List of works - Mark D. Szczelkun

A RecB-family nuclease motif in the Type I restriction endonuclease EcoR124I

scientific article published on 29 May 2008

A new detection system for extremely small vertically mounted cantilevers

scientific article published on 12 August 2008

An Mrr-family nuclease motif in the single polypeptide restriction-modification enzyme LlaGI.

scientific article published on November 2009

CgII cleaves DNA using a mechanism distinct from other ATP-dependent restriction endonucleases

scientific article published on August 2017

Characterization of the Type III restriction endonuclease PstII from Providencia stuartii

scientific article published on 01 December 2019

Characterization of the Type III restriction endonuclease PstII from Providencia stuartii.

scientific article

ClpXP protease targets long-lived DNA translocation states of a helicase-like motor to cause restriction alleviation

scientific article published on 26 September 2014

Continuous assays for DNA translocation using fluorescent triplex dissociation: application to type I restriction endonucleases.

scientific article

Controlling the motor activity of a transcription-repair coupling factor: autoinhibition and the role of RNA polymerase

scientific article published on 28 February 2007

DNA cleavage and methylation specificity of the single polypeptide restriction-modification enzyme LlaGI.

scientific article published on November 2009

DNA cleavage by CgII and NgoAVII requires interaction between N- and R-proteins and extensive nucleotide hydrolysis

scientific article published on 27 November 2014

DNA cleavage by Type ISP Restriction-Modification enzymes is initially targeted to the 3'-5' strand

scientific article published on 5 December 2012

DNA cleavage site selection by Type III restriction enzymes provides evidence for head-on protein collisions following 1D bidirectional motion.

scientific article

Direct and random routing of a molecular motor protein at a DNA junction

scientific article published on 26 August 2006

Direct observation of R-loop formation by single RNA-guided Cas9 and Cascade effector complexes.

scientific article

Dissociation from DNA of Type III Restriction-Modification enzymes during helicase-dependent motion and following endonuclease activity

scientific article published on 20 April 2012

Dynamics of initiation, termination and reinitiation of DNA translocation by the motor protein EcoR124I

scientific article

Hexameric assembly of the AAA+ protein McrB is necessary for GTPase activity

How to Build a DNA Unwinding Machine ⬇️

scientific article published on July 3, 2012

Length heterogeneity at conserved sequence block 2 in human mitochondrial DNA acts as a rheostat for RNA polymerase POLRMT activity

scientific article published on 19 July 2016

Maintaining a sense of direction during long-range communication on DNA.

scientific article published on April 2010

Mapping DNA cleavage by the Type ISP restriction-modification enzymes following long-range communication between DNA sites in different orientations

scientific article

Mitochondrial import, health and mtDNA copy number variability using type II and type V CRISPR effectors

scientific article published on 25 August 2020

Motor step size and ATP coupling efficiency of the dsDNA translocase EcoR124I.

scientific article

Re-evaluating the kinetics of ATP hydrolysis during initiation of DNA sliding by Type III restriction enzymes

scientific article published on 3 November 2015

Recycling of protein subunits during DNA translocation and cleavage by Type I restriction-modification enzymes

scientific article published on 28 June 2011

Repercussions of DNA tracking by the type IC restriction endonuclease EcoR124I on linear, circular and catenated substrates.

scientific article

Roles for Helicases as ATP-Dependent Molecular Switches

scientific article published in January 2013

S-adenosyl homocysteine and DNA ends stimulate promiscuous nuclease activities in the Type III restriction endonuclease EcoPI

scientific article published on 28 April 2009

Sequence-specific assembly of FtsK hexamers establishes directional translocation on DNA ⬇️

scientific article published on November 3, 2010

Single-Molecule Insight Into Target Recognition by CRISPR-Cas Complexes

scientific article published on 5 December 2016

Structural insights into DNA sequence recognition by Type ISP restriction-modification enzymes

scientific article published on 14 March 2016

Subunit assembly modulates the activities of the Type III restriction-modification enzyme PstII in vitro

scientific article

Switching roles for a helicase

scientific article published on 6 September 2013

The Effect of DNA Topology on Observed Rates of R-Loop Formation and DNA Strand Cleavage by CRISPR Cas12a

article

The H-subunit of the restriction endonuclease CglI contains a prototype DEAD-Z1 helicase-like motor.

scientific article published on 20 February 2018

The Type ISP Restriction-Modification enzymes LlaBIII and LlaGI use a translocation-collision mechanism to cleave non-specific DNA distant from their recognition sites.

scientific article

The helicase-like domains of type III restriction enzymes trigger long-range diffusion along DNA

scientific article

The interrelationship of helicase and nuclease domains during DNA translocation by the molecular motor EcoR124I.

scientific article published on 15 October 2008

The single polypeptide restriction-modification enzyme LlaGI is a self-contained molecular motor that translocates DNA loops

scientific article published on November 2009

Translocation, switching and gating: potential roles for ATP in long-range communication on DNA by Type III restriction endonucleases

scientific article

Translocation-coupled DNA cleavage by the Type ISP restriction-modification enzymes

scientific article

Type III restriction endonucleases are heterotrimeric: comprising one helicase-nuclease subunit and a dimeric methyltransferase that binds only one specific DNA.

scientific article published on 7 February 2014

Type III restriction enzymes cleave DNA by long-range interaction between sites in both head-to-head and tail-to-tail inverted repeat

scientific article

When a helicase is not a helicase: dsDNA tracking by the motor protein EcoR124I.

scientific article

List of works by Mark D. Szczelkun

A RecB-family nuclease motif in the Type I restriction endonuclease EcoR124I

A new detection system for extremely small vertically mounted cantilevers

An Mrr-family nuclease motif in the single polypeptide restriction-modification enzyme LlaGI.

CgII cleaves DNA using a mechanism distinct from other ATP-dependent restriction endonucleases

Characterization of the Type III restriction endonuclease PstII from Providencia stuartii

Characterization of the Type III restriction endonuclease PstII from Providencia stuartii.

ClpXP protease targets long-lived DNA translocation states of a helicase-like motor to cause restriction alleviation

Continuous assays for DNA translocation using fluorescent triplex dissociation: application to type I restriction endonucleases.

Controlling the motor activity of a transcription-repair coupling factor: autoinhibition and the role of RNA polymerase

DNA cleavage and methylation specificity of the single polypeptide restriction-modification enzyme LlaGI.

DNA cleavage by CgII and NgoAVII requires interaction between N- and R-proteins and extensive nucleotide hydrolysis

DNA cleavage by Type ISP Restriction-Modification enzymes is initially targeted to the 3'-5' strand

DNA cleavage site selection by Type III restriction enzymes provides evidence for head-on protein collisions following 1D bidirectional motion.

Direct and random routing of a molecular motor protein at a DNA junction

Direct observation of R-loop formation by single RNA-guided Cas9 and Cascade effector complexes.

Dissociation from DNA of Type III Restriction-Modification enzymes during helicase-dependent motion and following endonuclease activity

Dynamics of initiation, termination and reinitiation of DNA translocation by the motor protein EcoR124I

Hexameric assembly of the AAA+ protein McrB is necessary for GTPase activity

How to Build a DNA Unwinding Machine ⬇️

Length heterogeneity at conserved sequence block 2 in human mitochondrial DNA acts as a rheostat for RNA polymerase POLRMT activity

Maintaining a sense of direction during long-range communication on DNA.

Mapping DNA cleavage by the Type ISP restriction-modification enzymes following long-range communication between DNA sites in different orientations

Mitochondrial import, health and mtDNA copy number variability using type II and type V CRISPR effectors

Motor step size and ATP coupling efficiency of the dsDNA translocase EcoR124I.

Re-evaluating the kinetics of ATP hydrolysis during initiation of DNA sliding by Type III restriction enzymes

Recycling of protein subunits during DNA translocation and cleavage by Type I restriction-modification enzymes

Repercussions of DNA tracking by the type IC restriction endonuclease EcoR124I on linear, circular and catenated substrates.

Roles for Helicases as ATP-Dependent Molecular Switches

S-adenosyl homocysteine and DNA ends stimulate promiscuous nuclease activities in the Type III restriction endonuclease EcoPI

Sequence-specific assembly of FtsK hexamers establishes directional translocation on DNA ⬇️

Single-Molecule Insight Into Target Recognition by CRISPR-Cas Complexes

Structural insights into DNA sequence recognition by Type ISP restriction-modification enzymes

Subunit assembly modulates the activities of the Type III restriction-modification enzyme PstII in vitro

Switching roles for a helicase

The Effect of DNA Topology on Observed Rates of R-Loop Formation and DNA Strand Cleavage by CRISPR Cas12a

The H-subunit of the restriction endonuclease CglI contains a prototype DEAD-Z1 helicase-like motor.

The Type ISP Restriction-Modification enzymes LlaBIII and LlaGI use a translocation-collision mechanism to cleave non-specific DNA distant from their recognition sites.

The helicase-like domains of type III restriction enzymes trigger long-range diffusion along DNA

The interrelationship of helicase and nuclease domains during DNA translocation by the molecular motor EcoR124I.

The single polypeptide restriction-modification enzyme LlaGI is a self-contained molecular motor that translocates DNA loops

Translocation, switching and gating: potential roles for ATP in long-range communication on DNA by Type III restriction endonucleases

Translocation-coupled DNA cleavage by the Type ISP restriction-modification enzymes

Type III restriction endonucleases are heterotrimeric: comprising one helicase-nuclease subunit and a dimeric methyltransferase that binds only one specific DNA.

Type III restriction enzymes cleave DNA by long-range interaction between sites in both head-to-head and tail-to-tail inverted repeat

When a helicase is not a helicase: dsDNA tracking by the motor protein EcoR124I.