List of works - Walter Keller

1H, 15N and 13C chemical shift assignment of the Gram-positive conjugative transfer protein TraHpIP501.

scientific article

A hybrid molecule resembling the epitope spectrum of grass pollen for allergy vaccination.

scientific article published in May 2005

A hypoallergenic vaccine obtained by tail-to-head restructuring of timothy grass pollen profilin, Phl p 12, for the treatment of cross-sensitization to profilin

scientific article published in December 2007

Altered IgE epitope presentation: A model for hypoallergenic activity revealed for Bet v 1 trimer.

scientific article published on 18 November 2010

An Engineered Hybrid Protein from Dermatophagoides pteronyssinus Allergens Shows Hypoallergenicity

scientific article published on 21 June 2019

Antiparasitic compounds from Cupania cinerea with activities against Plasmodium falciparum and Trypanosoma brucei rhodesiense

scientific article published on 25 March 2011

Backbone resonance assignment of Alt a 1, a unique β-barrel protein and the major allergen of Alternaria alternata

scientific article

Bacterial fermentation of recombinant major wasp allergen Antigen 5 using oxygen limiting growth conditions improves yield and quality of inclusion bodies

scientific article published on 8 February 2006

Bacterially expressed and optimized recombinant Phl p 1 is immunobiochemically equivalent to natural Phl p 1

scientific article published on 30 September 2006

Biochemical characterization of three putative ATPases from a new type IV secretion system of Aeromonas veronii plasmid pAC3249A

scientific article

Biochemical, biophysical and IgE-epitope characterization of the wheat food allergen, Tri a 37

scientific article

Biophysical characterization of recombinant HIV-1 subtype C virus infectivity factor

scientific article published on 31 August 2010

Carrier-bound nonallergenic Der p 2 peptides induce IgG antibodies blocking allergen-induced basophil activation in allergic patients.

scientific article

Characterisation of recombinant CD23 in the trimeric complex with IgE and allergen

Characterization of Der p 21, a new important allergen derived from the gut of house dust mites.

scientific article

Characterization of a hybrid protein designed with segments of allergens from Blomia tropicalis and Dermatophagoides pteronyssinus

scientific article published on 3 February 2018

Characterization of a hypoallergenic recombinant Bet v 1 variant as a candidate for allergen-specific immunotherapy

scientific article published on 2 October 2007

Characterization of a novel isoform of alpha-nascent polypeptide-associated complex as IgE-defined autoantigen

scientific article (publication date: October 2002)

Characterization of folded recombinant Der p 5, a potential diagnostic marker allergen for house dust mite allergy

scientific article

Circular dichroism analysis of allergens

scientific article published in March 2004

Clustering of conformational IgE epitopes on the major dog allergen Can f 1.

scientific article published on 22 September 2017

Comparison of the specificities of IgG, IgG-subclass, IgA and IgM reactivities in African and European HIV-infected individuals with an HIV-1 clade C proteome-based array

scientific article

Conjugation in Gram-Positive Bacteria

scientific article published on August 2014

Conjugative type IV secretion in Gram-positive pathogens: TraG, a lytic transglycosylase and endopeptidase, interacts with translocation channel protein TraM.

scientific article published on 17 February 2017

Conjugative type IV secretion systems in Gram-positive bacteria

scientific article published on 12 October 2013

Cooking birch pollen-related food: divergent consequences for IgE- and T cell-mediated reactivity in vitro and in vivo.

scientific article

Cor a 14, the allergenic 2S albumin from hazelnut, is highly thermostable and resistant to gastrointestinal digestion

scientific article

Critical and direct involvement of the CD23 stalk region in IgE binding.

scientific article published on 07 May 2016

Crystal structure and immunologic characterization of the major grass pollen allergen Phl p 4.

scientific article published on 14 May 2013

Crystal structure of dimeric HIV-1 capsid protein

scientific article

Crystallization and first data collection of the putative transfer protein TraN from the Gram-positive conjugative plasmid pIP501

scientific article

Crystallization and preliminary structure determination of the C-terminal truncated domain of the S-layer protein SbsC.

scientific article

Crystallization and preliminary structure determination of the plant food allergen Pru av 2.

scientific article published on 8 January 2005

Crystallization and preliminary structure determination of the transfer protein TraM from the Gram-positive conjugative plasmid pIP501

scientific article

Crystallization of domains involved in self-assembly of the S-layer protein SbsC.

scientific article published on 14 November 2012

Different modes of IgE binding to CD23 revealed with major birch allergen, Bet v 1-specific monoclonal IgE.

scientific article

Disruption of allergenic activity of the major grass pollen allergen Phl p 2 by reassembly as a mosaic protein

scientific article published in October 2008

Dissection of the IgE and T-cell recognition of the major group 5 grass pollen allergen Phl p 5.

scientific article published on 31 October 2013

Dissociation of the respiratory syncytial virus F protein-specific human IgG, IgA and IgM response

scientific article published on 11 February 2021

Effect of protamine on the solubility and deamidation of human growth hormone

Epitope specificity determines cross-protection of a SIT-induced IgG4 antibody

scientific article

Expression in Escherichia coli and Purification of Folded rDer p 20, the Arginine Kinase From Dermatophagoides pteronyssinus: A Possible Biomarker for Allergic Asthma

scientific article published on 01 January 2021

Filling the Antibody Pipeline in Allergy: PIPE Cloning of IgE, IgG1 and IgG4 against the Major Birch Pollen Allergen Bet v 1

scientific article published on 08 August 2020

Formation of disulfide bonds and homodimers of the major cat allergen Fel d 1 equivalent to the natural allergen by expression in Escherichia coli.

scientific article

Fusion proteins consisting of Bet v 1 and Phl p 5 form IgE-reactive aggregates with reduced allergenic activity

scientific article published on 08 March 2019

Generation of a low immunoglobulin E-binding mutant of the timothy grass pollen major allergen Phl p 5a.

scientific article published on March 2007

Generation of an allergy vaccine by disruption of the three-dimensional structure of the cross-reactive calcium-binding allergen, Phl p 7.

scientific article published on May 2004

Genetic engineering of the major timothy grass pollen allergen, Phl p 6, to reduce allergenic activity and preserve immunogenicity

scientific article published in August 2007

High-resolution crystal structure and IgE recognition of the major grass pollen allergen Phl p 3.

scientific article published on 6 October 2014

Hypoallergenic derivatives of the major birch pollen allergen Bet v 1 obtained by rational sequence reassembly.

scientific article

Hypoallergenic mutants of the Timothy grass pollen allergen Phl p 5 generated by proline mutations

scientific article published on 30 May 2012

Identification of Der p 23, a peritrophin-like protein, as a new major Dermatophagoides pteronyssinus allergen associated with the peritrophic matrix of mite fecal pellets.

scientific article

IgE Epitopes of the House Dust Mite Allergen Der p 7 Are Mainly Discontinuous and Conformational

scientific article published on 15 June 2021

IgE epitope proximity determines immune complex shape and effector cell activation capacity

scientific article published on 9 December 2015

Isolation of a high-affinity Bet v 1-specific IgG-derived ScFv from a subject vaccinated with hypoallergenic Bet v 1 fragments.

scientific article published on 20 February 2018

Mimotopes identify conformational B-cell epitopes on the two major house dust mite allergens Der p 1 and Der p 2.

scientific article

Misdirected antibody responses against an N-terminal epitope on human rhinovirus VP1 as explanation for recurrent RV infections

scientific article published on 25 November 2011

Mitochondrial lipids in neurodegeneration

scientific article

Molecular characterization of recombinant T1, a non-allergenic periwinkle (Catharanthus roseus) protein, with sequence similarity to the Bet v 1 plant allergen family

scientific article

Molecular, Structural and Immunological Characterization of Der p 18, a Chitinase-Like House Dust Mite Allergen

scientific article

Mutants of the major ryegrass pollen allergen, Lol p 5, with reduced IgE-binding capacity: candidates for grass pollen-specific immunotherapy

scientific article published in January 2002

Natural and recombinant molecules of the cherry allergen Pru av 2 show diverse structural and B cell characteristics but similar T cell reactivity.

scientific article

Neutralization of SARS-CoV-2 requires antibodies against conformational receptor-binding domain epitopes

scientific article published on 22 September 2021

Prediction of IgE-binding epitopes by means of allergen surface comparison and correlation to cross-reactivity

scientific article

Pressurized CO2 as a carboxylating agent for the biocatalytic ortho-carboxylation of resorcinol.

scientific article

Rational design of a hypoallergenic Phl p 7 variant for immunotherapy of polcalcin-sensitized patients

scientific article published on 24 May 2019

Reagent or myeloperoxidase-generated hypochlorite affects discrete regions in lipid-free and lipid-associated human apolipoprotein A-I.

scientific article published in March 2000

Recombinant PreS‐fusion protein vaccine for birch pollen and apple allergy

scientific article published in 2023

Recombinant carp parvalbumin, the major cross-reactive fish allergen: a tool for diagnosis and therapy of fish allergy.

scientific article

Recombinant monoclonal human immunoglobulin E to investigate the allergenic activity of major grass pollen allergen Phl p 5.

scientific article published on 10 December 2010

Reduction of the in vivo allergenicity of Der p 2, the major house-dust mite allergen, by genetic engineering.

scientific article published on 4 March 2008

S-adenosyl-L-homocysteine hydrolase and methylation disorders: yeast as a model system

scientific article published on 24 September 2012

SPADE web service for prediction of allergen IgE epitopes

scientific article published on 01 July 2019

Similar localization of conformational IgE epitopes on the house dust mite allergens Der p 5 and Der p 21 despite limited IgE-cross-reactivity.

scientific article published on 10 January 2018

Structural basis for nucleic acid and toxin recognition of the bacterial antitoxin CcdA.

scientific article

Structure determination of monoclinic canine parvovirus

scientific article published in February 1992

Structure of allergens and structure based epitope predictions.

scientific article published on 23 July 2013

Structure of the double-stranded DNA-binding type IV secretion protein TraN from Enterococcus

scientific article

Targeting Type IV Secretion System Proteins to Combat Multidrug-Resistant Gram-positive Pathogens.

scientific article

The 2.5 A Structure of the Enterococcus Conjugation Protein TraM resembles VirB8 Type IV Secretion Proteins

scientific article

The Coordinated Action of Calcineurin and Cathepsin D Protects Against α-Synuclein Toxicity.

scientific article published on 30 June 2017

The Essential Transfer Protein TraM Binds to DNA as a Tetramer

scientific article published in Journal of Biological Chemistry

The TraA relaxase autoregulates the putative type IV secretion-like system encoded by the broad-host-range Streptococcus agalactiae plasmid pIP501.

scientific article published in March 2006

The high-molecular-mass amylase (HMMA) of Geobacillus stearothermophilus ATCC 12980 interacts with the cell wall components by virtue of three specific binding regions

scientific article

The structure and binding behavior of the bacterial cell surface layer protein SbsC

scientific article

The structure of bacterial S-layer proteins.

scientific article

The type IV secretion protein TraK from the Enterococcus conjugative plasmid pIP501 exhibits a novel fold

scientific article

Thermodynamic properties and DNA binding of the ParD protein from the broad host-range plasmid RK2/RP4 killing system.

scientific article published on December 1999

Three-dimensional structure of the cross-reactive pollen allergen Che a 3: visualizing cross-reactivity on the molecular surfaces of weed, grass, and tree pollen allergens

scientific article

Three-dimensional structure of the panallergen Phl p 7

scientific article published on 01 January 2003

Three-dimensional structure of the wheat β-amylase Tri a 17, a clinically relevant food allergen.

scientific article published on 19 December 2018

Towards the structure of the C-terminal part of the S-layer protein SbsC

scientific article published on 25 September 2009

TraA and its N-terminal relaxase domain of the Gram-positive plasmid pIP501 show specific oriT binding and behave as dimers in solution

scientific article

TraG encoded by the pIP501 type IV secretion system is a two-domain peptidoglycan-degrading enzyme essential for conjugative transfer.

scientific article published on 2 August 2013

Tracking morphologies at the nanoscale: self-assembly of an amphiphilic designer peptide into a double helix superstructure

scientific article published on June 2015

Trimolecular complex between major birch pollen allergen, Bet v 1, monoclonal allergen-specific human IgE and recombinant CD23.

scientific article published on 17 March 2014

VirB8-like protein TraH is crucial for DNA transfer in Enterococcus faecalis

scientific article

Visualization of clustered IgE epitopes on alpha-lactalbumin

scientific article published on 13 May 2010

X-filtering for a range of coupling constants: application to the detection of intermolecular NOEs

scientific article published in February 2003

List of works by Walter Keller

1H, 15N and 13C chemical shift assignment of the Gram-positive conjugative transfer protein TraHpIP501.

A hybrid molecule resembling the epitope spectrum of grass pollen for allergy vaccination.

A hypoallergenic vaccine obtained by tail-to-head restructuring of timothy grass pollen profilin, Phl p 12, for the treatment of cross-sensitization to profilin

Altered IgE epitope presentation: A model for hypoallergenic activity revealed for Bet v 1 trimer.

An Engineered Hybrid Protein from Dermatophagoides pteronyssinus Allergens Shows Hypoallergenicity

Antiparasitic compounds from Cupania cinerea with activities against Plasmodium falciparum and Trypanosoma brucei rhodesiense

Backbone resonance assignment of Alt a 1, a unique β-barrel protein and the major allergen of Alternaria alternata

Bacterial fermentation of recombinant major wasp allergen Antigen 5 using oxygen limiting growth conditions improves yield and quality of inclusion bodies

Bacterially expressed and optimized recombinant Phl p 1 is immunobiochemically equivalent to natural Phl p 1

Biochemical characterization of three putative ATPases from a new type IV secretion system of Aeromonas veronii plasmid pAC3249A

Biochemical, biophysical and IgE-epitope characterization of the wheat food allergen, Tri a 37

Biophysical characterization of recombinant HIV-1 subtype C virus infectivity factor

Carrier-bound nonallergenic Der p 2 peptides induce IgG antibodies blocking allergen-induced basophil activation in allergic patients.

Characterisation of recombinant CD23 in the trimeric complex with IgE and allergen

Characterization of Der p 21, a new important allergen derived from the gut of house dust mites.

Characterization of a hybrid protein designed with segments of allergens from Blomia tropicalis and Dermatophagoides pteronyssinus

Characterization of a hypoallergenic recombinant Bet v 1 variant as a candidate for allergen-specific immunotherapy

Characterization of a novel isoform of alpha-nascent polypeptide-associated complex as IgE-defined autoantigen

Characterization of folded recombinant Der p 5, a potential diagnostic marker allergen for house dust mite allergy

Circular dichroism analysis of allergens

Clustering of conformational IgE epitopes on the major dog allergen Can f 1.

Comparison of the specificities of IgG, IgG-subclass, IgA and IgM reactivities in African and European HIV-infected individuals with an HIV-1 clade C proteome-based array

Conjugation in Gram-Positive Bacteria

Conjugative type IV secretion in Gram-positive pathogens: TraG, a lytic transglycosylase and endopeptidase, interacts with translocation channel protein TraM.

Conjugative type IV secretion systems in Gram-positive bacteria

Cooking birch pollen-related food: divergent consequences for IgE- and T cell-mediated reactivity in vitro and in vivo.

Cor a 14, the allergenic 2S albumin from hazelnut, is highly thermostable and resistant to gastrointestinal digestion

Critical and direct involvement of the CD23 stalk region in IgE binding.

Crystal structure and immunologic characterization of the major grass pollen allergen Phl p 4.

Crystal structure of dimeric HIV-1 capsid protein

Crystallization and first data collection of the putative transfer protein TraN from the Gram-positive conjugative plasmid pIP501

Crystallization and preliminary structure determination of the C-terminal truncated domain of the S-layer protein SbsC.

Crystallization and preliminary structure determination of the plant food allergen Pru av 2.

Crystallization and preliminary structure determination of the transfer protein TraM from the Gram-positive conjugative plasmid pIP501

Crystallization of domains involved in self-assembly of the S-layer protein SbsC.

Different modes of IgE binding to CD23 revealed with major birch allergen, Bet v 1-specific monoclonal IgE.

Disruption of allergenic activity of the major grass pollen allergen Phl p 2 by reassembly as a mosaic protein

Dissection of the IgE and T-cell recognition of the major group 5 grass pollen allergen Phl p 5.

Dissociation of the respiratory syncytial virus F protein-specific human IgG, IgA and IgM response

Effect of protamine on the solubility and deamidation of human growth hormone

Epitope specificity determines cross-protection of a SIT-induced IgG4 antibody

Expression in Escherichia coli and Purification of Folded rDer p 20, the Arginine Kinase From Dermatophagoides pteronyssinus: A Possible Biomarker for Allergic Asthma

Filling the Antibody Pipeline in Allergy: PIPE Cloning of IgE, IgG1 and IgG4 against the Major Birch Pollen Allergen Bet v 1

Formation of disulfide bonds and homodimers of the major cat allergen Fel d 1 equivalent to the natural allergen by expression in Escherichia coli.

Fusion proteins consisting of Bet v 1 and Phl p 5 form IgE-reactive aggregates with reduced allergenic activity

Generation of a low immunoglobulin E-binding mutant of the timothy grass pollen major allergen Phl p 5a.

Generation of an allergy vaccine by disruption of the three-dimensional structure of the cross-reactive calcium-binding allergen, Phl p 7.

Genetic engineering of the major timothy grass pollen allergen, Phl p 6, to reduce allergenic activity and preserve immunogenicity

High-resolution crystal structure and IgE recognition of the major grass pollen allergen Phl p 3.

Hypoallergenic derivatives of the major birch pollen allergen Bet v 1 obtained by rational sequence reassembly.

Hypoallergenic mutants of the Timothy grass pollen allergen Phl p 5 generated by proline mutations

Identification of Der p 23, a peritrophin-like protein, as a new major Dermatophagoides pteronyssinus allergen associated with the peritrophic matrix of mite fecal pellets.

IgE Epitopes of the House Dust Mite Allergen Der p 7 Are Mainly Discontinuous and Conformational

IgE epitope proximity determines immune complex shape and effector cell activation capacity

Isolation of a high-affinity Bet v 1-specific IgG-derived ScFv from a subject vaccinated with hypoallergenic Bet v 1 fragments.

Mimotopes identify conformational B-cell epitopes on the two major house dust mite allergens Der p 1 and Der p 2.

Misdirected antibody responses against an N-terminal epitope on human rhinovirus VP1 as explanation for recurrent RV infections

Mitochondrial lipids in neurodegeneration

Molecular characterization of recombinant T1, a non-allergenic periwinkle (Catharanthus roseus) protein, with sequence similarity to the Bet v 1 plant allergen family

Molecular, Structural and Immunological Characterization of Der p 18, a Chitinase-Like House Dust Mite Allergen

Mutants of the major ryegrass pollen allergen, Lol p 5, with reduced IgE-binding capacity: candidates for grass pollen-specific immunotherapy

Natural and recombinant molecules of the cherry allergen Pru av 2 show diverse structural and B cell characteristics but similar T cell reactivity.

Neutralization of SARS-CoV-2 requires antibodies against conformational receptor-binding domain epitopes

Prediction of IgE-binding epitopes by means of allergen surface comparison and correlation to cross-reactivity

Pressurized CO2 as a carboxylating agent for the biocatalytic ortho-carboxylation of resorcinol.

Rational design of a hypoallergenic Phl p 7 variant for immunotherapy of polcalcin-sensitized patients

Reagent or myeloperoxidase-generated hypochlorite affects discrete regions in lipid-free and lipid-associated human apolipoprotein A-I.

Recombinant PreS‐fusion protein vaccine for birch pollen and apple allergy

Recombinant carp parvalbumin, the major cross-reactive fish allergen: a tool for diagnosis and therapy of fish allergy.

Recombinant monoclonal human immunoglobulin E to investigate the allergenic activity of major grass pollen allergen Phl p 5.

Reduction of the in vivo allergenicity of Der p 2, the major house-dust mite allergen, by genetic engineering.

S-adenosyl-L-homocysteine hydrolase and methylation disorders: yeast as a model system

SPADE web service for prediction of allergen IgE epitopes

Similar localization of conformational IgE epitopes on the house dust mite allergens Der p 5 and Der p 21 despite limited IgE-cross-reactivity.

Structural basis for nucleic acid and toxin recognition of the bacterial antitoxin CcdA.

Structure determination of monoclinic canine parvovirus

Structure of allergens and structure based epitope predictions.

Structure of the double-stranded DNA-binding type IV secretion protein TraN from Enterococcus

Targeting Type IV Secretion System Proteins to Combat Multidrug-Resistant Gram-positive Pathogens.