidics: an evolving platform for discoveries in chemistry and biology

scientific article

Microdroplets: a sea of applications?

scientific article published on 24 June 2008

Microfluidic droplets: new integrated workflows for biological experiments

scientific article published on 24 September 2010

Microfluidic platform for 3D cell culture with live imaging and clone retrieval

scientific article published on 23 June 2020

Molecular recognition of DNA by rigid [N]-polynorbornane-derived bifunctional intercalators: synthesis and evaluation of their binding properties

scientific article

Monitoring a reaction at submillisecond resolution in picoliter volumes.

scientific article published on 18 January 2011

Multiplexed Affinity Characterization of Protein Binders Directly from a Crude Cell Lysate by Covalent Capture on Suspension Bead Arrays

scientific article published on 04 January 2021

Mutations in Mll2, an H3K4 methyltransferase, result in insulin resistance and impaired glucose tolerance in mice

scientific article (publication date: 2013)

New genotype-phenotype linkages for directed evolution of functional proteins

scientific article

Nonspecific catalysis by protein surfaces

scientific article published on 01 January 2000

Novel Peptide-dendrimer/Lipid/Oligonucleotide Ternary Complexes for Efficient Cellular Uptake and Improved Splice-Switching Activity

scientific article published on 04 September 2018

Off-the-shelf proteins that rival tailor-made antibodies as catalysts.

scientific article

On synergy between ultrahigh throughput screening and machine learning in biocatalyst engineering

scientific article published in 2024

On the magnitude and specificity of medium effects in enzyme-like catalysts for proton transfer

scientific article published on 01 August 2001

One in a million: flow cytometric sorting of single cell-lysate assays in monodisperse picolitre double emulsion droplets for directed evolution

scientific article

One-Pot Deracemization of sec-Alcohols: Enantioconvergent Enzymatic Hydrolysis of Alkyl Sulfates Using Stereocomplementary Sulfatases

scientific article published on 10 February 2013

One-pot deracemization of sec-alcohols: enantioconvergent enzymatic hydrolysis of alkyl sulfates using stereocomplementary sulfatases

scientific article

Peptide Dendrimer-Lipid Conjugates as DNA and siRNA Transfection Reagents: Role of Charge Distribution Across Generations

scientific article published in April 2017

Peptide dendrimer/lipid hybrid systems are efficient DNA transfection reagents: structure--activity relationships highlight the role of charge distribution across dendrimer generations

scientific article published on 17 May 2013

Picoliter cell lysate assays in microfluidic droplet compartments for directed enzyme evolution.

scientific article published in August 2012

Plasmodium dihydrofolate reductase is a second enzyme target for the antimalarial action of triclosan

scientific article published on 18 January 2018

Polyethylene imine derivatives ('synzymes') accelerate phosphate transfer in the absence of metal

scientific article published on 26 May 2007

Quantitative Affinity Determination by Fluorescence Anisotropy Measurements of Individual Nanoliter Droplets

scientific article

Quantitative detection of protein expression in single cells using droplet microfluidics

scientific article published on 26 January 2007

Relating chemical and biological diversity space: a tunable system for efficient gene transfection

scientific article

Removal of background signals from fluorescence thermometry measurements in PDMS microchannels using fluorescence lifetime imaging.

scientific article published on 24 September 2009

Reverse evolution leads to genotypic incompatibility despite functional and active site convergence

scientific article

SNAP dendrimers: multivalent protein display on dendrimer-like DNA for directed evolution.

scientific article published on 21 July 2011

SNAP display - an in vitro method for the selection of protein binders.

scientific article published on January 2013

SNAP display: in vitro protein evolution in microdroplets

scientific article

Self-Organization of Mouse Stem Cells into an Extended Potential Blastoid

scientific article published on 01 December 2019

Simultaneous determination of gene expression and enzymatic activity in individual bacterial cells in microdroplet compartments

scientific article

Simultaneous measurement of reactions in microdroplets filled by concentration gradients

scientific article

Single-cell activity screening in microfluidic droplets

scientific article published on 08 August 2019

Specificity Effects of Amino Acid Substitutions in Promiscuous Hydrolases: Context-Dependence of Catalytic Residue Contributions to Local Fitness Landscapes in Nearby Sequence Space

scientific article

Split & mix assembly of DNA libraries for ultrahigh throughput on-bead screening of functional proteins

scientific article published on 08 May 2020

Static microdroplet arrays: a microfluidic device for droplet trapping, incubation and release for enzymatic and cell-based assays

scientific article

Stem cell-derived synthetic embryos self-assemble by exploiting cadherin codes and cortical tension

scientific article published in September 2022

Structural and Mechanistic Analysis of the Choline Sulfatase from Sinorhizobium melliloti: A Class I Sulfatase Specific for an Alkyl Sulfate Ester

scientific article published on 16 February 2018

The antibiotic microcin B17 is a DNA gyrase poison: characterisation of the mode of inhibition

scientific article published in April 2001

The human histone acetyltransferase P/CAF is a promiscuous histone propionyltransferase

scientific article

The nature of the transition state for enzyme-catalyzed phosphoryl transfer. Hydrolysis of O-aryl phosphorothioates by alkaline phosphatase

scientific article published in September 1995

The potential of microfluidic water-in-oil droplets in experimental biology

scientific article

The role of protein dynamics in the evolution of new enzyme function

scientific article

Transition-State Interactions in a Promiscuous Enzyme: Sulfate and Phosphate Monoester Hydrolysis by Pseudomonas aeruginosa Arylsulfatase

scientific article published on 27 February 2019

UMI-linked consensus sequencing enables phylogenetic analysis of directed evolution

scientific article published on 26 November 2020

USER friendly DNA recombination (USERec): a simple and flexible near homology-independent method for gene library construction

scientific article

USER friendly DNA recombination (USERec): gene library construction requiring minimal sequence homology.

scientific article

Ultrahigh throughput screening for enzyme function in droplets

scientific article published on 24 July 2020

Ultrahigh-Throughput Screening of Single-Cell Lysates for Directed Evolution and Functional Metagenomics

scientific article published in January 2018

Ultrahigh-throughput discovery of promiscuous enzymes by picodroplet functional metagenomics

scientific article

Ultrahigh-throughput-directed enzyme evolution by absorbance-activated droplet sorting (AADS).

scientific article

Ultrarapid generation of femtoliter microfluidic droplets for single-molecule-counting immunoassays

scientific article published on 8 July 2013

Vesicles accelerate proton transfer from carbon up to 850-fold

scientific article published on 01 January 2000

What makes an enzyme promiscuous?

scientific article published on 18 January 2010

peri-Dimethylamino substituent effects on proton transfer at carbon in α-naphthylacetateesters: a model for mandelate racemase

article

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