List of works - Graham D. Pavitt

A Saccharomyces cerevisiae cell-based quantitative beta-galactosidase assay compatible with robotic handling and high-throughput screening

scientific article published in January 2008

A new function and complexity for protein translation initiation factor eIF2B

scientific article published on January 2014

A yeast purification system for human translation initiation factors eIF2 and eIF2Bε and their use in the diagnosis of CACH/VWM disease

scientific article

An eIF5/eIF2 complex antagonizes guanine nucleotide exchange by eIF2B during translation initiation

scientific article

Analysis and reconstitution of translation initiation in vitro

scientific article

Archetypal transcriptional blocks underpin yeast gene regulation in response to changes in growth conditions.

scientific article

Change in nutritional status modulates the abundance of critical pre-initiation intermediate complexes during translation initiation in vivo

scientific article

Characterization of the initiation factor eIF2B and its regulation in Drosophila melanogaster

scientific article

Characterization of the minimal catalytic domain within eIF2B: the guanine-nucleotide exchange factor for translation initiation

scientific article

Chromosomal domains of supercoiling in Salmonella typhimurium

scientific article published in November 1993

Clues to the mechanism of action of eIF2B, the guanine-nucleotide-exchange factor for translation initiation

scientific article published on August 2008

Conserved bipartite motifs in yeast eIF5 and eIF2Bepsilon, GTPase-activating and GDP-GTP exchange factors in translation initiation, mediate binding to their common substrate eIF2.

scientific article

Critical contacts between the eukaryotic initiation factor 2B (eIF2B) catalytic domain and both eIF2beta and -2gamma mediate guanine nucleotide exchange.

scientific article published on 25 May 2007

Discovery of chemical modulators of a conserved translational control pathway by parallel screening in yeast

scientific article published in October 2009

Dynamic changes in eIF4F-mRNA interactions revealed by global analyses of environmental stress responses

scientific article published on 27 October 2017

Enhanced translation initiation factor 4G levels correlate with production levels of monoclonal antibodies in recombinant CHO cell lines

scientific article published in March 2016

Expression and mutational analysis of the nucleoid-associated protein H-NS of Salmonella typhimurium

scientific article

Fail-safe control of translation initiation by dissociation of eIF2α phosphorylated ternary complexes

scientific article

Fusel alcohols regulate translation initiation by inhibiting eIF2B to reduce ternary complex in a mechanism that may involve altering the integrity and dynamics of the eIF2B body.

scientific article

Global gene expression profiling reveals widespread yet distinctive translational responses to different eukaryotic translation initiation factor 2B-targeting stress pathways

scientific article published on November 2005

Global mRNA selection mechanisms for translation initiation.

scientific article

Global translational responses to oxidative stress impact upon multiple levels of protein synthesis.

scientific article published on 18 July 2006

Histone-like protein H1 (H-NS), DNA supercoiling, and gene expression in bacteria

scientific article (publication date: 2 November 1990)

Homologous segments in three subunits of the guanine nucleotide exchange factor eIF2B mediate translational regulation by phosphorylation of eIF2.

scientific article

Identification of intersubunit domain interactions within eukaryotic initiation factor (eIF) 2B, the nucleotide exchange factor for translation initiation

scientific article published on 11 January 2012

Identifying eIF4E-binding protein translationally-controlled transcripts reveals links to mRNAs bound by specific PUF proteins

scientific article

Impaired eIF5A function causes a Mendelian disorder that is partially rescued in model systems by spermidine

scientific article published on 05 February 2021

Integrated multi-omics analyses reveal the pleiotropic nature of the control of gene expression by Puf3p

scientific article

Less translational control, more memory ⬇️

scientific article published on May 28, 2013

Mechanism and Regulation of Protein Synthesis in Saccharomyces cerevisiae

scientific article published on May 2016

Mechanisms of translational regulation by a human eIF5-mimic protein

scientific article

Mutations causing childhood ataxia with central nervous system hypomyelination reduce eukaryotic initiation factor 2B complex formation and activity

scientific article

Mutations in SNORD118 cause the cerebral microangiopathy leukoencephalopathy with calcifications and cysts.

scientific article published on 29 August 2016

New Insights into Translational Regulation in the Endoplasmic Reticulum Unfolded Protein Response ⬇️

scientific article published on June 1, 2012

Parallel High-Throughput Automated Assays to Measure Cell Growth and Beta-Galactosidase Reporter Gene Expression in the Yeast Saccharomyces cerevisiae

scientific article published on March 2011

Protein Synthesis Initiation in Eukaryotic Cells

scientific article published on 03 December 2018

Protein synthesis and its control in neuronal cells with a focus on vanishing white matter disease

scientific article published on December 2009

Puf3p induces translational repression of genes linked to oxidative stress

scientific article published on 25 October 2013

Purification and kinetic analysis of eIF2B from Saccharomyces cerevisiae

scientific article published on 01 August 2000

Purification of FLAG-tagged eukaryotic initiation factor 2B complexes, subcomplexes, and fragments from Saccharomyces cerevisiae

scientific article published on January 2007

Regulation of translation initiation factor eIF2B at the hub of the integrated stress response

scientific article published on 10 July 2018

Structure of the catalytic fragment of translation initiation factor 2B and identification of a critically important catalytic residue

scientific article

The 4E-BP Caf20p Mediates Both eIF4E-Dependent and Independent Repression of Translation

scientific article published on 14 May 2015

The alpha subunit of eukaryotic initiation factor 2B (eIF2B) is required for eIF2-mediated translational suppression of vesicular stomatitis virus

scientific article published on 27 July 2011

The chromatin-associated protein H-NS interacts with curved DNA to influence DNA topology and gene expression

scientific article (publication date: 16 October 1992)

The structural basis of translational control by eIF2 phosphorylation

scientific article published on 13 May 2019

The yeast La related protein Slf1p is a key activator of translation during the oxidative stress response.

scientific article

Translation controlled.

scientific article published on 21 October 2008

Translation reprogramming is an evolutionarily conserved driver of phenotypic plasticity and therapeutic resistance in melanoma.

scientific article published on 17 January 2017

Translational regulation in response to stress in Saccharomyces cerevisiae

scientific article published on 03 September 2018

Upstream sequence elements direct post-transcriptional regulation of gene expression under stress conditions in yeast.

scientific article

eIF2 independently binds two distinct eIF2B subcomplexes that catalyze and regulate guanine-nucleotide exchange.

scientific article

eIF2B is a decameric guanine nucleotide exchange factor with a γ2ε2 tetrameric core

scientific article

eIF2B promotes eIF5 dissociation from eIF2*GDP to facilitate guanine nucleotide exchange for translation initiation

scientific article published on December 2013

eIF2β is critical for eIF5-mediated GDP-dissociation inhibitor activity and translational control

scientific article published on 25 July 2016

eIF5 has GDI activity necessary for translational control by eIF2 phosphorylation

scientific article

eIF5 is a dual function GAP and GDI for eukaryotic translational control

scientific article published on September 2010

List of works by Graham D. Pavitt

A Saccharomyces cerevisiae cell-based quantitative beta-galactosidase assay compatible with robotic handling and high-throughput screening

A new function and complexity for protein translation initiation factor eIF2B

A yeast purification system for human translation initiation factors eIF2 and eIF2Bε and their use in the diagnosis of CACH/VWM disease

An eIF5/eIF2 complex antagonizes guanine nucleotide exchange by eIF2B during translation initiation

Analysis and reconstitution of translation initiation in vitro

Archetypal transcriptional blocks underpin yeast gene regulation in response to changes in growth conditions.

Change in nutritional status modulates the abundance of critical pre-initiation intermediate complexes during translation initiation in vivo

Characterization of the initiation factor eIF2B and its regulation in Drosophila melanogaster

Characterization of the minimal catalytic domain within eIF2B: the guanine-nucleotide exchange factor for translation initiation

Chromosomal domains of supercoiling in Salmonella typhimurium

Clues to the mechanism of action of eIF2B, the guanine-nucleotide-exchange factor for translation initiation

Conserved bipartite motifs in yeast eIF5 and eIF2Bepsilon, GTPase-activating and GDP-GTP exchange factors in translation initiation, mediate binding to their common substrate eIF2.

Critical contacts between the eukaryotic initiation factor 2B (eIF2B) catalytic domain and both eIF2beta and -2gamma mediate guanine nucleotide exchange.

Discovery of chemical modulators of a conserved translational control pathway by parallel screening in yeast

Dynamic changes in eIF4F-mRNA interactions revealed by global analyses of environmental stress responses

Enhanced translation initiation factor 4G levels correlate with production levels of monoclonal antibodies in recombinant CHO cell lines

Expression and mutational analysis of the nucleoid-associated protein H-NS of Salmonella typhimurium

Fail-safe control of translation initiation by dissociation of eIF2α phosphorylated ternary complexes

Fusel alcohols regulate translation initiation by inhibiting eIF2B to reduce ternary complex in a mechanism that may involve altering the integrity and dynamics of the eIF2B body.

Global gene expression profiling reveals widespread yet distinctive translational responses to different eukaryotic translation initiation factor 2B-targeting stress pathways

Global mRNA selection mechanisms for translation initiation.

Global translational responses to oxidative stress impact upon multiple levels of protein synthesis.

Histone-like protein H1 (H-NS), DNA supercoiling, and gene expression in bacteria

Homologous segments in three subunits of the guanine nucleotide exchange factor eIF2B mediate translational regulation by phosphorylation of eIF2.

Identification of intersubunit domain interactions within eukaryotic initiation factor (eIF) 2B, the nucleotide exchange factor for translation initiation

Identifying eIF4E-binding protein translationally-controlled transcripts reveals links to mRNAs bound by specific PUF proteins

Impaired eIF5A function causes a Mendelian disorder that is partially rescued in model systems by spermidine

Integrated multi-omics analyses reveal the pleiotropic nature of the control of gene expression by Puf3p

Less translational control, more memory ⬇️

Mechanism and Regulation of Protein Synthesis in Saccharomyces cerevisiae

Mechanisms of translational regulation by a human eIF5-mimic protein

Mutations causing childhood ataxia with central nervous system hypomyelination reduce eukaryotic initiation factor 2B complex formation and activity

Mutations in SNORD118 cause the cerebral microangiopathy leukoencephalopathy with calcifications and cysts.

New Insights into Translational Regulation in the Endoplasmic Reticulum Unfolded Protein Response ⬇️

Parallel High-Throughput Automated Assays to Measure Cell Growth and Beta-Galactosidase Reporter Gene Expression in the Yeast Saccharomyces cerevisiae

Protein Synthesis Initiation in Eukaryotic Cells

Protein synthesis and its control in neuronal cells with a focus on vanishing white matter disease

Puf3p induces translational repression of genes linked to oxidative stress

Purification and kinetic analysis of eIF2B from Saccharomyces cerevisiae

Purification of FLAG-tagged eukaryotic initiation factor 2B complexes, subcomplexes, and fragments from Saccharomyces cerevisiae

Regulation of translation initiation factor eIF2B at the hub of the integrated stress response

Structure of the catalytic fragment of translation initiation factor 2B and identification of a critically important catalytic residue

The 4E-BP Caf20p Mediates Both eIF4E-Dependent and Independent Repression of Translation

The alpha subunit of eukaryotic initiation factor 2B (eIF2B) is required for eIF2-mediated translational suppression of vesicular stomatitis virus

The chromatin-associated protein H-NS interacts with curved DNA to influence DNA topology and gene expression

The structural basis of translational control by eIF2 phosphorylation

The yeast La related protein Slf1p is a key activator of translation during the oxidative stress response.

Translation controlled.

Translation reprogramming is an evolutionarily conserved driver of phenotypic plasticity and therapeutic resistance in melanoma.

Translational regulation in response to stress in Saccharomyces cerevisiae

Upstream sequence elements direct post-transcriptional regulation of gene expression under stress conditions in yeast.

eIF2 independently binds two distinct eIF2B subcomplexes that catalyze and regulate guanine-nucleotide exchange.

eIF2B is a decameric guanine nucleotide exchange factor with a γ2ε2 tetrameric core

eIF2B promotes eIF5 dissociation from eIF2*GDP to facilitate guanine nucleotide exchange for translation initiation

eIF2β is critical for eIF5-mediated GDP-dissociation inhibitor activity and translational control

eIF5 has GDI activity necessary for translational control by eIF2 phosphorylation

eIF5 is a dual function GAP and GDI for eukaryotic translational control